{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:31:40Z","timestamp":1790127100100,"version":"4.0.1"},"reference-count":58,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:00:00Z","timestamp":1767139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Qatar Research Development and Innovation (QRDI) Council","award":["ARG01-0420-230007"],"award-info":[{"award-number":["ARG01-0420-230007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Obesity plays a crucial role in the progression of insulin resistance and type 2 diabetes which are related to inflammation and liver disease. GATA-3 is a transcription factor that is involved in adipogenesis and inflammation. Therefore, it could be a potential therapeutic target for obesity-associated metabolic disorders. This study aimed to examine the effects of GATA-3 suppression on body weight, fat depot redistribution, liver histopathology, and inflammatory markers in transgenic db\/db obese mice. Male db\/db mice received subcutaneous injections of GATA-3-specific DNAzyme (hgd40; 10 or 100 \u00b5g\/mL), pioglitazone (as a positive control), or vehicle only (as a negative control), twice weekly for two weeks. Body weight, organ weights, liver histopathology, mRNA expression of selected genes and serum cytokine levels were assessed. GATA-3 expression was not region specific, and its suppression did not significantly affect fat depot distribution or organ weights. However, the low dose of hgd40 accelerated body weight gain transiently. It also increased Il10 mRNA expression in the liver and significantly increased IL-10 protein concentration in the serum. In addition, a high dose of hgd40 resulted in a marked decrease in hepatocyte ballooning degeneration. These findings suggest that GATA-3 suppression may modulate inflammation and liver injury in obesity, warranting further investigation into its therapeutic potential for obesity-related metabolic disorders.<\/jats:p>","DOI":"10.3390\/biology15010089","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T17:24:58Z","timestamp":1767201898000},"page":"89","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["GATA-3 Suppression by DNAzyme Modulates Interleukin-10 and Liver Injury Markers in db\/db Mice"],"prefix":"10.3390","volume":"15","author":[{"given":"Layla","family":"Al-Mansoori","sequence":"first","affiliation":[{"name":"Biomedical Research Center, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6927-5622","authenticated-orcid":false,"given":"Asma A.","family":"Elashi","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5136-9746","authenticated-orcid":false,"given":"Laila","family":"Hedaya","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3812-1310","authenticated-orcid":false,"given":"Maha","family":"Alser","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4572-9738","authenticated-orcid":false,"given":"Shamma","family":"Almuraikhy","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7263-4905","authenticated-orcid":false,"given":"Najeha","family":"Anwardeen","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9639-0773","authenticated-orcid":false,"given":"Hend","family":"Al-Jaber","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhad","family":"Hussain","sequence":"additional","affiliation":[{"name":"Biomedical Research Center, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamda A.","family":"Al-Naemi","sequence":"additional","affiliation":[{"name":"Laboratory Animal Research Centre, Qatar University, Doha P.O. Box 2713, Qatar"},{"name":"Department of Biological and Environmental Sciences, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijay","family":"Govindharajan","sequence":"additional","affiliation":[{"name":"Laboratory Animal Research Centre, Qatar University, Doha P.O. 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