{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T04:12:39Z","timestamp":1780805559023,"version":"3.54.1"},"reference-count":72,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Foundation for Science and Technology","award":["UIDB\/04326\/2020"],"award-info":[{"award-number":["UIDB\/04326\/2020"]}]},{"name":"FCT\u2014Foundation for Science and Technology","award":["LA\/P\/0101\/2020"],"award-info":[{"award-number":["LA\/P\/0101\/2020"]}]},{"name":"FCT\u2014Foundation for Science and Technology","award":["P33927"],"award-info":[{"award-number":["P33927"]}]},{"name":"FCT\u2014Foundation for Science and Technology","award":["P33089"],"award-info":[{"award-number":["P33089"]}]},{"name":"Austrian Science Fund (FWF)","award":["UIDB\/04326\/2020"],"award-info":[{"award-number":["UIDB\/04326\/2020"]}]},{"name":"Austrian Science Fund (FWF)","award":["LA\/P\/0101\/2020"],"award-info":[{"award-number":["LA\/P\/0101\/2020"]}]},{"name":"Austrian Science Fund (FWF)","award":["P33927"],"award-info":[{"award-number":["P33927"]}]},{"name":"Austrian Science Fund (FWF)","award":["P33089"],"award-info":[{"award-number":["P33089"]}]},{"name":"University of Vienna","award":["UIDB\/04326\/2020"],"award-info":[{"award-number":["UIDB\/04326\/2020"]}]},{"name":"University of Vienna","award":["LA\/P\/0101\/2020"],"award-info":[{"award-number":["LA\/P\/0101\/2020"]}]},{"name":"University of Vienna","award":["P33927"],"award-info":[{"award-number":["P33927"]}]},{"name":"University of Vienna","award":["P33089"],"award-info":[{"award-number":["P33089"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>The increase in bacterial resistance to antibiotics has led researchers to find new compounds or find combinations between different compounds with potential antibacterial action and with the ability to prevent the development of antibiotic resistance. Polyoxotungstates (POTs) are inorganic clusters that may fulfill that need, either individually or in combination with antibiotics. Herein, we report the ability of the polyoxotungstates (POTs) with Wells-Dawson P2W18, P2W17, P2W15, and Preyssler P5W30 type structures to differently affect Gram-negative and Gram-positive microorganisms, either susceptible or resistant to antibiotics. The compound P5W30 showed the highest activity against the majority of the tested bacterial strains in comparison with the other tested POTs (P2W15, P2W17 and P2W18) that did not show inhibition zones for the Gram-negative bacteria, A. baumanii I73775, E. coli DSM 1077, E. coli I73194, K. pneumoniae I7092374, and P. aeruginosa C46281). Generally, the results evidenced that Gram-positive bacteria are more susceptible to the POTs tested. The compound P5W30 was the one most active against S. aureus ATCC 6538 and MRSA16, reaching &lt;0.83 mg\u00b7mL\u22121 (100 \u03bcM) and 4.96 mg\u00b7mL\u22121 (600 \u03bcM), respectively. Moreover, it was verified by NMR spectroscopy that the most promising POT, P5W30, remains intact under all the experimental conditions, after 24 h at 37 \u00b0C. This prompted us to further evaluate the anti-quorum sensing activity of P5W30 using the biosensor Chromobacterium violaceum CV026, as well as its antibiofilm activity both individually and in combination with the antibiotic cefoxitin against the methicillin-resistant Staphylococcus aureus 16 (MRSA16). P5W30 showed a synergistic antibacterial effect with the antibiotic cefoxitin and chloramphenicol against MRSA16. Moreover, the antibiofilm activity of P5W30 was more pronounced when used individually, in comparison with the combination with the antibiotic cefoxitin. Finally, the antiviral activity of P5W30 was tested using the coliphage Q\u03b2, showing a dose-dependent response. The maximum inactivation was observed at 750 \u03bcM (6.23 mg\u00b7mL\u22121). In sum, P5W30 shows anti-quorum sensing and antibiofilm activities besides being a potent antibacterial agent against S. aureus and to exhibit antiviral activities against enteric viruses.<\/jats:p>","DOI":"10.3390\/biology11070994","type":"journal-article","created":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T20:53:02Z","timestamp":1656622382000},"page":"994","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3878-6948","authenticated-orcid":false,"given":"Leonor","family":"Faleiro","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"Algarve Biomedical Center\u2014Research Institute, 8005-139 Faro, Portugal"},{"name":"Champalimaud Research Program, Champalimaud Centre for the Unknown, 1400-038 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Marques","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"Algarve Biomedical Center\u2014Research Institute, 8005-139 Faro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o","family":"Martins","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"Centro de Ci\u00eancias do Mar (CCMar), Universidade do Algarve, 8005-139 Faro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5608-021X","authenticated-orcid":false,"given":"Lu\u00edsa","family":"Jord\u00e3o","sequence":"additional","affiliation":[{"name":"Departamento de Sa\u00fade Ambiental (DSA), Instituto Nacional de Sa\u00fade Doutor Ricardo Jorge (INSA), Unidade de Investiga\u00e7\u00e3o e Desenvolvimento, 1649-016 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6138-1172","authenticated-orcid":false,"given":"Isabel","family":"Nogueira","sequence":"additional","affiliation":[{"name":"MicroLab, Instituto Superior T\u00e9cnico, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4375-0103","authenticated-orcid":false,"given":"Nadiia I.","family":"Gumerova","sequence":"additional","affiliation":[{"name":"Universit\u00e4t Wien, Fakult\u00e4t f\u00fcr Chemie, Institut f\u00fcr Biophysikalische Chemie, 1090 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5919-0553","authenticated-orcid":false,"given":"Annette","family":"Rompel","sequence":"additional","affiliation":[{"name":"Universit\u00e4t Wien, Fakult\u00e4t f\u00fcr Chemie, Institut f\u00fcr Biophysikalische Chemie, 1090 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4858-3201","authenticated-orcid":false,"given":"Manuel","family":"Aureliano","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"},{"name":"Centro de Ci\u00eancias do Mar (CCMar), Universidade do Algarve, 8005-139 Faro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,30]]},"reference":[{"key":"ref_1","first-page":"9","article-title":"Origins and evolution of antibiotic resistance","volume":"12","author":"Davies","year":"1996","journal-title":"Microbiologia"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mancuso, G., Midiri, A., Gerace, E., and Biondo, C. 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