{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:41:50Z","timestamp":1760150510903,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:00:00Z","timestamp":1700006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CIIMAR","award":["UIDB\/04423\/2020","UIDP\/04423\/2020","UID\/MAR\/04292\/2020","LA\/P\/0069\/2020","2021.02559.CEECIND"],"award-info":[{"award-number":["UIDB\/04423\/2020","UIDP\/04423\/2020","UID\/MAR\/04292\/2020","LA\/P\/0069\/2020","2021.02559.CEECIND"]}]},{"name":"MARE","award":["UIDB\/04423\/2020","UIDP\/04423\/2020","UID\/MAR\/04292\/2020","LA\/P\/0069\/2020","2021.02559.CEECIND"],"award-info":[{"award-number":["UIDB\/04423\/2020","UIDP\/04423\/2020","UID\/MAR\/04292\/2020","LA\/P\/0069\/2020","2021.02559.CEECIND"]}]},{"name":"Associate Laboratory ARNET","award":["UIDB\/04423\/2020","UIDP\/04423\/2020","UID\/MAR\/04292\/2020","LA\/P\/0069\/2020","2021.02559.CEECIND"],"award-info":[{"award-number":["UIDB\/04423\/2020","UIDP\/04423\/2020","UID\/MAR\/04292\/2020","LA\/P\/0069\/2020","2021.02559.CEECIND"]}]},{"DOI":"10.13039\/501100001871","name":"FCT researcher contract","doi-asserted-by":"publisher","award":["UIDB\/04423\/2020","UIDP\/04423\/2020","UID\/MAR\/04292\/2020","LA\/P\/0069\/2020","2021.02559.CEECIND"],"award-info":[{"award-number":["UIDB\/04423\/2020","UIDP\/04423\/2020","UID\/MAR\/04292\/2020","LA\/P\/0069\/2020","2021.02559.CEECIND"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Marine Sciences\u2014Marine Resources of the ICBAS\u2014U. Porto","award":["UIDB\/04423\/2020","UIDP\/04423\/2020","UID\/MAR\/04292\/2020","LA\/P\/0069\/2020","2021.02559.CEECIND"],"award-info":[{"award-number":["UIDB\/04423\/2020","UIDP\/04423\/2020","UID\/MAR\/04292\/2020","LA\/P\/0069\/2020","2021.02559.CEECIND"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Fatty acids are energy sources, and their profiles are used as biomarkers of metabolic status and physiological changes in fish. Within this context, the main aim of this study was to identify the fatty acids that best discriminate the reproductive status of male and female farmed brown trout. The fatty acid composition in liver and plasma samples from the adults of both sexes was monitored along four distinct reproductive stages, namely the spawning capable (December), regressing (March), regenerating (July), and developing (November) stages. Irrespective of the sex and stage, the most representative fatty acids were palmitic acid (16:0), stearic acid (18:0), oleic acid (18:1 n-9), arachidonic acid (20:4 n-6), eicosapentaenoic acid (EPA, 20:5 n-3), and docosahexaenoic acid (DHA, 22:6 n-3). There were no significant sex differences in fatty acid classes in the liver and plasma. Despite this, there were several changes in individual fatty acid levels between the sexes. In the liver, both males and females showed high monounsaturated fatty acid and low polyunsaturated fatty acid (PUFA) levels during the regressing and regenerating stages. At spawning capable and developing stages, a reverse profile was noted. The plasma profiles were mainly influenced by changes in saturated fatty acids and PUFAs in males and by PUFA in females. Based on the most representative fatty acids, four patterns were established for female plasma samples, one for each reproductive stage. This scenario suggests that female plasma samples are promising for the discrimination of gonadal reproductive status, and this potential can be further explored in aquaculture and environmental monitoring studies.<\/jats:p>","DOI":"10.3390\/biology12111434","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T01:56:29Z","timestamp":1700099789000},"page":"1434","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Liver and Plasma Fatty Acid Characterization in Cultured Brown Trout at Distinct Reproductive Stages"],"prefix":"10.3390","volume":"12","author":[{"given":"T\u00e2nia Vieira","family":"Madureira","sequence":"first","affiliation":[{"name":"Team of Animal Morphology and Toxicology, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto (U. Porto), Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Laboratory of Histology and Embryology, Department of Microscopy, ICBAS\u2014School of Medicine and Biomedical Sciences, University of Porto (U. Porto), Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0912-3379","authenticated-orcid":false,"given":"Diana","family":"Santos","sequence":"additional","affiliation":[{"name":"Team of Animal Morphology and Toxicology, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto (U. Porto), Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Laboratory of Histology and Embryology, Department of Microscopy, ICBAS\u2014School of Medicine and Biomedical Sciences, University of Porto (U. Porto), Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6107-9790","authenticated-orcid":false,"given":"Tiago","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre & ARNET\u2014Aquatic Research Network Associated Laboratory, ESTM, Polytechnic of Leiria, 2520-641 Peniche, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9887-1864","authenticated-orcid":false,"given":"Marco F. L.","family":"Lemos","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre & ARNET\u2014Aquatic Research Network Associated Laboratory, ESTM, Polytechnic of Leiria, 2520-641 Peniche, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9813-4389","authenticated-orcid":false,"given":"Eduardo","family":"Rocha","sequence":"additional","affiliation":[{"name":"Team of Animal Morphology and Toxicology, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto (U. Porto), Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Laboratory of Histology and Embryology, Department of Microscopy, ICBAS\u2014School of Medicine and Biomedical Sciences, University of Porto (U. Porto), Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"107493","DOI":"10.1016\/j.foodcont.2020.107493","article-title":"Assessment of phospholipid fatty acid profiles for discrimination of salmonids cultured in freshwater and seawater","volume":"120","author":"Han","year":"2021","journal-title":"Food Control"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1080\/09637480701777886","article-title":"Seasonal comparison of wild and farmed brown trout (Salmo trutta forma fario L., 1758): Crude lipid, gonadosomatic index and fatty acids","volume":"60","author":"Kaya","year":"2009","journal-title":"Int. J. Food Sci. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.foodchem.2004.08.026","article-title":"A comparison of the proximate compositions and fatty acid profiles of zander (Sander lucioperca) from two different regions and climatic conditions","volume":"92","author":"Diler","year":"2005","journal-title":"Food Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.foodchem.2006.08.009","article-title":"Fatty acid profiles and fat contents of commercially important seawater and freshwater fish species of Turkey: A comparative study","volume":"103","author":"Alagoz","year":"2007","journal-title":"Food Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1021\/jf104154q","article-title":"Comparison of lipid content and fatty acid composition in the edible meat of wild and cultured freshwater and marine fish and shrimps from China","volume":"59","author":"Li","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/0308-8146(94)90030-2","article-title":"Effects of season and location of catch on the fatty acid compositions of some Australian fish species","volume":"51","author":"Armstrong","year":"1994","journal-title":"Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.aquaculture.2012.08.012","article-title":"Effect of sexual maturation and triploidy on chemical composition and fatty acid content of energy stores in female rainbow trout, Oncorhynchus mykiss","volume":"364\u2013365","author":"Manor","year":"2012","journal-title":"Aquaculture"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s40071-014-0065-7","article-title":"Effects of polyunsaturated fatty acids (PUFAs) on gonadal maturation and spawning of striped gourami, Colisa fasciatus","volume":"6","author":"Hossen","year":"2014","journal-title":"Int. Aquat. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1111\/j.1365-2109.2008.02150.x","article-title":"Fatty acid requirements in ontogeny of marine and freshwater fish","volume":"41","author":"Tocher","year":"2010","journal-title":"Aquac. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.foodchem.2008.05.025","article-title":"A comparative analysis of the fatty acid profiles in the liver and muscles of male and female Salmo trutta macrostigma","volume":"112","author":"Akpinar","year":"2009","journal-title":"Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.plefa.2015.12.003","article-title":"The stability of blood fatty acids during storage and potential mechanisms of degradation: A review","volume":"104","author":"Metherel","year":"2016","journal-title":"Prostaglandins Leukot. Essent. Fatty Acids"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1007\/s10499-011-9468-2","article-title":"The effect of ploidy on the fatty acid profile during the reproductive cycle of female rainbow trout (Oncorhynchus mykiss)","volume":"20","author":"Ribeiro","year":"2012","journal-title":"Aquac. Int."},{"key":"ref_13","first-page":"27","article-title":"Seasonal variation of total lipid and total fatty acid in muscle and liver of rainbow trout (Oncorhynchus mykiss W., 1792) reared in Derbent Dam Lake","volume":"7","author":"Kandemir","year":"2007","journal-title":"Turk. J. Fish. Aquat. Sci."},{"key":"ref_14","first-page":"1025","article-title":"Seasonal variations in the fatty acid profiles of the liver and muscle of Squalius cephalus (Teleostei: Cyprinidae) living in T\u00f6d\u00fcrge Lake (Sivas, Turkiye)","volume":"18","year":"2019","journal-title":"Iran J. Fish Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1111\/j.1600-0633.2006.00174.x","article-title":"Variations in fatty acids composition of pikeperch (Sander lucioperca) liver with respect to gonad maturation","volume":"15","author":"Uysal","year":"2006","journal-title":"Ecol. Freshw. Fish"},{"key":"ref_16","first-page":"166","article-title":"Comparative study of lipid and fatty acid profile in liver tissues of male and female Silurus triostegus during the catching seasons","volume":"36","year":"2021","journal-title":"Aquat. Sci. Eng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.aquaculture.2008.10.043","article-title":"Effects of fatty acid composition and spawning season patterns on egg quality and larval survival in common snook (Centropomus undecimalis)","volume":"287","author":"Rhody","year":"2009","journal-title":"Aquaculture"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Santos, D., Rocha, E., Malh\u00e3o, F., Lopes, C., Gon\u00e7alves, J.F., and Madureira, T.V. (2021). Multi-parametric portfolio to assess the fitness and gonadal maturation in four key reproductive phases of brown trout. Animals, 11.","DOI":"10.3390\/ani11051290"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1080\/19425120.2011.555724","article-title":"A standardized terminology for describing reproductive development in fishes","volume":"3","author":"Wyanski","year":"2011","journal-title":"Mar. Coast. Fish."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cbpc.2018.06.001","article-title":"Sex-steroids and hypolipidemic chemicals impacts on brown trout lipid and peroxisome signaling\u2014Molecular, biochemical and morphological insights","volume":"212","author":"Madureira","year":"2018","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.scitotenv.2017.02.015","article-title":"Fatty acid profile of the sea snail Gibbula umbilicalis as a biomarker for coastal metal pollution","volume":"586","author":"Silva","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Silva, C.O., Sim\u00f5es, T., F\u00e9lix, R., Soares, A.M.V.M., Barata, C., Novais, S.C., and Lemos, M.F.L. (2021). Asparagopsis armata exudate cocktail: The quest for the mechanisms of toxic action of an invasive seaweed on marine invertebrates. Biology, 10.","DOI":"10.3390\/biology10030223"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Guo, X. (2014). Advances in Gas Chromatography, IntechOpen. Chapter 2.","DOI":"10.5772\/57016"},{"key":"ref_24","first-page":"1","article-title":"PAST: Paleontological statistics software package for education and data analysis","volume":"4","author":"Hammer","year":"2001","journal-title":"Palaeontol. Electron."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"117","DOI":"10.4194\/2618-6381-v21_3_04","article-title":"Comparative analysis of egg biochemical composition and egg productivity rainbow trout (Oncorhynchus mykiss Walbaum, 1792) in different stations in Turkey","volume":"21","author":"Baki","year":"2021","journal-title":"Aquac. Stud."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1051\/rnd:19850409","article-title":"Incidence of (n-3) essential fatty acid deficiency on trout reproductive processes","volume":"25","author":"Leray","year":"1985","journal-title":"Reprod. Nutr. Dev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.ygcen.2008.07.017","article-title":"An investigation into the role of prostaglandins in zebrafish oocyte maturation and ovulation","volume":"159","author":"Lister","year":"2008","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1095\/biolreprod64.1.382","article-title":"Effects of polyunsaturated fatty acids and prostaglandins on oocyte maturation in a marine teleost, the European Sea Bass (Dicentrarchus labrax)","volume":"64","author":"Carrillo","year":"2001","journal-title":"Biol. Reprod."},{"key":"ref_29","first-page":"311","article-title":"Comparison of fatty acid profiles of different tissues of mature trout (Salmo trutta labrax, Pallas, 1811) caught from Kazandere Creek in the \u00c7oruh region, Erzurum, Turkey","volume":"27","author":"Aras","year":"2003","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"ref_30","first-page":"613","article-title":"Seasonal variations of proximate and total fatty acid composition of wild brown trout in Munzur river, Tunceli-Turkey","volume":"13","author":"Can","year":"2013","journal-title":"Turk. J. Fish. Aquat. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1515\/tjb-2017-0045","article-title":"Variations in the fatty acid compositions of the liver and gonad tissue of spiny eel (Mastacembelus mastacembelus) from Atat\u00fcrk Dam Lake","volume":"42","year":"2017","journal-title":"Turk. J. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.cbpb.2006.11.023","article-title":"Comparison of fatty acid profiles of spawning and non-spawning Pacific herring, Clupea harengus pallasi","volume":"146","author":"Huynh","year":"2007","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.jfca.2016.11.005","article-title":"A comparative analysis of lipid content and fatty acid composition in muscle, liver and gonads of Seriola fasciata Bloch 1793 based on gender and maturation stage","volume":"59","author":"Nogueira","year":"2017","journal-title":"J. Food Compost. Anal."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1016\/j.foodchem.2009.07.064","article-title":"Fatty acids of neutral and phospholipids of three endangered trout: Salmo trutta caspius Kessler, Salmo trutta labrax Pallas and Salmo trutta macrostigma Dumeril","volume":"119","author":"Aksakal","year":"2010","journal-title":"Food Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1007\/s10695-008-9220-7","article-title":"Changes in some components of the muscle lipids of three freshwater fish species under natural extreme cold and temperate conditions","volume":"34","author":"Uysal","year":"2008","journal-title":"Fish Physiol. Biochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1007\/s11802-019-3775-z","article-title":"Temperature-dependent fatty acid composition change of phospholipid in steelhead trout (Oncorhynchus mykiss) tissues","volume":"18","author":"Liu","year":"2019","journal-title":"J. Ocean Univ. China"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"R91","DOI":"10.1152\/ajpregu.1979.236.1.R91","article-title":"Influence of thermal acclimation on membrane lipid composition of rainbow trout liver","volume":"236","author":"Hazel","year":"1979","journal-title":"Am. J. Physiol.\u2014Regul. Integr. Comp. Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1111\/j.1439-0426.2007.00874.x","article-title":"Reproductive performance of carp, Catla catla (Ham.), reared on a formulated diet with PUFA supplementation","volume":"23","author":"Nandi","year":"2007","journal-title":"J. Appl. Ichthyol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1046\/j.1444-2906.2001.00328.x","article-title":"Quality of eggs and spermatozoa of rainbow trout fed an n-3 essential fatty acid-deficient diet and its effects on the lipid and fatty acid components of eggs, semen and livers","volume":"67","author":"Watanabe","year":"2001","journal-title":"Fish. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.foodchem.2004.07.001","article-title":"Influence of season on the lipid content and fatty acid profiles of three tilapia species (Oreochromis niloticus, O. macrochir and Tilapia rendalli) from Madagascar","volume":"91","author":"Rasoarahona","year":"2005","journal-title":"Food Chem."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/12\/11\/1434\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:23:44Z","timestamp":1760131424000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/12\/11\/1434"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,15]]},"references-count":40,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["biology12111434"],"URL":"https:\/\/doi.org\/10.3390\/biology12111434","relation":{},"ISSN":["2079-7737"],"issn-type":[{"type":"electronic","value":"2079-7737"}],"subject":[],"published":{"date-parts":[[2023,11,15]]}}}