{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T23:26:44Z","timestamp":1771975604516,"version":"3.50.1"},"reference-count":71,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T00:00:00Z","timestamp":1612828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"RUSSIAN ACADEMIC SCIENCE EXCELLENCE PROJECT","award":["\"5-100\""],"award-info":[{"award-number":["\"5-100\""]}]},{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["19-315-90058"],"award-info":[{"award-number":["19-315-90058"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"name":"A STATE ASSIGNMENT 0082-2019-0012","award":["State registration number \u0410\u0410\u0410\u0410-\u041020-120021190043-7"],"award-info":[{"award-number":["State registration number \u0410\u0410\u0410\u0410-\u041020-120021190043-7"]}]},{"name":"THE SCHOLARSHIP OF THE PRESIDENT OF THE RUSSIAN FEDERATION","award":["(awardee: A. Fayzullin)"],"award-info":[{"award-number":["(awardee: A. Fayzullin)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Mature hypertrophic scars (HSs) remain a challenging clinical problem, particularly due to the absence of biologically relevant experimental models as a standard rabbit ear HS model only reflects an early stage of scarring. The current study aims to adapt this animal model for simulation of mature HS by validating the time of the scar stabilization using qualitative and quantitative criteria. The full-thickness skin and perichondrium excision wounds were created on the ventral side of the rabbit ears. The tissue samples were studied on post-operation days (PODs) 30, 60, 90 and 120. The histopathological examination and morphometry were applied in parallel with biochemical analysis of protein and glycosaminoglycans (GAGs) content and amino acid composition. The supramolecular organization of collagen was explored by differential scanning calorimetry. Four stages of the rabbit ear HS maturation were delineated and attributed with the histolomorphometrical and physicochemical parameters of the tissue. The experimental scars formed in 30 days but stabilized structurally and biochemically only on POD 90\u2013120. This evidence-based model can be used for the studies and testing of new treatments of the mature HSs.<\/jats:p>","DOI":"10.3390\/biology10020136","type":"journal-article","created":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T23:43:16Z","timestamp":1612914196000},"page":"136","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Modeling of Old Scars: Histopathological, Biochemical and Thermal Analysis of the Scar Tissue Maturation"],"prefix":"10.3390","volume":"10","author":[{"given":"Alexey","family":"Fayzullin","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Morphology, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"},{"name":"World-Class Research Center \u201cDigital Biodesign and Personalized Healthcare\u201d, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Ignatieva","sequence":"additional","affiliation":[{"name":"Chemistry Department, Lomonosov Moscow State University, Leninskiye Gory 1-3, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Zakharkina","sequence":"additional","affiliation":[{"name":"Institute of Photon Technologies, Federal Scientific Research Centre \u201cCrystallography and Photonics\u201d of Russian Academy of Sciences, 2 Pionerskaya st., Troitsk, Moscow 142190, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Tokarev","sequence":"additional","affiliation":[{"name":"Sklifosovsky Institute for Clinical Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6570-3909","authenticated-orcid":false,"given":"Daniil","family":"Mudryak","sequence":"additional","affiliation":[{"name":"Sklifosovsky Institute for Clinical Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yana","family":"Khristidis","sequence":"additional","affiliation":[{"name":"World-Class Research Center \u201cDigital Biodesign and Personalized Healthcare\u201d, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxim","family":"Balyasin","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Morphology, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandr","family":"Kurkov","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Morphology, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"},{"name":"World-Class Research Center \u201cDigital Biodesign and Personalized Healthcare\u201d, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3549-5683","authenticated-orcid":false,"given":"Semyon","family":"Churbanov","sequence":"additional","affiliation":[{"name":"Institute of Photon Technologies, Federal Scientific Research Centre \u201cCrystallography and Photonics\u201d of Russian Academy of Sciences, 2 Pionerskaya st., Troitsk, Moscow 142190, Russia"},{"name":"Department of Advanced Biomaterials, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana","family":"Dyuzheva","sequence":"additional","affiliation":[{"name":"Sklifosovsky Institute for Clinical Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Timashev","sequence":"additional","affiliation":[{"name":"Chemistry Department, Lomonosov Moscow State University, Leninskiye Gory 1-3, Moscow 119991, Russia"},{"name":"Institute of Photon Technologies, Federal Scientific Research Centre \u201cCrystallography and Photonics\u201d of Russian Academy of Sciences, 2 Pionerskaya st., Troitsk, Moscow 142190, Russia"},{"name":"Department of Advanced Biomaterials, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"},{"name":"Laboratory of Clinical Smart Nanotechnologies, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"},{"name":"Department of Polymers and Composites, N. N. Semenov Institute of Chemical Physics, 4 Kosygin st., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8866-9838","authenticated-orcid":false,"given":"Anna","family":"Guller","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Morphology, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"},{"name":"ARC Centre of Excellence for Nanoscale Biophotonics, Graduate School of Biomedical Engineering, University of New South Wales, Kensington, NSW 2052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anatoly","family":"Shekhter","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Morphology, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya st., Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/j.1524-4725.2008.34406.x","article-title":"Hypertrophic scars and keloids\u2013a review of their pathophysiology, risk factors, and therapeutic management","volume":"35","author":"Wolfram","year":"2009","journal-title":"Dermatol. 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