{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T06:00:02Z","timestamp":1763445602452,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,11]],"date-time":"2023-02-11T00:00:00Z","timestamp":1676073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Federal Academic Leadership Program Priority 2030"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>We have studied the effect of two glucocorticoid hormones: hydrocortisone and its synthetic analogue methylprednisolone on the regeneration activity of head and tail blastema of the Girardia tigrina planarian. The regeneration activity was studied in head and tail blastema formed after resection by means of lifetime computer morphometry and immunohistochemical labeling of neoblasts. The search for orthologous proteins\u2014glucocorticoid receptors (hydrocortisone) was performed using the SmedGD database of the Schmidtea mediterranea planarian. The results indicate that both hormones influence the recovery rate of the regenerating head and tail blastema. The worms with regenerating tail blastema have less sensitivity to the hormones\u2019 treatment compared to the ones with regenerating head blastema. Hydrocortisone at a high concentration (10\u22123 M) suppressed the regeneration rate, while stimulating it at lower concentrations (10\u22124\u201310\u22126 M). The same concentrations of methylprednisolone inhibited the regeneration of head blastema, but did not affect the tail blastema regeneration. The two hormones acted differently: while hydrocortisone stimulated the proliferation of neoblasts in the periwound region, methylprednisolone reduced the mitotic activity, mainly on the tail zone furthest from the wound surface. We suggest that exogenous glucocorticoids can influence endogenous mechanisms of hormone-dependent regeneration.<\/jats:p>","DOI":"10.3390\/biology12020292","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T02:42:33Z","timestamp":1676256153000},"page":"292","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Morphogenic Effect of Exogenous Glucocorticoid Hormones in the Girardia tigrina Planarian (Turbellaria, Tricladida)"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7810-0675","authenticated-orcid":false,"given":"Artem","family":"Ermakov","sequence":"first","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Kudykina","sequence":"additional","affiliation":[{"name":"Institute of Medicine and Living System, Immanuel Kant Baltic Federal University, 236041 Kaliningrad, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arina","family":"Bykova","sequence":"additional","affiliation":[{"name":"Institute of Medicine and Living System, Immanuel Kant Baltic Federal University, 236041 Kaliningrad, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulyana","family":"Tkacheva","sequence":"additional","affiliation":[{"name":"Institute of Medicine and Living System, Immanuel Kant Baltic Federal University, 236041 Kaliningrad, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.gde.2016.06.007","article-title":"(Neo)blast from the past: New insights into planarian stem cell lineages","volume":"40","author":"Zhu","year":"2016","journal-title":"Curr. 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