{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:23:47Z","timestamp":1787066627215,"version":"build-2736575974"},"reference-count":49,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,22]],"date-time":"2024-09-22T00:00:00Z","timestamp":1726963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy"},{"name":"University of Palermo, Italy"},{"name":"Institute of Marine and Environmental Technology (IMET), Baltimore, MD, USA"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Background: The incidence of various types of cancer, for example, papillary thyroid carcinoma (PTC), is on the rise. Since therapeutic success depends greatly on early diagnosis, reliable diagnostic biomarkers must be identified, and easy-to-apply tools for detecting them must urgently be standardized. Here, we contribute to solving this medical challenge by assessing miRNAs suspected of promoting carcinogenesis in extracellular vesicles (EVs) that can be routinely obtained via liquid biopsy. We profit from current progress in cancerology that provides innovations in liquid biopsy and EVs analysis, along with the identification of miRNAs and chaperone system (CS) components implicated in carcinogenesis. Methods: We measured in EVs obtained from circulating blood plasma from PTC patients the levels of three miRNAs implicated in thyroid cancer, hsa-miR-1-3p, hsa-miR-206, and hsa-miR-221-3p, and most likely involved in the regulation of two members of the CS, Hsp60 and CCT. EVs were isolated from the plasma of patients with PTC and controls with benign goiter (BG) and from the culture medium of a PTC cell line (MDAT32) and were appropriately characterized. Results: The levels of miRNAs determined by RT-qPCR were consistently higher in PTC patients and decreased down to control levels after thyroidectomy. Bioinformatics showed that the miRNAs target genes are associated with the molecular pathogenesis of PTC. Conclusions: Our exploratory study reaffirms the potential in clinics of the selected miRNAs in EVs as useful biomarkers of PTC easily accessible via liquid biopsy, which is minimally invasive and amenable to periodic repetition, an improvement compared to the established fine-needle aspirate biopsy.<\/jats:p>","DOI":"10.3390\/biology13090743","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T08:56:06Z","timestamp":1727168166000},"page":"743","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["MiRNAs in Extracellular Vesicles as Biomarkers in Plasma of Papillary Thyroid Cancer Patients: A Proof-of-Concept Study"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2131-563X","authenticated-orcid":false,"given":"Giuseppa","family":"D\u2019Amico","sequence":"first","affiliation":[{"name":"Section of Human Anatomy, Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radha","family":"Santonocito","sequence":"additional","affiliation":[{"name":"Section of Human Anatomy, Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4502-8237","authenticated-orcid":false,"given":"Godfrey","family":"Grech","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine and Surgery, University of Malta, MSD 2080 Msida, Malta"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppa","family":"Graceffa","sequence":"additional","affiliation":[{"name":"Department of Precision Medicine in the Medical, Surgical and Critical Area, University of Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4396-3563","authenticated-orcid":false,"given":"Calogero","family":"Cipolla","sequence":"additional","affiliation":[{"name":"Department of Precision Medicine in the Medical, Surgical and Critical Area, University of Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Scalia","sequence":"additional","affiliation":[{"name":"Section of Human Anatomy, Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7775-5928","authenticated-orcid":false,"given":"Samuele","family":"Raccosta","sequence":"additional","affiliation":[{"name":"Cell-Tech Hub, Institute of Biophysics, National Research Council of Italy, 90146 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9843-0428","authenticated-orcid":false,"given":"Mauro","family":"Manno","sequence":"additional","affiliation":[{"name":"Cell-Tech Hub, Institute of Biophysics, National Research Council of Italy, 90146 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9689-8844","authenticated-orcid":false,"given":"Everly","family":"Conway de Macario","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore-Institute of Marine and Environmental Technology (IMET), Baltimore, MD 21202, USA"},{"name":"Euro-Mediterranean Institute of Science and Technology (IEMEST), 90139 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6181-2876","authenticated-orcid":false,"given":"Alberto J. L.","family":"Macario","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore-Institute of Marine and Environmental Technology (IMET), Baltimore, MD 21202, USA"},{"name":"Euro-Mediterranean Institute of Science and Technology (IEMEST), 90139 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9288-1148","authenticated-orcid":false,"given":"Francesco","family":"Cappello","sequence":"additional","affiliation":[{"name":"Section of Human Anatomy, Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, Italy"},{"name":"Euro-Mediterranean Institute of Science and Technology (IEMEST), 90139 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6610-5268","authenticated-orcid":false,"given":"Francesca","family":"Rappa","sequence":"additional","affiliation":[{"name":"Section of Human Anatomy, Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, Italy"},{"name":"The Institute of Translational Pharmacology, National Research Council of Italy (CNR), 90146 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8260-8471","authenticated-orcid":false,"given":"Celeste","family":"Caruso Bavisotto","sequence":"additional","affiliation":[{"name":"Section of Human Anatomy, Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, Italy"},{"name":"Euro-Mediterranean Institute of Science and Technology (IEMEST), 90139 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Campanella","sequence":"additional","affiliation":[{"name":"Section of Human Anatomy, Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Goodarzi, E., Moslem, A., Feizhadad, H., Jarrahi, A., Adineh, H., Sohrabivafa, M., and Khazaei, Z. (2019). Epidemiology, Incidence and Mortality of Thyroid Cancer and Their Relationship with the Human Development Index in the World: An Ecology Study in 2018. Adv. Hum. Biol., 9.","DOI":"10.4103\/AIHB.AIHB_2_19"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Romano, C., Martorana, F., Pennisi, M.S., Stella, S., Massimino, M., Tirr\u00f2, E., Vitale, S.R., Di Gregorio, S., Puma, A., and Tomarchio, C. (2021). Opportunities and Challenges of Liquid Biopsy in Thyroid Cancer. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22147707"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e12404","DOI":"10.1002\/jev2.12404","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Alberti, G., S\u00e1nchez-L\u00f3pez, C.M., Andres, A., Santonocito, R., Campanella, C., Cappello, F., and Marcilla, A. (2021). Molecular Profile Study of Extracellular Vesicles for the Identification of Useful Small \u201cHit\u201d in Cancer Diagnosis. Appl. Sci., 11.","DOI":"10.3390\/app112210787"},{"key":"ref_5","first-page":"136","article-title":"Exosomes: Can Doctors Still Ignore Their Existence?","volume":"8","author":"Rappa","year":"2013","journal-title":"EuroMediterranean Biomed. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2265","DOI":"10.1038\/s41418-023-01217-x","article-title":"METTL3 Inhibition Induced by M2 Macrophage-Derived Extracellular Vesicles Drives Anti-PD-1 Therapy Resistance via M6A-CD70-Mediated Immune Suppression in Thyroid Cancer","volume":"30","author":"Ning","year":"2023","journal-title":"Cell Death Differ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1530\/ERC-21-0343","article-title":"Two MiRNAs Enriched in Plasma Extracellular Vesicles Are Potential Biomarkers for Thyroid Cancer","volume":"29","author":"Delcorte","year":"2022","journal-title":"Endocr. Relat. Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Mardente, S., Aventaggiato, M., Splendiani, E., Mari, E., Zicari, A., Catanzaro, G., Po, A., Coppola, L., and Tafani, M. (2023). Extra-Cellular Vesicles Derived from Thyroid Cancer Cells Promote the Epithelial to Mesenchymal Transition (EMT) and the Transfer of Malignant Phenotypes through Immune Mediated Mechanisms. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24032754"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.semcancer.2021.05.029","article-title":"Chaperonins in Cancer: Expression, Function, and Migration in Extracellular Vesicles","volume":"86","author":"Macario","year":"2022","journal-title":"Semin. Cancer Biol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Paladino, L., Vitale, A., Santonocito, R., Pitruzzella, A., Cipolla, C., Graceffa, G., Bucchieri, F., Conway de Macario, E., Macario, A.J.L., and Rappa, F. (2021). Molecular Chaperones and Thyroid Cancer. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22084196"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.semcancer.2021.09.010","article-title":"Extracellular Heat Shock Proteins in Cancer: From Early Diagnosis to New Therapeutic Approach","volume":"86","author":"Campanella","year":"2022","journal-title":"Semin. Cancer Biol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Caruso Bavisotto, C., Marino Gammazza, A., Lo Cascio, F., Mocciaro, E., Vitale, A.M., Vergilio, G., Pace, A., Cappello, F., Campanella, C., and Palumbo Piccionello, A. (2020). Curcumin Affects HSP60 Folding Activity and Levels in Neuroblastoma Cells. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21020661"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Graziano, F., Iacopino, D.G., Cammarata, G., Scalia, G., Campanella, C., Giannone, A.G., Porcasi, R., Florena, A.M., Conway de Macario, E., and Macario, A.J.L. (2021). The Triad Hsp60-MiRNAs-Extracellular Vesicles in Brain Tumors: Assessing Its Components for Understanding Tumorigenesis and Monitoring Patients. Appl. Sci., 11.","DOI":"10.3390\/app11062867"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"D\u2019Amico, G., Santonocito, R., Vitale, A.M., Scalia, F., Marino Gammazza, A., Campanella, C., Bucchieri, F., Cappello, F., and Caruso Bavisotto, C. (2023). Air Pollution: Role of Extracellular Vesicles-Derived Non-Coding RNAs in Environmental Stress Response. Cells, 12.","DOI":"10.3390\/cells12111498"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.7150\/jca.18026","article-title":"Isolation and Identification of MiRNAs in Exosomes Derived from Serum of Colon Cancer Patients","volume":"8","author":"Zhao","year":"2017","journal-title":"J. Cancer"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"109995","DOI":"10.1016\/j.cellsig.2021.109995","article-title":"Micro-RNA: The Darkhorse of Cancer","volume":"83","author":"Budakoti","year":"2021","journal-title":"Cell Signal."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1210\/jc.2011-3059","article-title":"Circulating MicroRNA Profiles as Potential Biomarkers for Diagnosis of Papillary Thyroid Carcinoma","volume":"97","author":"Yu","year":"2012","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1210\/clinem\/dgac009","article-title":"Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence","volume":"107","author":"Robledo","year":"2022","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3592","DOI":"10.1016\/j.febslet.2010.07.027","article-title":"MiR-1\/MiR-206 Regulate Hsp60 Expression Contributing to Glucose-Mediated Apoptosis in Cardiomyocytes","volume":"584","author":"Shan","year":"2010","journal-title":"FEBS Lett."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Caruso Bavisotto, C., Cipolla, C., Graceffa, G., Barone, R., Bucchieri, F., Bulone, D., Cabibi, D., Campanella, C., Marino Gammazza, A., and Pitruzzella, A. (2019). Immunomorphological Pattern of Molecular Chaperones in Normal and Pathological Thyroid Tissues and Circulating Exosomes: Potential Use in Clinics. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20184496"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Paladino, L., Santonocito, R., Graceffa, G., Cipolla, C., Pitruzzella, A., Cabibi, D., Cappello, F., Conway de Macario, E., Macario, A.J.L., and Bucchieri, F. (2023). Immunomorphological Patterns of Chaperone System Components in Rare Thyroid Tumors with Promise as Biomarkers for Differential Diagnosis and Providing Clues on Molecular Mechanisms of Carcinogenesis. Cancers, 15.","DOI":"10.3390\/cancers15082403"},{"key":"ref_22","first-page":"4357","article-title":"Differential Protein Expression of Lymph Node Metastases of Papillary Thyroid Carcinoma Harboring the BRAF Mutation","volume":"33","author":"Park","year":"2013","journal-title":"Anticancer. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"652","DOI":"10.3892\/etm.2021.10084","article-title":"MiR-221-3p and MiR-222-3p Regulate the SOCS3\/STAT3 Signaling Pathway to Downregulate the Expression of NIS and Reduce Radiosensitivity in Thyroid Cancer","volume":"21","author":"Ye","year":"2021","journal-title":"Exp. Ther. Med."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Cai, S., Ma, J., Wang, Y., Cai, Y., Xie, L., Chen, X., Yang, Y., and Peng, Q. (2021). Biomarker Value of MiR-221 and MiR-222 as Potential Substrates in the Differential Diagnosis of Papillary Thyroid Cancer Based on Data Synthesis and Bioinformatics Approach. Front. Endocrinol., 12.","DOI":"10.3389\/fendo.2021.794490"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12957-016-1086-z","article-title":"MiR-221, a Potential Prognostic Biomarker for Recurrence in Papillary Thyroid Cancer","volume":"15","author":"Dai","year":"2017","journal-title":"World J. Surg. Oncol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/s12033-022-00473-5","article-title":"MiR-221-3p Facilitates Thyroid Cancer Cell Proliferation and Inhibit Apoptosis by Targeting FOXP2 Through Hedgehog Pathway","volume":"64","author":"Chang","year":"2022","journal-title":"Mol. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Santonocito, R., Paladino, L., Vitale, A.M., D\u2019Amico, G., Zummo, F.P., Pirrotta, P., Raccosta, S., Manno, M., Accomando, S., and D\u2019Arpa, F. (2024). Nanovesicular Mediation of the Gut\u2013Brain Axis by Probiotics: Insights into Irritable Bowel Syndrome. Biology, 13.","DOI":"10.3390\/biology13050296"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Paterna, A., Rao, E., Adamo, G., Raccosta, S., Picciotto, S., Romancino, D., Noto, R., Touzet, N., Bongiovanni, A., and Manno, M. (2022). Isolation of Extracellular Vesicles from Microalgae: A Renewable and Scalable Bioprocess. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.836747"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"12081","DOI":"10.1002\/jev2.12081","article-title":"Nanoalgosomes: Introducing Extracellular Vesicles Produced by Microalgae","volume":"10","author":"Adamo","year":"2021","journal-title":"J. Extracell. Vesicles"},{"key":"ref_30","unstructured":"Berne, B.J., and Pecora, R. (2000). Dynamic Light Scattering: With Applications to Chemistry, Biology, and Physics, Dover Publications."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.molcel.2007.06.017","article-title":"MicroRNA Targeting Specificity in Mammals: Determinants beyond Seed Pairing","volume":"27","author":"Grimson","year":"2007","journal-title":"Mol. Cell"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/S2213-8587(22)00035-3","article-title":"The Epidemiological Landscape of Thyroid Cancer Worldwide: GLOBOCAN Estimates for Incidence and Mortality Rates in 2020","volume":"10","author":"Pizzato","year":"2022","journal-title":"Lancet Diabetes Endocrinol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.2147\/OTT.S190364","article-title":"MicroRNA-221 Promotes Papillary Thyroid Carcinoma Cells Migration and Invasion via Targeting RECK and Regulating Epithelial\u2013Mesenchymal Transition","volume":"12","author":"Wei","year":"2019","journal-title":"Onco Targets Ther."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1111\/1440-1681.12457","article-title":"HSP60 Overexpression Increases the Protein Levels of the P110\u03b1 Subunit of Phosphoinositide 3-Kinase and c-Myc","volume":"42","author":"Yan","year":"2015","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"28849","DOI":"10.18632\/oncotarget.6680","article-title":"The Histone Deacetylase Inhibitor SAHA Induces HSP60 Nitration and Its Extracellular Release by Exosomal Vesicles in Human Lung-Derived Carcinoma Cells","volume":"7","author":"Campanella","year":"2016","journal-title":"Oncotarget"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Marino Gammazza, A., Campanella, C., Barone, R., Caruso Bavisotto, C., Gorska, M., Wozniak, M., Carini, F., Cappello, F., D\u2019Anneo, A., and Lauricella, M. (2017). Doxorubicin Anti-Tumor Mechanisms Include Hsp60 Post-Translational Modifications Leading to the Hsp60\/P53 Complex Dissociation and Instauration of Replicative Senescence. Cancer Lett., 385.","DOI":"10.1016\/j.canlet.2016.10.045"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1186\/s12903-023-03311-5","article-title":"High expression of HSP60 and survivin predicts poor prognosis for oral squamous cell carcinoma patients","volume":"23","author":"Zhou","year":"2023","journal-title":"BMC Oral Health"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s13048-023-01156-8","article-title":"Knockdown of heat shock protein family D member 1 (HSPD1) promotes proliferation and migration of ovarian cancer cells via disrupting the stability of mitochondrial 3-oxoacyl-ACP synthase (OXSM)","volume":"16","author":"Duan","year":"2023","journal-title":"J. Ovarian Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e05005","DOI":"10.7554\/eLife.05005","article-title":"Predicting effective microRNA target sites in mammalian mRNAs","volume":"12","author":"Agarwal","year":"2015","journal-title":"eLife"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/s41568-018-0002-y","article-title":"Known and Novel Roles of the MET Oncogene in Cancer: A Coherent Approach to Targeted Therapy","volume":"18","author":"Comoglio","year":"2018","journal-title":"Nat. Rev. Cancer"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1038\/s41416-022-01805-7","article-title":"HDAC4 Promotes the Growth and Metastasis of Gastric Cancer via Autophagic Degradation of MEKK3","volume":"127","author":"Zang","year":"2022","journal-title":"Br. J. Cancer"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.21873\/anticanres.13220","article-title":"Histone Deacetylase Inhibitors and Anaplastic Thyroid Carcinoma","volume":"39","author":"Spartalis","year":"2019","journal-title":"Anticancer Res."},{"key":"ref_44","first-page":"5237","article-title":"MicroRNA-409-3p Suppresses Cell Proliferation and Cell Cycle Progression by Targeting Cyclin D2 in Papillary Thyroid Carcinoma","volume":"16","author":"Zhao","year":"2018","journal-title":"Oncol. Lett."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5651","DOI":"10.1038\/onc.2008.178","article-title":"MiR-221 Controls CDKN1C\/P57 and CDKN1B\/P27 Expression in Human Hepatocellular Carcinoma","volume":"27","author":"Fornari","year":"2008","journal-title":"Oncogene"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e210352","DOI":"10.1530\/ERC-21-0352","article-title":"TAGLN2 Promotes Papillary Thyroid Carcinoma Invasion via the Rap1\/PI3K\/AKT Axis","volume":"30","author":"Wang","year":"2023","journal-title":"Endocr. Relat. Cancer"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Scalia, F., Marino Gammazza, A., Conway de Macario, E., Macario, A.J.L., and Cappello, F. (2019). Myelin Pathology: Involvement of Molecular Chaperones and the Promise of Chaperonotherapy. Brain Sci., 9.","DOI":"10.3390\/brainsci9110297"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1038\/s41416-022-01747-0","article-title":"CCT5 induces epithelial-mesenchymal transition to promote gastric cancer lymph node metastasis by activating the Wnt\/\u03b2-catenin signalling pathway","volume":"126","author":"Li","year":"2022","journal-title":"Br. J. Cancer"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Ali Syeda, Z., Langden, S.S.S., Munkhzul, C., Lee, M., and Song, S.J. (2020). Regulatory Mechanism of MicroRNA Expression in Cancer. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21051723"}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/13\/9\/743\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:01:59Z","timestamp":1760112119000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/13\/9\/743"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,22]]},"references-count":49,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["biology13090743"],"URL":"https:\/\/doi.org\/10.3390\/biology13090743","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,22]]}}}