{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T03:42:40Z","timestamp":1789098160707,"version":"build-2803163510"},"reference-count":86,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T00:00:00Z","timestamp":1705363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Charles Sturt University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>As research on parasitic helminths has entered the post-genomic era, research efforts have turned to deciphering the function of genes in the public databases of genome sequences. It is hoped that, by understanding the role of parasite genes in maintaining their parasitic lifestyle, critical insights can be gained to develop new intervention and control strategies. Methods to manipulate and transform parasitic worms are now developed to a point where it has become possible to gain a comprehensive understanding of the molecular mechanisms underlying host\u2013parasite interplay, and here, we summarise and discuss the advances that have been made in schistosome transgenesis over the past 25 years. The ability to genetically manipulate schistosomes holds promise in finding new ways to control schistosomiasis, which ultimately may lead to the eradication of this debilitating disease.<\/jats:p>","DOI":"10.3390\/biology13010048","type":"journal-article","created":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T08:12:37Z","timestamp":1705392757000},"page":"48","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Schistosome Transgenesis: The Long Road to Success"],"prefix":"10.3390","volume":"13","author":[{"given":"Bernd H.","family":"Kalinna","sequence":"first","affiliation":[{"name":"Rural Health Research Institute, Charles Sturt University, Orange, NSW 2800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allen G.","family":"Ross","sequence":"additional","affiliation":[{"name":"Rural Health Research Institute, Charles Sturt University, Orange, NSW 2800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9624-4370","authenticated-orcid":false,"given":"Anna K.","family":"Walduck","sequence":"additional","affiliation":[{"name":"Rural Health Research Institute, Charles Sturt University, Orange, NSW 2800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,16]]},"reference":[{"key":"ref_1","first-page":"153","article-title":"Schistosomiasis: Still a Cause of Significant Morbidity and Mortality","volume":"10","author":"Verjee","year":"2019","journal-title":"Res. Rep. Trop. Med."},{"key":"ref_2","unstructured":"WHO (2020). Ending the Neglect to Attain the Sustainable Development Goals: A Road Map for Neglected Tropical Diseases 2021\u20132030, World Health Organization."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2492","DOI":"10.3389\/fimmu.2018.02492","article-title":"Schistosoma \u201cEggs-Iting\u201d the Host: Granuloma Formation and Egg Excretion","volume":"9","author":"Schwartz","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Masamba, P., and Kappo, A.P. (2021). Immunological and Biochemical Interplay between Cytokines, Oxidative Stress and Schistosomiasis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22137216"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e12702","DOI":"10.1111\/pim.12702","article-title":"Schistosoma haematobium Infection Modulates Plasmodium falciparum Parasite Density and Antimalarial Antibody Responses","volume":"42","author":"Tokplonou","year":"2020","journal-title":"Parasite Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1007\/s11686-020-00330-y","article-title":"Impact of Coinfection with Schistosoma mansoni on the Antibody Response to Helicobacter pylori","volume":"66","author":"Ahmed","year":"2021","journal-title":"Acta Parasitol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Muir, R., Metcalf, T., Fourati, S., Bartsch, Y., Kyosiimire-Lugemwa, J., Canderan, G., Alter, G., Muyanja, E., Okech, B., and Namatovu, T. (2023). Schistosoma mansoni Infection Alters the Host Pre-Vaccination Environment Resulting in Blunted Hepatitis B Vaccination Immune Responses. PLoS Negl. Trop. Dis., 17.","DOI":"10.1371\/journal.pntd.0011089"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e00275-19","DOI":"10.1128\/IAI.00275-19","article-title":"Schistosoma mansoni Worm Infection Regulates the Intestinal Microbiota and Susceptibility to Colitis","volume":"87","author":"Floudas","year":"2019","journal-title":"Infect. Immun."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Li, Z., Wang, X., Zhang, W., Yang, W., Xu, B., and Hu, W. (2023). Excretory\/Secretory Products from Schistosoma japonicum Eggs Alleviate Ovalbumin-Induced Allergic Airway Inflammation. PLoS Negl. Trop. Dis., 17.","DOI":"10.1371\/journal.pntd.0011625"},{"key":"ref_10","unstructured":"CDC (2023, July 11). Schistosomiasis, Available online: https:\/\/www.cdc.gov\/dpdx\/schistosomiasis\/index.html."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.ijpara.2014.05.005","article-title":"A Multi-Component Integrated Approach for the Elimination of Schistosomiasis in the People\u2019s Republic of China: Design and Baseline Results of a 4-Year Cluster-Randomised Intervention Trial","volume":"44","author":"Gray","year":"2014","journal-title":"Int. J. Parasitol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2220","DOI":"10.1016\/S0140-6736(14)61417-3","article-title":"An Audacious Goal: The Elimination of Schistosomiasis in Our Lifetime through Mass Drug Administration","volume":"385","author":"Ross","year":"2015","journal-title":"Lancet"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1093\/infdis\/jiu416","article-title":"Can Mass Drug Administration Lead to the Sustainable Control of Schistosomiasis?","volume":"211","author":"Ross","year":"2015","journal-title":"J. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Clements, A.C.A., Bosqu\u00e9-Oliva, E., Sacko, M., Landour\u00e9, A., Demb\u00e9l\u00e9, R., Traor\u00e9, M., Coulibaly, G., Gabrielli, A.F., Fenwick, A., and Brooker, S. (2009). A Comparative Study of the Spatial Distribution of Schistosomiasis in Mali in 1984\u20131989 and 2004\u20132006. PLoS Negl. Trop. Dis., 3.","DOI":"10.1371\/journal.pntd.0000431"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1017\/S0031182009990369","article-title":"Present and Future Schistosomiasis Control Activities with Support from the Schistosomiasis Control Initiative in West Africa","volume":"136","author":"Garba","year":"2009","journal-title":"Parasitology"},{"key":"ref_16","first-page":"119","article-title":"Current Understanding of Immunity Against Schistosomiasis: Impact on Vaccine and Drug Development","volume":"11","author":"Molehin","year":"2020","journal-title":"Res. Rep. Trop. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.pt.2009.05.011","article-title":"Immune Effector Mechanisms against Schistosomiasis: Looking for a Chink in the Parasite\u2019s Armour","volume":"25","author":"Wilson","year":"2009","journal-title":"Trends Parasitol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Osakunor, D.N.M., Woolhouse, M.E.J., and Mutapi, F. (2018). Paediatric Schistosomiasis: What We Know and What We Need to Know. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006144"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4264","DOI":"10.1128\/IAI.00641-12","article-title":"Progressive Cross-Reactivity in IgE Responses: An Explanation for the Slow Development of Human Immunity to Schistosomiasis?","volume":"80","author":"Fitzsimmons","year":"2012","journal-title":"Infect. Immun."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Van Ooij, C., Tamez, P., Bhattacharjee, S., Hiller, N.L., Harrison, T., Liolios, K., Kooij, T., Ramesar, J., Balu, B., and Adams, J. (2008). The Malaria Secretome: From Algorithms to Essential Function in Blood Stage Infection. PLoS Pathog., 4.","DOI":"10.1371\/annotation\/2b000375-e083-46ed-a44a-ff297e6c37d0"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Homann, O.R., Dea, J., Noble, S.M., and Johnson, A.D. (2009). A Phenotypic Profile of the Candida Albicans Regulatory Network. PLoS Genet., 5.","DOI":"10.1371\/journal.pgen.1000783"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2308","DOI":"10.1101\/gr.097097.109","article-title":"Simultaneous Assay of Every Salmonella Typhi Gene Using One Million Transposon Mutants","volume":"19","author":"Langridge","year":"2009","journal-title":"Genome Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/nature08140","article-title":"The Schistosoma japonicum Genome Reveals Features of Host\u2013Parasite Interplay","volume":"460","author":"Zhou","year":"2009","journal-title":"Nature"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"8687","DOI":"10.1073\/pnas.96.15.8687","article-title":"Transient Expression of DNA and RNA in Parasitic Helminths by Using Particle Bombardment","volume":"96","author":"Davis","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0166-6851(01)00446-7","article-title":"HSP70-Controlled GFP Expression in Transiently Transformed Schistosomes","volume":"120","author":"Wippersteg","year":"2002","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/S0020-7519(02)00092-9","article-title":"Characterisation of the Cysteine Protease ER60 in Transgenic Schistosoma mansoni Larvae","volume":"32","author":"Wippersteg","year":"2002","journal-title":"Int. J. Parasitol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1016\/S0020-7519(03)00168-1","article-title":"The Uptake of Texas Red-BSA in the Excretory System of Schistosomes and Its Colocalisation with ER60 Promoter-Induced GFP in Transiently Transformed Adult Males","volume":"33","author":"Wippersteg","year":"2003","journal-title":"Int. J. Parasitol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.ijpara.2005.02.002","article-title":"Biolistic Transformation of Schistosoma mansoni with 5\u2032 Flanking Regions of Two Peptidase Genes Promotes Tissue-Specific Expression","volume":"35","author":"Wippersteg","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.molbiopara.2009.11.001","article-title":"Biolistic Transformation of Schistosoma mansoni: Studies with Modified Reporter-Gene Constructs Containing Regulatory Regions of Protease Genes","volume":"170","author":"Beckmann","year":"2010","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0166-6851(03)00158-0","article-title":"Cloning of 5\u2032 and 3\u2032 Flanking Regions of the Schistosoma mansoni Calcineurin A Gene and Their Characterization in Transiently Transformed Parasites","volume":"130","author":"Rossi","year":"2003","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.exppara.2003.11.001","article-title":"Schistosoma mansoni Miracidia Transformed by Particle Bombardment Infect Biomphalaria Glabrata Snails and Develop into Transgenic Sporocysts","volume":"105","author":"Heyers","year":"2003","journal-title":"Exp. Parasitol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.exppara.2007.04.007","article-title":"Schistosoma mansoni: Germ-Line Transformation Approaches and Actin-Promoter Analysis","volume":"117","author":"Beckmann","year":"2007","journal-title":"Exp. Parasitol."},{"key":"ref_33","unstructured":"WormBase Parasite (2023, November 07). Schistosoma\u00a0mansoni. Available online: https:\/\/parasite.wormbase.org\/Schistosoma_mansoni_prjea36577\/Info\/Index."},{"key":"ref_34","unstructured":"WormBase Parasite (2023, November 07). Schistosoma\u00a0japonicum. Available online: https:\/\/parasite.wormbase.org\/Schistosoma_japonicum_prjna520774\/Info\/Index."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.pt.2006.10.002","article-title":"Oming in on Schistosomes: Prospects and Limitations for Post-Genomics","volume":"23","author":"Wilson","year":"2007","journal-title":"Trends Parasitol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.exppara.2007.06.005","article-title":"Schistosoma mansoni Genome: Closing in on a Final Gene Set","volume":"117","author":"Haas","year":"2007","journal-title":"Exp. Parasitol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S1471-4922(02)00061-2","article-title":"Mobile Genetic Elements Colonizing the Genomes of Metazoan Parasites","volume":"19","author":"Brindley","year":"2003","journal-title":"Trends Parasitol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1146\/annurev-genet-040620-022145","article-title":"A Field Guide to Eukaryotic Transposable Elements","volume":"54","author":"Wells","year":"2020","journal-title":"Annu. Rev. Genet."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0378-1119(00)00601-6","article-title":"Gulliver, a Long Terminal Repeat Retrotransposon from the Genome of the Oriental Blood Fluke Schistosoma japonicum","volume":"264","author":"Laha","year":"2001","journal-title":"Gene"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4950","DOI":"10.1128\/JVI.78.9.4950.2004","article-title":"Saci-1, -2, and -3 and Perere, Four Novel Retrotransposons with High Transcriptional Activities from the Human Parasite Schistosoma mansoni","volume":"78","author":"DeMarco","year":"2004","journal-title":"J. Virol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1016\/j.ijpara.2004.08.007","article-title":"The Fugitive LTR Retrotransposon from the Genome of the Human Blood Fluke, Schistosoma mansoni","volume":"34","author":"Laha","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1093\/molbev\/msh188","article-title":"Merlin, a New Superfamily of DNA Transposons Identified in Diverse Animal Genomes and Related to Bacterial IS1016 Insertion Sequences","volume":"21","author":"Feschotte","year":"2004","journal-title":"Mol. Biol. Evol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1186\/1471-2148-6-89","article-title":"SmTRC1, a Novel Schistosoma mansoni DNA Transposon, Discloses New Families of Animal and Fungi Transposons Belonging to the CACTA Superfamily","volume":"6","author":"DeMarco","year":"2006","journal-title":"BMC Evol. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"6153","DOI":"10.1128\/JVI.77.11.6153-6166.2003","article-title":"Boudicca, a Retrovirus-like Long Terminal Repeat Retrotransposon from the Genome of the Human Blood Fluke Schistosoma mansoni","volume":"77","author":"Copeland","year":"2003","journal-title":"J. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Llorens, C., Mu\u00f1oz-Pomer, A., Bernad, L., Botella, H., and Moya, A. (2009). Network Dynamics of Eukaryotic LTR Retroelements beyond Phylogenetic Trees. Biol. Direct, 4.","DOI":"10.1186\/1745-6150-4-41"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1093\/oxfordjournals.molbev.a003933","article-title":"CsRn1, a Novel Active Retrotransposon in a Parasitic Trematode, Clonorchis sinensis, Discloses a New Phylogenetic Clade of Ty3\/Gypsy-like LTR Retrotransposons","volume":"18","author":"Bae","year":"2001","journal-title":"Mol. Biol. Evol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1007\/s004380000270","article-title":"Molecular Structure of a Novel Gypsy-Ty3-like Retrotransposon (Kabuki) and Nested Retrotransposable Elements on the W Chromosome of the Silkworm Bombyx Mori","volume":"263","author":"Abe","year":"2000","journal-title":"Mol. Gen. Genet. MGG"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/S0168-9525(99)01777-1","article-title":"Resident Aliens: The Tc1\/Mariner Superfamily of Transposable Elements","volume":"15","author":"Plasterk","year":"1999","journal-title":"Trends Genet."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1017\/S0031182007003824","article-title":"Transgenesis of Schistosomes: Approaches Employing Mobile Genetic Elements","volume":"135","author":"Mann","year":"2008","journal-title":"Parasitology"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/s00438-006-0181-1","article-title":"Both Sense and Antisense Strands of the LTR of the Schistosoma mansoni Pao-like Retrotransposon Sinbad Drive Luciferase Expression","volume":"277","author":"Copeland","year":"2007","journal-title":"Mol. Genet. Genom."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.1096\/fj.07-8726com","article-title":"PiggyBac Transposon Mediated Transgenesis of the Human Blood Fluke, Schistosoma mansoni","volume":"21","author":"Morales","year":"2007","journal-title":"Faseb. J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"7520","DOI":"10.1073\/pnas.95.13.7520","article-title":"The Lepidopteran Transposon Vector, PiggyBac, Mediates Germ-Line Transformation in the Mediterranean Fruit Fly","volume":"95","author":"Handler","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/S0020-7519(03)00030-4","article-title":"Suppression of Cathepsin B Expression in Schistosoma mansoni by RNA Interference","volume":"33","author":"Skelly","year":"2003","journal-title":"Int. J. Parasitol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.molbiopara.2007.03.006","article-title":"Optimizing Gene Suppression in Schistosomes Using RNA Interference","volume":"153","author":"Radwanska","year":"2007","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.molbiopara.2005.06.007","article-title":"Long-Term Suppression of Cathepsin B Levels by RNA Interference Retards Schistosome Growth","volume":"143","author":"Correnti","year":"2005","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3739","DOI":"10.1007\/s00018-010-0345-3","article-title":"Vector-Based RNA Interference of Cathepsin B1 in Schistosoma mansoni","volume":"67","author":"Tchoubrieva","year":"2010","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/978-1-4939-1438-8_8","article-title":"Gene Suppression in Schistosomes Using RNAi","volume":"1201","author":"Skelly","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"247036","DOI":"10.5402\/2013\/247036","article-title":"Applications of RNA Interference in Schistosomiasis: Gene Function Identification and Development of New Therapies","volume":"2013","author":"Pereira","year":"2013","journal-title":"ISRN Parasitol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1016\/j.bbrc.2018.06.109","article-title":"RNA Interference in Vivo in Schistosoma japonicum: Establishing and Optimization of RNAi Mediated Suppression of Gene Expression by Long DsRNA in the Intra-Mammalian Life Stages of Worms","volume":"503","author":"Li","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1186\/s13071-022-05247-1","article-title":"A New Ferritin SjFer0 Affecting the Growth and Development of Schistosoma Japonicum","volume":"15","author":"Zeng","year":"2022","journal-title":"Parasit. Vectors"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Yang, W.-B., Luo, F., Zhang, W., Sun, C.-S., Tan, C., Zhou, A., and Hu, W. (2023). Inhibition of Signal Peptidase Complex Expression Affects the Development and Survival of Schistosoma japonicum. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1136056"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1038\/nature11924","article-title":"Adult Somatic Stem Cells in the Human Parasite Schistosoma mansoni","volume":"494","author":"Collins","year":"2013","journal-title":"Nature"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1017\/S0031182000053920","article-title":"Schistosoma mansoni: Structural Damage and Tegumental Repair after In Vivo Treatment with Praziquantel","volume":"94","author":"Shaw","year":"1987","journal-title":"Parasitology"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Mour\u00e3o, M.M., Dinguirard, N., Franco, G.R., and Yoshino, T.P. (2009). Phenotypic Screen of Early-Developing Larvae of the Blood Fluke, Schistosoma Mansoni, Using RNA Interference. PLoS. Negl. Trop. Dis., 3.","DOI":"10.1371\/annotation\/ffd29b50-4102-46b9-b6a1-cdb4984df278"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Stefani\u0107, S., Dvo\u0159\u00e1k, J., Horn, M., Braschi, S., Sojka, D., Ruelas, D.S., Suzuki, B., Lim, K.-C., Hopkins, S.D., and McKerrow, J.H. (2010). RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening. PLoS. Negl. Trop. Dis., 4.","DOI":"10.1371\/journal.pntd.0000850"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1126\/science.abb7699","article-title":"Large-Scale RNAi Screening Uncovers Therapeutic Targets in the Parasite Schistosoma mansoni","volume":"369","author":"Wang","year":"2020","journal-title":"Science"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"5375","DOI":"10.1038\/ncomms6375","article-title":"Omega-1 Knockdown in Schistosoma mansoni Eggs by Lentivirus Transduction Reduces Granuloma Size In Vivo","volume":"5","author":"Hagen","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"e41337","DOI":"10.7554\/eLife.41337","article-title":"Programmed Genome Editing of the Omega-1 Ribonuclease of the Blood Fluke, Schistosoma mansoni","volume":"8","author":"Ittiprasert","year":"2018","journal-title":"eLife"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"You, H., Liu, C., Du, X., Nawaratna, S., Rivera, V., Harvie, M., Jones, M., and McManus, D.P. (2018). Suppression of Schistosoma japonicum Acetylcholinesterase Affects Parasite Growth and Development. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19082426"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"e21205","DOI":"10.1096\/fj.202001745RR","article-title":"CRISPR\/Cas9-mediated Genome Editing of Schistosoma mansoni Acetylcholinesterase","volume":"35","author":"You","year":"2021","journal-title":"FASEB J."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"3446","DOI":"10.7150\/thno.20359","article-title":"RSj16 Protects against DSS-Induced Colitis by Inhibiting the PPAR-\u03b1 Signaling Pathway","volume":"7","author":"Wang","year":"2017","journal-title":"Theranostics"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"e22615","DOI":"10.1096\/fj.202200600RR","article-title":"CRISPR\/Cas9-mediated Gene Knockout of Sj16 in Schistosoma japonicum Eggs Upregulates the Host-to-egg Immune Response","volume":"36","author":"Zhang","year":"2022","journal-title":"FASEB J."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"83","DOI":"10.4269\/ajtmh.1994.51.83","article-title":"Drug-Resistant Schistosomiasis: Resistance to Praziquantel and Oxamniquine Induced in Schistosoma mansoni in Mice Is Drug Specific","volume":"51","author":"Fallon","year":"1994","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"932","DOI":"10.4269\/ajtmh.1999.60.932","article-title":"Resistance to Praziquantel: Direct Evidence from Schistosoma mansoni Isolated from Egyptian Villagers","volume":"60","author":"Ismail","year":"1999","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1017\/S0031182000007137","article-title":"Praziquantel-Induced Tegumental Damage In Vitro Is Diminished in Schistosomes Derived from Praziquantel-Resistant Infections","volume":"122","author":"Williams","year":"2001","journal-title":"Parasitology"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1097\/00001432-200012000-00014","article-title":"Praziquantel: Is There Real Resistance and Are There Alternatives?","volume":"13","author":"Cioli","year":"2000","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.exppara.2007.06.003","article-title":"Glycomics-Driven Discoveries in Schistosome Research","volume":"117","author":"Hokke","year":"2007","journal-title":"Exp. Parasitol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1074\/mcp.M115.048280","article-title":"Glycomic Analysis of Life Stages of the Human Parasite Schistosoma mansoni Reveals Developmental Expression Profiles of Functional and Antigenic Glycan Motifs","volume":"14","author":"Smit","year":"2015","journal-title":"Mol. Cell. Proteom."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Hulme, B.J., Geyer, K.K., Forde-Thomas, J.E., Padalino, G., Phillips, D.W., Ittiprasert, W., Karinshak, S.E., Mann, V.H., Chalmers, I.W., and Brindley, P.J. (2022). Schistosoma mansoni \u03b1-N-Acetylgalactosaminidase (SmNAGAL) Regulates Coordinated Parasite Movement and Egg Production. PLoS Pathog., 18.","DOI":"10.1371\/journal.ppat.1009828"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"178","DOI":"10.12688\/wellcomeopenres.16031.1","article-title":"Large CRISPR-Cas-Induced Deletions in the Oxamniquine Resistance Locus of the Human Parasite Schistosoma mansoni","volume":"5","author":"Sankaranarayanan","year":"2020","journal-title":"Wellcome Open Res."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Ittiprasert, W., Chatupheeraphat, C., Mann, V.H., Li, W., Miller, A., Ogunbayo, T., Tran, K., Alrefaei, Y.N., Mentink-Kane, M., and Brindley, P.J. (2022). RNA-Guided AsCas12a- and SpCas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, Schistosoma mansoni. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23020631"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1038\/nbt.2623","article-title":"High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells","volume":"31","author":"Fu","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"100154","DOI":"10.1016\/j.crmeth.2021.100154","article-title":"Discovery and Validation of Human Genomic Safe Harbor Sites for Gene and Cell Therapies","volume":"2","author":"Aznauryan","year":"2022","journal-title":"Cell Rep. Methods"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"100535","DOI":"10.1016\/j.crmeth.2023.100535","article-title":"Targeted Insertion and Reporter Transgene Activity at a Gene Safe Harbor of the Human Blood Fluke, Schistosoma mansoni","volume":"3","author":"Ittiprasert","year":"2023","journal-title":"Cell Rep. Methods"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Rinaldi, G., Eckert, S.E., Tsai, I.J., Suttiprapa, S., Kines, K.J., Tort, J.F., Mann, V.H., Turner, D.J., Berriman, M., and Brindley, P.J. (2012). Germline Transgenesis and Insertional Mutagenesis in Schistosoma mansoni Mediated by Murine Leukemia Virus. PLoS Pathog., 8.","DOI":"10.1371\/journal.ppat.1002820"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/nature17664","article-title":"Efficient Introduction of Specific Homozygous and Heterozygous Mutations Using CRISPR\/Cas9","volume":"533","author":"Paquet","year":"2016","journal-title":"Nature"}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/13\/1\/48\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:48:08Z","timestamp":1760104088000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/13\/1\/48"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,16]]},"references-count":86,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,1]]}},"alternative-id":["biology13010048"],"URL":"https:\/\/doi.org\/10.3390\/biology13010048","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,16]]}}}