{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:34:40Z","timestamp":1787027680020,"version":"build-2736575974"},"reference-count":67,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019286","name":"Deanship of Research and Graduate Studies (DRG)","doi-asserted-by":"publisher","award":["2024-IRG-MED-1"],"award-info":[{"award-number":["2024-IRG-MED-1"]}],"id":[{"id":"10.13039\/501100019286","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Marine-derived Actinomycetota have emerged as promising sources of bioactive natural products, particularly filamentous actinomycetes (e.g., Streptomyces). However, members from non-filamentous genera have showed potential biotechnological importance. In this study, we performed a comprehensive genomic characterization of two bioactive Brevibacterium strains, Brevibacterium luteolum (B. luteolum) 26C and Brevibacterium casei (B. casei) 13A, isolated from two Red Sea sponges. Whole-genome sequencing and taxonomic analysis confirmed species-level identification, marking the first documented report of these species within the Red Sea ecosystem. The two strains displayed antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Candida albicans. Additionally, functional annotation revealed multiple genomic islands (GIs) enriched with genes conferring heavy metal resistance, DNA repair enzymes, nutrient acquisition, and mobile genetic elements, highlighting potential evolutionary adaptations to the harsh physicochemical conditions of the Red Sea. Genome mining identified biosynthetic gene clusters, including those encoding \u03b5-poly-L-lysine, tropodithietic acid, ectoine, and carotenoids. The comparative analysis of orthologous gene clusters from both strains and their counterparts from terrestrial ecosystems highlighted potential marine adaptive genetic mechanisms. This study highlights the biosynthetic potential of B. luteolum 26C and B. casei 13A and their ecological role as active competitors and potential defensive associates within the sponge microbiome.<\/jats:p>","DOI":"10.3390\/biology14091271","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T16:27:33Z","timestamp":1757953653000},"page":"1271","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comprehensive Genome Analysis of Two Bioactive Brevibacterium Strains Isolated from Marine Sponges from the Red Sea"],"prefix":"10.3390","volume":"14","author":[{"given":"Yehia S.","family":"Mohamed","sequence":"first","affiliation":[{"name":"Department of Pathological Sciences, College of Medicine, Ajman University, Ajman P.O. Box 346, United Arab Emirates"},{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samar M.","family":"Solyman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Sinai University\u2014Kantara Branche, Ismailia 41522, Egypt"},{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2802-2901","authenticated-orcid":false,"given":"Abdelrahman M.","family":"Sedeek","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Galala University, New Galala City 43511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8936-5011","authenticated-orcid":false,"given":"Hasnaa L.","family":"Kamel","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Sinai University\u2014Kantara Branche, Ismailia 41522, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manar","family":"El Samak","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Rasul, N.M.A., and Stewart, I.C.F. (2015). The Red Sea: The Formation, Morphology, Oceanography and Environment of a Young Ocean Basin, Springer.","DOI":"10.1007\/978-3-662-45201-1"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"fiw002","DOI":"10.1093\/femsec\/fiw002","article-title":"Biodiversity Patterns of Plankton Assemblages at the Extremes of the Red Sea","volume":"92","author":"Pearman","year":"2016","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"11870","DOI":"10.1038\/ncomms11870","article-title":"Diversity, Structure and Convergent Evolution of the Global Sponge Microbiome","volume":"7","author":"Thomas","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1038\/nrmicro2839","article-title":"Genomic Insights into the Marine Sponge Microbiome","volume":"10","author":"Hentschel","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s40168-018-0428-1","article-title":"The Sponge Holobiont in a Changing Ocean: From Microbes to Ecosystems","volume":"6","author":"Pita","year":"2018","journal-title":"Microbiome"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1128\/MMBR.00040-06","article-title":"Sponge-Associated Microorganisms: Evolution, Ecology, and Biotechnological Potential","volume":"71","author":"Taylor","year":"2007","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"86","DOI":"10.2174\/138920109787048625","article-title":"Marine Sponges: Potential Sources of New Antimicrobial Drugs","volume":"10","author":"Laport","year":"2009","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Sedeek, A.M., Elfeky, H., Hanora, A.S., and Solyman, S.M. (2025). Genomic Insights into Biosynthesis and Adaptation in the Bioactive Marine Bacterium Streptomyces albidoflavus VIP-1 from the Red Sea. BMC Microbiol., 25.","DOI":"10.1186\/s12866-025-04109-x"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100920","DOI":"10.1016\/j.margen.2021.100920","article-title":"Metataxonomic, Bioactivity and Microbiome Analysis of Red Sea Marine Sponges from Egypt","volume":"61","author":"Kamel","year":"2022","journal-title":"Mar. Genom."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"El Samak, M., Lotfy, H., Sedeek, A.M., Mohamed, Y.S., and Solyman, S.M. (2025). Genomic Characterization of Marine Staphylococcus shinii strain SC-M1C: Potential Genetic Adaptations and Ecological Role. Microorganisms, 13.","DOI":"10.3390\/microorganisms13081866"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Liang, J., She, J., Fu, J., Wang, J., Ye, Y., Yang, B., Liu, Y., Zhou, X., and Tao, H. (2023). Advances in Natural Products from the Marine-Sponge-Associated Microorganisms with Antimicrobial Activity in the Last Decade. Mar. Drugs, 21.","DOI":"10.3390\/md21040236"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"100383","DOI":"10.1016\/j.microb.2025.100383","article-title":"Marine Actinomycetes: A Promising Source of Novel Therapeutics and Pharmaceutical Bioactive Compounds\u2014A Review","volume":"7","author":"Ibrahim","year":"2025","journal-title":"Microbe"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1099\/ijs.0.65099-0","article-title":"Brevibacterium marinum sp. Nov., Isolated from Seawater","volume":"58","author":"Lee","year":"2008","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1099\/ijs.0.64869-0","article-title":"Brevibacterium oceani sp. Nov., Isolated from Deep-Sea Sediment of the Chagos Trench, Indian Ocean","volume":"58","author":"Bhadra","year":"2008","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"112656","DOI":"10.1016\/j.marpolbul.2021.112656","article-title":"Nickel Bioaccumulation by a Marine Bacterium Brevibacterium sp. (X6) Isolated from Shenzhen Bay, China","volume":"170","author":"Wu","year":"2021","journal-title":"Mar. Pollut. Bull."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1007\/s42770-020-00236-x","article-title":"Draft Whole-Genome Sequence of Brevibacterium casei strain Isolated from a Bloodstream Infection","volume":"51","author":"Olender","year":"2020","journal-title":"Braz. J. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Cumsille, A., Serna-Cardona, N., Gonz\u00e1lez, V., Claver\u00edas, F., Undabarrena, A., Molina, V., Salv\u00e0-Serra, F., Moore, E.R.B., and C\u00e1mara, B. (2023). Exploring the Biosynthetic Gene Clusters in Brevibacterium: A Comparative Genomic Analysis of Diversity and Distribution. BMC Genom., 24.","DOI":"10.1186\/s12864-023-09694-7"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A Flexible Trimmer for Illumina Sequence Data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wick, R.R., Judd, L.M., Gorrie, C.L., and Holt, K.E. (2017). Unicycler: Resolving Bacterial Genome Assemblies from Short and Long Sequencing Reads. PLoS Comput. Biol., 13.","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5315","DOI":"10.1093\/bioinformatics\/btac672","article-title":"GTDB-Tk v2: Memory friendly classification with the genome taxonomy database","volume":"38","author":"Chaumeil","year":"2022","journal-title":"Bioinformatics"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1038\/s41467-019-10210-3","article-title":"TYGS Is an Automated High-Throughput Platform for State-of-the-Art Genome-Based Taxonomy","volume":"10","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1093\/bioinformatics\/btv681","article-title":"JSpeciesWS: A Web Server for Prokaryotic Species Circumscription Based on Pairwise Genome Comparison","volume":"32","author":"Richter","year":"2016","journal-title":"Bioinformatics"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Meier-Kolthoff, J.P., Auch, A.F., Klenk, H.P., and G\u00f6ker, M. (2013). Genome Sequence-Based Species Delimitation with Confidence Intervals and Improved Distance Functions. BMC Bioinform., 14.","DOI":"10.1186\/1471-2105-14-60"},{"key":"ref_24","first-page":"e1900v1","article-title":"The Enveomics Collection: A Toolbox for Specialized Analyses of Microbial Genomes and Metagenomes","volume":"4","author":"Konstantinidis","year":"2016","journal-title":"PeerJ Prepr."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Alonge, M., Soyk, S., Ramakrishnan, S., Wang, X., Goodwin, S., Sedlazeck, F.J., Lippman, Z.B., and Schatz, M.C. (2019). RaGOO: Fast and Accurate Reference-Guided Scaffolding of Draft Genomes. Genome Biol., 20.","DOI":"10.1186\/s13059-019-1829-6"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Aziz, R.K., Bartels, D., Best, A., DeJongh, M., Disz, T., Edwards, R.A., Formsma, K., Gerdes, S., Glass, E.M., and Kubal, M. (2008). The RAST Server: Rapid Annotations Using Subsystems Technology. BMC Genom., 9.","DOI":"10.1186\/1471-2164-9-75"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: Rapid Prokaryotic Genome Annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e0099122","DOI":"10.1128\/aem.00991-22","article-title":"mobileOG-Db: A Manually Curated Database of Protein Families Mediating the Life Cycle of Bacterial Mobile Genetic Elements","volume":"88","author":"Brown","year":"2022","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"W30","DOI":"10.1093\/nar\/gkx343","article-title":"IslandViewer 4: Expanded Prediction of Genomic Islands for Larger-Scale Datasets","volume":"45","author":"Bertelli","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.jmb.2015.11.006","article-title":"BlastKOALA and GhostKOALA: KEGG Tools for Functional Characterization of Genome and Metagenome Sequences","volume":"428","author":"Kanehisa","year":"2016","journal-title":"J. Mol. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"W32","DOI":"10.1093\/nar\/gkaf334","article-title":"antiSMASH 8.0: Extended Gene Cluster Detection Capabilities and Analyses of Chemistry, Enzymology, and Regulation","volume":"53","author":"Blin","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"W397","DOI":"10.1093\/nar\/gkad313","article-title":"OrthoVenn3: An Integrated Platform for Exploring and Visualizing Orthologous Data across Genomes","volume":"51","author":"Sun","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1111\/j.1365-294X.2011.05378.x","article-title":"Biogeography of Pelagic Bacterioplankton Across an Antagonistic Temperature\u2014Salinity Gradient in the Red Sea","volume":"21","author":"Ngugi","year":"2012","journal-title":"Mol. Ecol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/nature24621","article-title":"A Communal Catalogue Reveals Earth\u2019s Multiscale Microbial Diversity","volume":"551","author":"Thompson","year":"2017","journal-title":"Nature"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1007\/s10529-005-2878-3","article-title":"Industrial Importance of the Genus Brevibacterium","volume":"27","author":"Onraedt","year":"2005","journal-title":"Biotechnol. Lett."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"133","DOI":"10.12816\/0008227","article-title":"Bioactive Crude Extracts from Four Bacterial Isolates of Marine Sediments from Red Sea, Gulf of Aqaba, Jordan","volume":"7","year":"2014","journal-title":"Jordan J. Biol. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1186\/s12934-025-02759-0","article-title":"Screening of Red Sea- and Mediterranean Sea-Derived Actinomycetes for Antimicrobial and Antitumor Activities: LC-ESI-HRMS-Based Metabolomics Study","volume":"24","author":"Azmy","year":"2025","journal-title":"Microb. Cell Fact."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"101032","DOI":"10.1016\/j.margen.2023.101032","article-title":"Metagenomic and Metatranscriptomic Exploration of the Egyptian Red Sea Sponge Theonella sp. Associated Microbial Community","volume":"70","author":"Zakeer","year":"2023","journal-title":"Mar. Genom."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1111\/lam.13728","article-title":"Recent Methods for Discovering Novel Bioactive Metabolites, Specifically Antimicrobial Agents, from Marine-Associated Micro-Organisms","volume":"75","author":"Sedeek","year":"2022","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.1574-6976.2008.00136.x","article-title":"Genomic Islands: Tools of Bacterial Horizontal Gene Transfer and Evolution","volume":"33","author":"Juhas","year":"2009","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"8402","DOI":"10.1093\/nar\/gkad644","article-title":"A Systematic Approach to Classify and Characterize Genomic Islands Driven by Conjugative Mobility Using Protein Signatures","volume":"51","author":"Audrey","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Ravcheev, D.A., Best, A.A., Sernova, N.V., Kazanov, M.D., Novichkov, P.S., and Rodionov, D.A. (2013). Genomic Reconstruction of Transcriptional Regulatory Networks in Lactic Acid Bacteria. BMC Genom., 14.","DOI":"10.1186\/1471-2164-14-94"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1128\/MMBR.00031-07","article-title":"Structure, Function, and Evolution of Bacterial ATP-Binding Cassette Systems","volume":"72","author":"Davidson","year":"2008","journal-title":"Microbiol. Mol. Biol. Rev. MMBR"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1007\/s002039900127","article-title":"Mechanism, Regulation, and Ecological Role of Bacterial Cyanide Biosynthesis","volume":"173","author":"Blumer","year":"2000","journal-title":"Arch. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1128\/MMBR.00008-08","article-title":"Biosynthesis and Functions of Mycothiol, the Unique Protective Thiol of Actinobacteria","volume":"72","author":"Newton","year":"2008","journal-title":"Microbiol. Mol. Biol. Rev. MMBR"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S0168-6445(03)00048-2","article-title":"Efflux-Mediated Heavy Metal Resistance in Prokaryotes","volume":"27","author":"Nies","year":"2003","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"23654","DOI":"10.1074\/jbc.M402823200","article-title":"Structural basis of Redox-coupled protein substrate selection by the cytochrome c biosynthesis protein ResA","volume":"279","author":"Crow","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1146\/annurev-genet-102209-163602","article-title":"The RecQ DNA Helicases in DNA Repair","volume":"44","author":"Bernstein","year":"2010","journal-title":"Annu. Rev. Genet."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1038\/nrc1012","article-title":"RecQ Helicases: Caretakers of the Genome","volume":"3","author":"Hickson","year":"2003","journal-title":"Nat. Rev. Cancer"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e16668","DOI":"10.1111\/1462-2920.16668","article-title":"From Ubiquity to Specificity: The Diverse Functions of Bacterial Thioredoxin Systems","volume":"26","author":"Anjou","year":"2024","journal-title":"Environ. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e00172-20","DOI":"10.1128\/JB.00172-20","article-title":"The Adaptive Response to Long-Term Nitrogen Starvation in Escherichia coli Requires the Breakdown of Allantoin","volume":"202","author":"Switzer","year":"2020","journal-title":"J. Bacteriol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e00133-12","DOI":"10.1128\/mBio.00133-12","article-title":"Proteomic and Transcriptomic Analyses of \u201cCandidatus Pelagibacter ubique\u201d Describe the First PII-Independent Response to Nitrogen Limitation in a Free-Living Alphaproteobacterium","volume":"4","author":"Smith","year":"2013","journal-title":"mBio"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Kalia, V.C. (2015). Book Review: Quorum Sensing vs. Quorum Quenching: A Battle with no End in Sight, Springer.","DOI":"10.1007\/978-81-322-1982-8"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.3389\/fmicb.2020.01935","article-title":"Expression Profile of Multidrug Resistance Efflux Pumps During Intracellular Life of Adherent-Invasive Escherichia coli strain LF82","volume":"11","author":"Fanelli","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S1002-0160(21)60058-9","article-title":"Hydrogen Cyanide Production by Soil Bacteria: Biological Control of Pests and Promotion of Plant Growth in Sustainable Agriculture","volume":"32","author":"Sehrawat","year":"2022","journal-title":"Pedosphere"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Flury, P., Vesga, P., P\u00e9chy-Tarr, M., Aellen, N., Dennert, F., Hofer, N., Kupferschmied, K.P., Kupferschmied, P., Metla, Z., and Ma, Z. (2017). Antimicrobial and Insecticidal: Cyclic Lipopeptides and Hydrogen Cyanide Produced by Plant-Beneficial Pseudomonas strains CHA0, CMR12a, and PCL1391 Contribute to Insect Killing. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00100"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1139\/w2012-004","article-title":"Hydrogen Cyanide Synthesis and Antifungal Activity of the Biocontrol Strain Pseudomonas fluorescens In5 from Greenland Is Highly Dependent on Growth Medium","volume":"58","author":"Michelsen","year":"2012","journal-title":"Can. J. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1038\/nrmicro1235","article-title":"Mobile Genetic Elements: The Agents of Open Source Evolution","volume":"3","author":"Frost","year":"2005","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"129513","DOI":"10.1016\/j.ijbiomac.2024.129513","article-title":"Design and Optimization of \u03b5-Poly-l-Lysine with Specific Functions for Diverse Applications","volume":"262","author":"Wang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"7758","DOI":"10.1128\/AEM.02204-14","article-title":"The Antimicrobial Mechanism of Action of Epsilon-Poly-L-Lysine","volume":"80","author":"Hyldgaard","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1128\/AEM.68.2.772-783.2002","article-title":"Osmotically Regulated Synthesis of the Compatible Solute Ectoine in Bacillus Pasteurii and Related Bacillus spp.","volume":"68","author":"Kuhlmann","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S0168-6445(03)00046-9","article-title":"Bacterial Mercury Resistance from Atoms to Ecosystems","volume":"27","author":"Barkay","year":"2003","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1093\/jac\/dkw382","article-title":"Fosfomycin Resistance in Acinetobacter baumannii Is Mediated by Efflux Through a Major Facilitator Superfamily (MFS) Transporter\u2014AbaF","volume":"72","author":"Sharma","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"9066","DOI":"10.1128\/JB.01045-07","article-title":"Regulation of Multidrug Efflux Systems Involved in Multidrug and Metal Resistance of Salmonella enterica Serovar Typhimurium","volume":"189","author":"Nishino","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1111\/1574-6976.12067","article-title":"Bacterial Insertion Sequences: Their Genomic Impact and Diversity","volume":"38","author":"Siguier","year":"2014","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/s00284-022-02810-y","article-title":"Bacterial Arsenic Metabolism and Its Role in Arsenic Bioremediation","volume":"79","author":"Kabiraj","year":"2022","journal-title":"Curr. Microbiol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"D298","DOI":"10.1093\/nar\/gku1046","article-title":"REBASE\u2014A Database for DNA Restriction and Modification: Enzymes, Genes and Genomes","volume":"43","author":"Roberts","year":"2015","journal-title":"Nucleic Acids Res."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/9\/1271\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:45:58Z","timestamp":1760035558000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/9\/1271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,15]]},"references-count":67,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["biology14091271"],"URL":"https:\/\/doi.org\/10.3390\/biology14091271","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,15]]}}}