{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T23:32:41Z","timestamp":1788478361454,"version":"build-2803163510"},"reference-count":38,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Right ventricular (RV) failure is a common complication in multiple congenital heart disease (CHD), significantly increasing morbidity and mortality. Despite its impact, no therapies specifically target the failing RV. The growing population of CHD patients underscores the need to understand the pathophysiology of RV failure through preclinical research. This study aimed to develop an ovine model of RV failure induced by pressure and volume overload. A total of 14 juvenile sheep randomly underwent pulmonary artery banding (n = 6), pulmonary leaflet perforation (n = 4), and pulmonary annulotomy with transannular patching (TAP) (n = 4). Detailed anesthetic and surgical protocols were described, and intraoperative and early postoperative complications were evaluated. Acute RV pressure overload resulted in a 120% increase in RV pressure (p = 0.0312). The stroke volume index and cardiac index significantly declined (p = 0.0312), and there was a significant decrease in ScvO2 (p = 0.0312). Both TAP and leaflet perforation achieved moderate-to-severe pulmonary regurgitation. Both procedures led to an incremental trend in RV pressures and resulted in a 24% increase in the stroke volume index. All techniques demonstrated safety and feasibility, with low mortality. This comprehensive model could be reproducible in other large animal models, offering a robust platform for preclinical research into CHD-RV failure models.<\/jats:p>","DOI":"10.3390\/biology14020170","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T04:00:28Z","timestamp":1738900828000},"page":"170","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Early Outcomes of Right Ventricular Pressure and Volume Overload in an Ovine Model"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2509-3779","authenticated-orcid":false,"given":"Hamida","family":"Al Hussein","sequence":"first","affiliation":[{"name":"Doctoral School, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"},{"name":"Department of Anesthesiology and Critical Care, Clinical County Hospital Mures, 1 Gheorghe Marinescu Street, 540103 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4742-3583","authenticated-orcid":false,"given":"Hussam","family":"Al Hussein","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"},{"name":"Regenerative Medicine Laboratory, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"},{"name":"Department of Cardiovascular Surgery, Emergency Institute for Cardiovascular Diseases and Transplantation, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9044-8866","authenticated-orcid":false,"given":"Marius Mihai","family":"Harpa","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Laboratory, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"},{"name":"Department of Cardiovascular Surgery, Emergency Institute for Cardiovascular Diseases and Transplantation, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania"},{"name":"Department of Surgery, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5460-8087","authenticated-orcid":false,"given":"Simina Elena Rusu","family":"Ghiragosian","sequence":"additional","affiliation":[{"name":"Department of Pediatrics III, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"},{"name":"Department of Pediatric Cardiology, Emergency Institute for Cardiovascular Diseases and Transplantation, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3968-5118","authenticated-orcid":false,"given":"Simona","family":"Gurzu","sequence":"additional","affiliation":[{"name":"Department of Morphopathology, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"},{"name":"Department of Morphopathology, Emergency Clinical County Hospital, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0042-866X","authenticated-orcid":false,"given":"Bogdan","family":"Cordos","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Laboratory, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"},{"name":"Experimental Station, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0996-284X","authenticated-orcid":false,"given":"Carmen","family":"Sircuta","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care, Emergency Institute for Cardiovascular Diseases and Transplantation, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0447-4129","authenticated-orcid":false,"given":"Alexandra Iulia","family":"Puscas","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Emergency Institute for Cardiovascular Diseases and Transplantation, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9956-2312","authenticated-orcid":false,"given":"David Emanuel","family":"Anitei","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Emergency Institute for Cardiovascular Diseases and Transplantation, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7088-3742","authenticated-orcid":false,"given":"Cynthia","family":"Lefter","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2390-4291","authenticated-orcid":false,"given":"Horatiu","family":"Suciu","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Emergency Institute for Cardiovascular Diseases and Transplantation, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania"},{"name":"Department of Surgery, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7117-9904","authenticated-orcid":false,"given":"Dan","family":"Simionescu","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Laboratory, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"},{"name":"Biocompatibility and Tissue Regeneration Laboratory, Department of Bioengineering, Clemson University, Sikes Hall, Clemson, SC 29634, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4852-6787","authenticated-orcid":false,"given":"Klara","family":"Brinzaniuc","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"i27","DOI":"10.1136\/hrt.2005.077438","article-title":"The right ventricle in congenital heart disease","volume":"92","author":"Davlouros","year":"2006","journal-title":"Heart"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.ppedcard.2011.06.002","article-title":"New Directions in Basic Research in Hypertrophy and Heart Failure: Relevance for Pediatric Cardiology","volume":"32","author":"Bernstein","year":"2011","journal-title":"Prog. 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Surg."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/2\/170\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:28:35Z","timestamp":1760027315000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/2\/170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,7]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["biology14020170"],"URL":"https:\/\/doi.org\/10.3390\/biology14020170","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,7]]}}}