{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T18:02:55Z","timestamp":1764784975155,"version":"build-2065373602"},"reference-count":23,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Health (\u201cMinistero della Salute\u201d)","award":["I34I19001520001"],"award-info":[{"award-number":["I34I19001520001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Carbonic anhydrase inhibitors (CAIs) are prescription drugs also used in doping to dilute urine samples and tamper with urinalyses. Dorzolamide, brinzolamide, and acetazolamide are prohibited by the World Anti-Doping Agency. Detecting CAIs and their metabolites in biological samples is crucial to documenting misuse in doping. We quantified dorzolamide, brinzolamide, acetazolamide, and their metabolites in the urine and hair of 88 patients under treatment for ocular hypertension or glaucoma. Samples of the patients\u2019 relatives were analyzed to assess potential for accidental exposure. After washing, 25 mg hair was incubated with an acidic buffer at 100 \u00b0C for 1 h. After cooling and centrifugation, the supernatant was analyzed by ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS\/MS). Urine (100 \u03bcL) was diluted and centrifuged before UHPLC-MS\/MS analysis. Run time was 8 min through a reverse-phase column with a mobile phase gradient. MS\/MS analysis was performed in a multiple-reaction monitoring mode after positive electrospray ionization. Median urinary concentration was 245 ng\/mL (IQR: 116.2\u2013501 ng\/mL) for dorzolamide, 81.1 ng\/mL (IQR: 35.9\u2013125.3 ng\/mL) for N-deethyl-dorzolamide, 0.77 ng\/mL (IQR: 0.64 ng\/mL\u20130.84 ng\/mL) for N-acetyl-dorzolamide, 38.9 ng\/mL (IQR: 20.4\u201379.2 ng\/mL) for brinzolamide, and 72.8 ng\/mL (IQR: 20.7\u2013437.3 ng\/mL) for acetazolamide. Median hair concentration was 0.48 ng\/mg (IQR: 0.1\u20130.98 ng\/mg) for dorzolamide, 0.07 ng\/mg (IQR: 0.06\u20130.08 ng\/mg) for N-deethyl-dorzolamide, 0.40 ng\/mL (IQR: 0.13\u20131.95 ng\/mL) for brinzolamide. Acetazolamide was detected in only one hair sample. Dorzolamide and brinzolamide were detected in the urine of three and one relatives, respectively. Cutoff concentrations of urinary dorzolamide and brinzolamide are necessary to preclude false positives due to contamination or passive exposure. We reported the first concentrations of brinzolamide in hair.<\/jats:p>","DOI":"10.3390\/biology11101379","type":"journal-article","created":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T21:22:23Z","timestamp":1663795343000},"page":"1379","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Quantification of Carbonic Anhydrase Inhibitors and Metabolites in Urine and Hair of Patients and Their Relatives"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0240-5277","authenticated-orcid":false,"given":"Alfredo Fabrizio","family":"Lo Faro","sequence":"first","affiliation":[{"name":"Unit of Forensic Toxicology, Section of Legal Medicine, Department of Biomedical Sciences and Public Health, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasio","family":"Tini","sequence":"additional","affiliation":[{"name":"Unit of Forensic Toxicology, Section of Legal Medicine, Department of Biomedical Sciences and Public Health, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Bambagiotti","sequence":"additional","affiliation":[{"name":"Unit of Forensic Toxicology, Section of Legal Medicine, Department of Biomedical Sciences and Public Health, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9218-7818","authenticated-orcid":false,"given":"Filippo","family":"Pirani","sequence":"additional","affiliation":[{"name":"Unit of Forensic Toxicology, Section of Legal Medicine, Department of Biomedical Sciences and Public Health, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Faragalli","sequence":"additional","affiliation":[{"name":"Center of Epidemiology Biostatistics and Information Technology, Department of Biomedical Sciences and Public Health, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavia","family":"Carle","sequence":"additional","affiliation":[{"name":"Center of Epidemiology Biostatistics and Information Technology, Department of Biomedical Sciences and Public Health, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Pacella","sequence":"additional","affiliation":[{"name":"Department of Sense Organs, Faculty of Medicine and Dentistry, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, RM, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Artan","family":"Ceka","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Reunited Hospitals Torrette of Ancona, Via Conca 71, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Moretti","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Reunited Hospitals Torrette of Ancona, Via Conca 71, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Gottardi","sequence":"additional","affiliation":[{"name":"Comedical srl, Via della Cooperazione 29, 38123 Trento, TN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1151-7887","authenticated-orcid":false,"given":"Nicola Vito","family":"Lassandro","sequence":"additional","affiliation":[{"name":"Eye Clinic, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Nicolai","sequence":"additional","affiliation":[{"name":"Eye Clinic, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Lupidi","sequence":"additional","affiliation":[{"name":"Eye Clinic, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cesare","family":"Mariotti","sequence":"additional","affiliation":[{"name":"Eye Clinic, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco Paolo","family":"Busard\u00f2","sequence":"additional","affiliation":[{"name":"Unit of Forensic Toxicology, Section of Legal Medicine, Department of Biomedical Sciences and Public Health, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3332-9518","authenticated-orcid":false,"given":"Jeremy","family":"Carlier","sequence":"additional","affiliation":[{"name":"Unit of Forensic Toxicology, Section of Legal Medicine, Department of Biomedical Sciences and Public Health, Marche Polytechnic University, Via Tronto 10\/a, 60126 Ancona, AN, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1152\/physrev.1967.47.4.595","article-title":"Carbonic Anhydrase: Chemistry, Physiology, and Inhibition","volume":"47","author":"Maren","year":"1967","journal-title":"Physiol. 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