{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T10:48:49Z","timestamp":1790160529323,"version":"4.1.0"},"reference-count":49,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Foundation Abdelwaheb Ben Ayed (FABA), Tunisia","award":["FABA2025-MJ"],"award-info":[{"award-number":["FABA2025-MJ"]}]},{"name":"Tunisian Ministry of Higher Education and Scientific Research MES","award":["PEJC2025-D1P12"],"award-info":[{"award-number":["PEJC2025-D1P12"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Whole-genome duplication, or tetraploidization, occurs in cells, tissues, or entire organisms. In human cancers, tetraploidization promotes aneuploidy and genomic instability, accelerating tumor progression, metastasis, and drug resistance. These adaptations demand metabolic rewiring, including mitochondrial plasticity. Here, we investigate the relationship between mitochondrial quantity\/activity, including the mitochondrial transmembrane potential, the intracellular calcium, and the oxidative stress in diploid versus tetraploid cancer cells (colon, sarcoma, liver) and fungal and yeast models (C. albicans diploid\/tetraploid strains; S. cerevisiae haploid\/diploid\/tetraploid strains). We demonstrate that tetraploid cells, whether from human carcinomas or yeast, exhibit consistently enlarged cell size, elevated mitochondrial content, and heightened metabolic activity compared to diploids. Our findings underscore mitochondrial adaptation as a hallmark of tetraploidization, offering novel therapeutic targets for chromosomally unstable tumors.<\/jats:p>","DOI":"10.3390\/biology15020181","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T11:35:27Z","timestamp":1768822527000},"page":"181","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mitochondrial Adaptations Underlying Tetraploidization in Human Cancer, Fungal, and Yeast Models"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4905-1020","authenticated-orcid":false,"given":"Mohamed","family":"Jema\u00e0","sequence":"first","affiliation":[{"name":"Human Genetics Laboratory, Faculty of Medicine of Tunis, Tunis El Manar University, 5, Rue Hassouna Ben Ayed\u20131007 Bab Saadoun Tunis, Tunis 2092, Tunisia"},{"name":"Neurophysiology, Cellular Physiopathology and Valorisation of Biomolecules Laboratory, Faculty of Sciences of Tunis, Tunis El Manar University, Tunis 2092, Tunisia"},{"name":"Department of Biology, Faculty of Sciences of Tunis, Tunis El Manar University, Campus Universitaire El-Manar, 2092 El Manar, Tunis 2092, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ameni","family":"Bedoui","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biotechnology of Eukaryotes, LMBE, Centre of Biotechnology of Sfax, P.O. Box 1177, Sfax 3038, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5744-0328","authenticated-orcid":false,"given":"Nihel","family":"Ammous","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biotechnology of Eukaryotes, LMBE, Centre of Biotechnology of Sfax, P.O. Box 1177, Sfax 3038, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5009-9000","authenticated-orcid":false,"given":"Ali","family":"Gargouri","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biotechnology of Eukaryotes, LMBE, Centre of Biotechnology of Sfax, P.O. Box 1177, Sfax 3038, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0594-6343","authenticated-orcid":false,"given":"Mohamed","family":"Guerfali","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biotechnology of Eukaryotes, LMBE, Centre of Biotechnology of Sfax, P.O. Box 1177, Sfax 3038, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Schvarzstein, M., Alam, F., Toure, M., and Yanowitz, J.L. (2023). An Emerging Animal Model for Querying the Role of Whole Genome Duplication in Development, Evolution, and Disease. J. Dev. 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