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Metformin, the main pharmacologic treatment for pediatric type 2 diabetes, is administered to children during SC maturation. The present study aimed to analyze whether metformin affects SC energy metabolism and blood\u2013testis barrier (BTB) integrity. Primary SC cultures were used for the in vitro studies. In vivo effects were studied in Sprague\u2013Dawley rats treated with 200 mg\/kg metformin from Pnd14 to Pnd30. Metformin decreased fatty acid oxidation and increased 3-hydroxybutyrate production in vitro. Moreover, it decreased the transepithelial electrical resistance across the monolayer and induced ZO-1 redistribution, suggesting an alteration of cell junctions. In vivo, a mild but significant increase in BTB permeability and ZO-1 expression was observed in the metformin group, without changes in testicular histology and meiosis progression. Additionally, adult rats that received metformin treatment during the juvenile period showed no alteration in BTB permeability or daily sperm production. In conclusion, metformin exposure may affect BTB permeability in juvenile rats, but this seems not to influence spermatogenesis progression. Considering the results obtained in adult animals, it is possible to speculate that metformin treatment during the juvenile period does not affect testicular function in adulthood.<\/jats:p>","DOI":"10.3390\/biology13050330","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T03:21:04Z","timestamp":1715311264000},"page":"330","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effect of Metformin on Sertoli Cell Fatty Acid Metabolism and Blood\u2013Testis Barrier Formation"],"prefix":"10.3390","volume":"13","author":[{"given":"Gustavo Marcelo","family":"Rindone","sequence":"first","affiliation":[{"name":"Centro de Investigaciones Endocrinol\u00f3gicas \u201cDr. C\u00e9sar Bergad\u00e1\u201d (CEDIE), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), FEI\u2013Divisi\u00f3n de Endocrinolog\u00eda, Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez, Buenos Aires C1425EFD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Ercilia","family":"Dasso","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Endocrinol\u00f3gicas \u201cDr. C\u00e9sar Bergad\u00e1\u201d (CEDIE), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), FEI\u2013Divisi\u00f3n de Endocrinolog\u00eda, Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez, Buenos Aires C1425EFD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6833-2413","authenticated-orcid":false,"given":"Cecilia Lucia","family":"Centola","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Endocrinol\u00f3gicas \u201cDr. C\u00e9sar Bergad\u00e1\u201d (CEDIE), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), FEI\u2013Divisi\u00f3n de Endocrinolog\u00eda, Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez, Buenos Aires C1425EFD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristian Marcelo","family":"Sobarzo","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas (INBIOMED), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Facultad de Medicina, Universidad de Buenos Aires (UBA), Buenos Aires C1121ABG, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2678-622X","authenticated-orcid":false,"given":"Mar\u00eda Noel","family":"Galardo","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Endocrinol\u00f3gicas \u201cDr. C\u00e9sar Bergad\u00e1\u201d (CEDIE), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), FEI\u2013Divisi\u00f3n de Endocrinolog\u00eda, Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez, Buenos Aires C1425EFD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0313-7075","authenticated-orcid":false,"given":"Silvina Beatriz","family":"Meroni","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Endocrinol\u00f3gicas \u201cDr. C\u00e9sar Bergad\u00e1\u201d (CEDIE), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), FEI\u2013Divisi\u00f3n de Endocrinolog\u00eda, Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez, Buenos Aires C1425EFD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Fernanda","family":"Riera","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Endocrinol\u00f3gicas \u201cDr. C\u00e9sar Bergad\u00e1\u201d (CEDIE), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), FEI\u2013Divisi\u00f3n de Endocrinolog\u00eda, Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez, Buenos Aires C1425EFD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.semcdb.2016.06.018","article-title":"Regulation of the blood-testis barrier","volume":"59","author":"Stanton","year":"2016","journal-title":"Semin. 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