{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:35:30Z","timestamp":1790354130901,"version":"4.1.0"},"reference-count":62,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T00:00:00Z","timestamp":1774137600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Interreg North-West Europe project BioBoost Pro","award":["NWE0400559\u2013BIOBOOST-PRO"],"award-info":[{"award-number":["NWE0400559\u2013BIOBOOST-PRO"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Aquaponic systems are among the sustainable approaches for combining fish farming and plant cultivation and have been proposed as potentially economically viable food production technologies. Their performance depends on the balanced environmental conditions shared by fish, nitrifying bacteria, and plants. This study assessed the performance of a trout-based (Oncorhynchus mykiss) decoupled aquaponic system for basil (Ocimum basilicum L.) growth and essential oil composition. Two cultivation strategies were compared over 60 days: a non-supplemented system relying exclusively on trout rearing water, and a system supplemented with mineral nutrients formulated according to the recommendations of the Hoagland nutrient solution. Basil grown without mineral supplementation maintained a healthy appearance and stable physiological status, with satisfactory growth, although it remained lower than in supplemented plants. The mineral profile of the plants showed similar nitrate and phosphorus concentrations between non-supplemented and supplemented plants, with nitrate levels of 5.40 \u00b1 0.29 mg g\u22121 and 5.52 \u00b1 0.29 mg g\u22121, respectively, and phosphorus levels of 5.46 \u00b1 0.23 mg g\u22121 and 6.14 \u00b1 0.91 mg g\u22121, respectively. In contrast, potassium concentration was lower in non-supplemented plants (36.89 \u00b1 3.31 mg g\u22121) compared to supplemented plants (55.56 \u00b1 7.16 mg g\u22121). Essential oil yield expressed per cultivated surface area remained comparable between systems, reaching 2.96 and 3.09 mL m\u22122 in the supplemented and non-supplemented systems, respectively. GC\u2013MS analysis revealed that linalool (\u224824%) was the predominant compound in both systems. Notably, estragole content was higher in non-supplemented plants (21.35 \u00b1 1.46%) compared to supplemented plants (5.24 \u00b1 0.68%). Overall, trout-based aquaponic systems not only support satisfactory basil growth but also enhance the production of essential oils with desirable aromatic characteristics, representing a sustainable and efficient strategy for aromatic plant cultivation.<\/jats:p>","DOI":"10.3390\/biology15060511","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T13:07:06Z","timestamp":1774271226000},"page":"511","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Agronomic Performance, Mineral Composition, and Biochemical Characteristics of Basil (Ocimum basilicum L.) Grown in Trout (Oncorhynchus mykiss) Aquaponic Systems"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1992-011X","authenticated-orcid":false,"given":"Mohammed","family":"Elakrouch","sequence":"first","affiliation":[{"name":"Laboratory of Integrated and Urban Plant Pathology (LIUPP), Gembloux Agro-Bio Tech, University of Liege, Passage des D\u00e9port\u00e9s 2, 5030 Gembloux, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7791-368X","authenticated-orcid":false,"given":"Marouane","family":"Mohaddab","sequence":"additional","affiliation":[{"name":"Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, University of Li\u00e8ge, Passage des D\u00e9port\u00e9s 2, 5030 Gembloux, Belgium"},{"name":"Laboratory of Agrifood and Health, Faculty of Sciences and Techniques, Hassan First University of Settat, BP 577, Settat 26000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Elmoussaoui","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Universit\u00e9 Libre de Bruxelles (ULB), 1050 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Libault","sequence":"additional","affiliation":[{"name":"Laboratory of Integrated and Urban Plant Pathology (LIUPP), Gembloux Agro-Bio Tech, University of Liege, Passage des D\u00e9port\u00e9s 2, 5030 Gembloux, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Rachid","sequence":"additional","affiliation":[{"name":"Laboratory of Innovative Technology, University of Picardie Jules Verne, 33 Rue Saint LEU, 80000 Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1011-4047","authenticated-orcid":false,"given":"M. Haissam","family":"Jijakli","sequence":"additional","affiliation":[{"name":"Laboratory of Integrated and Urban Plant Pathology (LIUPP), Gembloux Agro-Bio Tech, University of Liege, Passage des D\u00e9port\u00e9s 2, 5030 Gembloux, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,22]]},"reference":[{"key":"ref_1","first-page":"63","article-title":"Soilless agriculture: A new and advanced method for agriculture development: An introduction","volume":"3","year":"2017","journal-title":"Adv. Res. Technol. Open Access J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2265106","DOI":"10.1080\/23311932.2023.2265106","article-title":"Soilless culture technology to transform vegetable farming, reduce land pressure and degradation in drylands","volume":"9","author":"Gebreegziher","year":"2023","journal-title":"Cogent Food Agric."},{"key":"ref_3","first-page":"2663","article-title":"Soilless farming: An innovative sustainable approach in agriculture","volume":"11","author":"Mir","year":"2022","journal-title":"Pharma Innov. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"608","DOI":"10.9734\/ijecc\/2024\/v14i23976","article-title":"Role of hydroponics in improving water-use efficiency and food security","volume":"14","author":"Naresh","year":"2024","journal-title":"Int. J. Environ. Clim. Change"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Aslanidou, M., Elvanidi, A., Mourantian, A., Levizou, E., Mente, E., and Katsoulas, N. (2023). Nutrient use efficiency in coupled and decoupled aquaponic systems. Horticulturae, 9.","DOI":"10.3390\/horticulturae9101077"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e42339","DOI":"10.1016\/j.heliyon.2025.e42339","article-title":"Comparing yield, nutrient uptake and water use efficiency of Nasturtium officinale cultivated in aquaponic, hydroponic, and soil systems","volume":"11","author":"Alizaeh","year":"2025","journal-title":"Heliyon"},{"key":"ref_7","first-page":"33","article-title":"Aquaponics: A sustainable technology for aquaculture and agriculture food security","volume":"3","author":"Mohamad","year":"2025","journal-title":"Planet. Sustain."},{"key":"ref_8","first-page":"1","article-title":"Nutrient film technique (NFT) hydroponic monitoring system","volume":"1","author":"Helmy","year":"2016","journal-title":"J. Adv. Inf. Commun. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Aires, L.M.I., Ispolnov, K., Luz, T.R., Pala, H., and Vieira, J.S. (2023). Optimization of an indoor DWC hydroponic lettuce production system to generate a low N and P content wastewater. Processes, 11.","DOI":"10.3390\/pr11020365"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"102595","DOI":"10.1016\/j.aqrep.2024.102595","article-title":"Aquaponics model can provide better productivity: Microorganisms affect the growth and health of tilapia","volume":"40","author":"Fan","year":"2025","journal-title":"Aquac. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.jssas.2021.04.001","article-title":"Production performance and financial feasibility analysis of farming catfish (Clarias gariepinus) utilizing water exchange system, aquaponics, and biofloc technology","volume":"20","author":"Diatin","year":"2021","journal-title":"J. Saudi Soc. Agric. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2642434","DOI":"10.1155\/2024\/2642434","article-title":"A literature review of tilapia\/lettuce aquaponics: Production status, varieties, and research gaps","volume":"2024","author":"Hussain","year":"2024","journal-title":"Aquac. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"02006","DOI":"10.1051\/e3sconf\/20184702006","article-title":"Water quality, survival, and growth of red tilapia, Oreochromis niloticus, cultured in aquaponic systems","volume":"47","author":"Setiadi","year":"2018","journal-title":"E3S Web Conf."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.jksus.2018.02.001","article-title":"Effects of different growth media on water quality and plant yield in a catfish-pumpkin aquaponics system","volume":"32","author":"Oladimeji","year":"2020","journal-title":"J. King Saud Univ. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.aquaeng.2005.09.004","article-title":"Nitrification kinetics of biofilm as affected by water quality factors","volume":"34","author":"Chen","year":"2006","journal-title":"Aquac. Eng."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/j.1745-4557.2006.00053.x","article-title":"Effects of variety and growth season on the organoleptic and nutritional quality of hydroponically grown tomato","volume":"29","author":"Anza","year":"2006","journal-title":"J. Food Qual."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6879","DOI":"10.3390\/ijerph120606879","article-title":"Comparison of land, water, and energy requirements of lettuce grown using hydroponic vs. conventional agricultural methods","volume":"12","author":"Barbosa","year":"2015","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Fruscella, L., Kotzen, B., Paradelo Perez, M., and Milliken, S. (2025). Investigating the effects of fish effluents as organic fertilisers on basil (Ocimum basilicum). Appl. Sci., 15.","DOI":"10.3390\/app15031563"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Turnsek, M., Joly, A., Thorarinsdottir, R., and Junge, R. (2020). Challenges of commercial aquaponics in Europe: Beyond the hype. Water, 12.","DOI":"10.3390\/w12010306"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6","DOI":"10.21273\/HORTTECH.21.1.6","article-title":"Opportunities and challenges to sustainability in aquaponic systems","volume":"21","author":"Tyson","year":"2011","journal-title":"HortTechnology"},{"key":"ref_21","unstructured":"Coll\u00e8ge des Producteurs (2022). \u00c9tude du March\u00e9 Belge du Poisson Issu de L\u2019aquaculture, Coll\u00e8ge des Producteurs. Available online: https:\/\/collegedesproducteurs.be\/wp-content\/uploads\/2023\/01\/Etude-du-marche-belge-du-poisson-issu-de-laquaculture.pdf."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"coaf025","DOI":"10.1093\/conphys\/coaf025","article-title":"Effects of elevated temperature on gene expression, energy metabolism, and physiology in brown trout, Salmo trutta","volume":"13","author":"Hampuwo","year":"2025","journal-title":"Conserv. Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.procbio.2007.11.005","article-title":"Comparison study of the effects of temperature and free ammonia concentration on nitrification and nitrite accumulation","volume":"43","author":"Kim","year":"2008","journal-title":"Process Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"925","DOI":"10.21273\/HORTSCI16690-22","article-title":"Effects of elevated water temperature on growth of basil using nutrient film technique","volume":"57","author":"Hendrickson","year":"2022","journal-title":"HortScience"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Yavuzcan Yildiz, H., Robaina, L., Pirhonen, J., Mente, E., Dom\u00ednguez, D., and Parisi, G. (2017). Fish welfare in aquaponic systems: Its relation to water quality with an emphasis on feed and faeces\u2014A review. Water, 9.","DOI":"10.3390\/w9010013"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Chrysargyris, A., Louka, S., Petropoulos, S.A., and Tzortzakis, N. (2023). Soilless cultivation of Portulaca oleracea using medicinal and aromatic plant residues for partial peat replacement. Horticulturae, 9.","DOI":"10.3390\/horticulturae9040474"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Maggini, R., Tzortzakis, N., and Currey, C.J. (2022). Editorial: Soilless culture for vegetative biomass production and specialized metabolites: Medicinal, aromatic, and edible plants. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.887487"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1002\/ffj.1513","article-title":"Chemical characterization of basil (Ocimum spp.) based on volatile oils","volume":"21","author":"Vieira","year":"2006","journal-title":"Flavour Fragr. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1080\/10942912.2020.1828456","article-title":"Chemical components and pharmacological benefits of basil (Ocimum basilicum): A review","volume":"23","author":"Shahrajabian","year":"2020","journal-title":"Int. J. Food Prop."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"100183","DOI":"10.1109\/ACCESS.2025.3571730","article-title":"Optimizing nutrient formulations through artificial intelligence model to reduce excessive fertigation in lettuce grown in hydroponic systems","volume":"13","author":"Mohmed","year":"2025","journal-title":"IEEE Access"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/BF00018060","article-title":"Rapid determination of free proline for water-stress studies","volume":"39","author":"Bates","year":"1973","journal-title":"Plant Soil"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/978-1-4020-3218-9_12","article-title":"Analysis of the chlorophyll a fluorescence transient","volume":"Volume 19","author":"Papageorgiou","year":"2004","journal-title":"Chlorophyll a Fluorescence"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"146202","DOI":"10.1016\/j.jclepro.2025.146202","article-title":"The impact of two aquaponic management strategies (unfertilized and fertilized aquaponics) on the quality and growth of green butterhead lettuce (Lactuca sativa var. Salanova Aquino, Rijk Zwaan)","volume":"521","author":"Benmansour","year":"2025","journal-title":"J. Clean. Prod."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1080\/00103627509366547","article-title":"Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid","volume":"6","author":"Cataldo","year":"1975","journal-title":"Commun. Soil Sci. Plant Anal."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.scienta.2018.11.016","article-title":"Bioactive compounds and antioxidant activity of Pimpinella anisum L. accessions at different ripening stages","volume":"246","author":"Kaab","year":"2019","journal-title":"Sci. Hortic."},{"key":"ref_36","first-page":"12","article-title":"Extraction of green absolute from thyme using ultrasound and sunflower oil","volume":"3","author":"Mnayer","year":"2017","journal-title":"Resour.-Eff. Technol."},{"key":"ref_37","first-page":"209","article-title":"Role of nitrogen for plant growth and development: A review","volume":"10","author":"Leghari","year":"2016","journal-title":"Adv. Environ. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3828","DOI":"10.1038\/s41598-025-33937-0","article-title":"Foliar application of potassium and iron enhances biomass and essential oil production of basil cultivated in aquaponics and hydroponics","volume":"16","author":"Roosta","year":"2026","journal-title":"Sci. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.aoas.2016.10.001","article-title":"Growth, yield, plant quality and nutrition of basil (Ocimum basilicum L.) under soilless agricultural systems","volume":"61","author":"Saha","year":"2016","journal-title":"Ann. Agric. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Rodgers, D., Won, E., Timmons, M.B., and Mattson, N. (2022). Complementary nutrients in decoupled aquaponics enhance basil performance. Horticulturae, 8.","DOI":"10.3390\/horticulturae8020111"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1080\/01904167.2014.890220","article-title":"Comparison of the vegetative growth, eco-physiological characteristics and mineral nutrient content of basil plants in different irrigation ratios of hydroponic:aquaponic solutions","volume":"37","author":"Roosta","year":"2014","journal-title":"J. Plant Nutr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1111\/pce.12157","article-title":"Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue?","volume":"37","author":"Sreenivasulu","year":"2014","journal-title":"Plant Cell Environ."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Al-Gaadi, K.A., Tola, E., Madugundu, R., Zeyada, A.M., Alameen, A.A., Edrris, M.K., Edrees, H.F., and Mahjoop, O. (2024). Response of leaf photosynthesis, chlorophyll content, and yield of hydroponic tomatoes to different water salinity levels. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0293098"},{"key":"ref_44","unstructured":"Nishanth, D. (2023). Standardizing Sustainable Aquaponic Production of Leafy Greens and Fish: A Comparison with Conventional Systems in the United Arab Emirates. [Master\u2019s Thesis, United Arab Emirates University]."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Signorini, L., Modarelli, G.C., Di Pierro, P., Langellotti, A.L., Cirillo, C., De Pascale, S., and Masi, P. (2025). Effects of Seedling Substrate and Hydroponic versus Aquaponic Nutrient Solution on Growth, Nutrient Uptake, and Eco-Physiological Response of Lemon Basil (Ocimum \u00d7 citriodorum). Plants, 14.","DOI":"10.3390\/plants14131929"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"109412","DOI":"10.1016\/j.agwat.2025.109412","article-title":"Evaluating hydroponics and aquaponics: Comparative insights into sustainability and strawberry quality","volume":"312","author":"Korbee","year":"2025","journal-title":"Agric. Water Manag."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Khare, N., Khare, P., and Singh, S. (2025). Molecular and Physiological Concepts: Role of Macronutrients in the Growth and Development of Cultivated Plants. Improvement of Agricultural Crops, CRC Press.","DOI":"10.1201\/9781032630366-11"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Yang, T., and Kim, H.-J. (2020). Characterizing nutrient composition and concentration in tomato-, basil-, and lettuce-based aquaponic and hydroponic systems. Water, 12.","DOI":"10.3390\/w12051259"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.copbio.2021.11.010","article-title":"Diversity: Current and prospective secondary metabolites for nutrition and medicine","volume":"74","author":"Sreenivasulu","year":"2022","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.foodres.2012.03.015","article-title":"Volatile composition of sweet basil essential oil (Ocimum basilicum L.) as affected by drying method","volume":"48","author":"Lech","year":"2012","journal-title":"Food Res. Int."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Tangpao, T., Charoimek, N., Teerakitchotikan, P., Leksawasdi, N., Jantanasakulwong, K., Rachtanapun, P., Seesuriyachan, P., Phimolsiripol, Y., Chaiyaso, T., and Ruksiriwanich, W. (2022). Volatile organic compounds from basil essential oils: Plant taxonomy, biological activities, and their applications in tropical fruit productions. Horticulturae, 8.","DOI":"10.3390\/horticulturae8020144"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1080\/10412905.2021.2022019","article-title":"Biological fertilizers and superabsorbent polymer change biomass and volatile oil composition of Ocimum ciliatum Hornem","volume":"34","author":"Moghaddam","year":"2022","journal-title":"J. Essent. Oil Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"363","DOI":"10.5423\/PPJ.NT.01.2016.0006","article-title":"Antibacterial activity of cinnamaldehyde and estragole extracted from plant essential oils against Pseudomonas syringae pv. actinidiae causing bacterial canker disease in kiwifruit","volume":"32","author":"Song","year":"2016","journal-title":"Plant Pathol. J."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Abdou, A., Maaghloud, F.E., Rammali, S., Benchama, Z., Kamal, F.Z., Abdelaziz, E.A., Elmakssoudi, A., and Dakir, M. (2025). Design, Synthesis, and Antibacterial Evaluation of Novel Thiosemicarbazone Derivatives from Estragole. Nat. Prod. Res., 1\u20139.","DOI":"10.1080\/14786419.2025.2608710"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"104951","DOI":"10.1016\/j.bse.2024.104951","article-title":"Effects of domestication on essential oil yield, chemical profiling, and antifungal activities of bitter fennel (Foeniculum vulgare Mill.)","volume":"120","author":"Ok","year":"2025","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e01081","DOI":"10.1002\/cbdv.202501081","article-title":"Chemical composition and insecticidal activity of Ocimum basilicum and Ocimum \u00d7 africanum essential oil","volume":"22","author":"Gu","year":"2025","journal-title":"Chem. Biodivers."},{"key":"ref_57","first-page":"15","article-title":"Estragole and Anethole as Potential Hazardous Compounds in Products: Extraction, Analysis and Pharmacological Effects","volume":"9","author":"Dief","year":"2025","journal-title":"Rec. Pharm. Biomed. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"537","DOI":"10.33899\/ijvs.2022.135092.2445","article-title":"Estragole: A review of its pharmacology, effect on animal health and performance, toxicology, and market regulatory issues","volume":"37","author":"Mahendra","year":"2023","journal-title":"Iraqi J. Vet. Sci."},{"key":"ref_59","unstructured":"Scientific Committee on Food (2026, March 06). Opinion of the Scientific Committee on Food on Estragole (1-Allyl-4-methoxybenzene), Available online: https:\/\/ec.europa.eu\/food\/system\/files\/2020-12\/sci-com_scf_out104_en.pdf."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"120291","DOI":"10.1016\/j.indcrop.2024.120291","article-title":"Optimizing nitrogen, phosphorus, and potassium requirements to improve Origanum dubium Boiss. growth, nutrient and water use efficiency, essential oil yield and composition","volume":"224","author":"Chrysargyris","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_61","first-page":"321","article-title":"Influence of nitrogen nutrition on growth and accumulation of rosmarinic acid in sweet basil (Ocimum basilicum L.) grown in hydroponic culture","volume":"7","author":"Kiferle","year":"2013","journal-title":"Aust. J. Crop Sci."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Re\u017eek Jambrak, A. (2022). Efficient production of functional and bioactive compounds and foods for use in food, pharma, cosmetic and other industries. Nonthermal Processing in Agri-Food-Bio Sciences: Sustainability and Future Goals, Springer.","DOI":"10.1007\/978-3-030-92415-7"}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/6\/511\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T13:35:28Z","timestamp":1774272928000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/6\/511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,22]]},"references-count":62,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["biology15060511"],"URL":"https:\/\/doi.org\/10.3390\/biology15060511","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,22]]}}}