{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:46:24Z","timestamp":1787741184337,"version":"build-2784847793"},"reference-count":63,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T00:00:00Z","timestamp":1753228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32402983"],"award-info":[{"award-number":["32402983"]}]},{"name":"National Natural Science Foundation of China","award":["2024KJG006"],"award-info":[{"award-number":["2024KJG006"]}]},{"name":"National Natural Science Foundation of China","award":["JBGS [2021] 034"],"award-info":[{"award-number":["JBGS [2021] 034"]}]},{"name":"Qingchuang science and technology support program of Shandong provincial college","award":["32402983"],"award-info":[{"award-number":["32402983"]}]},{"name":"Qingchuang science and technology support program of Shandong provincial college","award":["2024KJG006"],"award-info":[{"award-number":["2024KJG006"]}]},{"name":"Qingchuang science and technology support program of Shandong provincial college","award":["JBGS [2021] 034"],"award-info":[{"award-number":["JBGS [2021] 034"]}]},{"name":"\u201cJBGS\u201d Project of Seed Industry Revitalization in Jiangsu Province","award":["32402983"],"award-info":[{"award-number":["32402983"]}]},{"name":"\u201cJBGS\u201d Project of Seed Industry Revitalization in Jiangsu Province","award":["2024KJG006"],"award-info":[{"award-number":["2024KJG006"]}]},{"name":"\u201cJBGS\u201d Project of Seed Industry Revitalization in Jiangsu Province","award":["JBGS [2021] 034"],"award-info":[{"award-number":["JBGS [2021] 034"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Hypoxia represents a critical environmental stressor in aquaculture, significantly disrupting aquatic organisms\u2019 physiological homeostasis and thereby constraining the sustainable development of aquaculture industries. To elucidate the mechanisms underlying hypoxia-induced metabolic regulation in aquatic species, this study employed hybrid yellow catfish (Pelteobagrus vachelli \u2640 \u00d7 Leiocassis longirostris \u2642) as a model organism to systematically investigate the multidimensional physiological responses in brain, liver, and muscle tissues under hypoxia (0.7 mg\/L) and reoxygenation (7.0 mg\/L) conditions. Through qRT-PCR and enzymatic activity analyses, we comprehensively assessed molecular alterations associated with oxygen sensing (HIF-1\u03b1 gene), respiratory metabolism (PFKL, HK1, PK, CS, and LDHA genes and corresponding enzyme activities), oxidative stress (SOD1, SOD2, GSH-PX, and CAT genes, along with LPO, MDA, PCO, T-SOD, GSH-PX, and CAT levels), apoptosis (Caspase-3, Bax\/Bcl-2), inflammatory response (IL-1\u03b2, IKK\u03b2), and mitochondrial function (COXIV, PGC-1\u03b1, ATP5A1). Key findings demonstrated pronounced HIF-1\u03b1 activation across all examined tissues. Hepatic tissues exhibited adaptive metabolic reprogramming from aerobic to anaerobic metabolism, whereas cerebral tissues displayed suppressed anaerobic glycolysis during prolonged hypoxia, and muscular tissues manifested concurrent inhibition of both glycolytic and aerobic metabolic pathways. Notably, skeletal muscle exhibited marked oxidative stress accompanied by mitochondrial dysfunction, exacerbated inflammation, and apoptosis activation during hypoxia\/reoxygenation cycles. This study delineates tissue-specific adaptive mechanisms to hypoxia in yellow catfish, providing theoretical foundations for both piscine hypoxia physiology research and aquaculture practices.<\/jats:p>","DOI":"10.3390\/biology14080915","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T11:52:47Z","timestamp":1753271567000},"page":"915","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Hypoxia and Reoxygenation on Hypoxia-Responsive Genes, Physiological and Biochemical Indices in Hybrid Catfish (Pelteobagrus vachelli \u2640 \u00d7 Leiocassis longirostris \u2642)"],"prefix":"10.3390","volume":"14","author":[{"given":"Jie","family":"Yan","sequence":"first","affiliation":[{"name":"College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210098, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faling","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210098, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenfei","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Agriculture and Bioengineering, Heze University, Heze 274015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210098, China"},{"name":"Co-Innovation Center for Marine Bio-Industry Technology, Lian Yungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaowu","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210098, China"},{"name":"Co-Innovation Center for Marine Bio-Industry Technology, Lian Yungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guosong","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Agriculture and Bioengineering, Heze University, Heze 274015, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"eaba3512","DOI":"10.1126\/science.aba3512","article-title":"Oxygen-sensing mechanisms across eukaryotic kingdoms and their roles in complex multicellularity","volume":"370","author":"Hammarlund","year":"2020","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"734484","DOI":"10.1016\/j.aquaculture.2019.734484","article-title":"Hypoxia tolerance in a selectively bred F4 population of blunt snout bream (Megalobrama amblycephala) under hypoxic stress","volume":"518","author":"Wu","year":"2020","journal-title":"Aquaculture"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1111\/gcb.13193","article-title":"Global spread of hypoxia in freshwater ecosystems during the last three centuries is caused by rising local human pressure","volume":"22","author":"Jenny","year":"2016","journal-title":"Glob. 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