{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T13:50:57Z","timestamp":1770558657004,"version":"3.49.0"},"reference-count":34,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T00:00:00Z","timestamp":1723075200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>The impact of cardiorespiratory fitness (VO2max) and obesity on indices of oxidative stress in plasma and circulating exosome-like extracellular vesicles (ELVs) were examined following acute exercise. Indices of oxidative stress in plasma and isolated plasma ELVs were examined in aerobically trained (NW-Tr; n = 15) and untrained (NW-UTr; n = 18) normal-weight individuals and aerobically untrained individuals with obesity (Ob-Utr; n = 10) prior to and immediately following acute maximal treadmill running. Following exercise, ELV flotillin-1 expression (p = 0.008) and plasma total antioxidant capacity (TAC; p = 0.010) increased more in NW-UTr compared to NW-Tr and Ob-UTr participants, whereas plasma protein carbonyls (PC) decreased more in Ob-UTr compared to NW-Tr and NW-UTr groups. ELV glutathione (GSH) concentrations decreased more in NW-Tr compared to NW-UTr and Ob-UTr participants (p = 0.009), whereas lipid peroxidase (LPO) concentrations increased more in Ob-UTr compared to NW-Tr and NW-UTr participants (p = 0.003). Body mass index (BMI) was associated negatively with plasma TAC and PC (p &lt; 0.05) and positively with ELV LPO concentration responses (p = 0.009). Finally, plasma-to-total (plasma + ELV) GSH ratios decreased in Ob-UTr compared to NW-Tr and NW-UTr participants (p = 0.006), PC ratios increased in NW-Tr and NW-UTr compared to Ob-UTr subjects (p = 0.008), and reactive oxygen\/nitrogen species ratios increased in NW-UTr and decreased in Ob-UTr participants (p &lt; 0.001). BMI, independently of VO2max, differentially regulates indices of oxidative stress within plasma and circulating ELVs prior to and immediately following acute maximal treadmill exercise.<\/jats:p>","DOI":"10.3390\/biology13080599","type":"journal-article","created":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T12:13:14Z","timestamp":1723119194000},"page":"599","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of BMI and Cardiorespiratory Fitness on Oxidative Stress in Plasma and Circulating Exosomes Following Acute Exercise"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2082-4827","authenticated-orcid":false,"given":"Aaron L.","family":"Slusher","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Yale School of Medicine, New Haven, CT 06519, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nishant P.","family":"Visavadiya","sequence":"additional","affiliation":[{"name":"Exercise Biochemistry Laboratory, Department of Exercise Science and Health Promotion, Florida Atlantic University, FACSM 777 Glades Road, FH11A-126B, Boca Raton, FL 33431, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7874-1464","authenticated-orcid":false,"given":"Brandon G.","family":"Fico","sequence":"additional","affiliation":[{"name":"Exercise Biochemistry Laboratory, Department of Exercise Science and Health Promotion, Florida Atlantic University, FACSM 777 Glades Road, FH11A-126B, Boca Raton, FL 33431, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5034-9508","authenticated-orcid":false,"given":"Brisamar","family":"Est\u00e9banez","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine (BIOMED), University of Le\u00f3n, 24071 Le\u00f3n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6616-9234","authenticated-orcid":false,"given":"Edmund O.","family":"Acevedo","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Health Sciences, Virginia Commonwealth University, Richmond, VA 23220, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0425-8311","authenticated-orcid":false,"given":"Chun-Jung","family":"Huang","sequence":"additional","affiliation":[{"name":"Exercise Biochemistry Laboratory, Department of Exercise Science and Health Promotion, Florida Atlantic University, FACSM 777 Glades Road, FH11A-126B, Boca Raton, FL 33431, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1249\/JSR.0000000000000168","article-title":"Is exercise really medicine?: An evolutionary perspective","volume":"14","author":"Lieberman","year":"2015","journal-title":"Curr. Sports Med. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"n365","DOI":"10.1136\/bmj.n365","article-title":"Trends in obesity and adiposity measures by race or ethnicity among adults in the United States 2011\u201318: Population based study","volume":"372","author":"Liu","year":"2021","journal-title":"BMJ"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1007\/s10654-020-00650-3","article-title":"Global, regional and time-trend prevalence of central obesity: A systematic review and meta-analysis of 13.2 million subjects","volume":"35","author":"Wong","year":"2020","journal-title":"Eur. J. Epidemiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5937\/jomb0-24652","article-title":"The role of oxidative stress in the development of obesity and obesity-related metabolic disorders","volume":"40","author":"Pap","year":"2021","journal-title":"J. Med. Biochem."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Lea, A.J., Clark, A.G., Dahl, A.W., Devinsky, O., Garcia, A.R., Golden, C.D., Kamau, J., Kraft, T.S., Lim, Y.A.L., and Martins, D.J. (2023). Applying an evolutionary mismatch framework to understand disease susceptibility. PLoS Biol., 21.","DOI":"10.1371\/journal.pbio.3002311"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Zhou, Y., Li, H., and Xia, N. (2021). The interplay between adipose tissue and vasculature: Role of oxidative stress in obesity. Front. Cardiovasc. Med., 8.","DOI":"10.3389\/fcvm.2021.650214"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1055\/s-0043-119882","article-title":"Lifelong voluntary exercise modulates age-related changes in oxidative stress","volume":"39","author":"Bouzid","year":"2018","journal-title":"Int. J. Sports Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1186\/s12966-020-01007-5","article-title":"Dose-response relationship between physical activity and mortality in adults with noncommunicable diseases: A systematic review and meta-analysis of prospective observational studies","volume":"17","author":"Geidl","year":"2020","journal-title":"Int. J. Behav. Nutr. Phys. Act."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1161\/CIRCULATIONAHA.121.058162","article-title":"Long-term leisure-time physical activity intensity and all-cause and cause-specific mortality: A prospective cohort of US adults","volume":"146","author":"Lee","year":"2022","journal-title":"Circulation"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3336","DOI":"10.1002\/jcp.30094","article-title":"Effects of exercise on exosome release and cargo in in vivo and ex vivo models: A systematic review","volume":"236","author":"Huang","year":"2021","journal-title":"J. Cell Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s40798-015-0031-y","article-title":"Obesity-related oxidative stress: The impact of physical activity and diet manipulation","volume":"1","author":"Huang","year":"2015","journal-title":"Sports Med. Open"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"100019","DOI":"10.1016\/j.ocarto.2019.100019","article-title":"The secretome of skeletal muscle cells: A systematic review","volume":"2","author":"Florin","year":"2020","journal-title":"Osteoarthr. Cartil. Open"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.1249\/MSS.0000000000001672","article-title":"Moderate exercise enhances endothelial progenitor cell exosomes release and function","volume":"50","author":"Ma","year":"2018","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Nederveen, J.P., Warnier, G., Di Carlo, A., Nilsson, M.I., and Tarnopolsky, M.A. (2021). Extracellular vesicles and exosomes: Insights from exercise science. Front. Physiol., 11.","DOI":"10.3389\/fphys.2020.604274"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Lai, Z., Liang, J., Zhang, J., Mao, Y., Zheng, X., Shen, X., Lin, W., and Xu, G. (2023). Exosomes as a delivery tool of exercise-induced beneficial factors for the prevention and treatment of cardiovascular disease: A systematic review and meta-analysis. Front. Physiol., 14.","DOI":"10.3389\/fphys.2023.1190095"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1007\/s13105-023-00969-x","article-title":"Exercise-derived skeletal myogenic exosomes as mediators of intercellular crosstalk: A major player in health, disease, and exercise","volume":"79","author":"Ni","year":"2023","journal-title":"J. Physiol. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"a029827","DOI":"10.1101\/cshperspect.a029827","article-title":"Exosomes as mediators of the systemic adaptations to endurance exercise","volume":"8","author":"Safdar","year":"2018","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Vitucci, E., Martone, D., Alfieri, A., and Buono, P. (2023). Muscle-derived exosomes and exercise in cancer prevention. Front. Mol. Med., 3.","DOI":"10.3389\/fmmed.2023.1202190"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.cmet.2017.12.001","article-title":"Extracellular vesicles provide a means for tissue crosstalk during exercise","volume":"27","author":"Whitham","year":"2018","journal-title":"Cell Metab."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"28239","DOI":"10.3402\/jev.v4.28239","article-title":"Physical exercise induces rapid release of small extracellular vesicles into the circulation","volume":"4","author":"Helmig","year":"2015","journal-title":"J. Extracell. Vesicles"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Leuchtmann, A.B., Adak, V., Dilbaz, S., and Handschin, C. (2021). The role of the skeletal muscle secretome in mediating endurance and resistance training adaptations. Front. Physiol., 12.","DOI":"10.3389\/fphys.2021.709807"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1161\/CIRCRESAHA.118.314635","article-title":"Longterm exercise-derived exosomal miR-342-5p: A novel exerkine for cardioprotection","volume":"124","author":"Hou","year":"2019","journal-title":"Circ. Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Chiaradia, E., Tancini, B., Emiliani, C., Delo, F., Pellegrino, R.M., Tognoloni, A., Urbanelli, L., and Buratta, S. (2021). Extracellular vesicles under oxidative stress conditions: Biological properties and physiological roles. Cells, 10.","DOI":"10.3390\/cells10071763"},{"key":"ref_24","first-page":"ra60","article-title":"Toll-like receptor 4 senses oxidative stress mediated by the oxidation of phospholipids in extracellular vesicles","volume":"8","author":"Smole","year":"2015","journal-title":"Sci. Signal"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1038\/s41366-019-0460-7","article-title":"Effects of an acute bout of exercise on circulating extracellular vesicles: Tissue-, sex-, and BMI-related differences","volume":"44","author":"Rigamonti","year":"2020","journal-title":"Int. J. Obes."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"372","DOI":"10.3389\/fphys.2019.00372","article-title":"A short-term high-fat diet alters glutathione levels and IL-6 gene expression in oxidative skeletal muscles of young rats","volume":"10","author":"Andrich","year":"2019","journal-title":"Front. Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1249\/01.MSS.0000078924.61453.FB","article-title":"International physical activity questionnaire: 12-country reliability and validity","volume":"35","author":"Craig","year":"2003","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Est\u00e9banez, B., Amaro-Gahete, F.J., Gil-Gonz\u00e1lez, C., Gonz\u00e1lez-Gallego, J., Cuevas, M.J., and Jim\u00e9nez-Pav\u00f3n, D. (2023). Influence of 12-week concurrent training on exosome cargo and its relationship with cardiometabolic health parameters in men with obesity. Nutrients, 15.","DOI":"10.3390\/nu15133069"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/nri3041","article-title":"The anti-inflammatory effects of exercise: Mechanisms and implications for the prevention and treatment of disease","volume":"11","author":"Gleeson","year":"2011","journal-title":"Nat. Rev. Immunol."},{"key":"ref_30","first-page":"635","article-title":"GSH levels affect weight loss in individuals with metabolic syndrome and obesity following dietary therapy","volume":"16","author":"Goutzourelas","year":"2018","journal-title":"Exp. Ther. Med."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Lagman, M., Ly, J., Saing, T., Singh, M.K., Tudela, E.V., Morris, D., Chi, P.-T., Ochoa, C., Sathananthan, A., and Venketaraman, V. (2015). Investigating the causes for decreased levels of glutathione in individuals with type II diabetes. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0118436"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Lei, L.M., Lin, X., Xu, F., Shan, S.K., Guo, B., Li, F.X., Zheng, M.H., Wang, Y., Xu, Q.S., and Yuan, L.Q. (2021). Exosomes and obesity-related insulin resistance. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.651996"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1007\/s00421-018-3882-3","article-title":"Aerobic fitness alters the capacity of mononuclear cells to produce pentraxin 3 following maximal exercise","volume":"118","author":"Slusher","year":"2018","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1249\/MSS.0000000000001480","article-title":"Maximal exercise alters the inflammatory phenotype and response of mononuclear cells","volume":"50","author":"Slusher","year":"2018","journal-title":"Med. Sci. Sports Exerc."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/13\/8\/599\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:32:03Z","timestamp":1760110323000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/13\/8\/599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,8]]},"references-count":34,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["biology13080599"],"URL":"https:\/\/doi.org\/10.3390\/biology13080599","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,8]]}}}