{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:34:10Z","timestamp":1788381250261,"version":"build-2803163510"},"reference-count":74,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007514","name":"University of Pisa","doi-asserted-by":"publisher","award":["PRA_2020_88."],"award-info":[{"award-number":["PRA_2020_88."]}],"id":[{"id":"10.13039\/501100007514","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Wild ruminants often harbor pathogens transmissible to other animals and humans, but their epidemiological role is not always defined for all microorganisms. In this survey, spleens, kidneys, and hearts sampled from 162 fallow deer (Dama dama) were subjected to molecular analyses to detect bacterial (Anaplasma phagocytophilum, Borrelia burgdorferi s.l., Brucella spp., Chlamydia abortus, Coxiella burnetii, Francisella tularensis, Leptospira spp.) and protozoan (piroplasms, Neospora caninum, Toxoplasma gondii) pathogens. Five (3.08%) spleens were positive for A. phagocytophilum, and twelve (7.40%) spleens were positive for Theileria cervi. The remaining pathogens investigated were not detected, and no coinfections were found. The analyzed animals do not seem to have a relevant role in the spreading of these pathogens; however, monitoring is pivotal to understand the epidemiological scenarios and take appropriate preventive measures in areas frequently visited by people.<\/jats:p>","DOI":"10.3390\/biology14040342","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T04:11:40Z","timestamp":1743135100000},"page":"342","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Presence of Bacterial and Protozoan Pathogens in Wild Fallow Deer (Dama dama) from a Protected Area in Central Italy"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7997-776X","authenticated-orcid":false,"given":"Valentina Virginia","family":"Ebani","sequence":"first","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy"},{"name":"Centre for Climate Change Impact, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6069-4265","authenticated-orcid":false,"given":"Paolo","family":"Bongi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Trebino","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5292-0613","authenticated-orcid":false,"given":"Fabrizio","family":"Bertelloni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8198-5865","authenticated-orcid":false,"given":"Giulia","family":"Cagnoli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedetta","family":"Bigliazzi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9519-364X","authenticated-orcid":false,"given":"Marco","family":"Del Frate","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8953-9138","authenticated-orcid":false,"given":"Marco","family":"Apollonio","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Sassari, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3301-3778","authenticated-orcid":false,"given":"Francesca","family":"Mancianti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Silaghi, C., Fr\u00f6hlich, J., Reindl, H., Hamel, D., and Rehbein, S. 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