{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T06:03:50Z","timestamp":1784873030196,"version":"3.55.0"},"reference-count":54,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education and Science of Ukrain","award":["0124U000567"],"award-info":[{"award-number":["0124U000567"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Synthetic anthelmintic drugs not only contribute to the development of pathogen resistance and environmental pollution but also to the development of pathogen resistance. Therefore, identifying the anthelmintic properties of widely used medicinal plants could be of great practical interest to veterinary and human medicine. In our experiment, we evaluated the in vitro survivability of the noninvasive and invasive (L1\u20132 and L3, respectively) larvae of Strongyloides papillosus and Haemonchus contortus subject to aqueous solutions of ethanolic tinctures of traditional medicinal plants (46 species). Most of the plant species we studied belonged to the families Asteraceae (Achillea millefolium, Arctium lappa, Artemisia absinthium, Bidens tripartita, Calendula officinalis, Cynara cardunculus var. scolymus, Echinacea purpurea, Helichrysum arenarium, Inula helenium, Matricaria chamomilla, Silybum marianum, Tanacetum vulgare, Taraxacum officinale, Tragopogon porrifolius), Rosaceae (Agrimonia eupatoria, Fragaria vesca, Sanguisorba officinalis), and Lamiaceae (Leonurus cardiaca, Mentha \u00d7 piperita, Origanum vulgare, Salvia officinalis, Thymus vulgaris). Other plant families were represented by 1\u20133 species: Fabaceae (Glycyrrhiza glabra, Hedysarum alpinum, Trifolium pratense), Salicaceae (Populus nigra, P. tremula, Salix alba), Fagaceae (Quercus robur), Betulaceae (Betula pendula), Juglandaceae (Juglans regia), Rhamnaceae (Frangula alnus), Acoraceae (Acorus calamus), Apiaceae (Foeniculum vulgare), Caprifoliaceae (Valeriana officinalis), Cucurbitaceae (Cucurbita pepo), Equisetaceae (Equisetum arvense), Ericaceae (Vaccinium vitis-idaea), Gentianaceae (Centaurium erythraea), Hypericaceae (Hypericum perforatum), Malvaceae (Althaea officinalis), Plantaginaceae (Linaria vulgaris, Plantago major), Poaceae (Zea mays), Polygonaceae (Polygonum aviculare), and Ranunculaceae (Nigella sativa). We determined Artemisia absinthium, Inula helenium, Matricaria chamomilla, Salvia officinalis, and Populus nigra, whose aqueous solutions of alcohol tinctures demonstrated nematocidal properties. The other plants we studied did not affect the viability of parasitic nematode larvae.<\/jats:p>","DOI":"10.3390\/biology14121636","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T09:50:26Z","timestamp":1763718626000},"page":"1636","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anthelmintic Activity of Traditional Medicinal Plants Used in Europe"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7299-9920","authenticated-orcid":false,"given":"Olexandra","family":"Boyko","sequence":"first","affiliation":[{"name":"Department of Parasitology and Veterinary Expertise, Examination, Faculty of Veterinary Medicine, Dnipro State Agrarian and Economic University, Sergiy Efremov St., 25, 49000 Dnipro, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9448-8232","authenticated-orcid":false,"given":"Viktor","family":"Brygadyrenko","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Ecology, Faculty of Biology and Ecology, Oles Honchar Dnipro National University, Nauky Av., 72, 49010 Dnipro, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,21]]},"reference":[{"key":"ref_1","first-page":"339","article-title":"Prevalence of gastrointestinal nematodes, cestodes, and protozoans of goats in Nyagatare District, Rwanda","volume":"13","author":"Tumusiime","year":"2022","journal-title":"Vet. Med. Res. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"8812142","DOI":"10.1155\/2024\/8812142","article-title":"Haemonchosis in small ruminants under traditional husbandry systems in Apac District, Northern Uganda","volume":"2024","author":"Opeto","year":"2024","journal-title":"J. Parasitol. Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Arsenopoulos, K.V., Fthenakis, G.C., Katsarou, E.I., and Papadopoulos, E. (2021). Haemonchosis: A challenging parasitic infection of sheep and goats. Animals, 11.","DOI":"10.3390\/ani11020363"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Chan, A.H.E., Kaenkaew, C., Pakdee, W., Sungpradit, S., and Thaenkham, U. (2025). Emergence of dual drug-resistant strongylids in goats: First phenotypic and genotypic evidence from Ratchaburi Province, Central Thailand. BMC Vet. Res., 21.","DOI":"10.1186\/s12917-025-04700-4"},{"key":"ref_5","first-page":"433","article-title":"The viability of Haemonchus contortus (Nematoda, Strongylida) and Strongyloides papillosus (Nematoda, Rhabditida) larvae exposed to various flavourings and source materials used in food production","volume":"53","author":"Boyko","year":"2019","journal-title":"Zoodiversity"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"85","DOI":"10.47665\/tb.38.2.046","article-title":"Nematicidal activity of aqueous tinctures of plants against larvae of the nematode Strongyloides papillosus","volume":"38","author":"Boyko","year":"2021","journal-title":"Trop. Biomed."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Boyko, O., and Brygadyrenko, V. (2022). Nematicidal activity of organic food additives. Diversity, 14.","DOI":"10.3390\/d14080615"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.exppara.2018.10.009","article-title":"Ovicidal and larvicidal activity of extracts from medicinal-plants against Haemonchus contortus","volume":"195","author":"Hodges","year":"2018","journal-title":"Exp. Parasitol."},{"key":"ref_9","first-page":"216","article-title":"Review on phytochemical constituents and pharmacological activities of plant Calendula officinalis Linn","volume":"2","author":"Dhingra","year":"2022","journal-title":"Biol. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"B\u0103ie\u015f, M.-H., Cotu\u0163iu, V.-D., Sp\u00eenu, M., Mathe, A., Cozma-Petru\u021b, A., Boc\u01cene\u0163, V.I., and Cozma, V. (2023). Satureja hortensis L. and Calendula officinalis L., two Romanian plants, with in vivo antiparasitic potential against digestive parasites of swine. Microorganisms, 11.","DOI":"10.3390\/microorganisms11122980"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"804658","DOI":"10.1155\/2015\/804658","article-title":"Chemical composition and antioxidant and antimicrobial activities of wormwood (Artemisia absinthium L.) essential oils and phenolics","volume":"2015","author":"Msaada","year":"2015","journal-title":"J. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1017\/S0022149X17000529","article-title":"Therapeutic efficacy of Artemisia absinthium against Hymenolepis nana: In vitro and in vivo studies in comparison with the anthelmintic Praziquantel","volume":"92","author":"Beshay","year":"2017","journal-title":"J. Helminthol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ozturk, M., and Hakeem, K.R. (2019). Chemical composition and biological uses of Artemisia absinthium (wormwood). Plant and Human Health. Volume 3. Pharmacology and Therapeutic Uses, Springer.","DOI":"10.1007\/978-3-030-04408-4"},{"key":"ref_14","first-page":"194","article-title":"Antiparasitic, antiprotozoal, molluscicidal and insecticidal activity of medicinal plants (Part 2)\u2014Plant based review","volume":"5","year":"2016","journal-title":"Sch. Acad. J. Pharm."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Bibi, Y., Zahara, K., Qayyum, A., and Jenks, M.A. (2025). Traditional uses and modern pharmacological investigations of Bidens frondosa and Bidens tripartita. The Genus Bidens. Chemistry and Pharmacology, Springer.","DOI":"10.1007\/978-981-96-4257-1"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.phymed.2012.02.005","article-title":"Activity of Matricaria chamomilla essential oil against anisakiasis","volume":"19","author":"Romero","year":"2012","journal-title":"Phytomedicine"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1080\/10942912.2023.2293661","article-title":"An updated comprehensive review of the therapeutic properties of chamomile (Matricaria chamomilla L.)","volume":"27","author":"Akram","year":"2023","journal-title":"Int. J. Food Prop."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.indcrop.2014.05.047","article-title":"Agrorefinery of Tanacetum vulgare L. into valuable products and evaluation of their antioxidant properties and phytochemical composition","volume":"60","author":"Venskutonis","year":"2014","journal-title":"Ind. Crops Prod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1186\/s42269-021-00567-1","article-title":"A comprehensive review of the benefits of Taraxacum officinale on human health","volume":"45","author":"Zucchetti","year":"2021","journal-title":"Bull. Natl. Res. Cent."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1007\/s11686-021-00513-1","article-title":"Dandelion (Taraxacum officinale) improves the therapeutic efficiency of Praziquantel in experimental schistosomiasis","volume":"67","author":"Atwa","year":"2022","journal-title":"Acta Parasitol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"11644","DOI":"10.1021\/acs.jafc.0c04791","article-title":"Health benefits of Silybum marianum: Phytochemistry, pharmacology, and applications","volume":"68","author":"Wang","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s41182-021-00363-7","article-title":"Silybum marianum ethanolic extract: In vitro effects on protoscolices of Echinococcus granulosus G1 strain with emphasis on other Iranian medicinal plants","volume":"49","author":"Taghipour","year":"2021","journal-title":"Trop. Med. Health"},{"key":"ref_23","first-page":"1055","article-title":"Hepatoprotective, gastroprotective, antioxidant activity and phenolic constituents of Quercus robur leaves","volume":"7","author":"Moharram","year":"2015","journal-title":"J. Pharm. Sci. Res."},{"key":"ref_24","first-page":"389","article-title":"Evaluation of the anthelmintic effects of Quercus robur extract against ovine gastrointestinal nematodes","volume":"71","author":"Fakour","year":"2016","journal-title":"J. Vet. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1007\/s11101-020-09707-3","article-title":"The polyphenolic profile of oak (Quercus) species: A phytochemical and pharmacological overview","volume":"19","author":"Renda","year":"2020","journal-title":"Phytochem. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1002\/cbdv.201400293","article-title":"Anthelmintic effects of the essential oil of fennel (Foeniculum vulgare Mill., Apiaceae) against Schistosoma mansoni","volume":"12","author":"Wakabayashi","year":"2015","journal-title":"Chem. Biodivers."},{"key":"ref_27","first-page":"3161","article-title":"Manoj phytochemical analysis and anthelmintic potential of Nigella sativa against the trematode, Cotylophoron cotylophorum","volume":"8","author":"Selvaraju","year":"2019","journal-title":"J. Pharmacogn. Phytochem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1080\/10942912.2023.2192436","article-title":"Pharmacological, nutraceutical, functional and therapeutic properties of fennel (Foeniculum vulgare)","volume":"26","author":"Noreen","year":"2023","journal-title":"Int. J. Food Prop."},{"key":"ref_29","unstructured":"Zajac, A.M., and Conboy, G.A. (2012). Veterinary Clinical Parasitology, Willey-Blackwell. [8th ed.]."},{"key":"ref_30","first-page":"277","article-title":"Morphological identifcation of nematode larvae of small ruminants and cattle simplifed","volume":"119","author":"Cabaret","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_31","first-page":"a539","article-title":"Morphological identifcation of parasitic nematode infective larvae of small ruminants and cattle: A practical lab guide","volume":"80","author":"Mayhew","year":"2013","journal-title":"Onderstepoort J. Vet. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"227","DOI":"10.15421\/011931","article-title":"Nematocidial activity of aqueous solutions of plants of the families Cupressaceae, Rosaceae, Asteraceae, Fabaceae, Cannabaceae and Apiaceae","volume":"27","author":"Boyko","year":"2019","journal-title":"Biosyst. Divers."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"85","DOI":"10.15421\/011913","article-title":"Morphological variation of four species of Strongyloides (Nematoda, Rhabditida) parasitising various mammal species","volume":"27","author":"Gugosyan","year":"2019","journal-title":"Biosyst. Divers."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1017\/S0022149X08972515","article-title":"Anthelmintic efficacy of Achillea millifolium against gastrointestinal nematodes of sheep: In vitro and in vivo studies","volume":"82","author":"Tariq","year":"2008","journal-title":"J. Helminthol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1002\/ptr.5840","article-title":"Pharmacognosy, phytochemistry and pharmacological properties of Achillea millefolium L.: A review","volume":"31","author":"Ali","year":"2017","journal-title":"Phytother. Res."},{"key":"ref_36","unstructured":"Masoodi, M.H., and Rehman, M.U. (2022). Ethnopharmacology, phytochemistry, and biological activities of Achillea millefolium: A comprehensive review. Edible Plants in Health and Diseases. Volume II: Phytochemical and Pharmacological Properties, Springer."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Shyam, M., and Sabina, E.P. (2024). Harnessing the power of Arctium lappa root: A review of its pharmacological properties and therapeutic applications. Nat. Prod. Bioprospecting, 14.","DOI":"10.1007\/s13659-024-00466-8"},{"key":"ref_38","first-page":"1030","article-title":"Anti-leishmanial effect of the hydroalcoholic extract of the leaves, roots and seeds of Arctium lappa","volume":"15","author":"Maleki","year":"2025","journal-title":"Avicenna J. Phytomed."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e147","DOI":"10.1017\/S0022149X20000310","article-title":"In vitro anthelmintic activity assessment of six medicinal plant aqueous extracts against donkey strongyles","volume":"94","author":"Buza","year":"2020","journal-title":"J. Helminthol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.vetpar.2008.10.084","article-title":"Anthelmintic activity of extracts of Artemisia absinthium against ovine nematodes","volume":"160","author":"Tariq","year":"2008","journal-title":"Vet. Parasitol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"587","DOI":"10.15421\/022482","article-title":"Antibacterial and nematicidal activities of extracts from plants of the Asteraceae family","volume":"15","author":"Zazharskyi","year":"2024","journal-title":"Regul. Mech. Biosyst."},{"key":"ref_42","first-page":"53","article-title":"Inula helenium: A literature review on ethnomedical uses, bioactive compounds and pharmacological activities","volume":"63","author":"Buza","year":"2020","journal-title":"Lucr. \u015etiin\u0163. Ser. Med. Vet."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1017\/S0022149X17000396","article-title":"Anthelmintic activity of Tunisian chamomile (Matricaria recutita L.) against Haemonchus contortus","volume":"92","author":"Hajaji","year":"2018","journal-title":"J. Helminthol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"K\u013cavi\u0146a, A., Keid\u0101ne, D., Ganola, K., L\u016bsis, I., \u0160ukele, R., Bandere, D., and Kovalcuka, L. (2023). Anthelmintic activity of Tanacetum vulgare L. (leaf and flower) extracts against Trichostrongylidae nematodes in sheep in vitro. Animals, 13.","DOI":"10.3390\/ani13132176"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"252","DOI":"10.5455\/annalsmedres.2019.07.409","article-title":"Anthelmintic effect of essential oil and extract produced from Salvia sclarea L. (Lamiaceae) on nematodes living in gastrointestinal system of sheep","volume":"27","author":"Gul","year":"2021","journal-title":"Ann. Med. Res."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"\u0160trbac, F., Krnjaji\u0107, S., Stojanovi\u0107, D., Ratajac, R., Simin, N., Or\u010di\u0107, D., Rinaldi, L., Ciccone, E., Maurelli, M.P., and Cringoli, G. (2023). In vitro and in vivo anthelmintic efficacy of peppermint (Mentha x piperita L.) essential oil against gastrointestinal nematodes of sheep. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1232570"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"\u0160trbac, F., Krnjaji\u0107, S., Maurelli, M.P., Stojanovi\u0107, D., Simin, N., Or\u010di\u0107, D., Ratajac, R., Petrovi\u0107, K., Kne\u017eevi\u0107, G., and Cringoli, G. (2023). A potential anthelmintic phytopharmacological source of Origanum vulgare (L.) essential oil against gastrointestinal nematodes of sheep. Animals, 13.","DOI":"10.3390\/ani13010045"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.vetpar.2016.08.011","article-title":"Thymus vulgaris L. essential oil and its main component thymol: Anthelmintic effects against Haemonchus contortus from sheep","volume":"228","author":"Ferreira","year":"2016","journal-title":"Vet. Parasitol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Boyko, O., and Brygadyrenko, V. (2023). Survival of nematode larvae after treatment with eugenol, isoeugenol, thymol, and carvacrol. Front. Biosci Elite Ed., 15.","DOI":"10.31083\/j.fbe1504025"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Boyko, O.O., and Brygadyrenko, V.V. (2025). Sustainability of pasture ecosystems: The use of plant-based mixtures in the fight against helminths. Biosyst. Divers., 33.","DOI":"10.15421\/012532"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Zazharskyi, V.V., Brygadyrenko, V.V., Zazharska, N.M., Boyko, O.O., Borovik, I.V., Kulishenko, O.M., and Davydenko, P.O. (2025). Antibacterial and nematocidal activities of extracts of plants of the families Poaceae, Cyperaceae, Asparagaceae, Convolvulaceae, Crassulaceae, Rosaceae, Lamiaceae, and Boraginaceae. Regul. Mech. Biosyst., 16.","DOI":"10.15421\/0225045"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1051\/parasite\/2021060","article-title":"In vitro anthelmintic activity of an aqueous extract of Glycyrrhiza glabra and of glycyrrhetinic acid against gastrointestinal nematodes of small ruminants","volume":"28","author":"Maestrini","year":"2021","journal-title":"Parasite"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1007\/s11259-024-10411-2","article-title":"Evaluation of anthelmintic efficacy of ethanolic leaf extract of Juglans regia L. on Ascaridia galli: A comprehensive in vitro and in vivo study","volume":"48","author":"Mir","year":"2024","journal-title":"Vet. Res. Commun."},{"key":"ref_54","first-page":"207","article-title":"Anthelmintic activity of Nigella sativa L., seeds on gastrointestinal nematodes of sheep","volume":"26","author":"Phulan","year":"2008","journal-title":"Pak. J. Nematol."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/12\/1636\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T10:07:53Z","timestamp":1763719673000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/14\/12\/1636"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,21]]},"references-count":54,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["biology14121636"],"URL":"https:\/\/doi.org\/10.3390\/biology14121636","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,21]]}}}