{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:21:47Z","timestamp":1787588507452,"version":"build-2736575974"},"reference-count":88,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T00:00:00Z","timestamp":1680134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"French Ministry for Research"},{"name":"Lille university"},{"name":"French Intensive Care Society (FICS)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Sepsis-induced myopathy is characterized by muscle fiber atrophy, mitochondrial dysfunction, and worsened outcomes. Whether whole-body energy deficit participates in the early alteration of skeletal muscle metabolism has never been investigated. Three groups were studied: \u201cSepsis\u201d mice, fed ad libitum with a spontaneous decrease in caloric intake (n = 17), and \u201cSham\u201d mice fed ad libitum (Sham fed (SF), n = 13) or subjected to pair-feeding (Sham pair fed (SPF), n = 12). Sepsis was induced by the intraperitoneal injection of cecal slurry in resuscitated C57BL6\/J mice. The feeding of the SPF mice was restricted according to the food intake of the Sepsis mice. Energy balance was evaluated by indirect calorimetry over 24 h. The tibialis anterior cross-sectional area (TA CSA), mitochondrial function (high-resolution respirometry), and mitochondrial quality control pathways (RTqPCR and Western blot) were assessed 24 h after sepsis induction. The energy balance was positive in the SF group and negative in both the SPF and Sepsis groups. The TA CSA did not differ between the SF and SPF groups, but was reduced by 17% in the Sepsis group compared with the SPF group (p &lt; 0.05). The complex-I-linked respiration in permeabilized soleus fibers was higher in the SPF group than the SF group (p &lt; 0.05) and lower in the Sepsis group than the SPF group (p &lt; 0.01). Pgc1\u03b1 protein expression increased 3.9-fold in the SPF mice compared with the SF mice (p &lt; 0.05) and remained unchanged in the Sepsis mice compared with the SPF mice; the Pgc1\u03b1 mRNA expression decreased in the Sepsis compared with the SPF mice (p &lt; 0.05). Thus, the sepsis-like energy deficit did not explain the early sepsis-induced muscle fiber atrophy and mitochondrial dysfunction, but led to specific metabolic adaptations not observed in sepsis.<\/jats:p>","DOI":"10.3390\/biology12040529","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T02:08:01Z","timestamp":1680228481000},"page":"529","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Sepsis-like Energy Deficit Is Not Sufficient to Induce Early Muscle Fiber Atrophy and Mitochondrial Dysfunction in a Murine Sepsis Model"],"prefix":"10.3390","volume":"12","author":[{"given":"Alexandre","family":"Pierre","sequence":"first","affiliation":[{"name":"Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1167-RID-AGE-Facteurs de Risque et D\u00e9terminants Mol\u00e9culaires des Maladies Li\u00e9es au Vieillissement, F-59000 Lille, France"},{"name":"Division of Intensive Care, H\u00f4pital Roger Salengro, CHU de Lille, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Bourel","sequence":"additional","affiliation":[{"name":"Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1167-RID-AGE-Facteurs de Risque et D\u00e9terminants Mol\u00e9culaires des Maladies Li\u00e9es au Vieillissement, F-59000 Lille, France"},{"name":"Division of Intensive Care, H\u00f4pital Roger Salengro, CHU de Lille, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael","family":"Favory","sequence":"additional","affiliation":[{"name":"Univ. 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Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1167-RID-AGE-Facteurs de Risque et D\u00e9terminants Mol\u00e9culaires des Maladies Li\u00e9es au Vieillissement, F-59000 Lille, France"},{"name":"Division of Nephrology, CHU de Lille, Universit\u00e9 de Lille, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7544-463X","authenticated-orcid":false,"given":"Bruno","family":"Bastide","sequence":"additional","affiliation":[{"name":"Univ. Lille, Univ. Artois, Univ. Littoral C\u00f4te d\u2019Opale, ULR 7369-URePSSS-Unit\u00e9 de Recherche Pluridisciplinaire Sport Sant\u00e9 Soci\u00e9t\u00e9, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Amouyel","sequence":"additional","affiliation":[{"name":"Univ. 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