{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:02:53Z","timestamp":1790290973130,"version":"4.1.0"},"reference-count":83,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T00:00:00Z","timestamp":1773532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004505","name":"University of Catania","doi-asserted-by":"crossref","award":["57722172151."],"award-info":[{"award-number":["57722172151."]}],"id":[{"id":"10.13039\/501100004505","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Colorectal cancer (CRC) is still a leading cause of cancer-related death worldwide, and often, conventional chemotherapeutics exhibit limited efficacy. The hydroalcoholic leaf extract of Cynara cardunculus subsp. cardunculus (wild artichoke) was investigated for its anticancer potential in CRC and effects on enteric pathogens. Nine phenolic compounds were identified by high-performance liquid chromatography with diode-array detection (HPLC-DAD), and spectrophotometric analyses were applied for total phenolic (TPC: 178.33 mg GAE\/g) and total flavonoid (TFC: 52.21 mg CE\/g) content quantification. The extract exhibited good antioxidant activity on DPPH (IC50: 21.35 \u03bcg\/mL), \u2212\u2022O2 (IC50: 1.56 \u03bcg\/mL), and H2O2 (IC50: 314.73 \u03bcg\/mL) and was found to inhibit the growth of pathogenic enteric bacteria, with Enterococcus faecalis and Staphylococcus aureus being the most sensitive. In CaCo-2 CRC cells, the extract induced a concentration-dependent cytotoxicity (IC50: 13.07 \u03bcg\/mL at 24 h) through increased production of reactive oxygen species (ROS), upregulation of Nrf2, and induction of apoptosis, as evidenced by elevated p53, Bax, cytochrome c, and caspase-3 levels. No necrosis, measured by lactate dehydrogenase (LDH) release, or toxicity to HFF-1 normal fibroblasts was observed at concentrations up to 50 \u03bcg\/mL. Additionally, CCE demonstrated synergistic effects with 5-FU (combination index &lt; 0.8). This evidence suggests that CCE exhibits selective antitumor activity and chemosensitizing properties, supporting its possible development as an adjunctive agent in CRC therapy.<\/jats:p>","DOI":"10.3390\/biology15060475","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:13:14Z","timestamp":1773659594000},"page":"475","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cynara cardunculus subsp. cardunculus (Wild Artichoke) Extract: Antimicrobial Activity and Cytotoxicity, Apoptosis Induction, and Chemosensitization in Colon Cancer Cells"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0521-3534","authenticated-orcid":false,"given":"Simone","family":"Bianchi","sequence":"first","affiliation":[{"name":"Department of Drug and Health Science, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy"},{"name":"Research Centre on Nutraceuticals and Health Products (CERNUT), University of Catania, Viale A. 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Doria 6, 95125 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6049-942X","authenticated-orcid":false,"given":"Barbara","family":"Tomasello","sequence":"additional","affiliation":[{"name":"Department of Drug and Health Science, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy"},{"name":"Research Centre on Nutraceuticals and Health Products (CERNUT), University of Catania, Viale A. 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Doria 6, 95125 Catania, Italy"},{"name":"Department of Agriculture, Food and Environment, University of Catania, Via Santa Sofia 100, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3186-6355","authenticated-orcid":false,"given":"Irina","family":"Naletova","sequence":"additional","affiliation":[{"name":"Institute of Crystallography, Research National Council, Via Paolo Gaifami, 18, 95126 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donata","family":"Condorelli","sequence":"additional","affiliation":[{"name":"Department of Drug and Health Science, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfonsina","family":"La Mantia","sequence":"additional","affiliation":[{"name":"Department of Drug and Health Science, University of Catania, Via Valdisavoia, 5, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7761-0662","authenticated-orcid":false,"given":"Ignazio","family":"Barbagallo","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences, University of Catania, Via Santa Sofia 97, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1243-5357","authenticated-orcid":false,"given":"Cinzia","family":"Randazzo","sequence":"additional","affiliation":[{"name":"Research Centre on Nutraceuticals and Health Products (CERNUT), University of Catania, Viale A. 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