{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T13:54:50Z","timestamp":1772891690857,"version":"3.50.1"},"reference-count":63,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T00:00:00Z","timestamp":1712188800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Society for Promotion of Science","award":["19KK0176"],"award-info":[{"award-number":["19KK0176"]}]},{"name":"Japan Society for Promotion of Science","award":["F23-31"],"award-info":[{"award-number":["F23-31"]}]},{"name":"Japan Society for Promotion of Science","award":["2022"],"award-info":[{"award-number":["2022"]}]},{"name":"Environmental Radioactivity Research Network Center of Hirosaki","award":["19KK0176"],"award-info":[{"award-number":["19KK0176"]}]},{"name":"Environmental Radioactivity Research Network Center of Hirosaki","award":["F23-31"],"award-info":[{"award-number":["F23-31"]}]},{"name":"Environmental Radioactivity Research Network Center of Hirosaki","award":["2022"],"award-info":[{"award-number":["2022"]}]},{"name":"Tsukuba University","award":["19KK0176"],"award-info":[{"award-number":["19KK0176"]}]},{"name":"Tsukuba University","award":["F23-31"],"award-info":[{"award-number":["F23-31"]}]},{"name":"Tsukuba University","award":["2022"],"award-info":[{"award-number":["2022"]}]},{"name":"University of L\u2019Aquila","award":["19KK0176"],"award-info":[{"award-number":["19KK0176"]}]},{"name":"University of L\u2019Aquila","award":["F23-31"],"award-info":[{"award-number":["F23-31"]}]},{"name":"University of L\u2019Aquila","award":["2022"],"award-info":[{"award-number":["2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Since the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, great attention has been paid to the impact of chronic low-dose-rate (LDR) radiation exposure on biological systems. The reproductive system is sensitive to radiation, with implications connected to infertility. We investigated the testis ultrastructure of the wild large Japanese field mouse (Apodemus speciosus) from three areas contaminated after the FDNPP accident, with different levels of LDR radiation (0.29 \u00b5Sv\/h, 5.11 \u00b5Sv\/h, and 11.80 \u00b5Sv\/h). Results showed good preservation of the seminiferous tubules, comparable to the unexposed animals (controls), except for some ultrastructural modifications. Increases in the numerical density of lipid droplet clusters in spermatogenic cells were found at high levels of LDR radiation, indicating an antioxidant activity rising due to radiation recovery. In all groups, wide intercellular spaces were found between spermatogenic cells, and cytoplasmic vacuolization increased at intermediate and high levels and vacuolated mitochondria at the high-level. However, these findings were also related to the physiological dynamics of spermatogenesis. In conclusion, the testes of A. speciosus exposed to LDR radiation associated with the FDNPP accident showed a normal spermatogenesis, with some ultrastructural changes. These outcomes may add information on the reproductive potential of mammals chronically exposed to LDR radiation.<\/jats:p>","DOI":"10.3390\/biology13040239","type":"journal-article","created":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T02:25:32Z","timestamp":1712283932000},"page":"239","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Ultrastructural Analysis of Large Japanese Field Mouse (Apodemus speciosus) Testes Exposed to Low-Dose-Rate (LDR) Radiation after the Fukushima Nuclear Power Plant Accident"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3596-4075","authenticated-orcid":false,"given":"Marta","family":"Gatti","sequence":"first","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Sapienza University, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9183-6206","authenticated-orcid":false,"given":"Manuel","family":"Belli","sequence":"additional","affiliation":[{"name":"Department of Human Sciences and Promotion of the Quality of Life, San Raffaele Roma Open University, 00166 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariacarla","family":"De Rubeis","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Sapienza University, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syun","family":"Tokita","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Technology, Niigata University, Niigata 959-2181, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hikari","family":"Ikema","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Technology, Niigata University, Niigata 959-2181, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Yamashiro","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Technology, Niigata University, Niigata 959-2181, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohei","family":"Fujishima","sequence":"additional","affiliation":[{"name":"Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine, Hirosaki University, Aomori 036-8564, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6716-4059","authenticated-orcid":false,"given":"Donovan","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine, Hirosaki University, Aomori 036-8564, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0016-609X","authenticated-orcid":false,"given":"Valerie Swee Ting","family":"Goh","sequence":"additional","affiliation":[{"name":"Department of Radiobiology, Singapore Nuclear Research and Safety Initiative, National University of Singapore, Singapore 138602, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9207-3877","authenticated-orcid":false,"given":"Hisashi","family":"Shinoda","sequence":"additional","affiliation":[{"name":"Graduate School of Dentistry, Tohoku University, Sendai 980-8575, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8728-1655","authenticated-orcid":false,"given":"Akifumi","family":"Nakata","sequence":"additional","affiliation":[{"name":"Department of Life Science, Faculty of Pharmaceutical Sciences, Hokkaido University of Science, Hokkaido 006-8585, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manabu","family":"Fukumoto","sequence":"additional","affiliation":[{"name":"RIKEN Center for Advanced Intelligence Project, Pathology Informatics Team, Tokyo 103-0027, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3104-2134","authenticated-orcid":false,"given":"Tomisato","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine, Hirosaki University, Aomori 036-8564, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4626-6328","authenticated-orcid":false,"given":"Stefania Annarita","family":"Nottola","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Sapienza University, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9182-0586","authenticated-orcid":false,"given":"Guido","family":"Macchiarelli","sequence":"additional","affiliation":[{"name":"Department of Life, Health and Environmental Sciences, University of L\u2019Aquila, 67100 L\u2019Aquila, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3985-0112","authenticated-orcid":false,"given":"Maria Grazia","family":"Palmerini","sequence":"additional","affiliation":[{"name":"Department of Life, Health and Environmental Sciences, University of L\u2019Aquila, 67100 L\u2019Aquila, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"19526","DOI":"10.1073\/pnas.1111724108","article-title":"Assessment of individual radionuclide distributions from the Fukushima 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