{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T20:05:21Z","timestamp":1793909121506,"version":"4.8.0"},"reference-count":53,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T00:00:00Z","timestamp":1623024000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Leptospirosis is a widespread zoonosis recognized as a re-emerging infectious disease in a wide variety of animal species, including humans and dogs. No data exist regarding the presence of Leptospira species in the canine population of Sardinia Island. This study reports the first sero-survey for leptospirosis in kennel and owned dogs from six areas of the north of Sardinia. Sera from 1296 dogs were tested by microscopic agglutination test (MAT) specific for nine different serovars that are known to be well widespread in the Mediterranean environment. Moreover, kidney homogenates from rodents collected from the study area were also analyzed by LipL32 real-time PCR and multi-locus sequence type (MLST) on the basis of the analysis of seven concatenated loci. A total of 13% of the examined sera (95%CI: 11\u201315) tested positive for one or more serovars of Leptospira MAT detected; antibodies for serogroup Icterohaemorrhagiae (57%; 95%CI: 49\u201365) were the most common, followed by serovars Bratislava (22%; 95%CI: 16\u201328), Canicola (14%; 95%CI: 9\u201319), and Grippotyphosa (7%; 95%CI: 3\u201311). MLST analyses on isolates from rodents identified L. interrogans and L. borgpetersenii genomospecies. Different serovars belonging to pathogenic Leptospira serogroups are circulating in dogs from the island. Moreover, data obtained from rodents, indicated that rodents likely act as reservoir of spirochetes. Further sero-epidemiological studies are needed in order to obtain data from other collection sites in Sardinia and to increase the information on Leptospira species circulating in this area.<\/jats:p>","DOI":"10.3390\/biology10060507","type":"journal-article","created":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T13:25:43Z","timestamp":1623072343000},"page":"507","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["New Insights on Leptospira Infections in a Canine Population from North Sardinia, Italy: A Sero-Epidemiological Study"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2878-4682","authenticated-orcid":false,"given":"Ivana","family":"Piredda","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sardegna, Department of Animal Health, Laboratory of Seroimmunology, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Nicoletta","family":"Ponti","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sardegna, Department of Animal Health, Laboratory of Seroimmunology, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Piras","sequence":"additional","affiliation":[{"name":"Azienda Socio Sanitaria Locale, Azienda Tutela Salute, 09047 Cagliari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna","family":"Palmas","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sardegna, Department of Animal Health, Laboratory of Seroimmunology, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierangela","family":"Pintore","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sardegna, Department of Animal Health, Laboratory of Seroimmunology, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aureliana","family":"Pedditzi","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sardegna, Department of Animal Health, Laboratory of Seroimmunology, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3907-0463","authenticated-orcid":false,"given":"Valentina","family":"Chisu","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sardegna, Department of Animal Health, Laboratory of Seroimmunology, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Cilia, G., Bertelloni, F., and Fratini, F. (2020). Leptospira Infections in Domestic and Wild Animals. Pathogens, 9.","DOI":"10.3390\/pathogens9070573"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Cilia, G., Bertelloni, F., Albini, S., and Fratini, F. (2021). Insight into the Epidemiology of Leptospirosis: A Review of Leptospira Isolations from \u201cUnconventional\u201d Hosts. Animals, 11.","DOI":"10.3390\/ani11010191"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ebani, V.V. (2017). Domestic reptiles as source of zoonotic bacteria: A mini review. Asian Pac. J. Trop. Med.","DOI":"10.1016\/j.apjtm.2017.07.020"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s11250-017-1429-y","article-title":"A systematic review of leptospirosis on wild animals in Latin America","volume":"50","author":"Vieira","year":"2018","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Piredda, I., Palmas, B., Noworol, M., Tola, S., Longheu, C., Bertasio, C., Scaltriti, E., Denurra, D., Cherchi, M., and Picardeau, M. (2020). Isolation of Leptospira interrogans from a Bottlenose Dolphin (Tursiops truncatus) in the Mediterranean Sea. J. Wildl. Dis.","DOI":"10.7589\/2019-07-186"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Loffler, S.G., Rago, V., Martinez, M., Uhart, M., Florin-Christensen, M., Romero, G., and Brihuega, B. (2015). Isolation of a seawater tolerant Leptospira spp. from a southern right whale (Eubalaena australis). PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0144974"},{"key":"ref_7","first-page":"65","article-title":"Leptospirosis in humans","volume":"387","author":"Haake","year":"2015","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Miotto, B.A., Guilloux, A.G.A., Tozzi, B.F., Moreno, L.Z., Da Hora, A.S., Dias, R.A., Heinemann, M.B., Moreno, A.M., de Souza Filho, A.F., and Lilenbaum, W. (2018). Prospective study of canine leptospirosis in shelter and stray dog populations: Identification of chronic carriers and different Leptospira species infecting dogs. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0200384"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"s12917-s020","DOI":"10.1186\/s12917-020-2230-0","article-title":"Leptospira infection and shedding in dogs in Thailand","volume":"16","author":"Altheimer","year":"2020","journal-title":"BMC Vet. Res."},{"key":"ref_10","first-page":"33","article-title":"Seroprevalence and Associated Risk Factors of Canine Leptospira and Brucella Species Infection in West Shewa Zone, Central Ethiopia","volume":"Volume 12","author":"Marami","year":"2021","journal-title":"Vet. Med. Res. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1007\/s10096-010-0991-2","article-title":"Detection and quantification of leptospires in urine of dogs: A maintenance host for the zoonotic disease leptospirosis","volume":"29","author":"Rojas","year":"2010","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.meegid.2010.01.001","article-title":"Leptospira wolffii, a potential new pathogenic Leptospira species detected in human, sheep and dog","volume":"10","author":"Zakeri","year":"2010","journal-title":"Infect. Genet. Evol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4316","DOI":"10.3390\/ijerph110404316","article-title":"Though not reservoirs, dogs might transmit Leptospira in New Caledonia","volume":"11","author":"Gay","year":"2014","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_14","first-page":"251","article-title":"Urinary shedding of leptospires and presence of Leptospira antibodies in healthy dogs from Upper Bavaria","volume":"129","author":"Llewellyn","year":"2016","journal-title":"Berl. Munch. Tierarztl. Wochenschr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1590\/0037-8682-0102-2015","article-title":"Leptospirosis in animals and human contacts in Egypt: Broad range surveillance","volume":"48","author":"Samir","year":"2015","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"s41598-s021","DOI":"10.1038\/s41598-021-82290-5","article-title":"Human, animal, water source interactions and leptospirosis in Thailand","volume":"11","author":"Narkkul","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1111\/jsap.12328","article-title":"European consensus statement on leptospirosis in dogs and cats","volume":"56","author":"Schuller","year":"2015","journal-title":"J. Small Anim. Pract."},{"key":"ref_18","first-page":"781","article-title":"Carriage of pathogenic Leptospira in carnivores at the wild\/domestic interface","volume":"22","author":"Velarde","year":"2019","journal-title":"Pol. J. Vet. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Samrot, A.V., Sean, T.C., Bhavya, K.S., Sahithya, C.S., Chandrasekaran, S., Palanisamy, R., Robinson, E.R., Subbiah, S.K., and Mok, P.L. (2021). Leptospiral infection, pathogenesis and its diagnosis\u2014A review. Pathogens, 10.","DOI":"10.3390\/pathogens10020145"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/j.cvsm.2019.02.008","article-title":"Diagnosis of Canine Leptospirosis","volume":"49","author":"Reagan","year":"2019","journal-title":"Vet. Clin. North Am. Small Anim. Pract."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Bertasio, C., Papetti, A., Scaltriti, E., Tagliabue, S., D\u2019incau, M., and Boniotti, M.B. (2020). Serological survey and molecular typing reveal new Leptospira serogroup pomona strains among pigs of northern Italy. Pathogens, 9.","DOI":"10.3390\/pathogens9050332"},{"key":"ref_22","first-page":"s12917-s017","article-title":"Refinement of intraperitoneal injection of sodium pentobarbital for euthanasia in laboratory rats (Rattus norvegicus)","volume":"13","author":"Zatroch","year":"2017","journal-title":"BMC Vet. Res."},{"key":"ref_23","unstructured":"Nagorsen, D.W. (2002). An Identification Manual to The Small Mammals of British Columbia, Ministry of Sustainable Resource Management Ministry of Water, Land and Air Protection Royal British Columbia Museum."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Piredda, I., Ponti, M.N., Palmas, B., Noworol, M., Pedditzi, A., Rebechesu, L., and Chisu, V. (2021). Molecular Typing of Pathogenic Leptospira Species Isolated from Wild Mammal Reservoirs in Sardinia. Animals, 11.","DOI":"10.3390\/ani11041109"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.diagmicrobio.2009.03.014","article-title":"Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene","volume":"64","author":"Stoddard","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Boonsilp, S., Thaipadungpanit, J., Amornchai, P., Wuthiekanun, V., Bailey, M.S., Holden, M.T.G., Zhang, C., Jiang, X., Koizumi, N., and Taylor, K. (2013). A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0001954"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Balboni, A., Zamagni, S., Bertasio, C., Boniotti, M.B., Tro\u00eca, R., Battilani, M., and Dondi, F. (2020). Identification of Serogroups Australis and Icterohaemorrhagiae in Two Dogs with a Severe Form of Acute Leptospirosis in Italy. Pathogens, 9.","DOI":"10.3390\/pathogens9050351"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1111\/tbed.13110","article-title":"Leptospira infection and shedding in cats in Thailand","volume":"66","author":"Jongwattanapisan","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"s11250-s019","DOI":"10.1007\/s11250-019-01859-5","article-title":"Epidemiology of Leptospira infection in livestock species in Saint Kitts","volume":"51","author":"Shiokawa","year":"2019","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.vetmic.2016.05.004","article-title":"Leptospira interrogans serovars Bratislava and Muenchen animal infections: Implications for epidemiology and control","volume":"190","author":"Arent","year":"2016","journal-title":"Vet. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1111\/evj.13069","article-title":"Leptospirosis: An important infectious disease in North American horses","volume":"51","author":"Divers","year":"2019","journal-title":"Equine Vet. J."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Vera, E., Taddei, S., Cavirani, S., Schiavi, J., Angelone, M., Cabassi, C.S., Schiano, E., and Quintavalla, F. (2020). Leptospira seroprevalence in bardigiano horses in northern Italy. Animals, 10.","DOI":"10.3390\/ani10010023"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"L\u00f3pez, M.C., Vila, A., Rod\u00f3n, J., and Roura, X. (2019). Leptospira seroprevalence in owned dogs from Spain. Heliyon, 5.","DOI":"10.1016\/j.heliyon.2019.e02373"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1177\/1098612X16634389","article-title":"Detection of Leptospira DNA in urine and presence of specific antibodies in outdoor cats in Germany","volume":"19","author":"Weis","year":"2017","journal-title":"J. Feline Med. Surg."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1053\/j.tcam.2017.12.001","article-title":"Seroprevalence of Leptospirosis in Working Dogs","volume":"32","author":"Lau","year":"2017","journal-title":"Top. Companion Anim. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1111\/jvim.12337","article-title":"Vaccine-associated Leptospira antibodies in client-owned dogs","volume":"28","author":"Martin","year":"2014","journal-title":"J. Vet. Intern. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1111\/jvim.12335","article-title":"Regional and temporal variations of Leptospira seropositivity in dogs in the United States, 2000-2010","volume":"28","author":"Lee","year":"2014","journal-title":"J. Vet. Intern. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.2460\/javma.247.10.1139","article-title":"Incidence rates and risk factors for owner-reported adverse events following vaccination of dogs that did or did not receive a Leptospira vaccine","volume":"247","author":"Yao","year":"2015","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"612","DOI":"10.3390\/ani4040612","article-title":"Leptospira spp. in domestic cats from different environments: Prevalence of antibodies and risk factors associated with the seropositivity","volume":"4","author":"Monti","year":"2014","journal-title":"Animals"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Goris, M.G.A., Kikken, V., Straetemans, M., Alba, S., Goeijenbier, M., van Gorp, E.C.M., Boer, K.R., Wagenaar, J.F.P., and Hartskeerl, R.A. (2013). Towards the Burden of Human Leptospirosis: Duration of Acute Illness and Occurrence of Post-Leptospirosis Symptoms of Patients in The Netherlands. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0076549"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"De Brito, T., da Silva, A.M.G., and Abreu, P.A.E. (2018). Pathology and pathogenesis of human leptospirosis: A commented review. Rev. Inst. Med. Trop. Sao Paulo, 60.","DOI":"10.1590\/s1678-9946201860023"},{"key":"ref_42","first-page":"129","article-title":"Indagine sierologica sulla presenza di Leptospira spp. in Italia: Dati nazionali 2010\u20132011","volume":"52","author":"Tagliabue","year":"2016","journal-title":"Vet. Ital."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Bertasio, C., Boniotti, M.B., Lucchese, L., Ceglie, L., Bellinati, L., Mazzucato, M., Furlanello, T., D\u2019incau, M., and Natale, A. (2020). Detection of new Leptospira genotypes infecting symptomatic dogs: Is a new vaccine formulation needed?. Pathogens, 9.","DOI":"10.3390\/pathogens9060484"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1080\/23744235.2020.1739745","article-title":"Epidemiology, clinical and laboratory findings of leptospirosis in Southwestern Greece","volume":"52","author":"Gkentzi","year":"2020","journal-title":"Infect. Dis. (Auckl)."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1093\/infdis\/jiz339","article-title":"Seroprevalence, Risk Factors, and Rodent Reservoirs of Leptospirosis in an Urban Community of Puerto Rico, 2015","volume":"220","author":"Briskin","year":"2019","journal-title":"J. Infect. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"s12917-s015","DOI":"10.1186\/s12917-015-0341-9","article-title":"Seroepidemiology of leptospirosis in dogs from rural and slum communities of Los Rios Region, Chile","volume":"11","author":"Lelu","year":"2015","journal-title":"BMC Vet. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S1473-3099(03)00830-2","article-title":"Leptospirosis: A zoonotic disease of global importance","volume":"3","author":"Bharti","year":"2003","journal-title":"Lancet Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.tvjl.2012.10.002","article-title":"Prevalence of the Leptospira serovars bratislava, grippotyphosa, mozdok and pomona in French dogs","volume":"196","author":"Renaud","year":"2013","journal-title":"Vet. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/0035-9203(82)90270-X","article-title":"Human leptospirosis in somalia: A serological survey","volume":"76","author":"Cacciapuoti","year":"1982","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"756","DOI":"10.4269\/ajtmh.13-0416","article-title":"Short report: Distribution of Leptospira serogroups in cattle herds and dogs in France","volume":"91","author":"Ayral","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7554\/eLife.64166","article-title":"A live attenuated-vaccine model confers cross-protective immunity against different species of the Leptospira genus","volume":"10","author":"Wunder","year":"2021","journal-title":"Elife"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1007\/s10096-019-03797-4","article-title":"Leptospirosis: A neglected tropical zoonotic infection of public health importance\u2014An updated review","volume":"39","author":"Karpagam","year":"2020","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1089\/vbz.2019.2549","article-title":"Investigation of Leptospira infection in stray animals in Songkhla, Thailand: Leptospirosis Risk Reduction in Human","volume":"20","author":"Ngasaman","year":"2020","journal-title":"Vector-Borne Zoonotic Dis."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/10\/6\/507\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:11:41Z","timestamp":1760163101000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/10\/6\/507"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,7]]},"references-count":53,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["biology10060507"],"URL":"https:\/\/doi.org\/10.3390\/biology10060507","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,7]]}}}