{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:11:29Z","timestamp":1790730689465,"version":"4.1.0"},"reference-count":53,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,7]],"date-time":"2022-02-07T00:00:00Z","timestamp":1644192000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2017R1E1A1A01073021"],"award-info":[{"award-number":["NRF-2017R1E1A1A01073021"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2020R1A5A2019210"],"award-info":[{"award-number":["2020R1A5A2019210"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Health and Welfare","award":["HI18C2392"],"award-info":[{"award-number":["HI18C2392"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Reactive oxygen species (ROS) generated by neutrophils provide a frontline defence against invading pathogens. We investigated the supportive effect of tonsil-derived mesenchymal stem cells (TMSCs) on ROS generation from neutrophils using promyelocytic HL-60 cells. Methods: Differentiated HL-60 (dHL-60) cells were cocultured with TMSCs isolated from 25 independent donors, and ROS generation in dHL-60 cells was measured using luminescence. RNA sequencing and real-time PCR were performed to identify the candidate genes of TMSCs involved in augmenting the oxidative burst of dHL-60 cells. Transcriptome analysis of TMSCs derived from 25 independent donors revealed high levels of procollagen C-endopeptidase enhancer 2 (PCOLCE2) in TMSCs, which were highly effective in potentiating ROS generation in dHL-60 cells. In addition, PCOLCE2 knockdown in TMSCs abrogated TMSC-induced enhancement of ROS production in dHL-60 cells, indicating that TMSCs increased the oxidative burst in dHL-60 cells via PCOLCE2. Furthermore, the direct addition of recombinant PCOLCE2 protein increased ROS production in dHL-60 cells. These results suggest that PCOLCE2 secreted by TMSCs may be used as a therapeutic candidate to enhance host defences by increasing neutrophil oxidative bursts. PCOLCE2 levels in TMSCs could be used as a marker to select TMSCs exhibiting high efficacy for enhancing neutrophil oxidative bursts.<\/jats:p>","DOI":"10.3390\/biology11020255","type":"journal-article","created":{"date-parts":[[2022,2,7]],"date-time":"2022-02-07T08:38:48Z","timestamp":1644223128000},"page":"255","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Procollagen C-Endopeptidase Enhancer 2 Secreted by Tonsil-Derived Mesenchymal Stem Cells Increases the Oxidative Burst of Promyelocytic HL-60 Cells"],"prefix":"10.3390","volume":"11","author":[{"given":"Hee-Soo","family":"Yoon","sequence":"first","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul 07084, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee-Yeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul 07084, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Ah","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Ewha Womans University, Seoul 07084, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7804-9264","authenticated-orcid":false,"given":"Yu-Hee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Ewha Womans University, Seoul 07084, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So-Youn","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Ewha Womans University, Seoul 07084, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2239-0225","authenticated-orcid":false,"given":"Han-Su","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, College of Medicine, Ewha Womans University, Seoul 07985, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihee-Lee","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine, Ewha Womans University, Seoul 07084, Korea"},{"name":"Inflammation-Cancer Microenvironment Research Center, College of Medicine, Ewha Womans University, Seoul 07084, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Ha","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, College of Medicine, Ewha Womans University, Seoul 07084, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joo-Won","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul 07084, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"373","DOI":"10.3389\/fcimb.2017.00373","article-title":"Neutrophils to the ROScue: Mechanisms of NADPH Oxidase Activation and Bacterial Resistance","volume":"7","author":"Nguyen","year":"2017","journal-title":"Front. 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