{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T01:58:29Z","timestamp":1782352709222,"version":"3.54.5"},"reference-count":44,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T00:00:00Z","timestamp":1747872000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82060747"],"award-info":[{"award-number":["82060747"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["202405AF140044"],"award-info":[{"award-number":["202405AF140044"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Yunnan Expert Workstation","award":["82060747"],"award-info":[{"award-number":["82060747"]}]},{"name":"Yunnan Expert Workstation","award":["202405AF140044"],"award-info":[{"award-number":["202405AF140044"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Ischemic stroke (IS) is a high-mortality, multi-complication cardiovascular disease. Reducing brain injury and promoting neuronal repair after IS onset remain important challenges for current treatments. Our team previously found that PAS840, an extract from Periplaneta americana (L.), protects nerve function; this study further uses LC-MS\/MS and peptidomics to analyze PAS840\u2019s components and network pharmacology to predict its ischemic stroke (IS) therapeutic targets. We then employed Transwell, a biochemical kit, real-time quantitative polymerase chain reaction (RT-qPCR), and transcriptomics to investigate PAS840\u2019s effects on migration ability, oxidative stress levels, and cellular pathways in mouse microglial cells (BV-2) following oxygen\u2013glucose deprivation\/reoxygenation (OGD\/R) injury. Finally, using Evans blue staining, immunohistochemical analysis, and RT-qPCR, we investigated PAS840\u2019s effects on the blood\u2013brain barrier, inflammation pathways, and neural function in a transient middle cerebral artery occlusion (tMCAO) rat model. PAS840 components target multiple IS pathways, effectively inhibit NF-\u03baB\/NLRP3\/Caspase-1\/IL-1\u03b2 inflammasome pathway activation in BV-2 cells following OGD\/R, reduce cellular oxidative stress, inflammation, and pyroptosis, and improve cell viability and migration ability. PAS840 decreases NF-\u03baB\/NLRP3\/Caspase-1\/IL-1\u03b2 inflammasome pathway expression in tMCAO rat brains, reduces inflammation, activates BDNF\/VGF\/NGR1\/Erbb4 neurotrophic factor and vascular endothelial growth factor pathways, enhances neuronal cell viability, and effectively protects and repairs the blood\u2013brain barrier.<\/jats:p>","DOI":"10.3390\/biology14060589","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T12:15:43Z","timestamp":1747916143000},"page":"589","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Periplaneta americana (L.) Extract PAS840 Promotes Ischemic Stroke Recovery by Inhibiting Inflammasome Activation"],"prefix":"10.3390","volume":"14","author":[{"given":"Xin","family":"Yang","sequence":"first","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Canhui","family":"Hong","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tangfei","family":"Guan","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenggui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongshou","family":"Yang","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"},{"name":"Engineering Research Center for Development of the Periplaneta Americana Industry of Yunnan Provincial Department of Education, Dali University, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyun","family":"Xiao","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"},{"name":"Engineering Research Center for Development of the Periplaneta Americana Industry of Yunnan Provincial Department of Education, Dali University, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huai","family":"Xiao","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8260-868X","authenticated-orcid":false,"given":"Zhengchun","family":"He","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1161\/STROKEAHA.118.023118","article-title":"Stroke and Cerebrovascular Disease in Pregnancy","volume":"50","author":"Liu","year":"2019","journal-title":"Stroke"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1177\/17474930211065917","article-title":"World Stroke Organization (WSO): Global Stroke Fact Sheet 2022","volume":"17","author":"Feigin","year":"2022","journal-title":"Int. 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