{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T08:33:08Z","timestamp":1771230788775,"version":"3.50.1"},"reference-count":65,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T00:00:00Z","timestamp":1649289600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32100377"],"award-info":[{"award-number":["32100377"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31902212"],"award-info":[{"award-number":["31902212"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties","award":["2021C02072"],"award-info":[{"award-number":["2021C02072"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["CARS-18"],"award-info":[{"award-number":["CARS-18"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Seminal fluid proteins provide factors necessary for development, storage, and activation of sperm. Altered expression of seminal fluid proteins can lead to defect in male infertility. We investigated the function of seminal fluid protein Sfp62 in the model lepidopteran insect Bombyx mori using CRISPR\/Cas9 mediated mutagenesis. The knockout of BmSfp62 gene led to male sterility but has no effect on female fertility. The mutation did not affect growth and development of the silkworm of both sexes. Motility of sperm in male mutants was decreased and the mRNA expression levels of other genes encoding seminal fluid proteins were altered in BmSfp62 mutants compared to the wild-type controls. The male sterility caused by mutation of BmSfp62 was stably inherited. As the proteins encoded by Sfp62 genes are conserved among lepidopteran species, Sfp62 is a potential target for the biological management of lepidopteran pests.<\/jats:p>","DOI":"10.3390\/biology11040561","type":"journal-article","created":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T13:39:51Z","timestamp":1649338791000},"page":"561","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["CRISPR\/Cas9 Mediated Disruption of Seminal Fluid Protein Sfp62 Induces Male Sterility in Bombyx mori"],"prefix":"10.3390","volume":"11","author":[{"given":"Xia","family":"Xu","sequence":"first","affiliation":[{"name":"Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jine","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Du","sequence":"additional","affiliation":[{"name":"Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lusong","family":"Yao","sequence":"additional","affiliation":[{"name":"Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Davidovics, R., Saw, Y.L., Brown, C.O., Prinz, M., McKiernan, H.E., Danielson, P.B., and Legg, K.M. (2022). High-throughput seminal fluid identification by automated immunoaffinity mass spectrometry. J. Forensic. Sci., 1\u20137.","DOI":"10.1111\/1556-4029.14975"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1002\/mrd.22130","article-title":"Identification and function of proteolysis regulators in seminal fluid","volume":"80","author":"Laflamme","year":"2013","journal-title":"Mol. Reprod. Dev."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Findlay, G.D., Sitnik, J.L., Wang, W., Aquadro, C.F., Clark, N.L., and Wolfner, M.F. (2014). Evolutionary rate covariation identifies new members of a protein network required for Drosophila melanogaster female post-mating responses. PLoS Genet., 10.","DOI":"10.1371\/journal.pgen.1004108"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ram, K.R., and Wolfner, M.F. (2007). Sustained post-mating response in Drosophila melanogaster requires multiple seminal fluid proteins. PLoS Genet., 3.","DOI":"10.1371\/journal.pgen.0030238"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1002\/bies.950170509","article-title":"Seminal plasmin","volume":"17","author":"Sitaram","year":"1995","journal-title":"Bioessays"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Amaro, I.A., Ahmed-Braimah, Y.H., League, G.P., Pitcher, S.A., Avila, F.W., Cruz, P.C., Harrington, L.C., and Wolfner, M.F. (2021). Seminal fluid proteins induce transcriptome changes in the Aedes aegypti female lower reproductive tract. BMC Genom., 22.","DOI":"10.1186\/s12864-021-08201-0"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"20200068","DOI":"10.1098\/rstb.2020.0068","article-title":"Seminal fluid and accessory male investment in sperm competition","volume":"375","author":"Ramm","year":"2020","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.ygcen.2019.01.011","article-title":"On the evolutionary origins of insect seminal fluid proteins","volume":"278","author":"Sirot","year":"2019","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"E36","DOI":"10.2527\/jas.2006-578","article-title":"Seminal fluid signaling in the female reproductive tract: Lessons from rodents and pigs","volume":"85","author":"Robertson","year":"2007","journal-title":"J. Anim. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.anireprosci.2018.04.061","article-title":"Seminal plasma proteomes and sperm fertility","volume":"194","author":"Druart","year":"2018","journal-title":"Anim. Reprod. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/j.eururo.2021.08.014","article-title":"European Association of Urology Guidelines on Male Sexual and Reproductive Health: 2021 Update on Male Infertility","volume":"80","author":"Minhas","year":"2021","journal-title":"Eur. Urol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1111\/1556-4029.14646","article-title":"Direct seminal fluid identification by protease-free high-resolution mass spectrometry","volume":"66","author":"Brown","year":"2021","journal-title":"J. Forensic. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1600-0897.2011.01033.x","article-title":"Seminal plasma proteins: What role do they play?","volume":"66","author":"Kvist","year":"2011","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1530\/REP-09-0134","article-title":"Ca2+-stores in sperm: Their identities and functions","volume":"138","author":"Costello","year":"2009","journal-title":"Reproduction"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: A new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic. Acids Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e53913","DOI":"10.7554\/eLife.53913","article-title":"TMEM95 is a sperm membrane protein essential for mammalian fertilization","volume":"9","author":"Hamze","year":"2020","journal-title":"Elife"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"11493","DOI":"10.1073\/pnas.1922650117","article-title":"Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice","volume":"117","author":"Noda","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1146\/annurev-cellbio-120219-021751","article-title":"The Fertilization Enigma: How Sperm and Egg Fuse","volume":"37","author":"Deneke","year":"2021","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1007\/BF00003405","article-title":"Proteins of seminal fluid and spermatozoa in the trout (Oncorhynchus mykiss): Partial characterization and variations","volume":"8","author":"Loir","year":"1990","journal-title":"Fish Physiol. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cca.2004.08.016","article-title":"Estrogenic octylphenol affects seminal fluid production and its biochemical composition of eelpout (Zoarces viviparus)","volume":"139","author":"Rasmussen","year":"2004","journal-title":"Comp. Biochem. Physiol. Part C Toxicol. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2854","DOI":"10.1073\/pnas.052707599","article-title":"Binding of rainbow trout sperm to egg is mediated by strong carbohydrate-to-carbohydrate interaction between (KDN)GM3 (deaminated neuraminyl ganglioside) and Gg3-like epitope","volume":"99","author":"Yu","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1046\/j.1440-169x.1999.00463.x","article-title":"A high molecular weight glycoprotein in seminal plasma is a sperm immobilizing factor in the teleost Nile tilapia, Oreochromis niloticus","volume":"41","author":"Mochida","year":"1999","journal-title":"Dev. Growth Differ."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"806982","DOI":"10.3389\/fcell.2021.806982","article-title":"The Sperm Protein Spaca6 is Essential for Fertilization in Zebrafish","volume":"9","author":"Binner","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6704","DOI":"10.1111\/mec.14384","article-title":"Gene expression changes in male accessory glands during ageing are accompanied by reproductive decline in Drosophila melanogaster","volume":"26","author":"Koppik","year":"2017","journal-title":"Mol. Ecol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.cois.2016.03.002","article-title":"Mating induces developmental changes in the insect female reproductive tract","volume":"13","author":"Carmel","year":"2016","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/ede.12068","article-title":"Ontogenetic changes in seminal fluid gene expression and the protein composition of cricket seminal fluid","volume":"16","author":"Simmons","year":"2014","journal-title":"Evol. Dev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1111\/j.1420-9101.2009.01889.x","article-title":"Effect of male age on sperm traits and sperm competition success in the guppy (Poecilia reticulata)","volume":"23","author":"Gasparini","year":"2010","journal-title":"J. Evol. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1007\/s00265-009-0806-6","article-title":"Seminal fluid protein depletion and replenishment in the fruit fly, Drosophila melanogaster: An ELISA-based method for tracking individual ejaculates","volume":"63","author":"Sirot","year":"2009","journal-title":"Behav. Ecol. Sociobiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1146\/annurev-ento-120709-144823","article-title":"Insect seminal fluid proteins: Identification and function","volume":"56","author":"Avila","year":"2011","journal-title":"Annu. Rev. Entomol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1095\/biolreprod.107.062505","article-title":"Annexins are candidate oviductal receptors for bovine sperm surface proteins and thus may serve to hold bovine sperm in the oviductal reservoir","volume":"77","author":"Ignotz","year":"2007","journal-title":"Biol. Reprod."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"LaFlamme, B.A., Ram, K.R., and Wolfner, M.F. (2012). The Drosophila melanogaster seminal fluid protease \u201cseminase\u201d regulates proteolytic and post-mating reproductive processes. PLoS Genet., 8.","DOI":"10.1371\/journal.pgen.1002435"},{"key":"ref_32","first-page":"651","article-title":"What the study of seminal fluid proteins in Drosophila tells us about the evolution of reproduction","volume":"30","author":"Denis","year":"2014","journal-title":"Med. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1111\/jeb.13597","article-title":"Molecular evolution of the sex peptide network in Drosophila","volume":"33","author":"McGeary","year":"2020","journal-title":"J. Evol. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1007\/s00018-003-3052","article-title":"Sex-peptides: Seminal peptides of the Drosophila male","volume":"60","author":"Kubli","year":"2003","journal-title":"Cell Mol. Life Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1002\/mrd.22980","article-title":"Culex pipiens sperm motility is initiated by a trypsin-like protease from male accessory glands","volume":"85","author":"Stephens","year":"2018","journal-title":"Mol. Reprod. Dev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1126\/science.aan5478","article-title":"Driving mosquito refractoriness to Plasmodium falciparum with engineered symbiotic bacteria","volume":"357","author":"Wang","year":"2017","journal-title":"Science"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Scolari, F., Gomulski, L.M., Ribeiro, J.M., Siciliano, P., Meraldi, A., Falchetto, M., Bonomi, A., Manni, M., Gabrieli, P., and Malovini, A. (2012). Transcriptional profiles of mating-responsive genes from testes and male accessory glands of the Mediterranean fruit fly, Ceratitis capitata. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0046812"},{"key":"ref_38","first-page":"20171486","article-title":"Socially cued seminal fluid gene expression mediates responses in ejaculate quality to sperm competition risk","volume":"284","author":"Simmons","year":"2017","journal-title":"Proc. Biol. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"103243","DOI":"10.1016\/j.ibmb.2019.103243","article-title":"Mutation of the seminal protease gene, serine protease 2, results in male sterility in diverse lepidopterans","volume":"116","author":"Xu","year":"2020","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1093\/infdis\/jiab049","article-title":"Novel Sterile Insect Technology Program Results in Suppression of a Field Mosquito Population and Subsequently to Reduced Incidence of Dengue","volume":"224","author":"Rebechi","year":"2021","journal-title":"J. Infect. Dis."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Marec, F., and Vreysen, M.J.B. (2019). Advances and Challenges of Using the Sterile Insect Technique for the Management of Pest Lepidoptera. Insects, 10.","DOI":"10.3390\/insects10110371"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"426","DOI":"10.4103\/1008-682X.175781","article-title":"Seminal biomarkers for the evaluation of male infertility","volume":"18","author":"Bieniek","year":"2016","journal-title":"Asian J. Androl."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1093\/jme\/tjab024","article-title":"Sterile Insect Technique: Lessons From the Past","volume":"58","author":"Benedict","year":"2021","journal-title":"J. Med. Entomol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.cell.2016.10.044","article-title":"CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes","volume":"168","author":"Komor","year":"2017","journal-title":"Cell"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/jisesa\/iew010","article-title":"Evidence of Male Hair Pencil Pheromone in Choristoneura fumiferana (Lepidoptera: Tortricidae)","volume":"16","author":"Roscoe","year":"2016","journal-title":"J. Insect Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"11751","DOI":"10.1073\/pnas.0500954102","article-title":"Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase","volume":"102","author":"Tan","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_47","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Zuckerkandl, E. (1965). Evolutionary divergence and convergence in proteins. Evolving Genes & Proteins, Academic Press.","DOI":"10.1016\/B978-1-4832-2734-4.50017-6"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/nbt.2501","article-title":"Efficient genome editing in zebrafish using a CRISPR-Cas system","volume":"31","author":"Hwang","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1258096","DOI":"10.1126\/science.1258096","article-title":"Genome editing. The new frontier of genome engineering with CRISPR-Cas9","volume":"346","author":"Doudna","year":"2014","journal-title":"Science"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1016\/j.cell.2014.05.010","article-title":"Development and applications of CRISPR-Cas9 for genome engineering","volume":"157","author":"Hsu","year":"2014","journal-title":"Cell"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1111\/1744-7917.12657","article-title":"Identification of a germline-expression promoter for genome editing in Bombyx mori","volume":"26","author":"Xu","year":"2018","journal-title":"Insect Sci."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Rodriguez-Martinez, H., Martinez, E.A., Calvete, J.J., Pena Vega, F.J., and Roca, J. (2021). Seminal Plasma: Relevant for Fertility?. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22094368"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5186","DOI":"10.1111\/febs.13110","article-title":"Genome modification by CRISPR\/Cas9","volume":"281","author":"Ma","year":"2014","journal-title":"FEBS J."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/j.tplants.2019.09.006","article-title":"CRISPR\/Cas System: Recent Advances and Future Prospects for Genome Editing","volume":"24","author":"Manghwar","year":"2019","journal-title":"Trends. Plant. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1016\/j.cub.2004.08.028","article-title":"Genes regulated by mating, sperm, or seminal proteins in mated female Drosophila melanogaster","volume":"14","author":"McGraw","year":"2004","journal-title":"Curr. Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"36647","DOI":"10.1074\/jbc.M103885200","article-title":"AMY-1, a c-Myc-binding protein, is localized in the mitochondria of sperm by association with S-AKAP84, an anchor protein of cAMP-dependent protein kinase","volume":"276","author":"Furusawa","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1534\/genetics.108.086934","article-title":"Post-mating gene expression profiles of female Drosophila melanogaster in response to time and to four male accessory gland proteins","volume":"179","author":"McGraw","year":"2008","journal-title":"Genetics"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Chakravorty, S., Vu, H., Foelber, V., and Vigoreaux, J.O. (2014). Mutations of the Drosophila myosin regulatory light chain affect courtship song and reduce reproductive success. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0090077"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1534\/genetics.115.181644","article-title":"The Female Post-Mating Response Requires Genes Expressed in the Secondary Cells of the Male Accessory Gland in Drosophila melanogaster","volume":"202","author":"Sitnik","year":"2016","journal-title":"Genetics"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1111\/imb.12297","article-title":"Tissue-specific expression and silencing phenotypes of mitochondrial phosphate carrier paralogues in several insect species","volume":"26","author":"Sugahara","year":"2017","journal-title":"Insect Mol. Biol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.theriogenology.2020.08.028","article-title":"Overexpression of regucalcin mitigates the ageing-related changes in oxidative stress and sperm quality","volume":"157","author":"Silva","year":"2020","journal-title":"Theriogenology"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1126\/science.1072029","article-title":"Penetration, adhesion, and fusion in mammalian sperm-egg interaction","volume":"296","author":"Primakoff","year":"2002","journal-title":"Science"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"729440","DOI":"10.3389\/fphys.2021.729440","article-title":"Beyond Sperm and Male Accessory Gland Proteins: Exploring Insect Reproductive Metabolomes","volume":"12","author":"Scolari","year":"2021","journal-title":"Front. Physiol."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/11\/4\/561\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:49:57Z","timestamp":1760136597000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/11\/4\/561"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,7]]},"references-count":65,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["biology11040561"],"URL":"https:\/\/doi.org\/10.3390\/biology11040561","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,7]]}}}