{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:35:41Z","timestamp":1791999341591,"version":"4.4.0"},"reference-count":91,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["21-64-00019"],"award-info":[{"award-number":["21-64-00019"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>The search for the microorganisms responsible for sulfide formation and corrosion of steel equipment in the oil fields of Tatarstan (Russia) resulted in the isolation of a new halotolerant strictly anaerobic sulfate-reducing bacterium, strain 5S69T. The cells were motile curved Gram-negative rods. Optimal growth was observed in the presence of 2.0\u20134.0% (w\/v) NaCl, at pH 6.5, and at 23\u201328 \u00b0C under sulfate-reducing conditions. The isolate was capable of chemoorganotrophic growth with sulfate and other sulfoxides as electron acceptors, resulting in sulfide formation; and of pyruvate fermentation resulting in formation of H2 and acetate. The strain utilized lactate, pyruvate, ethanol, methanol, fumarate, and fructose, as well as H2\/CO2\/acetate for sulfate reduction. The genome size of the type strain 5S69T was 4.16 Mb with a G + C content of 63.0 mol%. On the basis of unique physiological properties and results of the 16S rRNA gene-based phylogenetic analysis, phylogenomic analysis of the 120 conserved single copy proteins and genomic indexes (ANI, AAI, and dDDH), assigning the type strain 5S69T ((VKM B-3653T = KCTC 25499T) to a new species within the genus Pseudodesulfovibrio, is suggested, with the proposed name Pseudodesulfovibrio methanolicus sp. nov. Genome analysis of the new isolate showed several genes involved in sulfate reduction and its sulfide-producing potential in oil fields with high saline formation water.<\/jats:p>","DOI":"10.3390\/biology13100800","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T08:35:23Z","timestamp":1728290123000},"page":"800","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Phenotypic and Genomic Characterization of a Sulfate-Reducing Bacterium Pseudodesulfovibrio methanolicus sp. nov. Isolated from a Petroleum Reservoir in Russia"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7599-114X","authenticated-orcid":false,"given":"Salimat K.","family":"Bidzhieva","sequence":"first","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4562-3522","authenticated-orcid":false,"given":"Tatyana P.","family":"Tourova","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitaly V.","family":"Kadnikov","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salima R.","family":"Samigullina","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2422-4423","authenticated-orcid":false,"given":"Diyana S.","family":"Sokolova","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey B.","family":"Poltaraus","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander N.","family":"Avtukh","sequence":"additional","affiliation":[{"name":"Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera M.","family":"Tereshina","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey V.","family":"Beletsky","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8245-8757","authenticated-orcid":false,"given":"Andrey V.","family":"Mardanov","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6586-2467","authenticated-orcid":false,"given":"Tamara N.","family":"Nazina","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1023\/A:1002434330514","article-title":"Microbiology of petroleum reservoirs","volume":"77","author":"Magot","year":"2000","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0065-2164(08)00806-X","article-title":"Microbial processes in oil fields: Culprits, problems and opportunities","volume":"66","author":"Youssef","year":"2009","journal-title":"Adv. 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