{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:44:52Z","timestamp":1790034292354,"version":"4.0.1"},"reference-count":40,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T00:00:00Z","timestamp":1657065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Alcoholic liver disease is associated with the production of highly reactive free radicals by ethanol and its metabolites. Free radicals not only induce liver oxidation and damage tissues, but also stimulate an inflammatory response in hepatocytes, leading to severe liver disease. In order to improve alcoholic liver disease, enzymatic porcine placenta hydrolysate was studied by exploring various materials. Enzymatic porcine placenta hydrolysate (EPPH) contains various amino acids, peptides, and proteins, and is used as a useful substance in the body. In this study, changes were confirmed in indicators related to the antioxidant efficacy of EPPH in vitro and in vivo. EPPH inhibits an EtOH-induced decrease in superoxide dismutase and catalase activity through inhibition of free radicals without endogenous cytotoxicity. EPPH has been observed to have a partial effect on common liver function factors such as liver weight, ALT, AST, ALP, and GGT. In addition, EPPH affected changes in fat regulators and inflammatory cytokines in blood biochemical assays. It was confirmed that EPPH was involved in fat metabolism in hepatocytes by regulating PPAR\u03b1 in an alcoholic liver disease animal model. Therefore, EPPH strongly modulates Bcl-2 and BAX involved in apoptosis, thereby exhibiting cytochrome P450 (CYP)-inhibitory effects in alcoholic liver disease cells. As a result, this study confirmed that EPPH is a substance that can help liver health by improving liver disease in an alcoholic liver disease animal model.<\/jats:p>","DOI":"10.3390\/biology11071012","type":"journal-article","created":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T09:41:10Z","timestamp":1657100470000},"page":"1012","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Potential Effect of Enzymatic Porcine Placental Hydrolysate (EPPH) to Improve Alcoholic Liver Disease (ALD) by Promoting Lipolysis in the Liver"],"prefix":"10.3390","volume":"11","author":[{"given":"Hak Yong","family":"Lee","sequence":"first","affiliation":[{"name":"Invivo Co., Ltd., 121, Deahak-ro, Seongbuk-gu, Nonsan 32992, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Mi","family":"Park","sequence":"additional","affiliation":[{"name":"Invivo Co., Ltd., 121, Deahak-ro, Seongbuk-gu, Nonsan 32992, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Yeop","family":"Shin","sequence":"additional","affiliation":[{"name":"Invivo Co., Ltd., 121, Deahak-ro, Seongbuk-gu, Nonsan 32992, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8816-2976","authenticated-orcid":false,"given":"Kwang Hyun","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Oriental, Nambu University, 23 Chumdanjungang-ro, Gwangsan-gu, Gwangju 62271, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Ju","family":"Kim","sequence":"additional","affiliation":[{"name":"Ubio, Unimed Bldg. 69, Samjeon-ro, Songpa-gu, Seoul 05567, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Myung","family":"Yoon","sequence":"additional","affiliation":[{"name":"Ubio, Unimed Bldg. 69, Samjeon-ro, Songpa-gu, Seoul 05567, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keun Nam","family":"Kim","sequence":"additional","affiliation":[{"name":"Unimed Pharm Inc., Unimed Bldg. 69, Samjeon-ro, Songpa-gu, Seoul 05567, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Jeong","family":"Yang","sequence":"additional","affiliation":[{"name":"Korea Food Research Institute, 245, Nonsaengmyeong-ro, Wansan-gu, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Jung","family":"Kim","sequence":"additional","affiliation":[{"name":"Korea Food Research Institute, 245, Nonsaengmyeong-ro, Wansan-gu, Wanju 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo-Cheol","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Kunsan National University, 558 Daehak-ro, Miryong-dong, Gunsan 54150, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In-Ah","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Kunsan National University, 558 Daehak-ro, Miryong-dong, Gunsan 54150, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/j.freeradbiomed.2007.11.004","article-title":"CYP2E1 and oxidative liver injury by alcohol","volume":"44","author":"Lu","year":"2008","journal-title":"Free Radic. 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