{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T22:16:06Z","timestamp":1788473766037,"version":"build-2803163510"},"reference-count":46,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T00:00:00Z","timestamp":1740355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Fishery Science, Ministry of Ocean and Fisheries, Korea","award":["R2025019"],"award-info":[{"award-number":["R2025019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Olive flounder (Paralichthys olivaceus) is a commercially important fish species in Japan, China, and the Republic of Korea. Despite numerous attempts to improve productivity, there have been no studies of in vitro germline stem cell (GSC) culture in this species. Here, olive flounder testicular and ovarian cell lines (OFT and OFO, respectively) were established and characterized. RT-PCR demonstrated that OFT and OFO expressed several gonadal somatic cell markers, including wt1 and fgf2, but lacked expression of germ cell markers, such as vasa, nanos2, and scp3. In addition, SNP analysis revealed that OFT originated from XY male P. olivaceus and OFO originated from XX female P. olivaceus. These results suggest that OFT was composed of Sertoli cells and OFO was composed of granulosa cells and theca cells. Finally, coculture of OFT or OFO with enriched male P. olivaceus GSCs isolated from the top 20% and 20\u201330% Percoll density gradient layers showed that GSCs were attached on both cell lines. In conclusion, we established P. olivaceus testicular and ovarian cell lines, which were expected to use for development of an in vitro GSC culture system.<\/jats:p>","DOI":"10.3390\/biology14030229","type":"journal-article","created":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T06:47:17Z","timestamp":1740379637000},"page":"229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Establishment and Characterization of OFT and OFO Cell Lines from Olive Flounder (Paralichthys olivaceus) for Use as Feeder Cells"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4549-7570","authenticated-orcid":false,"given":"Ja Young","family":"Jo","sequence":"first","affiliation":[{"name":"Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4220-711X","authenticated-orcid":false,"given":"Ju-Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3880-5050","authenticated-orcid":false,"given":"Eun Soo","family":"Noh","sequence":"additional","affiliation":[{"name":"Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Ok","family":"Kim","sequence":"additional","affiliation":[{"name":"Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9358-9568","authenticated-orcid":false,"given":"Seung Pyo","family":"Gong","sequence":"additional","affiliation":[{"name":"Major in Aquaculture and Applied Life Science, Division of Fisheries Life Science, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6524-6544","authenticated-orcid":false,"given":"Hee Jeong","family":"Kong","sequence":"additional","affiliation":[{"name":"Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1933-4153","authenticated-orcid":false,"given":"Jae Hoon","family":"Choi","sequence":"additional","affiliation":[{"name":"Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.ygcen.2009.05.022","article-title":"Oogenesis in Teleosts: How Fish Eggs Are Formed","volume":"165","author":"Lubzens","year":"2010","journal-title":"Gen. 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Cell"}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/3\/229\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:41:23Z","timestamp":1760028083000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/3\/229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,24]]},"references-count":46,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["biology14030229"],"URL":"https:\/\/doi.org\/10.3390\/biology14030229","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,24]]}}}