{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T19:33:20Z","timestamp":1788896000773,"version":"build-2803163510"},"reference-count":86,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,30]],"date-time":"2022-01-30T00:00:00Z","timestamp":1643500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001872","name":"Centre for Industrial Technological Development","doi-asserted-by":"publisher","award":["CDTI-IDI-20180254"],"award-info":[{"award-number":["CDTI-IDI-20180254"]}],"id":[{"id":"10.13039\/501100001872","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Multiple ovulation and embryo transfer (MOET) systems have been intensively implemented in Japanese Black cattle in Japan and to create Japanese Black herds out of these areas. Environmental conditions influence MOET efficiency. Thus, we describe results of 137 in vivo, non-surgical embryo flushings performed between 2016\u20132020, in a full-blood Japanese Black herd kept in Spain and the possible effects of heat, year, bull, donor genetic value, and metabolic condition. Additionally, 687 embryo transfers were studied for conception rate (CR) and recipient related factors. A total of 71.3% of viable embryos (724\/1015) were obtained (5.3 \u00b1 4.34\/flushing). Donor metabolites did not affect embryo production (p &gt; 0.1), although metabolite differences were observed over the years, and by flushing order, probably related to the donor age. CR was not affected by embryo type (fresh vs. frozen), recipient breed, and whether suckling or not suckling (p &gt; 0.1). CR decreased significantly with heat (44.3 vs. 49.2%; (p = 0.042)) and numerically increased with recipient parity and ET-number. Pregnant recipients showed significantly higher levels of cholesterol-related metabolites, glucose, and urea (p &lt; 0.05). Therefore, adequate MOET efficiency can be achieved under these conditions, and heat stress should be strongly avoided during Japanese Black embryo transfers. Moreover, recipients\u2019 metabolites are important to achieve pregnancy, being probably related to better nutrient availability during pregnancy.<\/jats:p>","DOI":"10.3390\/biology11020225","type":"journal-article","created":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T01:46:08Z","timestamp":1643593568000},"page":"225","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["MOET Efficiency in a Spanish Herd of Japanese Black Heifers and Analysis of Environmental and Metabolic Determinants"],"prefix":"10.3390","volume":"11","author":[{"given":"Juan M.","family":"V\u00e1zquez-Mosquera","sequence":"first","affiliation":[{"name":"Medicine and Surgery Department, Veterinary Faculty, Complutense University of Madrid, Puerta de Hierro Avenue s\/n, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0112-8543","authenticated-orcid":false,"given":"Aitor","family":"Fern\u00e1ndez-Novo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, School of Biomedical and Health Sciences, Universidad Europea de Madrid, C\/Tajo s\/n, 28670 Villaviciosa de Od\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Bonet-Bo","sequence":"additional","affiliation":[{"name":"Embriovet SL, Pol\u00edgono Industrial de Piadela II-8, A Coru\u00f1a, 15300 Betanzos, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2287-3916","authenticated-orcid":false,"given":"Natividad","family":"P\u00e9rez-Villalobos","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, School of Biomedical and Health Sciences, Universidad Europea de Madrid, C\/Tajo s\/n, 28670 Villaviciosa de Od\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5669-6014","authenticated-orcid":false,"given":"Jose L.","family":"Pes\u00e1ntez-Pacheco","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural Sciences, School of Veterinary Medicine and Zootechnics, University of Cuenca, Av. Doce de Octubre, Cuenca 010220, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Luz","family":"P\u00e9rez-Solana","sequence":"additional","affiliation":[{"name":"Animal Reproduction Department, National Institute of Agronomic Research (INIA-CSIC), Puerta de Hierro Avenue s\/n, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0321-2431","authenticated-orcid":false,"given":"Eduardo","family":"de Mercado","sequence":"additional","affiliation":[{"name":"Animal Reproduction Department, National Institute of Agronomic Research (INIA-CSIC), Puerta de Hierro Avenue s\/n, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5301-2174","authenticated-orcid":false,"given":"Juan Carlos","family":"Gard\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, Veterinary and Experimental Sciences School, Catholic University of Valencia-San Vicente M\u00e1rtir, Guillem de Castro, 94, 46001 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arantxa","family":"Villagr\u00e1","sequence":"additional","affiliation":[{"name":"Institut Valenci\u00e0 d\u2019Investigacions Agr\u00e0ries (IVIA), CV-315, Km, 10700 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Sebasti\u00e1n","sequence":"additional","affiliation":[{"name":"Cowvet SL, Avda. Pa\u00eds Valenciano 6, Betera, 46117 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia Salom\u00e9","family":"P\u00e9rez-Garnelo","sequence":"additional","affiliation":[{"name":"Animal Reproduction Department, National Institute of Agronomic Research (INIA-CSIC), Puerta de Hierro Avenue s\/n, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2728-3464","authenticated-orcid":false,"given":"Daniel","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Embriovet SL, Pol\u00edgono Industrial de Piadela II-8, A Coru\u00f1a, 15300 Betanzos, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9718-0903","authenticated-orcid":false,"given":"Susana","family":"Astiz","sequence":"additional","affiliation":[{"name":"Animal Reproduction Department, National Institute of Agronomic Research (INIA-CSIC), Puerta de Hierro Avenue s\/n, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"366","DOI":"10.2527\/2001.792366x","article-title":"Inbreeding and effective population size of Japanese Black Cattle","volume":"79","author":"Nomura","year":"2001","journal-title":"J. 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