{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:00:47Z","timestamp":1790398847614,"version":"4.1.0"},"reference-count":60,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["82060747"],"award-info":[{"award-number":["82060747"]}]},{"name":"National Natural Science Foundation of China","award":["202405AF140044"],"award-info":[{"award-number":["202405AF140044"]}]},{"name":"Yunnan Expert Workstation","award":["82060747"],"award-info":[{"award-number":["82060747"]}]},{"name":"Yunnan Expert Workstation","award":["202405AF140044"],"award-info":[{"award-number":["202405AF140044"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Androgenetic alopecia (AGA), the most prevalent form of hair loss worldwide, faces significant therapeutic challenges due to high costs and limited efficacy of current interventions, necessitating safer and more effective solutions. Periplaneta americana (L.)-derived PA-011, endowed with anti-inflammatory and antioxidant properties, has demonstrated notable hair growth-promoting effects in AGA mouse models. This study employed LC-MS\/MS, peptidomics, and network pharmacology to characterize PA-011\u2019s chemical composition and predict its potential targets in AGA pathogenesis. Using Western blot and RT-qPCR, PA-011 intervention significantly inhibited inflammatory responses and oxidative stress levels in mouse skin tissues. Concurrently, PA-011 activated the proliferative potential of hair follicle stem cells, as demonstrated by upregulated expression of the cell proliferation marker Ki67, and activated the Wnt\/\u03b2-catenin signaling pathway in DHT-induced AGA mice. Transcriptomic and metabolomic analyses revealed multi-target effects of PA-011, including modulation of PI3K-Akt\/MAPK pathways, pentose phosphate metabolism, and amino acid biosynthesis. 16S rRNA sequencing and metagenomic analysis showed that AGA disrupts skin microbial homeostasis, while PA-011 intervention normalized the microbiota composition. Topical application of PA-011 promoted robust hair regrowth without detectable toxicity in safety assessments. This preclinical study establishes PA-011 as a promising candidate for AGA therapy, warranting further translational investigation.<\/jats:p>","DOI":"10.3390\/biology14070831","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T09:12:48Z","timestamp":1751965968000},"page":"831","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring the Efficacy and Potential Mechanisms of Topical Periplaneta americana (L.) Extract in Treating Androgenetic Alopecia in a Mouse Model: A Systems Pharmacology and Skin Microbiome Analysis"],"prefix":"10.3390","volume":"14","author":[{"given":"Tangfei","family":"Guan","sequence":"first","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"},{"name":"West China School of Public Health, Sichuan University, Chengdu 610041, China"},{"name":"West China Fourth Hospital, Sichuan University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Yang","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Canhui","family":"Hong","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7219-6813","authenticated-orcid":false,"given":"Peiyun","family":"Xiao","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongshou","family":"Yang","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenggui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8260-868X","authenticated-orcid":false,"given":"Zhengchun","family":"He","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.jaad.2019.03.079","article-title":"Medical therapy for frontal fibrosing alopecia: A review and clinical approach","volume":"81","author":"Ho","year":"2019","journal-title":"J. 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