{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T10:36:36Z","timestamp":1762425396142,"version":"build-2065373602"},"reference-count":71,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Roc\u00edo Montoya-P\u00e9rez","award":["UMSNH-CIC-2023"],"award-info":[{"award-number":["UMSNH-CIC-2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Muscle fatigue, defined as a decline in force generation, is closely linked to redox imbalance\u2014a condition exacerbated by oxidative stress in hypertension. This study investigated the effects of diazoxide administration and moderate-intensity exercise on skeletal muscle redox status following a fatigue protocol in rats with hypertension. Animals were assigned to eight groups: control (CTRL), diazoxide (DZX), exercise (EX), exercise + diazoxide (EX+DZX), hypertension (HTN), hypertension + diazoxide (HTN+DZX), hypertension + exercise (HTN+EX), and hypertension + exercise + diazoxide (HTN+ EX+DZX). Hypertension was induced by a high-salt diet. Diazoxide was administered daily for 14 days, and exercise consisted of moderate treadmill running for 8 weeks. Muscle fatigue was evoked in the extensor digitorum longus (EDL) and soleus by repetitive electrical stimulation. Post-fatigue analyses included oxidant levels, catalase activity, and glutathione status. Hypertension increased oxidants and reduced antioxidant defenses in both muscle types. Exercise and diazoxide, alone or in combination, improved redox balance, with the combined treatment providing the most robust protection and exhibiting fiber-specific adaptations. These findings suggest that diazoxide combined with moderate exercise represents a promising therapeutic approach to counteract oxidative stress-related skeletal muscle dysfunction in hypertension.<\/jats:p>","DOI":"10.3390\/biology14111553","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T16:09:47Z","timestamp":1762358987000},"page":"1553","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Combined Effects of Diazoxide and Moderate-Intensity Exercise on the Restoration of Redox Balance Post-Fatigue in Fast- and Slow-Twitch Skeletal Muscles of Hypertensive Rats"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8012-6126","authenticated-orcid":false,"given":"Estefan\u00eda","family":"Bravo-S\u00e1nchez","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana San Nicol\u00e1s de Hidalgo, Morelia 58040, Michoac\u00e1n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar J.","family":"Nolasco-Ruiz","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana San Nicol\u00e1s de Hidalgo, Morelia 58040, Michoac\u00e1n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7042-7825","authenticated-orcid":false,"given":"Sarai","family":"S\u00e1nchez-Duarte","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana San Nicol\u00e1s de Hidalgo, Morelia 58040, Michoac\u00e1n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana","family":"G\u00f3mez-Barroso","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana San Nicol\u00e1s de Hidalgo, Morelia 58040, Michoac\u00e1n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3239-123X","authenticated-orcid":false,"given":"Manuel Alejandro","family":"Vargas-Vargas","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana San Nicol\u00e1s de Hidalgo, Morelia 58040, Michoac\u00e1n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4850-772X","authenticated-orcid":false,"given":"Christian","family":"Cort\u00e9s-Rojo","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana San Nicol\u00e1s de Hidalgo, Morelia 58040, Michoac\u00e1n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvador","family":"Manzo-\u00c1valos","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana San Nicol\u00e1s de Hidalgo, Morelia 58040, Michoac\u00e1n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7089-4375","authenticated-orcid":false,"given":"Elizabeth","family":"S\u00e1nchez-Duarte","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Aplicadas al Trabajo, Universidad Guanajuato Campus Le\u00f3n, Le\u00f3n 37128, Guanajuato, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1401-6535","authenticated-orcid":false,"given":"Alain Raimundo","family":"Rodr\u00edguez-Orozco","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias M\u00e9dicas y Biol\u00f3gicas \u201cDr. Ignacio Ch\u00e1vez\u201d, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Morelia 58000, Michoac\u00e1n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0811-2950","authenticated-orcid":false,"given":"Alfredo","family":"Saavedra-Molina","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana San Nicol\u00e1s de Hidalgo, Morelia 58040, Michoac\u00e1n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7569-1358","authenticated-orcid":false,"given":"Roc\u00edo","family":"Montoya-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana San Nicol\u00e1s de Hidalgo, Morelia 58040, Michoac\u00e1n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Constantin-Teodosiu, D., and Constantin, D. 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