{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:28:15Z","timestamp":1791822495238,"version":"4.3.4"},"reference-count":74,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,9,11]],"date-time":"2021-09-11T00:00:00Z","timestamp":1631318400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jewish Charitable Association (ICA) \u2013 Galilee Research Institute (Migal) accelerator, Israel","award":["2020"],"award-info":[{"award-number":["2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Late wilt disease (LWD) is a destructive vascular disease of maize (Zea mays L.) caused by the fungus Magnaporthiopsis maydis. Restricting the disease, which is a significant threat to commercial production in Israel, Egypt, Spain, India, and other countries, is an urgent need. In the past three years, we scanned nine Trichoderma spp. isolates as biological control candidates against M. maydis. Three of these isolates showed promising results. In vitro assays, seedlings pathogenicity trials, and field experiments all support the bio-control potential of these isolates (or their secretions). Here, a dedicated effort led to the isolation and identification of an active ingredient in the growth medium of Trichoderma asperellum (P1) with antifungal activity against M. maydis. This Trichoderma species is an endophyte isolated from LWD-susceptible maize seeds. From the chloroform extract of this fungal medium, we isolated a powerful (approx. 400 mg\/L) active ingredient capable of fully inhibiting M. maydis growth. Additional purification using liquid chromatography\u2013mass spectrometry (LC\u2013MS) and gas chromatography\u2013mass spectrometry (GC\u2013MS) separation steps enabled identifying the active ingredient as 6-Pentyl-\u03b1-pyrone. This compound is a potential fungicide with high efficiency against the LWD causal agent.<\/jats:p>","DOI":"10.3390\/biology10090897","type":"journal-article","created":{"date-parts":[[2021,9,12]],"date-time":"2021-09-12T21:45:57Z","timestamp":1631483157000},"page":"897","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Trichoderma asperellum Secreted 6-Pentyl-\u03b1-Pyrone to Control Magnaporthiopsis maydis, the Maize Late Wilt Disease Agent"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6204-9406","authenticated-orcid":false,"given":"Ofir","family":"Degani","sequence":"first","affiliation":[{"name":"Migal\u2014Galilee Research Institute, Tarshish 2, Kiryat Shmona 11016, Israel"},{"name":"Faculty of Sciences, Tel-Hai College, Upper Galilee, Tel-Hai 12210, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6762-2408","authenticated-orcid":false,"given":"Soliman","family":"Khatib","sequence":"additional","affiliation":[{"name":"Migal\u2014Galilee Research Institute, Tarshish 2, Kiryat Shmona 11016, Israel"},{"name":"Faculty of Sciences, Tel-Hai College, Upper Galilee, Tel-Hai 12210, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paz","family":"Becher","sequence":"additional","affiliation":[{"name":"Migal\u2014Galilee Research Institute, Tarshish 2, Kiryat Shmona 11016, Israel"},{"name":"Faculty of Sciences, Tel-Hai College, Upper Galilee, Tel-Hai 12210, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asaf","family":"Gordani","sequence":"additional","affiliation":[{"name":"Migal\u2014Galilee Research Institute, Tarshish 2, Kiryat Shmona 11016, Israel"},{"name":"Faculty of Sciences, Tel-Hai College, Upper Galilee, Tel-Hai 12210, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raviv","family":"Harris","sequence":"additional","affiliation":[{"name":"Migal\u2014Galilee Research Institute, Tarshish 2, Kiryat Shmona 11016, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1094\/PDIS-05-18-0759-RE","article-title":"Evaluating Azoxystrobin Seed Coating Against Maize Late Wilt Disease Using a Sensitive qPCR-Based Method","volume":"103","author":"Degani","year":"2019","journal-title":"Plant Dis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Kamara, M.M., Ghazy, N.A., Mansour, E., Elsharkawy, M.M., Kheir, A.M.S., and Ibrahim, K.M. 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