{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T11:05:37Z","timestamp":1794049537110,"version":"4.8.0"},"reference-count":102,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T00:00:00Z","timestamp":1686787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bayer\u00aeAG (Leverkusen, Germany)","award":["491094227"],"award-info":[{"award-number":["491094227"]}]},{"name":"Deutsche Forschungsgemeinschaft","award":["491094227"],"award-info":[{"award-number":["491094227"]}]},{"name":"University of Veterinary Medicine Hannover","award":["491094227"],"award-info":[{"award-number":["491094227"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>In pregnant bitches, the response to oxytocin and denaverine hydrochloride in dystocia management is usually poor. To better understand the effect of both drugs on myometrial contractility, the circular and longitudinal muscle layers were examined in an organ bath. For each layer, three myometrial strips were stimulated twice, each with one of three oxytocin concentrations. The effect of denaverine hydrochloride was studied once in direct combination with oxytocin and alone with subsequent oxytocin administration. Contractions were recorded and evaluated for average amplitude, mean force, area under the curve (AUC), and frequency. Effects of different treatments were analyzed and compared within and between layers. In the circular layer, oxytocin significantly increased amplitude and mean force compared to untreated controls regardless of stimulation cycles or concentrations. In both layers, high oxytocin concentrations caused tonic contractions, while the lowest concentration created regular rhythmic contractions. Longitudinal layer tissue responded to oxytocin with a significantly decreased contractility when stimulated twice, presumably a sign of desensitization. Denaverine hydrochloride neither affected oxytocin induced contractions nor showed a priming effect to subsequent oxytocin. Thus, no benefit of denaverine hydrochloride on myometrial contractility was found in the organ bath. Our results suggest a better efficiency of low-dose oxytocin in canine dystocia management.<\/jats:p>","DOI":"10.3390\/biology12060860","type":"journal-article","created":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T02:28:56Z","timestamp":1686796136000},"page":"860","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The In Vitro Contractile Response of Canine Pregnant Myometrium to Oxytocin and Denaverine Hydrochloride"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8143-2906","authenticated-orcid":false,"given":"Carolin","family":"Jungmann","sequence":"first","affiliation":[{"name":"Reproductive Unit, Clinic for Small Animals, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie-Charlotte","family":"Pyzik","sequence":"additional","affiliation":[{"name":"Tierklinik Neust\u00e4dter Bucht, 23730 Sierksdorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6694-3851","authenticated-orcid":false,"given":"Eva-Maria","family":"Packeiser","sequence":"additional","affiliation":[{"name":"Reproductive Unit, Clinic for Small Animals, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"K\u00f6rber","sequence":"additional","affiliation":[{"name":"Reproductive Unit, Clinic for Small Animals, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Hoppe","sequence":"additional","affiliation":[{"name":"Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, 30173 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7803-3556","authenticated-orcid":false,"given":"Gemma","family":"Mazzuoli-Weber","sequence":"additional","affiliation":[{"name":"Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, 30173 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4327-9780","authenticated-orcid":false,"given":"Sandra","family":"Goericke-Pesch","sequence":"additional","affiliation":[{"name":"Reproductive Unit, Clinic for Small Animals, University of Veterinary Medicine Hannover, 30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,15]]},"reference":[{"key":"ref_1","first-page":"3","article-title":"Biology and endocrinology of ovulation, pregnancy and parturition in the dog","volume":"39","author":"Concannon","year":"1989","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1530\/jrf.0.0960837","article-title":"Ovarian and pituitary function in dogs after hysterectomy","volume":"96","author":"Hoffmann","year":"1992","journal-title":"J. Reprod. Fertil."},{"key":"ref_3","first-page":"542","article-title":"Investigation of the endocrine control of parturition in the dog by application of an antigestagen","volume":"47","author":"Nohr","year":"1993","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1530\/REP-09-0140","article-title":"Canine placenta: A source of prepartal prostaglandins during normal and antiprogestin-induced parturition","volume":"139","author":"Kowalewski","year":"2010","journal-title":"Reproduction"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1530\/jrf.0.0840071","article-title":"Elevated concentrations of 13,14-dihydro-15-keto-prostaglandin F-2 in maternal plasma during prepartum luteolysis and parturition in dogs (Canis familiaris)","volume":"84","author":"Concannon","year":"1988","journal-title":"Reproduction"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.theriogenology.2020.01.082","article-title":"Canine conceptus-maternal communication during maintenance and termination of pregnancy, including the role of species-specific decidualization","volume":"150","author":"Kowalewski","year":"2020","journal-title":"Theriogenology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1055\/s-0029-1211280","article-title":"Investigations on hormonal changes around parturition in the dog and the occurrence of pregnancy-specific non conjugated oestrogens","volume":"102","author":"Hoffmann","year":"1994","journal-title":"Exp. Clin. Endocrinol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Zatta, S., Rehrauer, H., Gram, A., Boos, A., and Kowalewski, M.P. (2017). Transcriptome analysis reveals differences in mechanisms regulating cessation of luteal function in pregnant and non-pregnant dogs. BMC Genomics, 18.","DOI":"10.1186\/s12864-017-4084-9"},{"key":"ref_9","unstructured":"Kowalewski, M.P. (2018). Encyclopedia of Reproduction, Elsevier."},{"key":"ref_10","first-page":"211","article-title":"Physiological aspects of pregnancy and parturition in dogs","volume":"39","author":"Taverne","year":"1989","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/rda.12295","article-title":"Uterine and placental expression of canine oxytocin receptor during pregnancy and normal and induced parturition","volume":"49","author":"Gram","year":"2014","journal-title":"Reprod. Domest. Anim."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1590\/1414-431x20143969","article-title":"Gene expression of estrogen and oxytocin receptors in the uterus of pregnant and parturient bitches","volume":"48","author":"Veiga","year":"2015","journal-title":"Braz. J. Med. Biol. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/rda.12015","article-title":"Progesterone (PR), Oestrogen (ER-\u03b1 and ER-\u03b2) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches","volume":"47","author":"Derussi","year":"2012","journal-title":"Reprod. Domest. Anim."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1111\/j.1748-5827.1994.tb03863.x","article-title":"Dystocia in the bitch: A retrospective study of 182 cases","volume":"35","author":"Darvelid","year":"1994","journal-title":"J. Small Anim. Pract."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1751-0147-49-8","article-title":"A survey of dystocia in the Boxer breed","volume":"49","author":"Persson","year":"2007","journal-title":"Acta Vet. Scand."},{"key":"ref_16","first-page":"1527","article-title":"Abnormalities in pregnancy, parturition, and the periparturient period","volume":"Volume 5","author":"Ettinger","year":"2005","journal-title":"Textbook of Veterinary Internal Medicine"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"83","DOI":"10.5326\/JAAHA-MS-5122","article-title":"Primary Uterine Inertia in Four Labrador Bitches","volume":"47","author":"Davidson","year":"2011","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1016\/j.theriogenology.2010.01.006","article-title":"Hormonal concentrations in bitches with primary uterine inertia","volume":"73","author":"Fransson","year":"2010","journal-title":"Theriogenology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S0093-691X(97)00246-X","article-title":"Failure of luteolysis leads to prolonged gestation in a bitch: A case report","volume":"48","author":"Irons","year":"1997","journal-title":"Theriogenology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.2044-3862.2012.00241.x","article-title":"Single pup syndrome in an English Bulldog: Failure of luteolysis","volume":"17","author":"McLean","year":"2012","journal-title":"Companion Anim."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Jungmann, C., Gauguin Houghton, C., Goth Nielsen, F., Packeiser, E.-M., K\u00f6rber, H., Reichler, I.M., Balogh, O., and Goericke-Pesch, S. (2022). Involvement of Oxytocin and Progesterone Receptor Expression in the Etiology of Canine Uterine Inertia. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232113601"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.theriogenology.2021.09.003","article-title":"Investigations on the potential role of prostaglandin E2 in canine uterine inertia","volume":"175","author":"Rempel","year":"2021","journal-title":"Theriogenology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/s00441-021-03427-6","article-title":"Do uterine PTGS2, PGFS, and PTGFR expression play a role in canine uterine inertia?","volume":"385","author":"Rempel","year":"2021","journal-title":"Cell. Tissue Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.theriogenology.2020.06.033","article-title":"Uterine expression of smooth muscle alpha- and gamma-actin and smooth muscle myosin in bitches diagnosed with uterine inertia and obstructive dystocia","volume":"156","author":"Egloff","year":"2020","journal-title":"Theriogenology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e29846","DOI":"10.4161\/sgtp.29846","article-title":"Rho-associated coiled-coil containing kinases (ROCK)","volume":"5","author":"Julian","year":"2014","journal-title":"Small GTPases"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.ejcb.2013.09.002","article-title":"Physiological roles of Rho and Rho effectors in mammals","volume":"92","author":"Thumkeo","year":"2013","journal-title":"Eur. J. Cell. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1262\/jrd.2020-141","article-title":"Implications of the RhoA\/Rho associated kinase pathway and leptin in primary uterine inertia in the dog","volume":"67","author":"Frehner","year":"2021","journal-title":"J. Reprod. Dev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0195-5616(01)50207-7","article-title":"Uterine and fetal monitoring in the bitch","volume":"31","author":"Davidson","year":"2001","journal-title":"Vet. Clin. North Am. Small Anim. Pract."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/j.1748-5827.1974.tb05667.x","article-title":"Canine dystocia--a review of the literature","volume":"15","author":"Bennett","year":"1974","journal-title":"J. Small Anim. Pract."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"G\u00fcnzel-Apel, A.-R., and Bostedt, H. (2016). Reproduktionsmedizin und Neonatologie von Hund und Katze, Schattauer. [1st ed.].","DOI":"10.1055\/b-005-148982"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/j.1439-0531.2009.01405.x","article-title":"Dystocia in Numbers\u2014Evidence-Based Parameters for Intervention in the Dog: Causes for Dystocia and Treatment Recommendations","volume":"44","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.theriogenology.2008.04.038","article-title":"Endocrinology of pregnancy in the dog: A review","volume":"70","author":"Verstegen","year":"2008","journal-title":"Theriogenology"},{"key":"ref_33","first-page":"359","article-title":"A study of the motility of the intact uterus at term","volume":"73","author":"Ferguson","year":"1941","journal-title":"Surg. Gynecol. Obstet."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1016\/j.theriogenology.2009.12.008","article-title":"Calcium, parathyroid hormone, oxytocin and pH profiles in the whelping bitch","volume":"73","author":"Hollinshead","year":"2010","journal-title":"Theriogenology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1111\/j.1748-5827.2006.00071.x","article-title":"Primary uterine inertia in 27 bitches: Aetiology and treatment","volume":"47","author":"Fransson","year":"2006","journal-title":"J. Small Anim. Pract."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.theriogenology.2019.06.007","article-title":"Expression of uterine oxytocin receptors and blood progesterone, 13,14-dihydro-15-Keto-Prostaglandin F2\u03b1, and ionized calcium levels in dystocic bitches","volume":"135","author":"Tamminen","year":"2019","journal-title":"Theriogenology"},{"key":"ref_37","first-page":"115","article-title":"Pharmakologie und Klinik von O-(2-\u00c4thylbutoxy)-benzils\u00e4ure-2-dimethylamino\u00e4thylesterhydrochlorid (=Spasmalgan)","volume":"2","year":"1970","journal-title":"Zbl Pharm. 109"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1191\/096813098674115417","article-title":"The intracutaneous pain model in the assessment of analgesic efficacy","volume":"5","author":"Scharein","year":"1998","journal-title":"Pain. Rev."},{"key":"ref_39","first-page":"751","article-title":"Pharmakologische Vergleichsuntersuchungen mit Derivaten des a,a-Diphenyl-a-(2-\u00e4thylbutoxy)-essigs\u00e4ure-(b-dimethylaminoathyl) esters","volume":"22","year":"1969","journal-title":"Acta Biol. Med. Ger."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.rbmo.2011.03.026","article-title":"Myometrial response to neurotropic and musculotropic spasmolytic drugs in an extracorporeal perfusion model of swine uteri","volume":"23","author":"Geisler","year":"2011","journal-title":"Reprod. Biomed. Online"},{"key":"ref_41","unstructured":"Veyx, P.G. (2021, February 01). Senisblex\u2014Fachinformation. Available online: https:\/\/docplayer.org\/44440607-Sensiblex-tocospasmolytikum-geburtserleichterung-bessere-aufweitung-weniger-geburtsverletzungen-geringere-neugeborenenverluste-b-6.html."},{"key":"ref_42","unstructured":"Wollrab, J. (1975). Gutachten \u00fcber die vorklinische Pr\u00fcfung von Spasmalgan\u00ae bei Geburten von Rind, Schwein und Fleischfressern, Unpublished Work."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1080\/01652176.1994.9694467","article-title":"Aspects of obstetric care in the dog","volume":"16","author":"Taverne","year":"1994","journal-title":"Vet. Q."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Siena, G., and Milani, C. (2021). Usefulness of Maternal and Fetal Parameters for the Prediction of Parturition Date in Dogs. Animals, 11.","DOI":"10.3390\/ani11030878"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Elfers, K., Menne, L., Colnaghi, L., Hoppe, S., and Mazzuoli-Weber, G. (2023). Short- and Long-Term Effects of Cocaine on Enteric Neuronal Functions. Cells, 12.","DOI":"10.3390\/cells12040577"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Verhaar, N., Hoppe, S., Grages, A.M., Hansen, K., Neudeck, S., K\u00e4stner, S., and Mazzuoli-Weber, G. (2023). Dexmedetomidine Has Differential Effects on the Contractility of Equine Jejunal Smooth Muscle Layers in Vitro. Animals, 13.","DOI":"10.3390\/ani13061021"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1016\/j.theriogenology.2010.06.023","article-title":"In vitro comparison of myometrial contractility induced by aglepristone-oxytocin and aglepristone-PGF2alpha combinations at different stages of the estrus cycle in the bitch","volume":"74","author":"Gogny","year":"2010","journal-title":"Theriogenology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/978-1-0716-1759-5_2","article-title":"Human Myometrial Contractility Assays","volume":"2384","author":"Arrowsmith","year":"2022","journal-title":"Methods Mol. Biol."},{"key":"ref_49","first-page":"29","article-title":"Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development","volume":"2384","author":"Arrowsmith","year":"2018","journal-title":"J. Vis. Exp."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1097\/ALN.0000000000000940","article-title":"In Vitro Comparative Effect of Carbetocin and Oxytocin in Pregnant Human Myometrium with and without Oxytocin Pretreatment","volume":"124","author":"Cole","year":"2016","journal-title":"Anesthesiology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1213\/ANE.0000000000002834","article-title":"The Effect of Intermittent Oxytocin Pretreatment on Oxytocin-Induced Contractility of Human Myometrium in Vitro","volume":"128","author":"Talati","year":"2019","journal-title":"Anesth. Analg."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1213\/ANE.0000000000000682","article-title":"The Contractile Effects of Oxytocin, Ergonovine, and Carboprost and Their Combinations: An in Vitro Study on Human Myometrial Strips","volume":"120","author":"Balki","year":"2015","journal-title":"Anesth. Analg."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"75","DOI":"10.4149\/gpb_2016022","article-title":"The response of rat and human uterus to oxytocin from different gestational stages in vitro","volume":"36","author":"Alotaibi","year":"2017","journal-title":"Gen. Physiol. Biophys."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/0014-2999(96)00258-0","article-title":"Comparison of vasopressin and oxytocin receptors in the rat uterus and vascular tissue","volume":"308","author":"Anouar","year":"1996","journal-title":"Eur. J. Pharmacol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1644","DOI":"10.1016\/0002-9378(89)90942-3","article-title":"The antagonistic effect of oxytocin and relaxin on rat uterine segment contractility","volume":"161","author":"Goldsmith","year":"1989","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1111\/j.1476-5381.1966.tb01847.x","article-title":"Response of the mammalian uterus to prostaglandins under differing hormonal conditions","volume":"26","author":"Sullivan","year":"1966","journal-title":"Br. J. Pharmacol. Chemother."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/S0196-9781(01)00402-8","article-title":"Muscle layer- and region-dependent distributions of oxytocin receptors in the porcine myometrium","volume":"22","author":"Kitazawa","year":"2001","journal-title":"Peptides"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1111\/j.1476-5381.1983.tb10500.x","article-title":"Effects of ovarian steroids upon responses mediated by adrenoceptors in separated layers of the myometrium and in the costo-uterine muscle of the guinea-pig","volume":"79","author":"Hartley","year":"1983","journal-title":"Br. J. Pharmacol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1095\/biolreprod28.3.536","article-title":"Responses mediated by adrenoceptors in the separated layers of the myometrium and in the costo-uterine muscle of the guinea pig during the estrous cycle","volume":"28","author":"Hartley","year":"1983","journal-title":"Biol. Reprod."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1111\/j.1474-8673.1985.tb00556.x","article-title":"Uterine contractility and actions of catecholamines in longitudinal and circular uterine layers from ovariectomised guinea-pigs: The effects of ovarian steroids","volume":"5","author":"Adam","year":"1985","journal-title":"J. Auton. Pharmacol."},{"key":"ref_61","unstructured":"Eurell, J.A.C., Frappier, B.L., and Dellmann, H.-D. (2006). Dellmann\u2019s Textbook of Veterinary Histology, Blackwell Publishing. [6th ed.]."},{"key":"ref_62","first-page":"280","article-title":"Arrangement of Muscle Fibers in the Myometrium of the Human Uterus: A Mesoscopic Study","volume":"4","author":"Pino","year":"2017","journal-title":"MOJ Anat. Physiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1097\/ALN.0b013e318297d347","article-title":"Oxytocin Pretreatment Attenuates Oxytocin-induced Contractions in Human Myometrium in Vitro","volume":"119","author":"Balki","year":"2013","journal-title":"Anesthesiology"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1213\/ANE.0000000000001268","article-title":"The Recovery Time of Myometrial Responsiveness after Oxytocin-Induced Desensitization in Human Myometrium in Vitro","volume":"122","author":"Balki","year":"2016","journal-title":"Anesth. Analg."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.theriogenology.2005.05.048","article-title":"An in vitro study on spontaneous myometrial contractility in the mare during estrus and diestrus","volume":"65","author":"Hirsbrunner","year":"2006","journal-title":"Theriogenology"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.anireprosci.2017.04.001","article-title":"The myometrial contractility during late pregnancy in dairy cows, in vitro","volume":"181","author":"Ulbrich","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"ref_67","first-page":"436","article-title":"Control of myometrial contractility: Role and regulation of gap junctions","volume":"10","author":"Garfield","year":"1988","journal-title":"Oxf. Rev. Reprod. Biol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1126\/science.929182","article-title":"Gap junctions: Their presence and necessity in myometrium during parturition","volume":"198","author":"Garfield","year":"1977","journal-title":"Science"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1530\/REP-17-0761","article-title":"Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog","volume":"155","author":"Graubner","year":"2018","journal-title":"Reproduction"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1111\/j.1440-1681.1988.tb01124.x","article-title":"Comparison of the activity of circular and longitudinal myometrium from pregnant rats: Co-ordination between muscle layers","volume":"15","author":"Tomiyasu","year":"1988","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1111\/j.1476-5381.1986.tb16264.x","article-title":"The spasmogenic action of oxytocin in the rat uterus\u2014Comparison with other agonists","volume":"88","author":"Edwards","year":"1986","journal-title":"Br. J. Pharmacol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1177\/1933719115597788","article-title":"Differing in Vitro Potencies of Tocolytics and Progesterone in Myometrium from Singleton and Twin Pregnancies","volume":"23","author":"Arrowsmith","year":"2016","journal-title":"Reprod. Sci."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Turton, P., Arrowsmith, S., Prescott, J., Ballard, C., Bricker, L., Neilson, J., and Wray, S. (2013). A Comparison of the Contractile Properties of Myometrium from Singleton and Twin Pregnancies. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0063800"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"e781","DOI":"10.1016\/j.ajog.2016.08.015","article-title":"The combination tocolytic effect of magnesium sulfate and an oxytocin receptor antagonist in myometrium from singleton and twin pregnancies","volume":"215","author":"Arrowsmith","year":"2016","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_75","unstructured":"Gorriz Martin, L. (2013). An in Vitro Study on the Myometrial Contractility in Dairy Cattle before Calving and after Postpartum LPS Infusion. Relation to Blood Progesterone and Estradiol-17 [beta] Levels. [Ph.D. Thesis, Tier\u00e4rztliche Hochschule]."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0306-3623(98)00089-5","article-title":"Smooth muscle layer-specific variations in the autonomic innervation of bovine myometrium","volume":"32","author":"Taneike","year":"1999","journal-title":"Gen. Pharmacol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0014-2999(00)00026-1","article-title":"The mechanisms of alpha(2)-adrenoceptor agonist-induced contraction in longitudinal muscle of the porcine uterus","volume":"390","author":"Kitazawa","year":"2000","journal-title":"Eur. J. Pharmacol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1474-8673.1994.tb00603.x","article-title":"Muscle layer and regional differences in autonomic innervation and responsiveness to transmitter agents in swine myometrium","volume":"14","author":"Taneike","year":"1994","journal-title":"J. Auton. Pharmacol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1095\/biolreprod45.6.831","article-title":"Autonomic innervation of the circular and longitudinal layers in swine myometrium","volume":"45","author":"Taneike","year":"1991","journal-title":"Biol. Reprod."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/0014-2999(81)90497-0","article-title":"Effects of catecholamines on circular and longitudinal uterine muscle of the rat","volume":"76","author":"Chow","year":"1981","journal-title":"Eur. J. Pharmacol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1111\/j.1600-0773.1972.tb00685.x","article-title":"Effect of Noradrenaline and Isoprenaline on the Circular and Longitudinal Muscle of the Oestrogen Dominated Rabbit Uterus","volume":"31","author":"Nesheim","year":"1972","journal-title":"Acta Pharmacol. Toxicol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1007\/BF02954388","article-title":"Beitr\u00e4ge zur vergleichenden Anatomie und Entwickelungsgeschichte der Uterusmuskulatur","volume":"38","author":"Sobotta","year":"1891","journal-title":"Arch. F\u00fcr Mikrosk. Anat."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1139\/y87-124","article-title":"The sensitivity of the longitudinal and circular muscle layers of the rat\u2019s myometrium to oxytocin in vitro during pregnancy","volume":"65","author":"Crankshaw","year":"1987","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"e51","DOI":"10.1016\/j.ajog.2010.08.023","article-title":"Oxytocin exposure during labor among women with postpartum hemorrhage secondary to uterine atony","volume":"204","author":"Grotegut","year":"2011","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_85","first-page":"376","article-title":"Emergency hysterectomy for obstetric hemorrhage","volume":"64","author":"Clark","year":"1984","journal-title":"Obstet. Gynecol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1097\/01.AOG.0000191529.52596.c0","article-title":"Minimum oxytocin dose requirement after cesarean delivery for labor arrest","volume":"107","author":"Balki","year":"2006","journal-title":"Obstet. Gynecol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"S379","DOI":"10.1038\/sj.bjp.0707604","article-title":"Agonist-selective mechanisms of GPCR desensitization","volume":"153","author":"Kelly","year":"2008","journal-title":"Br. J. Pharmacol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1096\/fasebj.4.11.2165947","article-title":"Turning off the signal: Desensitization of beta-adrenergic receptor function","volume":"4","author":"Hausdorff","year":"1990","journal-title":"FASEB J."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"350","DOI":"10.2174\/1874467211205030004","article-title":"G-protein coupled receptor resensitization-appreciating the balancing act of receptor function","volume":"5","author":"Mohan","year":"2012","journal-title":"Curr. Mol. Pharmacol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"C413","DOI":"10.1152\/ajpcell.1995.268.2.C413","article-title":"Downregulation of the oxytocin receptor on cultured astroglial cells","volume":"268","author":"Strosser","year":"1995","journal-title":"Am. J. Physiol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1016\/0002-9378(94)90414-6","article-title":"Effect of oxytocin antagonists on the activation of human myometrium in vitro: Atosiban prevents oxytocin-induced desensitization","volume":"171","author":"Phaneuf","year":"1994","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cellsig.2017.01.024","article-title":"GPCR desensitization: Acute and prolonged phases","volume":"41","author":"Rajagopal","year":"2018","journal-title":"Cell. Signal."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1067\/mob.2003.22","article-title":"Oxytocin-induced desensitization of the oxytocin receptor","volume":"188","author":"Robinson","year":"2003","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1093\/humupd\/4.5.625","article-title":"Desensitization of oxytocin receptors in human myometrium","volume":"4","author":"Phaneuf","year":"1998","journal-title":"Hum. Reprod. Update"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1530\/jrf.0.1200091","article-title":"Loss of myometrial oxytocin receptors during oxytocin-induced and oxytocin-augmented labour","volume":"120","author":"Phaneuf","year":"2000","journal-title":"J. Reprod. Fertil."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1152\/ajplegacy.1963.204.4.732","article-title":"Behavior of uterine muscle in Na-deficient solutions; effects of oxytocin","volume":"204","author":"Marshall","year":"1963","journal-title":"Am. J. Physiol. Leg. Content"},{"key":"ref_97","unstructured":"Schr\u00f6der, M. (2008). Die Nichtinvasive Wehenmessung als Methode zur \u00dcberwachung Physiologischer und Pathologischer Geburtsvorg\u00e4nge bei der H\u00fcndin und der Welpenvitalit\u00e4t. [Ph.D. Thesis, Freie Universit\u00e4t Berlin]."},{"key":"ref_98","unstructured":"Golob, A.-L. (2015). Einsatz von Denaverinhydrochlorid bei Milchk\u00fchen und F\u00e4rsen mit Gest\u00f6rtem Geburtsvorgang. [Ph.D. Thesis, Universit\u00e4tsbibliothek der Justus-Liebig-Universit\u00e4t Gie\u00dfen]."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"5410","DOI":"10.3168\/jds.2018-14930","article-title":"Influence of denaverine hydrochloride on calving ease in Holstein-Friesian heifers","volume":"102","author":"Lange","year":"2019","journal-title":"J. Dairy. Sci."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"150","DOI":"10.15653\/TPG-170844","article-title":"Effect of denaverine hydrochloride application to heifers on the APGAR score and lactate concentration in newborn calves","volume":"46","author":"Lange","year":"2018","journal-title":"Tierarztl. Prax. Ausg. G Grosstiere Nutztiere"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"3570","DOI":"10.3168\/jds.2013-7535","article-title":"Denaverine hydrochloride and carbetocin increased welfare during and after parturition and enhanced subsequent fertility in cattle","volume":"97","author":"Zobel","year":"2014","journal-title":"J. Dairy. Sci."},{"key":"ref_102","first-page":"18","article-title":"Ergebnisse der Anwendung von Spasmotitrat\u00ae VEB Berlin-Chemie bei Geburten von Nutz- und Haustieren","volume":"26","author":"Wollrab","year":"1986","journal-title":"Medicam. Sonderausg. Veterin\u00e4rmedizin"}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/12\/6\/860\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:55:16Z","timestamp":1760126116000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/12\/6\/860"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,15]]},"references-count":102,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["biology12060860"],"URL":"https:\/\/doi.org\/10.3390\/biology12060860","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,15]]}}}