{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T15:01:05Z","timestamp":1784818865208,"version":"3.55.0"},"reference-count":36,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T00:00:00Z","timestamp":1763164800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>A total of 304 bacterial strains were isolated from the Taklamakan Desert. Through screening, strain TRM82367 exhibited potent insecticidal activity against cotton aphids (Aphis gossypii) and was identified as Bacillus velezensis. To explore the primary insecticidal active components of this strain, lipopeptide substances in the fermentation broth were extracted via hydrochloric acid precipitation. The main active components were analyzed using activity-guided fractionation combined with liquid chromatography\u2013mass spectrometry (LC-MS). Our results demonstrated that Bacillus velezensis TRM82367 possessed strong lethal activity against cotton aphids. The crude extract obtained by means of hydrochloric acid precipitation and methanol dissolution showed high insecticidal efficacy. At concentrations of 100, 150, 200, 250, 300, 400, and 500 mg\/L, the 48 h corrected mortality rates of cotton aphids were 25.14%, 38.05%, 50.09%, 53.03%, 57.96%, 74.68%, and 88.67%, respectively. The toxicity regression equation was Y = 2.47X \u2212 5.72, with a median lethal concentration (LC50) of 207.616 mg\/L and a 95% lethal concentration (LC95) of 1004.673 mg\/L. After separation by an ODS chromatographic column and identification by means of mass spectrometry, the main active components were confirmed to be C12\u2013C16 surfactin homologs.<\/jats:p>","DOI":"10.3390\/biology14111598","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T14:04:07Z","timestamp":1763388247000},"page":"1598","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigation into the Active Substance of Bacillus velezensis TRM82367 for Killing Aphis gossypii"],"prefix":"10.3390","volume":"14","author":[{"given":"Shiyu","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Science and Technology, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Science and Technology, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Wen","sequence":"additional","affiliation":[{"name":"State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Science and Technology, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3627-943X","authenticated-orcid":false,"given":"Zhanfeng","family":"Xia","sequence":"additional","affiliation":[{"name":"State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Science and Technology, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Cocuzza, G. (2024). Aphis Gossypii (Cotton Aphid). CABI Compend., 6204.","DOI":"10.1079\/cabicompendium.6204"},{"key":"ref_2","first-page":"1-6+9","article-title":"The 2024 Annual Cotton Market Analysis and the Situation Outlook in 2025","volume":"52","author":"Zhai","year":"2025","journal-title":"China Cotton"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Xu, X., Shi, J., Chen, Y., He, Q., Liu, L., Sun, T., Ding, R., Lu, Y., Xue, C., and Qiao, H. (2023). Research on Machine Vision and Deep Learning Based Recognition of Cotton Seedling Aphid Infestation Level. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1200901"},{"key":"ref_4","first-page":"277","article-title":"Species Diversity and Distribution Characteristics of Aphid Insects in China (Hemiptera, Aphid Superfamily)","volume":"34","author":"Liu","year":"2009","journal-title":"J. Anim. Taxon."},{"key":"ref_5","first-page":"222","article-title":"The causes and control methods of aphids","volume":"240","author":"Wang","year":"2007","journal-title":"Anhui Agric. Sci. Bull."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.pestbp.2016.02.005","article-title":"Cycloxaprid: A Novel Cis-Nitromethylene Neonicotinoid Insecticide to Control Imidacloprid-Resistant Cotton Aphid (Aphis gossypii)","volume":"132","author":"Cui","year":"2016","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_7","unstructured":"Saimi, W., Subatijiang, S., Ma, D.Y., and Patima, W. (2023). Field Efficacy of Neonicotinoid Insecticides and Artificial Release of Adonia variegata in Controlling Cotton Aphids. Xinjiang Agric. Sci. Technol., 41\u201343."},{"key":"ref_8","first-page":"376","article-title":"Evaluation of the Occurrence of Aphids in Peanut-Cotton Intercropping Fields and Their Resistance Level to Neonicotinoid Insecticides","volume":"63","author":"Xue","year":"2024","journal-title":"Pesticides"},{"key":"ref_9","first-page":"78","article-title":"Analysis of the Main Influencing Factors of Drift in Chemical Pesticide Spraying","volume":"37","author":"Wang","year":"2016","journal-title":"J. Chin. Agric. Mech."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"108653","DOI":"10.1016\/j.envint.2024.108653","article-title":"Assessing Pesticides in the Atmosphere: A Global Study on Pollution, Human Health Effects, Monitoring Network and Regulatory Performance","volume":"187","author":"Huang","year":"2024","journal-title":"Environ. Int."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e31550","DOI":"10.1016\/j.heliyon.2024.e31550","article-title":"Bacterial Biopesticides: Biodiversity, Role in Pest Management and Beneficial Impact on Agricultural and Environmental Sustainability","volume":"10","author":"Tomar","year":"2024","journal-title":"Heliyon"},{"key":"ref_12","first-page":"41","article-title":"Research Progress on Frontier Technologies in Bio-Agriculture","volume":"44","author":"Wei","year":"2024","journal-title":"Chin. J. Bioeng."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Masmoudi, F., Pothuvattil, N.S., Tounsi, S., Saadaoui, I., and Trigui, M. (2023). Synthesis of Silver Nanoparticles Using Bacillus velezensis M3-7 Lipopeptides: Enhanced Antifungal Activity and Potential Use as a Biocontrol Agent against Fusarium Crown Rot Disease of Wheat Seedlings. Int. J. Food Microbiol., 407.","DOI":"10.1016\/j.ijfoodmicro.2023.110420"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Kim, J.-A., Song, J.-S., Kim, P.I., Kim, D.-H., and Kim, Y. (2022). Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi. J. Fungi, 8.","DOI":"10.3390\/jof8101053"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"124827","DOI":"10.1016\/j.jhazmat.2020.124827","article-title":"A Review on New Aspects of Lipopeptide Biosurfactant: Types, Production, Properties and Its Application in the Bioremediation Process","volume":"407","author":"Kumar","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_16","unstructured":"Wen, F. (2025). Bacterial Resources and Diversity in the Central Taklimakan Desert. [Master\u2019s Thesis, Tarim University]."},{"key":"ref_17","first-page":"56","article-title":"Toxicity and Safety Evaluation of Five Biopesticides against Myzus persicae and Aphidoletes aphidimyza","volume":"41","author":"Shen","year":"2020","journal-title":"Chin. Tob. Sci."},{"key":"ref_18","unstructured":"Dong, X., and Cai, M. (2001). Manual for the Systematic Identification of Common Bacteria, Science Press."},{"key":"ref_19","first-page":"75","article-title":"An Improved DNA Extraction Method for Soil Microorganisms in Upland","volume":"49","author":"Hu","year":"2020","journal-title":"J. Henan Agric. Sci."},{"key":"ref_20","unstructured":"Xu, B. (2020). Purification, Identification and Biosynthesis Pathway Analysis of Antimicrobial Compounds Produced by Bacillus siamensis JFL15. [Ph.D. Thesis, South China Agricultural University]."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1099\/ijs.0.63310-0","article-title":"Bacillus velezensis sp. Nov., a Surfactant-Producing Bacterium Isolated from the River V\u00e9lez in M\u00e1laga, Southern Spain","volume":"55","author":"Llamas","year":"2005","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"541","DOI":"10.2174\/138620703106298716","article-title":"Diversity among Microbial Cyclic Lipopeptides: Iturins and Surfactins. Activity-Structure Relationships to Design New Bioactive Agents","volume":"6","author":"Bonmatin","year":"2003","journal-title":"Comb. Chem. High Throughput Screen."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"De Castro, M.T., Ferreira, A.D.C.D.L., Do Nascimento, I.N., Rocha, G.T., Freire, \u00cd.A., Gomes, G.C., Dos Reis Cunha, B.B., Linhares Montalv\u00e3o, S.C., Moreira, F.M., and Da Costa, R.A. (2025). Bacillus velezensis SBB80 Affects the Development of Fusarium and Neocosmospora Species and Controls Diatraea Saccharalis (Lepidoptera: Crambidae) Larvae. Biol. Control, 210.","DOI":"10.1016\/j.biocontrol.2025.105888"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1007\/s00216-015-8486-8","article-title":"Identification of Lipopeptide Isoforms by MALDI-TOF-MS\/MS Based on the Simultaneous Purification of Iturin, Fengycin, and Surfactin by RP-HPLC","volume":"407","author":"Yang","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.3390\/md8102605","article-title":"Characterization and Online Detection of Surfactin Isomers Based on HPLC-MSn Analyses and Their Inhibitory Effects on the Overproduction of Nitric Oxide and the Release of TNF-\u03b1 and IL-6 in LPS-Induced Macrophages","volume":"8","author":"Tang","year":"2010","journal-title":"Mar. Drugs"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4747","DOI":"10.1007\/s00253-017-8272-y","article-title":"Direct Quantification of Lipopeptide Biosurfactants in Biological Samples via HPLC and UPLC-MS Requires Sample Modification with an Organic Solvent","volume":"101","author":"Biniarz","year":"2017","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_27","first-page":"177","article-title":"Purification, Identification, and Characterization of Surfactin from Bacillus velezensis 1-3","volume":"44","author":"Zhang","year":"2023","journal-title":"Food Sci."},{"key":"ref_28","first-page":"35","article-title":"Identification of Fusarium oxysporum inhibitory peptides from Bacillus velezensis","volume":"40","author":"Guo","year":"2021","journal-title":"China Brew."},{"key":"ref_29","unstructured":"Yu, F. (2025). Study on the Antibacterial Properties of Surface Active Substances in Bacillus velesiensis ya215 and the Regulatory Mechanism of Branched-Chain Amino Acids on Its Synthesis. [Ph.D. Thesis, Guangxi University]."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Tang, T., Wang, F., Huang, H., Guo, J., Guo, X., Duan, Y., Wang, X., Wang, Q., and You, J. (2024). Bacillus velezensis LT1: A Potential Biocontrol Agent for Southern Blight on Coptis Chinensis. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1337655"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wang, Z., Zhao, Y., Shao, J., Wang, J., Xun, W., Sun, X., Xu, Z., Miao, Y., Huang, G., and Liu, D. (2025). Rhizosphere Domestication Enhances Root Colonization and Plant Growth Promotion Performance of Bacillus velezensis SQR9. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1638130"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Wang, Y., Sun, Z., Wang, S., Wen, F., and Xia, Z. (2025). Surfactin: A Novel Aphis gossypii Killing Surfactin Produced by Bacillus australimaris TRM82479 of Taklamakan Desert Origin. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1559495"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/s00232-019-00067-4","article-title":"Role of Lipid Composition, Physicochemical Interactions, and Membrane Mechanics in the Molecular Actions of Microbial Cyclic Lipopeptides","volume":"252","author":"Balleza","year":"2019","journal-title":"J. Membr. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.jip.2016.10.001","article-title":"Insecticidal Spectrum and Mode of Action of the Bacillus thuringiensis Vip3Ca Insecticidal Protein","volume":"142","author":"Ceballos","year":"2017","journal-title":"J. Invertebr. Pathol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.jip.2017.07.002","article-title":"Specificity and Putative Mode of Action of a Mosquito Larvicidal Toxin from the Bacterium xenorhabdus Innexi","volume":"149","author":"Kim","year":"2017","journal-title":"J. Invertebr. Pathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1002\/ps.7188","article-title":"Catalyzing Systemic Movement of Inward Rectifier Potassium Channel Inhibitors for Antifeedant Activity against the Cotton Aphid, Aphis gossypii (Glover)","volume":"79","author":"Liu","year":"2023","journal-title":"Pest Manag. Sci."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/11\/1598\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T05:13:28Z","timestamp":1763529208000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/11\/1598"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,15]]},"references-count":36,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["biology14111598"],"URL":"https:\/\/doi.org\/10.3390\/biology14111598","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,15]]}}}