{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:09:08Z","timestamp":1760123348442,"version":"build-2065373602"},"reference-count":26,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Health system through the Veneto Region"},{"name":"ARCA research Foundation, Padua, Italy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>A biopsy involves the removal of a piece or an entire organ from a living patient. The former began with open heart surgery (surgical pathology) and the latter with the recipient heart in cardiac transplantation. Transvenous or transarterial catheterization is the current procedure to performed endomyocardial biopsy with bioptome from the ventricles. This manoeuvre was first carried out by Werner Forssmann through a urological catheter in 1929, which he introduced into his radial left vein until it reached the RV. Then, in London in 1974, Richardson invented a new technique with a catheter via the right femoral vein, which he applied with success in patients with multiple myocardial diseases, both inflammatory and non-inflammatory. Subsequently, a transjugular endomyocardial biopsy was accomplished by Margaret Billingham to monitor heart rejection during cardiac transplantation. In the beginning, only histology for a light microscope, and rarely during electron microscopy, was employed. With the advent of molecular techniques and the discovery of polymerase chain reaction (PCR), molecular investigation became part of the gold standard for diagnosis involving EMB: histology, immunohistochemistry and molecular investigation, the latter in search of a viral cause. Nowadays, EMB is frequently employed in infiltrative (amyloidosis) and storage diseases (e.g., hemochromatosis and Fabry diseases). Diagnosis of myocarditis is now possible through Magnetic Cardiac Resonance (MCR), in place of BEM histology, thanks to oedema. With the help of ECMO, it is possible to allow the heart to rest, supporting its recovery from ejection fraction even in fulminant myocarditis. Cardiac transplantation with the pathological study of the recipient heart offers the opportunity to discover and study new diseases, like restrictive cardiomyopathy and a non-compacted left ventricle.<\/jats:p>","DOI":"10.3390\/biology14030306","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T11:02:31Z","timestamp":1742295751000},"page":"306","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Storytelling of Myocardial Biopsy"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8814-2577","authenticated-orcid":false,"given":"Gaetano","family":"Thiene","sequence":"first","affiliation":[{"name":"Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padua, Via A. Gabelli, 86-35121 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1161\/01.RES.1.3.226","article-title":"Metabolic Studies on Cardiac Tissue Obtained by Needle Biopsy in the Intact Unanesthetized Dog","volume":"1","author":"Casten","year":"1953","journal-title":"Circ. Res."},{"key":"ref_2","first-page":"213","article-title":"Needle Biopsy of the Human Ventricular Myocardium","volume":"30","author":"Kent","year":"1956","journal-title":"Q. Bull. Northwest Univ. Med. Sch."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1536\/ihj.3.537","article-title":"Endomyocardial Biopsy","volume":"3","author":"Sakakibara","year":"1962","journal-title":"Jpn. Heart J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1016\/S0140-6736(74)93204-8","article-title":"King\u2019s Endomyocardial Bioptome","volume":"1","author":"Richardson","year":"1974","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1016\/S0022-5223(19)34001-2","article-title":"Cardiac Fibroma and Heart Transplantation","volume":"106","author":"Valente","year":"1993","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0002-8703(61)90513-0","article-title":"\u2018Auricularization\u2019 of Right Ventricular Pressure Curve","volume":"61","author":"Battaglia","year":"1961","journal-title":"Am. Heart J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1007\/BF01875120","article-title":"Die Sondierung des rechten Herzens","volume":"8","author":"Forssmann","year":"1929","journal-title":"Klin. Wochenschr."},{"key":"ref_8","unstructured":"Martyn, J., and Allestry, J. (1665). Micrographia, or, Some Physiological Descriptions of Minute Bodies Made by Magnifying Glasses with Observations and Inquiries Thereupon, Royal Society."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/0002-9149(78)90741-5","article-title":"Myocardial Biopsy: A Useful Diagnostic Procedure or Only a Research Tool?","volume":"41","author":"Ferrans","year":"1978","journal-title":"Am. J. Cardiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/1054-8807(93)90018-W","article-title":"In Vivo Diagnosis of Cardiac Angiosarcoma by Endomyocardial Biopsy","volume":"2","author":"Poletti","year":"1993","journal-title":"Cardiovasc. Pathol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/S1010-7940(01)00578-4","article-title":"Cardiogenic Shock Due to Metastatic Cardiac Lymphoma: Still a Diagnostic and Therapeutic Challenge","volume":"19","author":"Testolin","year":"2001","journal-title":"Eur. J. Cardiothorac. Surg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/S0002-8703(96)90374-9","article-title":"Primary Right Atrial Fibrosarcoma Diagnosed by Endocardial Biopsy","volume":"131","author":"Basso","year":"1996","journal-title":"Am. Heart J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.jcmg.2013.10.016","article-title":"Cardiac Magnetic Resonance Features of Biopsy-Proven Endomyocardial Diseases","volume":"7","author":"Thiene","year":"2014","journal-title":"JACC Cardiovasc. Imaging"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.carpath.2011.10.001","article-title":"2011 Consensus Statement on Endomyocardial Biopsy from the Association for European Cardiovascular Pathology and the Society for Cardiovascular Pathology","volume":"21","author":"Leone","year":"2012","journal-title":"Cardiovasc. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1161\/CIRCULATIONAHA.107.186093","article-title":"The Role of Endomyocardial Biopsy in the Management of Cardiovascular Disease: A Scientific Statement from the American Heart Association, the American College of Cardiology, and the European Society of Cardiology","volume":"116","author":"Cooper","year":"2007","journal-title":"Circulation"},{"key":"ref_16","unstructured":"Mullis, K.B., Erlich, H.A., Gelfand, D.H., Horn, G., and Saiki, R.K. (1986). Process for Amplifying, Detecting, and\/or Cloning Nucleic Acid Sequences. (4,683,195), U.S. Patent."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1007\/s10741-012-9355-6","article-title":"Classification and Histological, Immunohistochemical, and Molecular Diagnosis of Inflammatory Myocardial Disease","volume":"18","author":"Basso","year":"2013","journal-title":"Heart Fail. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00428-013-1430-4","article-title":"Diagnostic Use of the Endomyocardial Biopsy: A Consensus Statement","volume":"463","author":"Thiene","year":"2013","journal-title":"Virchows Arch."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2760","DOI":"10.1093\/eurheartj\/ehn415","article-title":"Quantitative Assessment of Endomyocardial Biopsy in Arrhythmogenic Right Ventricular Cardiomyopathy\/Dysplasia: An In Vitro Validation of Diagnostic Criteria","volume":"29","author":"Basso","year":"2008","journal-title":"Eur. Heart J."},{"key":"ref_20","first-page":"3","article-title":"Myocarditis: A Histopathologic Definition and Classification","volume":"1","author":"Aretz","year":"1987","journal-title":"Am. J. Cardiovasc. Pathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1161\/CIRCULATIONAHA.105.589663","article-title":"Diagnosis of Myocarditis: Death of Dallas Criteria","volume":"113","author":"Baughman","year":"2006","journal-title":"Circulation"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/S0022-5223(19)42526-9","article-title":"Development of a Membrane Oxygenator: Overcoming Blood Diffusiolimitation","volume":"58","author":"Bartlett","year":"1969","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1067\/mtc.2001.115243","article-title":"Mechanical Circulatory Support for the Treatment of Children with Acute Fulminant Myocarditis","volume":"122","author":"Duncan","year":"2001","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.jpeds.2010.10.015","article-title":"Management and Outcomes in Pediatric Patients Presenting with Acute Fulminant Myocarditis","volume":"158","author":"Teele","year":"2011","journal-title":"J. Pediatr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1097\/CCM.0000000000000920","article-title":"Extracorporeal Membrane Oxygenation for the Support of Adults with Acute Myocarditis","volume":"43","author":"Diddle","year":"2015","journal-title":"Crit. Care Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/j.athoracsur.2015.08.014","article-title":"Venoarterial Extracorporeal Membrane Oxygenation for Acute Fulminant Myocarditis in Adult Patients: A 5-Year Multi-Institutional Experience","volume":"101","author":"Lorusso","year":"2016","journal-title":"Ann. Thorac. Surg."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/3\/306\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:56:09Z","timestamp":1760028969000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/3\/306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,18]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["biology14030306"],"URL":"https:\/\/doi.org\/10.3390\/biology14030306","relation":{},"ISSN":["2079-7737"],"issn-type":[{"type":"electronic","value":"2079-7737"}],"subject":[],"published":{"date-parts":[[2025,3,18]]}}}