{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T01:07:29Z","timestamp":1793754449639,"version":"4.6.1"},"reference-count":101,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Foodborne pathogens remain a global public health concern, and antimicrobial resistance increases their impact. In mass-gathering cities such as Al-Madinah Al-Munawarah, contaminated ready-to-eat (RTE) fast foods can contribute to both local transmission and international spread. In this study, 300 RTE fast food samples, including shawarma, burgers, fried chicken, sandwiches, and salads, were collected from international franchises, local restaurants, and street vendors. Pathogens were identified using conventional culture combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Antimicrobial susceptibility testing followed CLSI guidelines, and real-time PCR confirmed species identity and screened resistance determinants. Principal component analysis (PCA) and dendrogram clustering were used to assess diagnostic discrimination. Among the 300 samples, 129 (43.0%) were culture positive. The most common pathogens were Staphylococcus aureus (14.3%) and Escherichia coli (13.0%), followed by Salmonella spp. (9.0%) and Acinetobacter baumannii (6.7%). About 35% of S. aureus isolates were methicillin resistant (MRSA), and 85% of A. baumannii carried OXA-type carbapenemase genes. MALDI-TOF MS achieved 96.1% score-based identification and, with PCA, showed strong interspecies separation. PCR confirmed species identity and detected widespread resistance genes, with genotype\u2013phenotype concordance of at least 80%. Overall, 60.5% of isolates were multidrug resistant. RTE fast foods in Al-Madinah represent reservoirs of MDR pathogens, including carbapenemase-producing A. baumannii. The combined use of MALDI-TOF MS and real-time PCR established a rapid and scalable workflow that provided reliable identification and resistance profiling in less than 24 h, compared with 48 to 72 h for conventional methods. This approach supports One Health surveillance in high-risk food settings and strengthens preparedness for mass gatherings.<\/jats:p>","DOI":"10.3390\/biology15010104","type":"journal-article","created":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:28:57Z","timestamp":1767626937000},"page":"104","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular Profiling of Foodborne Pathogens in Ready-to-Eat Foods, Al-Madinah Al-Munawarah, Saudi Arabia"],"prefix":"10.3390","volume":"15","author":[{"given":"Omar","family":"Almutairi","sequence":"first","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9050-2079","authenticated-orcid":false,"given":"Ihab M.","family":"Moussa","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1699-6263","authenticated-orcid":false,"given":"Eman","family":"Marzouk","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Applied Medical Sciences, Qassim University, P.O. Box 6666, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2294-9837","authenticated-orcid":false,"given":"Adil","family":"Abalkhail","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Applied Medical Sciences, Qassim University, P.O. Box 6666, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7726-9532","authenticated-orcid":false,"given":"Ayman","family":"Elbehiry","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Applied Medical Sciences, Qassim University, P.O. Box 6666, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (WHO) (2025, September 01). Estimating the Burden of Foodborne Diseases. Available online: https:\/\/www.who.int\/activities\/estimating-the-burden-of-foodborne-diseases."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4674235","DOI":"10.1155\/2020\/4674235","article-title":"Review on major food-borne zoonotic bacterial pathogens","volume":"2020","author":"Abebe","year":"2020","journal-title":"J. Trop. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4645","DOI":"10.2147\/IDR.S365269","article-title":"Review of Escherichia coli O157: H7 prevalence, pathogenicity, heavy metal and antimicrobial resistance, African perspective","volume":"15","author":"Gambushe","year":"2022","journal-title":"Infect. Drug Resist."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Elbehiry, A., Marzouk, E., Abalkhail, A., Edrees, H.M., Ellethy, A.T., Almuzaini, A.M., Ibrahem, M., Almujaidel, A., Alzaben, F., and Alqrni, A. (2025). Microbial Food Safety and Antimicrobial Resistance in Foods: A Dual Threat to Public Health. Microorganisms, 13.","DOI":"10.3390\/microorganisms13071592"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s40550-022-00092-7","article-title":"Fresh produce as a potential vehicle for transmission of Acinetobacter baumannii","volume":"9","author":"Ababneh","year":"2022","journal-title":"Int. J. Food Contam."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Farrow, J.M., Wells, G., and Pesci, E.C. (2018). Desiccation tolerance in Acinetobacter baumannii is mediated by the two-component response regulator BfmR. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0205638"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"113890","DOI":"10.1016\/j.ab.2020.113890","article-title":"Detection of Acinetobacter baumannii in fresh produce using modified magnetic nanoparticles and PCR","volume":"609","author":"Farouk","year":"2020","journal-title":"Anal. Biochem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"23886","DOI":"10.1038\/s41598-025-06410-1","article-title":"ESBL-producing Enterobacterales in food and clinical samples: Antimicrobial resistance organisms and genes in Chiang Mai, Thailand","volume":"15","author":"Dokuta","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s40249-023-01113-z","article-title":"Zoonotic sources and the spread of antimicrobial resistance from the perspective of low and middle-income countries","volume":"12","author":"Olaru","year":"2023","journal-title":"Infect. Dis. Poverty"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1038\/s41467-024-45111-7","article-title":"Global surveillance of antimicrobial resistance in food animals using priority drugs maps","volume":"15","author":"Zhao","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_11","first-page":"743","article-title":"Antimicrobial resistance: One health approach","volume":"15","year":"2022","journal-title":"Vet. World"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2015.04.016","article-title":"Ready-to-eat street-vended food as a potential vehicle of bacterial pathogens and antimicrobial resistance: An exploratory study in Porto region, Portugal","volume":"206","author":"Campos","year":"2015","journal-title":"Int. J. Food Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1002\/fsn3.1319","article-title":"Microbial load and antibiotic resistance in raw beef preparations from northwest Spain","volume":"8","author":"Capita","year":"2020","journal-title":"Food Sci. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bookari, K. (2023). What is the level of nutrition literacy of Saudi adolescents? A national wide exploratory cross-sectional study. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.1113910"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Mumena, W.A., Ateek, A.A., Alamri, R.K., Alobaid, S.A., Alshallali, S.H., Afifi, S.Y., Aljohani, G.A., and Kutbi, H.A. (2022). Fast-food consumption, dietary quality, and dietary intake of adolescents in Saudi Arabia. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph192215083"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Abalkhail, A. (2023). Frequency and antimicrobial resistance patterns of foodborne pathogens in ready-to-eat foods: An evolving public health challenge. Appl. Sci., 13.","DOI":"10.3390\/app132312846"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s13568-024-01710-7","article-title":"Proteome analysis, genetic characterization, and antibiotic resistance patterns of Klebsiella pneumoniae clinical isolates","volume":"14","author":"Marzouk","year":"2024","journal-title":"AMB Express"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"804484","DOI":"10.3389\/fmicb.2021.804484","article-title":"Combination of MALDI-TOF mass spectrometry and machine learning for rapid antimicrobial resistance screening: The case of Campylobacter spp.","volume":"12","author":"Feucherolles","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"113582","DOI":"10.1016\/j.ab.2020.113582","article-title":"Principal component analysis of MALDI-TOF MS of whole-cell foodborne pathogenic bacteria","volume":"592","author":"Yan","year":"2020","journal-title":"Anal. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Aladhadh, M. (2023). A review of modern methods for the detection of foodborne pathogens. Microorganisms, 11.","DOI":"10.3390\/microorganisms11051111"},{"key":"ref_21","first-page":"2825","article-title":"Microbiological evaluation of ready-to-eat foods by conventional methods and MALDI-TOF MS","volume":"72","year":"2021","journal-title":"J. Hell. Vet. Med. Soc."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Algammal, A.M., Enany, M.E., El-Tarabili, R.M., Ghobashy, M.O., and Helmy, Y.A. (2020). Prevalence, antimicrobial resistance profiles, virulence and enterotoxins-determinant genes of MRSA isolated from subclinical bovine mastitis in Egypt. Pathogens, 9.","DOI":"10.3390\/pathogens9050362"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Madani, A., Esfandiari, Z., Shoaei, P., and Ataei, B. (2022). Evaluation of virulence factors, antibiotic resistance, and biofilm formation of Escherichia coli isolated from milk and dairy products in Isfahan, Iran. Foods, 11.","DOI":"10.3390\/foods11070960"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"797","DOI":"10.4269\/ajtmh.20-1563","article-title":"The Hajj and COVID-19: How the pandemic shaped the world\u2019s largest religious gathering","volume":"104","author":"Hashim","year":"2021","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e48","DOI":"10.1016\/S0140-6736(20)30466-9","article-title":"COVID-19: Preparing for superspreader potential among Umrah pilgrims to Saudi Arabia","volume":"395","author":"Ebrahim","year":"2020","journal-title":"Lancet"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"297","DOI":"10.4014\/jmb.1310.10013","article-title":"Advances in rapid detection methods for foodborne pathogens","volume":"24","author":"Zhao","year":"2014","journal-title":"J Microbiol Biotechnol."},{"key":"ref_27","first-page":"1","article-title":"Evaluation of bacterial load and Multi-drug Antibacterial resistant Salmonella and Clostridium isolates from contaminated tools used in butcheries around Ibadan","volume":"7","author":"Fasanmi","year":"2023","journal-title":"Mathews J. Vet. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"100202","DOI":"10.1016\/j.jfp.2023.100202","article-title":"Food safety practices of food handlers in China and their correlation with self-reported foodborne illness","volume":"87","author":"Chen","year":"2024","journal-title":"J. Food Prot."},{"key":"ref_29","unstructured":"(2018). Microbiology of the Food Chain\u2014Horizontal Methods for Surface Sampling (Standard No. ISO 18593:2018). Available online: https:\/\/www.iso.org\/standard\/64950.html."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Havelaar, A.H., Kirk, M.D., Torgerson, P.R., Gibb, H.J., Hald, T., Lake, R.J., Praet, N., Bellinger, D.C., De Silva, N.R., and Gargouri, N. (2015). World Health Organization global estimates and regional comparisons of the burden of foodborne disease in 2010. PLoS Med., 12.","DOI":"10.1371\/journal.pmed.1001923"},{"key":"ref_31","unstructured":"WHO (2025, August 22). Estimates of the Global Burden of Foodborne Diseases: Foodborne Disease Burden Epidemiology Reference Group, 2007\u20132015. Geneva, Switzerland. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789241565165."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Da Silva, N., Taniwaki, M.H., Junqueira, V.C., Silveira, N., Okazaki, M.M., and Gomes, R.A.R. (2018). Microbiological Examination Methods of Food and Water: A Laboratory Manual, CRC Press.","DOI":"10.1201\/9781315165011"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Ayoub Moubareck, C., and Hammoudi Halat, D. (2020). Insights into Acinetobacter baumannii: A review of microbiological, virulence, and resistance traits in a threatening nosocomial pathogen. Antibiotics, 9.","DOI":"10.3390\/antibiotics9030119"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1128\/CMR.00058-07","article-title":"Acinetobacter baumannii: Emergence of a successful pathogen","volume":"21","author":"Peleg","year":"2008","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1834","DOI":"10.1128\/JCM.02654-12","article-title":"Identification of Gram-positive cocci by use of matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry: Comparison of different preparation methods and implementation of a practical algorithm for routine diagnostics","volume":"51","author":"Schulthess","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1007\/s00253-011-3783-4","article-title":"MALDI-TOF MS in microbiological diagnostics\u2014Identification of microorganisms and beyond (mini review)","volume":"93","author":"Wieser","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1128\/CMR.00072-12","article-title":"Matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry: A fundamental shift in the routine practice of clinical microbiology","volume":"26","author":"Clark","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_38","unstructured":"CLSI (2023). Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute. [33rd ed.]."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_40","unstructured":"EUCAST (2025, August 21). Breakpoint Tables for Interpretation of MICs and Zone Diameters, Version 13.0. Available online: https:\/\/www.eucast.org\/."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3350","DOI":"10.1128\/aem.62.9.3350-3354.1996","article-title":"Confirmational identification of Escherichia coli: A comparison of genotypic and phenotypic assays for glutamate decarboxylase and \u03b2-D-glucuronidase","volume":"62","author":"McDaniels","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1128\/jcm.30.7.1654-1660.1992","article-title":"Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene","volume":"30","author":"Brakstad","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0890-8508(92)90002-F","article-title":"Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella","volume":"6","author":"Rahn","year":"1992","journal-title":"Mol. Cell. Probes"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2974","DOI":"10.1128\/JCM.01021-06","article-title":"Identification of Acinetobacter baumannii by detection of the bla OXA-51-like carbapenemase gene intrinsic to this species","volume":"44","author":"Turton","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0378-1097(03)00306-9","article-title":"Simple and reliable multiplex PCR assay for detection of bla TEM, bla SHV and bla OXA\u20131 genes in Enterobacteriaceae","volume":"223","author":"Colom","year":"2003","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.ijantimicag.2006.01.004","article-title":"Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.","volume":"27","author":"Woodford","year":"2006","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3018","DOI":"10.1128\/AAC.43.12.3018","article-title":"Genetic characterization of antimicrobial resistance in Canadian isolates of Salmonella serovar Typhimurium DT104","volume":"43","author":"Ng","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3953","DOI":"10.1128\/AAC.00915-06","article-title":"Prevalence in the United States of aac (6\u2032)-Ib-cr encoding a ciprofloxacin-modifying enzyme","volume":"50","author":"Park","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1128\/AAC.47.4.1423-1426.2003","article-title":"Multiplex PCR for detection of aminoglycoside resistance genes in enterococci","volume":"47","author":"Vakulenko","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1093\/jac\/dkf164","article-title":"Susceptibility of Danish Escherichia coli strains isolated from urinary tract infections and bacteraemia, and distribution of sul genes conferring sulphonamide resistance","volume":"50","author":"Kerrn","year":"2002","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1128\/jcm.29.10.2240-2244.1991","article-title":"Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction","volume":"29","author":"Murakami","year":"1991","journal-title":"J. Clin. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1128\/AAC.44.2.231-238.2000","article-title":"Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis","volume":"44","author":"Martineau","year":"2000","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2562","DOI":"10.1128\/AAC.40.11.2562","article-title":"Detection of erythromycin-resistant determinants by PCR","volume":"40","author":"Sutcliffe","year":"1996","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1128\/AAC.48.1.348-351.2004","article-title":"Genetic and biochemical characterization of a chromosome-encoded carbapenem-hydrolyzing Ambler class D \u03b2-lactamase from Shewanella algae","volume":"48","author":"Poirel","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s12941-015-0103-3","article-title":"Characteristics of multidrug-resistant Acinetobacter baumannii strains isolated in Geneva during colonization or infection","volume":"14","author":"Cherkaoui","year":"2015","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Mekhloufi, O.A., Chieffi, D., Hammoudi, A., Bensefia, S.A., Fanelli, F., and Fusco, V. (2021). Prevalence, enterotoxigenic potential and antimicrobial resistance of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) isolated from Algerian ready to eat foods. Toxins, 13.","DOI":"10.3390\/toxins13120835"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"11786361221113916","DOI":"10.1177\/11786361221113916","article-title":"Bacteriological quality and public health risk of ready-to-eat foods in developing countries: Systematic review and meta analysis","volume":"15","author":"Mengistu","year":"2022","journal-title":"Microbiol. Insights"},{"key":"ref_58","first-page":"460","article-title":"Prevalence of methicillin-resistant Staphylococcus aureus in some ready-to-eat meat products","volume":"4","author":"Saad","year":"2019","journal-title":"Am. J. Biomed. Sci. Res."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Elsalkh, S.A., Zakaria, A.I., Abd-Elghany, S.M., Imre, K., Morar, A., and Sallam, K.I. (2025). Prevalence and Characterization of the Antimicrobial Resistance and Virulence Profiles of Staphylococcus aureus in Ready-to-Eat (Meat, Chicken, and Tuna) Pizzas in Mansoura City, Egypt. Antibiotics, 14.","DOI":"10.3390\/antibiotics14080817"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"159","DOI":"10.4236\/aim.2022.123013","article-title":"Monitoring of Escherichia coli, Salmonella spp. and Staphylococci in poultry meat-based fast food in Saudi Arabia","volume":"12","author":"Mohamed","year":"2022","journal-title":"Adv. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"48","DOI":"10.14715\/cmb\/2025.71.8.8","article-title":"Prevalence, characterization, and transmissible factors of foodborne pathogens in the Al-Qassim Region, Saudi Arabia","volume":"71","author":"Almujaidel","year":"2025","journal-title":"Cell. Mol. Biol."},{"key":"ref_62","first-page":"754","article-title":"Detection of Escherichia coli, Salmonella spp. and Staphylococcus aureus in ready-to-eat food in Al-Ahsa Province, Saudi Arabia","volume":"9","year":"2019","journal-title":"J. Nutr. Food Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1179\/joc.2009.21.5.514","article-title":"First Identification of bla OXA-51-like in Non-baumannii Acinetobacter spp.","volume":"21","author":"Lee","year":"2009","journal-title":"J. Chemother."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1186\/s13756-018-0405-2","article-title":"Genotyping and distribution of putative virulence factors and antibiotic resistance genes of Acinetobacter baumannii strains isolated from raw meat","volume":"7","author":"Tavakol","year":"2018","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1016\/j.sjbs.2020.11.052","article-title":"Acinetobacter baumannii as a community foodborne pathogen: Peptide mass fingerprinting analysis, genotypic of biofilm formation and phenotypic pattern of antimicrobial resistance","volume":"28","author":"Elbehiry","year":"2021","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"763","DOI":"10.4315\/0362-028X.JFP-16-355","article-title":"A meta-analysis of the global prevalence rates of Staphylococcus aureus and methicillin-resistant S. aureus contamination of different raw meat products","volume":"80","author":"Ou","year":"2017","journal-title":"J. Food Prot."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Xing, L., Cheng, M., Jiang, J., Li, T., Zhang, X., Tian, Y., and Liu, W. (2025). Methicillin-resistant Staphylococcus aureus contamination in meat and meat products: A systematic review and meta-analysis. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1636622"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Singhal, N., Kumar, M., Kanaujia, P.K., and Virdi, J.S. (2015). MALDI-TOF mass spectrometry: An emerging technology for microbial identification and diagnosis. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.00791"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Elbehiry, A., and Abalkhail, A. (2025). Spectral Precision: Recent Advances in Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry for Pathogen Detection and Resistance Profiling. Microorganisms, 13.","DOI":"10.3390\/microorganisms13071473"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"20251136","DOI":"10.1515\/biol-2025-1136","article-title":"Innovations in MALDI-TOF Mass Spectrometry: Bridging modern diagnostics and historical insights","volume":"20","author":"Andreadi","year":"2025","journal-title":"Open Life Sci."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Kluz, M.I., Waszkiewicz-Robak, B., and Ka\u010d\u00e1niov\u00e1, M. (2025). The Applications of MALDI-TOF MS in the Diagnosis of Microbiological Food Contamination. Appl. Sci., 15.","DOI":"10.3390\/app15147863"},{"key":"ref_72","first-page":"269","article-title":"Application of MALDI-TOF MS fingerprinting as a quick tool for identification and clustering of foodborne pathogens isolated from food products","volume":"40","author":"Elbehiry","year":"2017","journal-title":"New Microbiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"462","DOI":"10.5530\/ajbls.2021.10.61","article-title":"Isolation and characterization of Acinetobacter baumannii from chicken meat samples in north India","volume":"10","author":"Kumar","year":"2021","journal-title":"Asian J. Biol. Life Sci"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Khater, D.F., Lela, R.A., El-Diasty, M., Moustafa, S.A., and Wareth, G. (2021). Detection of harmful foodborne pathogens in food samples at the points of sale by MALDT-TOF MS in Egypt. BMC Res. Notes, 14.","DOI":"10.1186\/s13104-021-05533-8"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.cofs.2016.11.004","article-title":"MALDI-TOF MS identification and tracking of food spoilers and food-borne pathogens","volume":"10","author":"Brul","year":"2016","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Giraud-Gatineau, A., Texier, G., Fournier, P.-E., Raoult, D., and Chaudet, H. (2021). Using MALDI-TOF spectra in epidemiological surveillance for the detection of bacterial subgroups with a possible epidemic potential. BMC Infect. Dis., 21.","DOI":"10.1186\/s12879-021-06803-3"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Elbehiry, A., Abalkhail, A., Marzouk, E., Elmanssury, A.E., Almuzaini, A.M., Alfheeaid, H., Alshahrani, M.T., Huraysh, N., Ibrahem, M., and Alzaben, F. (2023). An overview of the public health challenges in diagnosing and controlling human foodborne pathogens. Vaccines, 11.","DOI":"10.3390\/vaccines11040725"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Rahi, P., Prakash, O., and Shouche, Y.S. (2016). Matrix-assisted laser desorption\/ionization time-of-flight mass-spectrometry (MALDI-TOF MS) based microbial identifications: Challenges and scopes for microbial ecologists. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.01359"},{"key":"ref_79","first-page":"1","article-title":"Benefits and limitations of MALDI-TOF mass spectrometry for the identification of microorganisms","volume":"2","author":"Rychert","year":"2019","journal-title":"J. Infect. Epidemiol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.jfda.2019.01.001","article-title":"Current status of MALDI-TOF mass spectrometry in clinical microbiology","volume":"27","author":"Hou","year":"2019","journal-title":"J. Food Drug Anal."},{"key":"ref_81","first-page":"392","article-title":"Mechanizmy oporno\u015bci Acinetobacter baumannii na zwi\u0105zki przeciwbakteryjne","volume":"54","author":"Laudy","year":"2015","journal-title":"Adv. Microbiol."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Wang, W., Baloch, Z., Jiang, T., Zhang, C., Peng, Z., Li, F., Fanning, S., Ma, A., and Xu, J. (2017). Enterotoxigenicity and antimicrobial resistance of Staphylococcus aureus isolated from retail food in China. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.02256"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Yang, X., Zhang, J., Yu, S., Wu, Q., Guo, W., Huang, J., and Cai, S. (2016). Prevalence of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in retail ready-to-eat foods in China. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00816"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"12","DOI":"10.21608\/bvmj.2019.15641.1063","article-title":"Prevalence of methicillin-resistant Staphylococcus aureus in some ready-to-eat meat products","volume":"37","author":"Saif","year":"2019","journal-title":"Benha Vet. Med. J."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s41182-021-00366-4","article-title":"A survey of prevalence and phenotypic and genotypic assessment of antibiotic resistance in Staphylococcus aureus bacteria isolated from ready-to-eat food samples collected from Tehran Province, Iran","volume":"49","author":"Mesbah","year":"2021","journal-title":"Trop. Med. Health"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Irrgang, A., Falgenhauer, L., Fischer, J., Ghosh, H., Guiral, E., Guerra, B., Schmoger, S., Imirzalioglu, C., Chakraborty, T., and Hammerl, J.A. (2017). CTX-M-15-producing E. coli isolates from food products in Germany are mainly associated with an IncF-type plasmid and belong to two predominant clonal E. coli lineages. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.02318"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"143","DOI":"10.2147\/IDR.S34941","article-title":"Prevalence of extended-spectrum beta-lactamase-producing bacteria in food","volume":"5","author":"Tham","year":"2012","journal-title":"Infect. Drug Resist."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Zhou, L., Ye, Q., Zhou, Q., Wang, J., Li, G., Xiang, J., Huang, J., Zhao, Y., Zheng, T., and Zuo, H. (2024). Antimicrobial resistance and genomic investigation of Salmonella isolated from retail foods in Guizhou, China. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1345045"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Li, J., Zhou, R., Xu, Q., Qu, S., Lin, H., Wang, Y., Li, P., and Zheng, X. (2023). Serotyping and antimicrobial resistance profiling of multidrug-resistant non-typhoidal Salmonella from farm animals in Hunan, China. Antibiotics, 12.","DOI":"10.3390\/antibiotics12071178"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"102684","DOI":"10.1016\/j.jksus.2023.102684","article-title":"MALDI-TOF MS-based identification and antibiotics profiling of Salmonella species isolated from retail chilled chicken in Saudi Arabia","volume":"35","author":"Alharbi","year":"2023","journal-title":"J. King Saud Univ. Sci."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1128\/CMR.00109-13","article-title":"Coagulase-negative staphylococci","volume":"27","author":"Becker","year":"2014","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1111\/j.1469-0691.2006.01456.x","article-title":"Carbapenem resistance in Acinetobacter baumannii: Mechanisms and epidemiology","volume":"12","author":"Poirel","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_93","first-page":"e000306","article-title":"Emergence, molecular mechanisms and global spread of carbapenem-resistant Acinetobacter baumannii","volume":"5","author":"Hamidian","year":"2019","journal-title":"Microb. Genom."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6976.1996.tb00251.x","article-title":"Tetracycline resistance determinants: Mechanisms of action, regulation of expression, genetic mobility, and distribution","volume":"19","author":"Roberts","year":"1996","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Van Hoek, A.H., Mevius, D., Guerra, B., Mullany, P., Roberts, A.P., and Aarts, H.J. (2011). Acquired antibiotic resistance genes: An overview. Front. Microbiol., 2.","DOI":"10.3389\/fmicb.2011.00203"},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Azizi, H., Davtalab Esmaeili, E., Naghili, B., Ghanbarzadeh Javid, S., Sarbazi, E., and Abbasi, F. (2024). Risk factors for diarrheal diseases among pilgrims during Arba\u2019een mass gathering: A case-control study. BMC Infect. Dis., 24.","DOI":"10.1186\/s12879-024-09962-1"},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Alnafisah, R., Alnasiri, F., Alzaharni, S., Alshikhi, I., and Alqahtani, A. (2023). Food safety practices during Hajj: On-site inspections of food-serving establishments. Trop. Med. Infect. Dis., 8.","DOI":"10.3390\/tropicalmed8100480"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Hendriksen, R.S., Bortolaia, V., Tate, H., Tyson, G.H., Aarestrup, F.M., and McDermott, P.F. (2019). Using genomics to track global antimicrobial resistance. Front. Public Health, 7.","DOI":"10.3389\/fpubh.2019.00242"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1038\/s41576-023-00649-y","article-title":"Genomic surveillance for antimicrobial resistance\u2014A One Health perspective","volume":"25","author":"Djordjevic","year":"2024","journal-title":"Nat. Rev. Genet."},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Bianconi, I., Aschbacher, R., and Pagani, E. (2023). Current uses and future perspectives of genomic technologies in clinical microbiology. Antibiotics, 12.","DOI":"10.3390\/antibiotics12111580"},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Elbehiry, A., Marzouk, E., Alzaben, F., Almuaither, A., Abead, B., Alamri, M., Almuzaini, A.M., and Abu-Okail, A. (2025). Emerging Technologies and Integrated Strategies for Microbial Detection and Control in Fresh Produce. Microorganisms, 13.","DOI":"10.3390\/microorganisms13071447"}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/1\/104\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:50:18Z","timestamp":1767628218000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/1\/104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,5]]},"references-count":101,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["biology15010104"],"URL":"https:\/\/doi.org\/10.3390\/biology15010104","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,5]]}}}