{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:09:26Z","timestamp":1790860166448,"version":"4.1.0"},"reference-count":56,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T00:00:00Z","timestamp":1756425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key R&amp;D and Promotion Projects of Henan Province","award":["242102311256"],"award-info":[{"award-number":["242102311256"]}]},{"name":"Key R&amp;D and Promotion Projects of Henan Province","award":["HNFZ20240137"],"award-info":[{"award-number":["HNFZ20240137"]}]},{"name":"Key R&amp;D and Promotion Projects of Henan Province","award":["23A610008"],"award-info":[{"award-number":["23A610008"]}]},{"name":"Henan Province \u201cDeputy Chief Scientist for Science and Technology\u201d Program","award":["242102311256"],"award-info":[{"award-number":["242102311256"]}]},{"name":"Henan Province \u201cDeputy Chief Scientist for Science and Technology\u201d Program","award":["HNFZ20240137"],"award-info":[{"award-number":["HNFZ20240137"]}]},{"name":"Henan Province \u201cDeputy Chief Scientist for Science and Technology\u201d Program","award":["23A610008"],"award-info":[{"award-number":["23A610008"]}]},{"name":"Key Scientific Research Projects of Colleges and Universities in Henan Province","award":["242102311256"],"award-info":[{"award-number":["242102311256"]}]},{"name":"Key Scientific Research Projects of Colleges and Universities in Henan Province","award":["HNFZ20240137"],"award-info":[{"award-number":["HNFZ20240137"]}]},{"name":"Key Scientific Research Projects of Colleges and Universities in Henan Province","award":["23A610008"],"award-info":[{"award-number":["23A610008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Harmful cyanobacterial blooms (CyanoHABs) threaten freshwater ecosystems and human health. Inhibiting cyanobacteria through plant allelopathy is an effective and environmentally friendly approach for CyanoHAB control. In this study, we evaluated the inhibitory activities of several organic solvent extracts from Artemisia argyi against the common bloom-forming cyanobacterium Microcystis aeruginosa, explored the anti-algal mechanism of the active fraction, analyzed its secondary metabolites using liquid chromatography\u2013high-resolution mass spectrometry (LC-HRMS), and screened the potential allelochemicals. The results showed that the crude extract of A. argyi leaves (CE) exhibited significant inhibitory effects on M. aeruginosa. Among several solvent fractions of CE, the dichloromethane extract (DE) demonstrated the strongest inhibitory effect, with a 7-day IC50 of 70.43 mg\/L. After treatment with DE, the contents of chlorophyll a (Chl a), carotenoids, and phycobiliproteins (PBPs) in M. aeruginosa were significantly reduced. Meanwhile, an excessive accumulation of reactive oxygen species (ROS), reduction of catalase (CAT) activity, increase in malondialdehyde (MDA) content, and shrinkage of the membrane were found in M. aeruginosa cells under DE treatments. There were 81 secondary metabolites annotated in DE by LC-HRMS. Among them, hispidulin, jaceosidin, 5,7,3\u2032-trihydroxy-6,4\u2032,5\u2032-trimethoxyflavone, and eupatilin possessed strong inhibitory activities, with 7-day IC50 values of 26.23, 27.62, 32.02, and 34.98 mg\/L, respectively. These results indicated that the A. argyi extracts possess significant allelopathic activities on M. aeruginosa, and DE was identified as the primary active fraction. It inhibits algae growth by suppressing photosynthesis and inducing peroxidation, ultimately leading to cell death. Flavonoids in DE were the main allelochemicals responsible for the inhibition on algae of A. argyi extracts.<\/jats:p>","DOI":"10.3390\/biology14091141","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T16:18:15Z","timestamp":1756484295000},"page":"1141","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Inhibitory Effects of Artemisia argyi Extracts on Microcystis aeruginosa: Anti-Algal Mechanisms and Main Allelochemicals"],"prefix":"10.3390","volume":"14","author":[{"given":"Jiajia","family":"Dong","sequence":"first","affiliation":[{"name":"School of Life Sciences, Nanyang Normal University, Nanyang 473061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9493-1222","authenticated-orcid":false,"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Nanyang Normal University, Nanyang 473061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalei","family":"Du","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Nanyang Normal University, Nanyang 473061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5692-1146","authenticated-orcid":false,"given":"Lingling","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Water Resources and Modern Agriculture, Nanyang Normal University, Nanyang 473061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1697-7399","authenticated-orcid":false,"given":"Zhiqiang","family":"Yan","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Nanyang Normal University, Nanyang 473061, China"},{"name":"Collaborative Innovation Center of Water Security for Water Source Region of Mid-Line of South-to-North Diversion Project of Henan Province, Nanyang Normal University, Nanyang 473061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1146\/annurev-environ-112320-081653","article-title":"Harmful cyanobacterial blooms: Biological traits, mechanisms, risks, and control strategies","volume":"48","author":"Song","year":"2023","journal-title":"Annu. Rev. Environ. Resour."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.biortech.2017.07.175","article-title":"Microorganisms-based methods for harmful algal blooms control: A review","volume":"248","author":"Sun","year":"2018","journal-title":"Bioresour. Technol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1007\/s10811-011-9751-7","article-title":"Harmful algal blooms mitigation using clay\/soil\/sand modified with xanthan and calcium hydroxide","volume":"24","author":"Chen","year":"2012","journal-title":"J. Appl. Phycol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zhu, X., Dao, G., Tao, Y., Zhan, X., and Hu, H. (2021). A review on control of harmful algal blooms by plant-derived allelochemicals. J. Hazard. Mater., 401.","DOI":"10.1016\/j.jhazmat.2020.123403"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Li, B., Yin, Y., Kang, L., Feng, L., Liu, Y., Du, Z., Tian, Y., and Zhang, L. (2021). A review: Application of allelochemicals in water ecological restoration\u2014Algal inhibition. Chemosphere, 267.","DOI":"10.1016\/j.chemosphere.2020.128869"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.limno.2017.02.006","article-title":"Macrophytes-cyanobacteria allelopathic interactions and their implications for water resources management\u2014A review","volume":"63","author":"Mohamed","year":"2017","journal-title":"Limnologica"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.ecoleng.2018.12.026","article-title":"Comparative study of six emergent macrophyte species for controlling cyanobacterial blooms in a tropical reservoir","volume":"129","author":"Mowe","year":"2019","journal-title":"Ecol. Eng."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1590\/s2179-975x1419","article-title":"Allelopathic effects of the aquatic macrophyte Ceratophyllum demersum L. on phytoplankton species: Contrasting effects between cyanobacteria and chlorophytes","volume":"31","author":"Amorim","year":"2019","journal-title":"Acta Limnol. Bras."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.ecoleng.2016.07.021","article-title":"Selective growth inhibition of cyanobacteria species (Planktothrix agardhii) by a riparian tree leaf extract","volume":"97","author":"Brient","year":"2016","journal-title":"Ecol. Eng."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"42276","DOI":"10.1007\/s11356-020-08651-1","article-title":"The extract of aquatic macrophyte Carex cinerascens induced colony formation in bloom-forming cyanobacterium Microcystis aeruginosa","volume":"27","author":"Li","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_11","first-page":"490","article-title":"Isolating and identifying organic acids from Portulaca oleracea and determining their anti-cyanobacterial activity","volume":"23","author":"Wang","year":"2017","journal-title":"Ecol. Environ. Conserv."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/j.1365-3180.2006.00509.x","article-title":"Herbicidal potential of allelochemicals from Lantana camara against Eichhornia crassipes and the alga Microcystis aeruginosa","volume":"46","author":"Kong","year":"2006","journal-title":"Weed Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1111\/1462-2920.12226","article-title":"A pair of chiral flavonolignans as novel anti-cyanobacterial allelochemicals derived from barley straw (Hordeum vulgare): Characterization and comparison of their anti-cyanobacterial activities","volume":"16","author":"Xiao","year":"2014","journal-title":"Environ. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Wu, L., Li, L., Dong, P., Zhang, L., Tang, H., Han, Y., and Xie, G. (2023). Allelopathy of p-coumaric acid on Limnothrix sp., a bloom-forming cyanobacteria. Algal Res., 75.","DOI":"10.1016\/j.algal.2023.103268"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.jenvman.2018.11.105","article-title":"Allelopathic inhibition of juglone (5-hydroxy-1,4-naphthoquinone) on the growth and physiological performance in Microcystis aeruginosa","volume":"232","author":"Hou","year":"2019","journal-title":"J. Environ. Manag."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Yin, L., Shi, K., Yin, Y., Zhang, Y., Xu, L., An, J., Peng, C., Wang, C., He, H., and Yang, S. (2025). Long-term suppression of Microcystis aeruginosa by tannic acid: Risks of microcystin pollution and proteomic mechanisms. J. Hazard. Mater., 487.","DOI":"10.1016\/j.jhazmat.2025.137205"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Chen, C., Li, J., Chen, H., Cai, H., Zhang, J., Zhang, Y., Zhang, Y., and Chen, J. (2024). Comprehensive review of botanical characteristics, artificial cultivation methods, quality evaluation, genome research, and potential applications of Artemisia argyi L\u00e9vl. et Van. Med. Plant Biol., 3.","DOI":"10.48130\/mpb-0024-0002"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Li, J., Chen, L., Chen, Q., Guo, Z., Sun, Y., Chen, J., and Li, J. (2021). Allelopathic effect of Artemisia argyi on the germination and growth of various weeds. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-83752-6"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Li, J., Zhao, T., Chen, L., Chen, Q., Guo, Z., Sun, Y., Chen, J., and Li, J. (2022). Artemisia argyi allelopathy: A generalist compromises hormone balance, element absorption, and photosynthesis of receptor plants. BMC Plant Biol., 22.","DOI":"10.1186\/s12870-022-03757-9"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1007\/s11101-023-09910-y","article-title":"Artemisia argyi H. L\u00e9v. & Vaniot: A comprehensive review on traditional uses, phytochemistry, and pharmacological activities","volume":"23","author":"Wei","year":"2024","journal-title":"Phytochem. Rev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7473","DOI":"10.1007\/s00210-024-03122-7","article-title":"A review of the research progress on Artemisia argyi Folium: Botany, phytochemistry, pharmacological activities, and clinical application","volume":"397","author":"Wang","year":"2024","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Liu, Y., He, Y., Wang, F., Xu, R., Yang, M., Ci, Z., Wu, Z., Zhang, D., and Lin, J. (2021). From longevity grass to contemporary soft gold: Explore the chemical constituents, pharmacology, and toxicology of Artemisia argyi H.L\u00e9v. & Vaniot essential oil. J. Ethnopharmacol., 279.","DOI":"10.1016\/j.jep.2021.114404"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1021\/acs.jafc.1c06417","article-title":"Weed suppression and molecular mechanisms of isochlorogenic acid A isolated from Artemisia argyi extract via an activity-guided method","volume":"70","author":"Chen","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Chen, L., Li, J., Zhu, Y., Guo, L., Ji, R., Miao, Y., Guo, L., Du, H., and Liu, D. (2022). Caffeic acid, an allelochemical in Artemisia argyi, inhibits weed growth via suppression of mitogen-activated protein kinase signaling pathway and the biosynthesis of gibberellin and phytoalexin. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.802198"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Xia, T., Bai, J., Qiu, Y., Peng, L., Yue, M., Wang, S., and Gao, X. (2024). Stress effects of allelopathic aqueous extracts in Artemisia argyi on Microcystis aeruginosa. Pol. J. Environ. Stud.","DOI":"10.15244\/pjoes\/192567"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/0076-6879(88)67034-0","article-title":"Phycobiliproteins in cyanobacteria","volume":"Volume 167","author":"Lester","year":"1988","journal-title":"Methods in Enzymology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s11306-007-0082-2","article-title":"Proposed minimum reporting standards for chemical analysis","volume":"3","author":"Sumner","year":"2007","journal-title":"Metabolomics"},{"key":"ref_28","unstructured":"Lopes, A.D., Nunes, M.G.I.F., Francisco, J.P., and dos Santos, E.H. (2022). Potential allelopathic effect of species of the Asteraceae family and its use in agriculture. Vegetation Dynamics, Changing Ecosystems and Human Responsibility, IntechOpen."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Ara\u00fajo, C.A., Morgado, C.S., Gomes, A.K.C., Gomes, A.C.C., and Simas, N.K. (2021). Asteraceae family: A review of its allelopathic potential and the case of Acmella oleracea and Sphagneticola trilobata. Rodrigu\u00e9sia, 72.","DOI":"10.1590\/2175-7860202172137"},{"key":"ref_30","first-page":"745","article-title":"Inhibitory effect of Artemisia lavandulaefolia and its organic extracts on Microcystis aeruginosa growth","volume":"30","author":"Xu","year":"2010","journal-title":"Acta Ecol. Sin."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"903","DOI":"10.15666\/aeer\/1601_903912","article-title":"Assessment of the potentially algicidal effects of Thymus satureioides Coss. and Artemisia herba alba L. against Microcystis aeruginosa","volume":"16","author":"Tebaa","year":"2018","journal-title":"Appl. Ecol. Environ. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1007\/s11426-011-4269-z","article-title":"Inhibitory effects of the extracts with different solvents from three Compositae plants on cyanobacterium Microcystis aeruginosa","volume":"54","author":"Ni","year":"2011","journal-title":"Sci. China Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1016\/j.chemosphere.2012.05.009","article-title":"Isolation and identification of an anti-algal compound from Artemisia annua and mechanisms of inhibitory effect on algae","volume":"88","author":"Ni","year":"2012","journal-title":"Chemosphere"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1007\/s11783-008-0065-1","article-title":"Inhibitive effects of three Compositae plants on Microcystis aeruginosa","volume":"3","author":"Zhang","year":"2009","journal-title":"Front. Environ. Sci. Eng. China"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jenvman.2013.04.001","article-title":"Potential for control of harmful cyanobacterial blooms using biologically derived substances: Problems and prospects","volume":"125","author":"Shao","year":"2013","journal-title":"J. Environ. Manag."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.2166\/wst.2020.225","article-title":"Allelopathic Effect of Rhubarb Extracts on the Growth of Microcystis aeruginosa","volume":"82","author":"Cao","year":"2020","journal-title":"Water Sci. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Cheng, J., Tan, L., Lu, X., Lin, S., and Lin, W. (2025). Photosynthetic toxicological effects of organic extracts from Zanthoxylum bungeanum leaves on controlling the Microcystis aeruginosa blooms. Curr. Microbiol., 82.","DOI":"10.1007\/s00284-024-04026-8"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Li, D., Li, P., Yan, Z., Li, N., Yao, L., and Cao, L. (2022). Allelopathic inhibition of the extracts of Landoltia punctata on Microcystis aeruginosa. Plant Signal. Behav., 17.","DOI":"10.1080\/15592324.2022.2058256"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"18994","DOI":"10.1007\/s11356-015-5104-4","article-title":"Study on the release routes of allelochemicals from Pistia stratiotes Linn., and its anti-cyanobacteria mechanisms on Microcystis aeruginosa","volume":"22","author":"Wu","year":"2015","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Budza\u0142ek, G., \u015aliwi\u0144ska-Wilczewska, S., Klin, M., Wi\u015bniewska, K., Lata\u0142a, A., and Wiktor, J.M. (2021). Changes in growth, photosynthesis performance, pigments, and toxin contents of bloom-forming cyanobacteria after exposure to macroalgal allelochemicals. Toxins, 13.","DOI":"10.3390\/toxins13080589"},{"key":"ref_41","first-page":"831","article-title":"Effects of allelochemical gramine on photosynthetic pigments of cyanobacterium Microcystis aeruginosa","volume":"47","author":"Hong","year":"2010","journal-title":"World Acad. Sci. Eng. Technol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.ecoenv.2019.03.097","article-title":"Understanding the inhibitory mechanism of antialgal allelochemical flavonoids from genetic variations: Photosynthesis, toxin synthesis and nutrient utility","volume":"177","author":"Yu","year":"2019","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1017\/jns.2016.41","article-title":"Flavonoids: An overview","volume":"5","author":"Panche","year":"2016","journal-title":"J. Nutr. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s10886-013-0248-5","article-title":"Flavonoids: Their structure, biosynthesis and role in the rhizosphere, including allelopathy","volume":"39","author":"Weston","year":"2013","journal-title":"J. Chem. Ecol."},{"key":"ref_45","first-page":"1","article-title":"Study on inhibitory effects of flavonoids on Microcystis aeruginosa","volume":"45","author":"Zhao","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"22389","DOI":"10.1007\/s11356-019-05507-1","article-title":"Identification of allelochemicals from pomegranate peel and their effects on Microcystis aeruginosa growth","volume":"26","author":"Chen","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Zhao, Q., Jiang, R., Shi, Y., Shen, A., He, P., and Shao, L. (2023). Allelopathic inhibition and mechanism of quercetin on Microcystis aeruginosa. Plants, 12.","DOI":"10.3390\/plants12091808"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Li, J., Cao, L., Guo, Z., An, G., Li, B., and Li, J. (2021). Time- and dose-dependent allelopathic effects and mechanisms of kaempferol on toxigenic Microcystis growth. Ecotoxicol. Environ. Saf., 222.","DOI":"10.1016\/j.ecoenv.2021.112508"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"66","DOI":"10.3390\/toxins7010066","article-title":"Effects of natural flavonoids on photosynthetic activity and cell integrity in Microcystis aeruginosa","volume":"7","author":"Huang","year":"2015","journal-title":"Toxins"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Xu, C., Yu, S., Hu, J., Effiong, K., Ge, Z., Tang, T., and Xiao, X. (2022). Programmed cell death process in freshwater Microcystis aeruginosa and marine Phaeocystis globosa induced by a plant derived allelochemical. Sci. Total Environ., 838.","DOI":"10.1016\/j.scitotenv.2022.156055"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Yu, S., Xu, C., Tang, T., Zhang, Y., Effiong, K., Hu, J., Bi, Y., and Xiao, X. (2023). Down-regulation of iron\/zinc ion transport and toxin synthesis in Microcystis aeruginosa exposed to 5,4\u2032-dihydroxyflavone. J. Hazard. Mater., 460.","DOI":"10.1016\/j.jhazmat.2023.132396"},{"key":"ref_52","first-page":"304","article-title":"Inhibitory effect of salicylic acid on Cladophora growth","volume":"16","author":"Xiong","year":"2021","journal-title":"Asian J. Ecotoxicol."},{"key":"ref_53","first-page":"169","article-title":"Allelopathic inhibition of salicylic acid on Microcystis aeruginosa","volume":"48","author":"Hu","year":"2017","journal-title":"J. South. Agric."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Fan, Z., Wang, Y., Chen, C., Li, J., He, Y., and Xiao, H. (2022). Algal inhibiting effects of salicylic acid sustained-release microspheres on algae in different growth cycles. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph19106320"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"24798","DOI":"10.1007\/s11356-018-2536-7","article-title":"Antialgal effects of \u03b1-linolenic acid on harmful bloom-forming Prorocentrum donghaiense and the antialgal mechanisms","volume":"25","author":"Wang","year":"2018","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_56","first-page":"239","article-title":"Effect of azelaic acid of mangrove root exudates on Prorocentrum micans","volume":"35","author":"Liu","year":"2013","journal-title":"Acta Oceanol. Sin."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/9\/1141\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:35:29Z","timestamp":1760034929000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/9\/1141"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,29]]},"references-count":56,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["biology14091141"],"URL":"https:\/\/doi.org\/10.3390\/biology14091141","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,29]]}}}