{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T19:34:20Z","timestamp":1791833660052,"version":"4.3.4"},"reference-count":47,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,1,6]],"date-time":"2024-01-06T00:00:00Z","timestamp":1704499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Research and Development Program of Hubei Province","award":["2021BBA230"],"award-info":[{"award-number":["2021BBA230"]}]},{"name":"Key Research and Development Program of Hubei Province","award":["2023YFD2401802"],"award-info":[{"award-number":["2023YFD2401802"]}]},{"name":"Key Research and Development Program of Hubei Province","award":["2019331"],"award-info":[{"award-number":["2019331"]}]},{"name":"Key Research and Development Program of Hubei Province","award":["2018ABA102"],"award-info":[{"award-number":["2018ABA102"]}]},{"name":"Key Research and Development Program of Hubei Province","award":["jxsq2020102109"],"award-info":[{"award-number":["jxsq2020102109"]}]},{"name":"National Key Research and Development Program of China","award":["2021BBA230"],"award-info":[{"award-number":["2021BBA230"]}]},{"name":"National Key Research and Development Program of China","award":["2023YFD2401802"],"award-info":[{"award-number":["2023YFD2401802"]}]},{"name":"National Key Research and Development Program of China","award":["2019331"],"award-info":[{"award-number":["2019331"]}]},{"name":"National Key Research and Development Program of China","award":["2018ABA102"],"award-info":[{"award-number":["2018ABA102"]}]},{"name":"National Key Research and Development Program of China","award":["jxsq2020102109"],"award-info":[{"award-number":["jxsq2020102109"]}]},{"name":"Youth Innovation Promotion Association CAS","award":["2021BBA230"],"award-info":[{"award-number":["2021BBA230"]}]},{"name":"Youth Innovation Promotion Association CAS","award":["2023YFD2401802"],"award-info":[{"award-number":["2023YFD2401802"]}]},{"name":"Youth Innovation Promotion Association CAS","award":["2019331"],"award-info":[{"award-number":["2019331"]}]},{"name":"Youth Innovation Promotion Association CAS","award":["2018ABA102"],"award-info":[{"award-number":["2018ABA102"]}]},{"name":"Youth Innovation Promotion Association CAS","award":["jxsq2020102109"],"award-info":[{"award-number":["jxsq2020102109"]}]},{"name":"Technical Innovation Project of the Science and Technology Department of Hubei Province","award":["2021BBA230"],"award-info":[{"award-number":["2021BBA230"]}]},{"name":"Technical Innovation Project of the Science and Technology Department of Hubei Province","award":["2023YFD2401802"],"award-info":[{"award-number":["2023YFD2401802"]}]},{"name":"Technical Innovation Project of the Science and Technology Department of Hubei Province","award":["2019331"],"award-info":[{"award-number":["2019331"]}]},{"name":"Technical Innovation Project of the Science and Technology Department of Hubei Province","award":["2018ABA102"],"award-info":[{"award-number":["2018ABA102"]}]},{"name":"Technical Innovation Project of the Science and Technology Department of Hubei Province","award":["jxsq2020102109"],"award-info":[{"award-number":["jxsq2020102109"]}]},{"name":"Innovative and Entrepreneurial Talents Project of Jiangxi Province","award":["2021BBA230"],"award-info":[{"award-number":["2021BBA230"]}]},{"name":"Innovative and Entrepreneurial Talents Project of Jiangxi Province","award":["2023YFD2401802"],"award-info":[{"award-number":["2023YFD2401802"]}]},{"name":"Innovative and Entrepreneurial Talents Project of Jiangxi Province","award":["2019331"],"award-info":[{"award-number":["2019331"]}]},{"name":"Innovative and Entrepreneurial Talents Project of Jiangxi Province","award":["2018ABA102"],"award-info":[{"award-number":["2018ABA102"]}]},{"name":"Innovative and Entrepreneurial Talents Project of Jiangxi Province","award":["jxsq2020102109"],"award-info":[{"award-number":["jxsq2020102109"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>The red swamp crayfish Procambarus clarkii is the most reared shrimp in China, but it is often affected by hypoxia stress in the process of seedling culture and adult crayfish culture. The oxygen consumption rate and asphyxiation point of juvenile crayfish (1.17 \u00b1 0.03 g) and subadult crayfish (11.68 \u00b1 0.11 g) at different temperatures (20, 22, 24, 26, and 28 \u00b0C) were studied. The survival, glycolysis, and expression of antioxidant genes were compared under 24 h acute hypoxia stress (1, 2, and 3 mg\/L) and normal dissolved oxygen (7.5 mg\/L). The results showed that the oxygen consumption rate and asphyxiation point of juvenile and subadult crayfish increased with increasing temperatures (20\u201328 \u00b0C). At the same temperature, the oxygen consumption rate and asphyxiation point of juvenile crayfish were significantly higher than those of subadult crayfish (p &lt; 0.05). Within 24 h, the three hypoxia stress environments did not lead to the death of crayfish, indicating that P. clarkii has a strong ability to adapt to hypoxia. Hypoxia stress significantly affected the activities of antioxidant and anaerobic metabolic enzymes and gene expression in juvenile and subadult crayfish. The activities of the superoxide dismutase (SOD), catalase (CAT), and lactate dehydrogenase (LDH) and the content of lactic acid (LD) in the hepatopancreas of juvenile and subadult crayfish in the hypoxia stress groups increased significantly. The expression levels of SOD mRNA, CAT mRNA, Hsp70 mRNA, and crustin 4 mRNA in the hepatopancreas of juvenile and subadult crayfish in the hypoxia stress groups were significantly higher than those in the control group (p &lt; 0.05), and the higher the degree of hypoxia stress, the higher the expression of each gene. The results showed that the antioxidant system of juvenile crayfish was more sensitive to hypoxia environments, and hypoxia stress resulted in increased stress levels in juvenile crayfish and subadult crayfish.<\/jats:p>","DOI":"10.3390\/biology13010033","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T05:21:38Z","timestamp":1704691298000},"page":"33","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Effects of Hypoxia Stress on Survival, Antioxidant and Anaerobic Metabolic Enzymes, and Related Gene Expression of Red Swamp Crayfish Procambarus clarkii"],"prefix":"10.3390","volume":"13","author":[{"given":"Qinghui","family":"Zeng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingzhong","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangtze University, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lirong","family":"Qin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiashou","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanglin","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangpeng","family":"Feng","sequence":"additional","affiliation":[{"name":"Jiangxi Institute for Fisheries Sciences, Poyang Lake Fisheries Research Centre of Jiangxi Province, Nanchang 330039, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1258-2410","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,6]]},"reference":[{"key":"ref_1","first-page":"22","article-title":"Problems and industrial development of Procambarus clarkii","volume":"08","author":"Shu","year":"2010","journal-title":"China Fish."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"161520","DOI":"10.1016\/j.scitotenv.2023.161520","article-title":"Integrated rice-crayfish farming system does not mitigate the global warming potential during rice season","volume":"867","author":"Guo","year":"2023","journal-title":"Sci. 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