{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T18:28:52Z","timestamp":1778783332846,"version":"3.51.4"},"reference-count":64,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T00:00:00Z","timestamp":1695168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades (Spain)","award":["FPU19\/06126"],"award-info":[{"award-number":["FPU19\/06126"]}]},{"name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades (Spain)","award":["FI19\/00051 2019\/0172"],"award-info":[{"award-number":["FI19\/00051 2019\/0172"]}]},{"name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades (Spain)","award":["CIACIF\/2021\/016"],"award-info":[{"award-number":["CIACIF\/2021\/016"]}]},{"name":"Instituto de Salud Carlos III","award":["FPU19\/06126"],"award-info":[{"award-number":["FPU19\/06126"]}]},{"name":"Instituto de Salud Carlos III","award":["FI19\/00051 2019\/0172"],"award-info":[{"award-number":["FI19\/00051 2019\/0172"]}]},{"name":"Instituto de Salud Carlos III","award":["CIACIF\/2021\/016"],"award-info":[{"award-number":["CIACIF\/2021\/016"]}]},{"name":"Generalitat Valenciana (Spain)","award":["FPU19\/06126"],"award-info":[{"award-number":["FPU19\/06126"]}]},{"name":"Generalitat Valenciana (Spain)","award":["FI19\/00051 2019\/0172"],"award-info":[{"award-number":["FI19\/00051 2019\/0172"]}]},{"name":"Generalitat Valenciana (Spain)","award":["CIACIF\/2021\/016"],"award-info":[{"award-number":["CIACIF\/2021\/016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Background: In recent years, there has been an evident delay in childbearing and concerns have been raised about whether this increase in age affects reproductive outcomes. This study aimed to evaluate the effect of paternal age on obstetrical and perinatal outcomes in couples undergoing in vitro fertilization or intracytoplasmic sperm injection using autologous sperm and oocytes. Methods: This retrospective study evaluated obstetrical and perinatal outcomes from 14,125 couples that were arbitrarily divided into three groups according to paternal age at conception: \u226430 (n = 1164), 31\u201340 (n = 11,668) and &gt;40 (n = 1293). Statistics consisted of a descriptive analysis followed by univariate and multivariate models, using the youngest age group as a reference. Results: The study showed significantly longer pregnancies for the fathers aged 31\u201340 compared to \u226430 years. However, there were no significant differences for the type of delivery, gestational diabetes, anaemia, hypertension, delivery threat, premature rupture of membranes, preterm birth, very preterm birth, and the neonate\u2019s sex, weight, low birth weight, very low birth weight, length, cranial perimeter, Apgar score and neonatal intensive care unit admission. Conclusion: Despite our promising results for older fathers, as paternal age was not associated with clinically relevant obstetrical and perinatal outcomes, future well-designed studies are necessary as it has been associated with other important disorders.<\/jats:p>","DOI":"10.3390\/biology12091256","type":"journal-article","created":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T23:11:52Z","timestamp":1695165112000},"page":"1256","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Obstetrical and Perinatal Outcomes Are Not Associated with Advanced Paternal Age in IVF or ICSI Pregnancies with Autologous Oocytes"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1687-1279","authenticated-orcid":false,"given":"Ana","family":"Navarro-Gomezlechon","sequence":"first","affiliation":[{"name":"IVIRMA Global Research Alliance, IVI Foundation, Instituto de Investigaci\u00f3n Sanitaria La Fe (IIS La Fe), Av. Fernando Abril Martorell, 106, Torre A, 46026 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6863-8981","authenticated-orcid":false,"given":"Mar\u00eda","family":"Gil Juli\u00e1","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, IVI Foundation, Instituto de Investigaci\u00f3n Sanitaria La Fe (IIS La Fe), Av. Fernando Abril Martorell, 106, Torre A, 46026 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa Mar\u00eda","family":"Pacheco-Rend\u00f3n","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, IVI Foundation, Instituto de Investigaci\u00f3n Sanitaria La Fe (IIS La Fe), Av. Fernando Abril Martorell, 106, Torre A, 46026 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Herv\u00e1s","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, IVIRMA Roma, Via Federico Calabresi, 11, 00169 Roma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Mossetti","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, IVIRMA Roma, Via Federico Calabresi, 11, 00169 Roma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roc\u00edo","family":"Rivera-Egea","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, Andrology Laboratory and Sperm Bank, IVIRMA Valencia, Plaza de la Policia Local 3, 46015 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicol\u00e1s","family":"Garrido","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, IVI Foundation, Instituto de Investigaci\u00f3n Sanitaria La Fe (IIS La Fe), Av. Fernando Abril Martorell, 106, Torre A, 46026 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1016\/j.fertnstert.2013.02.006","article-title":"Assessment of Sperm Using MRNA Microarray Technology","volume":"99","author":"Garrido","year":"2013","journal-title":"Fertil. Steril."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1093\/humupd\/dmt031","article-title":"The Presence, Role and Clinical Use of Spermatozoal RNAs","volume":"19","author":"Jodar","year":"2013","journal-title":"Hum. Reprod. Updat."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Cheung, S., Parrella, A., Rosenwaks, Z., and Palermo, G.D. (2019). Genetic and Epigenetic Profiling of the Infertile Male. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0214275"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1093\/humupd\/dmh030","article-title":"Reproductive Functions of the Ageing Male","volume":"10","author":"Nieschlag","year":"2004","journal-title":"Hum. Reprod. Updat."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1136\/jech.2005.045179","article-title":"Advanced Paternal Age: How Old Is Too Old?","volume":"60","author":"Bray","year":"2006","journal-title":"J. Epidemiol. Community Health"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Sharma, R., Agarwal, A., Rohra, V.K., Assidi, M., Abu-Elmagd, M., and Turki, R.F. (2015). Effects of Increased Paternal Age on Sperm Quality, Reproductive Outcome and Associated Epigenetic Risks to Offspring. Reprod. Biol. Endocrinol., 13.","DOI":"10.1186\/s12958-015-0028-x"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1007\/s10815-019-01549-z","article-title":"Advanced Paternal Age Does Not Affect Embryo Aneuploidy Following Blastocyst Biopsy in Egg Donor Cycles","volume":"36","author":"Carrasquillo","year":"2019","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.fertnstert.2020.03.034","article-title":"Is There a Correlation between Paternal Age and Aneuploidy Rate? An Analysis of 3118 Embryos Derived from Young Egg Donors","volume":"114","author":"Dviri","year":"2020","journal-title":"Fertil. Steril."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.fertnstert.2016.12.020","article-title":"Introduction: What to Do with Older Prospective Fathers: The Risks of Advanced Paternal Age","volume":"107","author":"Sigman","year":"2017","journal-title":"Fertil. Steril."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.fertnstert.2016.11.018","article-title":"Management and Counseling of the Male with Advanced Paternal Age","volume":"107","author":"Jennings","year":"2017","journal-title":"Fertil. Steril."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1093\/humrep\/dex267","article-title":"The Age of Fathers in the USA Is Rising: An Analysis of 168 867 480 Births from 1972 to 2015","volume":"32","author":"Khandwala","year":"2017","journal-title":"Hum. Reprod."},{"key":"ref_12","first-page":"1","article-title":"Delayed Childbearing: More Women Are Having Their First Child Later in Life","volume":"21","author":"Matthews","year":"2008","journal-title":"NCHS Data Brief"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ogawa, K., Urayama, K.Y., Tanigaki, S., Sago, H., Sato, S., Saito, S., and Morisaki, N. (2017). Association between Very Advanced Maternal Age and Adverse Pregnancy Outcomes: A Cross Sectional Japanese Study. BMC Pregnancy Childbirth, 17.","DOI":"10.1186\/s12884-017-1540-0"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Londero, A.P., Rossetti, E., Pittini, C., Cagnacci, A., and Driul, L. (2019). Maternal Age and the Risk of Adverse Pregnancy Outcomes: A Retrospective Cohort Study. BMC Pregnancy Childbirth, 19.","DOI":"10.1186\/s12884-019-2400-x"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Li, H., Fan, C., Mubarik, S., Nabi, G., and Ping, Y.X. (2022). The Trend in Delayed Childbearing and Its Potential Consequences on Pregnancy Outcomes: A Single Center 9-Years Retrospective Cohort Study in Hubei, China. BMC Pregnancy Childbirth, 22.","DOI":"10.1186\/s12884-022-04807-8"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1002\/ijgo.14100","article-title":"Maternal and Perinatal According to Maternal Age: A Systematic Review and Meta-Analysis","volume":"159","author":"Saccone","year":"2022","journal-title":"Int. J. Gynecol. Obstet."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1093\/humupd\/dmy005","article-title":"The Effect of Paternal Factors on Perinatal and Paediatric Outcomes: A Systematic Review and Meta-Analysis","volume":"24","author":"Oldereid","year":"2018","journal-title":"Hum. Reprod. Updat."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1093\/humupd\/dmaa010","article-title":"Advanced Paternal Age Is Associated with an Increased Risk of Spontaneous Miscarriage: A Systematic Review and Meta-Analysis","volume":"26","author":"Lashley","year":"2020","journal-title":"Hum. Reprod. Updat."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.fertnstert.2020.04.033","article-title":"Should We Be Worried about Advanced Paternal Age?","volume":"114","author":"Barsky","year":"2020","journal-title":"Fertil. Steril."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1097\/GIM.0b013e318176fabb","article-title":"Statement on Guidance for Genetic Counseling in Advanced Paternal Age","volume":"10","author":"Toriello","year":"2008","journal-title":"Genet. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1093\/humupd\/dmaa046","article-title":"The Other Face of Advanced Paternal Age: A Scoping Review of Its Terminological, Social, Public Health, Psychological, Ethical and Regulatory Aspects","volume":"27","author":"Couture","year":"2021","journal-title":"Hum. Reprod. Updat."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1080\/1464727012000199251","article-title":"The Effects of Female Age on Fecundity and Pregnancy Outcome","volume":"4","author":"Nugent","year":"2001","journal-title":"Hum. Fertil."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s00404-010-1587-x","article-title":"The Impact of Advanced Maternal Age and Parity on Obstetric and Perinatal Outcomes in Singleton Gestations","volume":"284","author":"Wang","year":"2011","journal-title":"Arch. Gynecol. Obstet."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.maturitas.2022.10.008","article-title":"The Effect of Advanced Paternal Age on the Lifespan of Male Offspring in an Ancient Chinese Genealogical Data Set","volume":"168","author":"Wen","year":"2023","journal-title":"Maturitas"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3519","DOI":"10.1007\/s00431-023-05025-w","article-title":"Paternal Age and Risk of Congenital Anomalies and Birth Outcomes: A Population-Based Cohort Study","volume":"182","author":"Bu","year":"2023","journal-title":"Eur. J. Pediatr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.rbmo.2022.03.031","article-title":"Does Advanced Paternal Age Affect Outcomes Following Assisted Reproductive Technology? A Systematic Review and Meta-Analysis","volume":"45","author":"Murugesu","year":"2022","journal-title":"Reprod. Biomed. Online"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1093\/humupd\/dmac033","article-title":"Age-Associated Epigenetic Changes in Mammalian Sperm: Implications for Offspring Health and Development","volume":"29","author":"Ashapkin","year":"2023","journal-title":"Hum. Reprod. Updat."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1007\/s11934-018-0802-3","article-title":"Infertility in the Aging Male","volume":"19","author":"Mazur","year":"2018","journal-title":"Curr. Urol. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.fertnstert.2016.12.021","article-title":"Age-Related Alterations in the Genetics and Genomics of the Male Germ Line","volume":"107","author":"Herati","year":"2017","journal-title":"Fertil. Steril."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"k4372","DOI":"10.1136\/bmj.k4372","article-title":"Association of Paternal Age with Perinatal Outcomes between 2007 and 2016 in the United States: Population Based Cohort Study","volume":"363","author":"Khandwala","year":"2018","journal-title":"BMJ"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0015-0282(00)01679-4","article-title":"Effects of Male Age on Semen Quality and Fertility: A Review of the Literature","volume":"75","author":"Kidd","year":"2001","journal-title":"Fertil. Steril."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/humrep\/deu189","article-title":"Paternal Age and Assisted Reproductive Outcomes in ICSI Donor Oocytes: Is There an Effect of Older Fathers?","volume":"29","author":"Obradors","year":"2014","journal-title":"Hum. Reprod."},{"key":"ref_33","first-page":"26","article-title":"High Percentages of Embryos with 21, 18 or 13 Trisomy Are Related to Advanced Paternal Age in Donor Egg Cycles","volume":"22","author":"Hilario","year":"2018","journal-title":"JBRA Assist. Reprod."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Wu, Y., Kang, X., Zheng, H., Liu, H., Huang, Q., and Liu, J. (2016). Effect of Paternal Age on Reproductive Outcomes of Intracytoplasmic Sperm Injection. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0149867"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/andr.12908","article-title":"Relationship between Male Age, Semen Parameters and Assisted Reproductive Technology Outcomes","volume":"9","author":"Kasman","year":"2020","journal-title":"Andrology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1136\/jfprhc-2013-100866","article-title":"Delayed Fatherhood","volume":"40","author":"Lawson","year":"2014","journal-title":"J. Fam. Plan. Reprod. Health Care"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1097\/01.ede.0000152526.63279.da","article-title":"Paternal Age and Preterm Birth","volume":"16","author":"Zhu","year":"2005","journal-title":"Epidemiology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1097\/01.ede.0000197053.61176.f4","article-title":"Paternal Age and Preterm Birth in Italy, 1990 to 1998","volume":"17","author":"Astolfi","year":"2006","journal-title":"Epidemiology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1136\/jech-2017-210170","article-title":"Paternal Age and the Risk of Low Birth Weight and Preterm Delivery: A Finnish Register-Based Study","volume":"72","author":"Goisis","year":"2018","journal-title":"J. Epidemiol. Community Health"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"E29846","DOI":"10.1097\/MD.0000000000029846","article-title":"Advanced Parental Age Is an Independent Risk Factor for Term Low Birth Weight and Macrosomia","volume":"101","author":"Chung","year":"2022","journal-title":"Medicine"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1097\/01.ede.0000220690.43455.22","article-title":"Paternal Age and Apgar Scores of Newborn Infants","volume":"17","author":"Sun","year":"2006","journal-title":"Epidemiology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"566.e1","DOI":"10.1016\/j.ajog.2017.07.034","article-title":"Influence of Paternal Age on Perinatal Outcomes","volume":"217","author":"Hurley","year":"2017","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1080\/10641950600912992","article-title":"Maternal Age, Paternal Age and New-Onset Hypertension in Late Pregnancy","volume":"25","author":"Chen","year":"2006","journal-title":"Hypertens. Pregnancy"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1093\/humrep\/dem403","article-title":"Paternal Age and Adverse Birth Outcomes: Teenager or 40+, Who Is at Risk?","volume":"23","author":"Chen","year":"2008","journal-title":"Hum. Reprod."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Navarro-Gomezlechon, A., Gil Juli\u00e1, M., Herv\u00e1s, I., Mossetti, L., Rivera-Egea, R., and Garrido, N. (2023). Advanced Paternal Age Does Not Affect Medically-Relevant Obstetrical and Perinatal Outcomes Following IVF or ICSI in Humans with Donated Oocytes. J. Clin. Med., 12.","DOI":"10.3390\/jcm12031014"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1001\/archpsyc.63.9.1026","article-title":"Advancing Paternal Age and Autism","volume":"63","author":"Reichenberg","year":"2006","journal-title":"Arch. Gen. Psychiatry"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.schres.2021.06.016","article-title":"Advanced Paternal Age and Risk of Schizophrenia in Offspring\u2014Review of Epidemiological Findings and Potential Mechanisms","volume":"233","author":"Khachadourian","year":"2021","journal-title":"Schizophr. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1017\/S0033291719000242","article-title":"Understanding the Association between Advanced Paternal Age and Schizophrenia and Bipolar Disorder","volume":"50","author":"Weiser","year":"2020","journal-title":"Psychol. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1158\/1055-9965.EPI-12-0178","article-title":"Parental Age, Family Size, and Offspring\u2019s Risk of Childhood and Adult Acute Leukemia","volume":"21","author":"Larfors","year":"2012","journal-title":"Cancer Epidemiol. Biomark. Prev."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"pkac040","DOI":"10.1093\/jncics\/pkac040","article-title":"Parental Age and Childhood Lymphoma and Solid Tumor Risk: A Literature Review and Meta-Analysis","volume":"6","author":"Domingues","year":"2022","journal-title":"JNCI Cancer Spectr."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/j.1365-3016.2008.00979.x","article-title":"Parental Age as a Risk Factor for Isolated Congenital Malformations in a Polish Population","volume":"23","author":"Mejnartowicz","year":"2009","journal-title":"Paediatr. P\u00e9rinat. Epidemiol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Yoon, S., Qin, J., Glaser, R.L., Jabs, E.W., Wexler, N.S., Sokol, R., Arnheim, N., and Calabrese, P. (2009). The Ups and Downs of Mutation Frequencies during Aging Can Account for the Apert Syndrome Paternal Age Effect. PLoS Genet., 5.","DOI":"10.1371\/journal.pgen.1000558"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1111\/andr.13385","article-title":"Impact of Paternal Age on Assisted Reproductive Technology Outcomes and Offspring Health: A Systematic Review","volume":"11","author":"Gourinat","year":"2023","journal-title":"Andrology"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1002\/tera.1420500111","article-title":"Paternal Age and the Risk of Congenital Heart Defects","volume":"50","author":"Olshan","year":"1994","journal-title":"Teratology"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.1093\/humrep\/deaa105","article-title":"Association between Advanced Paternal Age and Congenital Heart Defects: A Systematic Review and Meta-Analysis","volume":"35","author":"Bertille","year":"2020","journal-title":"Hum. Reprod."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"25373","DOI":"10.18632\/aging.104141","article-title":"Effect of Paternal Age on Offspring Birth Defects: A Systematic Review and Meta-Analysis","volume":"12","author":"Fang","year":"2020","journal-title":"Aging"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1111\/andr.12551","article-title":"High Sperm DNA Fragmentation Delays Human Embryo Kinetics When Oocytes from Young and Healthy Donors Are Microinjected","volume":"6","author":"Esbert","year":"2018","journal-title":"Andrology"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1016\/j.fertnstert.2014.09.001","article-title":"Removal of Annexin V-Positive Sperm Cells for Intracytoplasmic Sperm Injection in Ovum Donation Cycles Does Not Improve Reproductive Outcome: A Controlled and Randomized Trial in Unselected Males","volume":"102","author":"Romany","year":"2014","journal-title":"Fertil. Steril."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.fertnstert.2006.11.162","article-title":"Obesity and Poor Reproductive Outcome: The Potential Role of the Endometrium","volume":"88","author":"Bellver","year":"2007","journal-title":"Fertil. Steril."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.1093\/humrep\/deq146","article-title":"Use of Cryo-Banked Oocytes in an Ovum Donation Programme: A Prospective, Randomized, Controlled, Clinical Trial","volume":"25","author":"Cobo","year":"2010","journal-title":"Hum. Reprod."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1016\/j.fertnstert.2015.08.020","article-title":"Six Years\u2019 Experience in Ovum Donation Using Vitrified Oocytes: Report of Cumulative Outcomes, Impact of Storage Time, and Development of a Predictive Model for Oocyte Survival Rate","volume":"104","author":"Cobo","year":"2015","journal-title":"Fertil. Steril."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.fertnstert.2017.09.022","article-title":"Variables Associated with Mitochondrial Copy Number in Human Blastocysts: What Can We Learn from Trophectoderm Biopsies?","volume":"109","author":"Mifsud","year":"2018","journal-title":"Fertil. Steril."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"89710","DOI":"10.3389\/fendo.2022.897101","article-title":"Advanced Paternal Age and Future Generations","volume":"13","author":"Chan","year":"2022","journal-title":"Front. Endocrinol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Kaltsas, A., Moustakli, E., Zikopoulos, A., Georgiou, I., Dimitriadis, F., Symeonidis, E.N., Markou, E., Michaelidis, T.M., Tien, D.M.B., and Giannakis, I. (2023). Impact of Advanced Paternal Age on Fertility and Risks of Genetic Disorders in Offspring. Genes, 14.","DOI":"10.3390\/genes14020486"}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/12\/9\/1256\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:54:06Z","timestamp":1760129646000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/12\/9\/1256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,20]]},"references-count":64,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["biology12091256"],"URL":"https:\/\/doi.org\/10.3390\/biology12091256","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,20]]}}}