{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T03:15:35Z","timestamp":1771125335991,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T00:00:00Z","timestamp":1669766400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National International Cooperation Program,","award":["2011DFA10101103"],"award-info":[{"award-number":["2011DFA10101103"]}]},{"name":"National International Cooperation Program,","award":["30871855"],"award-info":[{"award-number":["30871855"]}]},{"name":"National International Cooperation Program,","award":["2020YFH0020, 2021YFYZ0030"],"award-info":[{"award-number":["2020YFH0020, 2021YFYZ0030"]}]},{"name":"National International Cooperation 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pathogens is a matter of widespread concern due to abuse of antibiotics. In order to develop a safe and economical immunopotentiator to raise the immunity and antibacterial response as a replacement for antibiotics, a recombinant yeast co-expressing pig interleukin-17 (IL-17) and IL-22 was constructed and designated as Po1h-pINA1297-IL-17\/22. To evaluate the immunoregulator activities of Po1h-pINA1297-IL-17\/22, two experiment groups (oral inoculation with Po1h-pINA1297 or Po1h-pINA1297-IL-17\/22) and a negative control group (PBS) were set up using 4-week-old female BALB\/c mice (10\/group). The level of cytokines, including IL-2, IL-4, IL-10, and IFN-\u03b3, were detected by ELISA, and the circulating CD4+ and CD8+ lymphocytes were quantified by flow cytometry. The IgG and secretory IgA (SIgA) levels in both small intestine and fecal matter were also measured by ELISA. The results indicated that the IgG antibody titer and SIgA concentration increased significantly in the Po1h-pINA1297-IL17\/22 group in comparison with the controls (p &lt; 0.05) and so did the cytokine levels in the serum (IL-2, IL-4, IL-10, and IFN-\u03b3). In addition, CD4+ and CD8+ T cells were also obviously elevated in the Po1h-pINA1297-IL17\/22 group on 35th day (p &lt; 0.05). After challenge with pathogenic Salmonella typhimurium, the Po1h-pINA1297-IL17\/22 group showed a relatively higher survival rate without obvious infectious symptoms. On the contrary, the mortality of control group reached 80% due to bacterial infection. As for the piglet experiment, 30 healthy 7-day piglets were similarly attributed into three groups. The oral inoculation of piglets with Po1h-pINA1297-IL17\/22 also markedly improved the growth performance and systemic immunity (up-regulations of IL-4, IL-6, IL-15, IL-17, IL-22, and IL-23). Overall, the results indicated that Po1h-pINA1297-IL17\/22 effectively promoted the humoral and cellular immunity against bacterial infection. These proved the promising potential of Po1h-pINA1297-IL-17\/22 to be a potent immunopotentiator for the prevention of microbial pathogen infections.<\/jats:p>","DOI":"10.3390\/biology11121747","type":"journal-article","created":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T05:45:22Z","timestamp":1669787122000},"page":"1747","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Porcine Interleukin-17 and 22 Co-Expressed by Yarrowia\u00a0lipolytica Enhance Immunity and Increase Protection against Bacterial Challenge in Mice and Piglets"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7303-2562","authenticated-orcid":false,"given":"Junjie","family":"Peng","sequence":"first","affiliation":[{"name":"College of Life Science, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Yang","sequence":"additional","affiliation":[{"name":"New Drug Pre-Production Key Laboratory of Sichuan Province, Chengdu 610093, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianlin","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine, Collaborative Innovation Center of Sichuan for Elderly Care and Health, Chengdu Medical College, Chengdu 610500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaohua","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Life Science, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5172-2247","authenticated-orcid":false,"given":"Linhan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinghao","family":"Deng","sequence":"additional","affiliation":[{"name":"College of Life Science, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiangling","family":"Li","sequence":"additional","affiliation":[{"name":"Sichuan Animal Science Academy, Chengdu 610066, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuebin","family":"Lv","sequence":"additional","affiliation":[{"name":"Sichuan Animal Science Academy, Chengdu 610066, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Life Science, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,30]]},"reference":[{"key":"ref_1","first-page":"100030","article-title":"Antibiotic resistance in agriculture: Perspectives on upcoming strategies to overcome upsurge in resistance","volume":"2","author":"Mann","year":"2021","journal-title":"Curr. 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