{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:34:40Z","timestamp":1787027680176,"version":"build-2736575974"},"reference-count":42,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jiangsu Revitalization of Seed Industry","award":["JBGS (2021) 031"],"award-info":[{"award-number":["JBGS (2021) 031"]}]},{"name":"Jiangsu Revitalization of Seed Industry","award":["2022YFD2400702"],"award-info":[{"award-number":["2022YFD2400702"]}]},{"name":"Jiangsu Revitalization of Seed Industry","award":["2023TD39"],"award-info":[{"award-number":["2023TD39"]}]},{"name":"Jiangsu Revitalization of Seed Industry","award":["2023JBFM05"],"award-info":[{"award-number":["2023JBFM05"]}]},{"name":"National Key R&amp;D Program of China on Eriocheir sinensis","award":["JBGS (2021) 031"],"award-info":[{"award-number":["JBGS (2021) 031"]}]},{"name":"National Key R&amp;D Program of China on Eriocheir sinensis","award":["2022YFD2400702"],"award-info":[{"award-number":["2022YFD2400702"]}]},{"name":"National Key R&amp;D Program of China on Eriocheir sinensis","award":["2023TD39"],"award-info":[{"award-number":["2023TD39"]}]},{"name":"National Key R&amp;D Program of China on Eriocheir sinensis","award":["2023JBFM05"],"award-info":[{"award-number":["2023JBFM05"]}]},{"name":"Freshwater Shrimp and Crab Genetic Breeding Innovation Team project","award":["JBGS (2021) 031"],"award-info":[{"award-number":["JBGS (2021) 031"]}]},{"name":"Freshwater Shrimp and Crab Genetic Breeding Innovation Team project","award":["2022YFD2400702"],"award-info":[{"award-number":["2022YFD2400702"]}]},{"name":"Freshwater Shrimp and Crab Genetic Breeding Innovation Team project","award":["2023TD39"],"award-info":[{"award-number":["2023TD39"]}]},{"name":"Freshwater Shrimp and Crab Genetic Breeding Innovation Team project","award":["2023JBFM05"],"award-info":[{"award-number":["2023JBFM05"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, Freshwater Fisheries Research Center, CAFS","award":["JBGS (2021) 031"],"award-info":[{"award-number":["JBGS (2021) 031"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, Freshwater Fisheries Research Center, CAFS","award":["2022YFD2400702"],"award-info":[{"award-number":["2022YFD2400702"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, Freshwater Fisheries Research Center, CAFS","award":["2023TD39"],"award-info":[{"award-number":["2023TD39"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund, Freshwater Fisheries Research Center, CAFS","award":["2023JBFM05"],"award-info":[{"award-number":["2023JBFM05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>(1) Background: Global climate change is intensifying, and the vigorous development and utilization of saline\u2013alkali land is of great significance. As an important economic aquatic species in the context of saline\u2013alkali aquaculture, it is highly significant to explore the regulatory mechanisms of Eriocheir sinensis under alkaline conditions. In particular, the brain (cerebral ganglion for crustaceans) serves as a vital regulatory organ in response to environmental stress; (2) Methods: In this study, a comparative transcriptome approach was employed to investigate the key regulatory genes and molecular regulatory mechanisms in the cerebral ganglion of E. sinensis under alkaline stress. (3) Results: The results demonstrated that the cerebral ganglion of E. sinensis exhibited a positive response to acute alkaline stress. Pathways associated with signal transduction and substance transportation, such as \u201cphagosome\u201d and \u201cregulation of actin cytoskeleton\u201d, along with regulatory genes involved in antioxidation, were upregulated synergistically to maintain homeostasis under alkaline stress. Furthermore, it was discovered for the first time that bursicon plays a positive regulatory role in the adaptation of E. sinensis to alkalinity. (4) Conclusions: The present study elucidates the molecular regulatory pattern of the cerebral ganglion in E. sinensis under acute alkaline stress as well as revealing a novel role of bursicon in facilitating adaptation to alkalinity in E. sinensis, providing valuable theoretical insights into the molecular regulatory mechanisms underlying the responses of cerebral ganglia to saline\u2013alkali environments. These findings also offer a theoretical reference for promoting the sustainable development of the E. sinensis breeding industry under saline\u2013alkali conditions.<\/jats:p>","DOI":"10.3390\/biology14010084","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T12:27:54Z","timestamp":1737030474000},"page":"84","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular Mechanisms Underlying Substance Transport, Signal Transduction, and Anti-Stress Regulation, as Well as Anti-Alkaline Regulation via Bursicon in the Cerebral Ganglion of Chinese Mitten Crab Eriocheir sinensis Under Alkaline Stress"],"prefix":"10.3390","volume":"14","author":[{"given":"Meiyao","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Wuxi 214081, China"},{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiachun","family":"Ge","sequence":"additional","affiliation":[{"name":"Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gangchun","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Wuxi 214081, China"},{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5643-2844","authenticated-orcid":false,"given":"Yongkai","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Wuxi 214081, China"},{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s00267-018-1117-3","article-title":"Global aquaculture productivity, environmental sustainability, and climate change adaptability","volume":"63","author":"Ahmed","year":"2019","journal-title":"Environ. Manag."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"110639","DOI":"10.1016\/j.ecolind.2023.110639","article-title":"Assessing a multi-method approach for dryland soil salinization with respect to climate change and global warming-The example of the Bajestan region (NE Iran)","volume":"154","author":"Khosravichenar","year":"2023","journal-title":"Ecol. Indic."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"eabq0110","DOI":"10.1126\/sciadv.abq0110","article-title":"North Atlantic surface ocean warming and salinization in response to middle Eocene greenhouse warming","volume":"9","author":"Cramwinckel","year":"2023","journal-title":"Sci. Adv."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"119354","DOI":"10.1016\/j.watres.2022.119354","article-title":"Climate-induced salinization may lead to increased lake nitrogen retention","volume":"228","author":"Jiang","year":"2023","journal-title":"Water Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e32922","DOI":"10.1016\/j.heliyon.2024.e32922","article-title":"Status and prospects of product processing and sustainable utilization of Chinese mitten crab (Eriocheir sinensis)","volume":"10","author":"Xu","year":"2024","journal-title":"Heliyon"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"122544","DOI":"10.1016\/j.envpol.2023.122544","article-title":"Comparative assessment of Chinese mitten crab aquaculture in China: Spatiotemporal changes and trade-offs","volume":"337","author":"Liu","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"741518","DOI":"10.1016\/j.aquaculture.2024.741518","article-title":"The Chinese mitten crab (Eriocheir sinensis) and its microbiome: A review","volume":"595","author":"Ghosh","year":"2025","journal-title":"Aquaculture"},{"key":"ref_8","first-page":"13","article-title":"Culture status and green culture technology of Chinese mitten crab (Eriocheir sinensis)","volume":"10","author":"Zhang","year":"2024","journal-title":"Sci. Farming"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1017\/S0025315417000169","article-title":"Understanding and forecasting dispersal of non-indigenous marine decapods (Crustacea: Decapoda) in East European and North Asian waters","volume":"97","author":"Spiridonov","year":"2017","journal-title":"J. Mar. Biol. Assoc. U. K."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"106481","DOI":"10.1016\/j.aquatox.2023.106481","article-title":"Alkali exposure induces autophagy through activation of the MAPKpathway by ROS and inhibition of mTOR in Eriocheir sinensis","volume":"258","author":"Wang","year":"2023","journal-title":"Aquat. Toxicol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhang, R., Zhao, Z., Li, M., Luo, L., Wang, S., Guo, K., and Xu, W. (2023). Metabolomics analysis reveals the response mechanism to carbonate alkalinity toxicity in the gills of Eriocheir sinensis. Comp. Biochem. Phys. C, 263.","DOI":"10.1016\/j.cbpc.2022.109487"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"740858","DOI":"10.1016\/j.aquaculture.2024.740858","article-title":"Comparative transcriptomic and epigenomic analyses to identify the cold resistance-associated genes and disclose the regulatory mechanisms in tilapias","volume":"587","author":"Song","year":"2024","journal-title":"Aquaculture"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1007\/s10695-024-01332-7","article-title":"Response of CRH system in brain and gill of marine medaka to seawater acidification","volume":"50","author":"Chen","year":"2024","journal-title":"Fish Physiol. Biochem."},{"key":"ref_14","first-page":"67","article-title":"Comparative transcriptology reveals effects of circadian rhythm in the nervous system on precocious puberty of the female Chinese mitten crab","volume":"29","author":"Fu","year":"2019","journal-title":"Comp. Biochem. Phys. D"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.ygeno.2019.10.014","article-title":"Molecular insights into the sex-differential regulation of signal transduction in the cerebral ganglion and metabolism in the hepatopancreas of Eriocheir sinensis during reproduction","volume":"112","author":"Wang","year":"2020","journal-title":"Genomics"},{"key":"ref_16","first-page":"36","article-title":"Study on toxicity of salinity and alkalinity on Eriocheir sinensis","volume":"53","author":"Yang","year":"2021","journal-title":"J. Northeast. Agric. Univ."},{"key":"ref_17","unstructured":"(2008). Industrial Sodium Bicarbonate Standard (Standard No. GBT1606-2008). (In Chinese)."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1093\/nar\/gkm882","article-title":"KEGG for linking genomes to life and the environment","volume":"36","author":"Kanehisa","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1038\/nmeth.2251","article-title":"Streaming fragment assignment for real-time analysis of sequencing experiments","volume":"10","author":"Roberts","year":"2013","journal-title":"Nat. Methods"},{"key":"ref_23","unstructured":"Anders, S., and Huber, W. (2013). Differential Expression of RNA Seq Data at the Gene Level the DESeq Package, EMBL."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.ygcen.2012.11.018","article-title":"Bursicon and neuropeptide cascades during the ecdysis program of the shore crab, Carcinus maenas","volume":"182","author":"Webster","year":"2013","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1111\/are.14599","article-title":"Alteration of the haemocyte parameters, expression of immune-related and neurohormone bursicon genes of giant river prawn Macrobrachium rosenbergii (de Man) in the response to salinity stress and ammonia stress","volume":"51","author":"Jariyapong","year":"2020","journal-title":"Aquac. Res."},{"key":"ref_26","unstructured":"Wang, Q., Li, H., Song, M., Yang, C., Zhao, Z., Zhao, X., Xu, W., Guo, W., and Xie, J. (2015). Pharmaceutical Cell Biology, China Medical Science Press."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.brainresbull.2016.06.004","article-title":"Tropomyosins in the healthy and diseased nervous system","volume":"126","author":"Brettle","year":"2016","journal-title":"Brain Res. Bull."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Jin, S., Zhou, R., Gao, X., Xiong, Y., Zhang, W., Qiao, H., Wu, Y., Jiang, S., and Fu, H. (2024). Identification of the effects of alkalinity exposure on the gills of oriental river prawns, Macrobrachium nipponense. BMC Genom., 25.","DOI":"10.1186\/s12864-024-10659-7"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"740175","DOI":"10.1016\/j.aquaculture.2023.740175","article-title":"Comprehensive analyses of annexins in naked carp (Gymnocypris przewalskii) unveil their roles in saline-alkaline stress","volume":"579","author":"Linlin","year":"2024","journal-title":"Aquaculture"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/s41556-018-0244-7","article-title":"The lysosome as a cellular centre for signalling, metabolism and quality control","volume":"21","author":"Lawrence","year":"2019","journal-title":"Nat. Cell Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/s41419-023-05786-0","article-title":"Cathepsin B in programmed cell death machinery: Mechanisms of execution and regulatory pathways","volume":"14","author":"Xie","year":"2023","journal-title":"Cell Death Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"9911","DOI":"10.1007\/s10499-024-01642-w","article-title":"Camel whey protein hydrolysate diet mitigates alkaline stress-induced biochemical disorders and restores the target of rapamycin, MAPK pathway, and autophagy-related gene expression in Nile tilapia","volume":"32","author":"Ibrahim","year":"2024","journal-title":"Aquacult. Int."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1146\/annurev.bi.60.070191.002025","article-title":"Structure and function of signal-transducing GTP-binding proteins","volume":"60","author":"Kaziro","year":"1991","journal-title":"Annu. Rev. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Qin, Z., Ge, Q.Q., Wang, J.J., Li, M.D., and Liu, P. (2021). Comparative transcriptomic and proteomic analysis of Exopalaemon carinicauda in response to alkalinity stress. Front. Mar. Sci., 8.","DOI":"10.3389\/fmars.2021.759923"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1007\/s00343-022-2271-2","article-title":"Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (Fc\u03b4GST) from Chinese shrimp Fenneropenaeus chinensis","volume":"41","author":"Li","year":"2023","journal-title":"J. Oceanol. Limnol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Xiao, M., Nan, Y., Yang, Y., Li, H., and Duan, Y. (2024). Changes in physiological homeostasis in the gills of Litopenaeus vannamei under carbonate alkalinity stress and recovery conditions. Fishes, 9.","DOI":"10.3390\/fishes9110463"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Xu, J., Li, Q., Xu, L., Wang, S., Jiang, Y., Zhao, Z., Zhang, Y., Li, J., Dong, C., and Xu, P. (2013). Gene expression changes leading extreme alkaline tolerance in Amur ide (Leuciscus waleckii) inhabiting soda lake. BMC Genom., 14.","DOI":"10.1186\/1471-2164-14-682"},{"key":"ref_38","first-page":"100790","article-title":"Effects of pH changes on blood physiology, antioxidant responses and Ig M of juvenile olive flounder, Paralichthys olivaceus","volume":"21","author":"Kim","year":"2021","journal-title":"Aquacult. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"109201","DOI":"10.1016\/j.fsi.2023.109201","article-title":"Glutathione peroxidase 4 knock-down triggers ferroptosis in Penaeus vannamei hepatopancreas during hypoxia and reoxygenation","volume":"143","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1016\/j.cub.2004.06.051","article-title":"Identification of the gene encoding bursicon, an insect neuropeptide responsible for cuticle sclerotization and wing spreading","volume":"14","author":"Dewey","year":"2004","journal-title":"Curr. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.ygcen.2006.12.004","article-title":"Evolutionary conservation of bursicon in the animal kingdom","volume":"153","author":"Vandersmissen","year":"2007","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Chung, J.S., Katayama, H., and Dircksen, H. (2012). New Functions of Arthropod Bursicon: Inducing Deposition and Thickening of new cuticle and hemocyte granulation in the blue crab, Callinectes sapidus. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0046299"}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/1\/84\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:30:00Z","timestamp":1759919400000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/1\/84"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,16]]},"references-count":42,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["biology14010084"],"URL":"https:\/\/doi.org\/10.3390\/biology14010084","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,16]]}}}