{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T10:46:44Z","timestamp":1776854804257,"version":"3.51.2"},"reference-count":68,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondazione CRT (Collirio biologico da Concentrato Piastrinico da Sangue Cordonale: una nuo-va possibile terapia per la secchezza oculare","award":["(Protocollo: 2019.AI1619.U1833)"],"award-info":[{"award-number":["(Protocollo: 2019.AI1619.U1833)"]}]},{"name":"NextGeneration EU and Compagnia di San Paolo","award":["NextGeneration EU and Compagnia di San Paolo"],"award-info":[{"award-number":["NextGeneration EU and Compagnia di San Paolo"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Umbilical cord-derived Platelet-Rich Plasma (ucPRP) promotes corneal regeneration, yet the underlying cellular mechanisms remain incompletely understood. This study evaluated the effects of ucPRP on corneal epithelial cell physiology and wound healing dynamics. In scratch assays, ucPRP treatment significantly accelerated wound closure by enhancing cell migration and proliferation. Confocal microscopy using Fluo-3 demonstrated that ucPRP induces transient increases in intracellular calcium (Ca2+), suggesting the activation of upstream signaling events. Furthermore, treated cells exhibited increased membrane permeability to water and hydrogen peroxide (H2O2), which correlated with upregulation of Aquaporin-1 (AQP1). Since AQP1 facilitates cell motility, its upregulation links fluid transport mechanisms to the observed regenerative phenotype. These findings suggest that ucPRP drives corneal re-epithelialization by modulating Ca2+ signaling and increasing AQP1 expression, supporting its therapeutic utility in ocular surface repair.<\/jats:p>","DOI":"10.3390\/biology15080637","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T16:30:36Z","timestamp":1776443436000},"page":"637","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Umbilical Cord PRP Accelerates Corneal Wound Healing via AQP1 Upregulation and Calcium Signaling"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2891-1810","authenticated-orcid":false,"given":"Simona","family":"Martinotti","sequence":"first","affiliation":[{"name":"DiSIT\u2014Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Viale Teresa Michel 11, 15121 Alessandria, Italy"},{"name":"Laboratorio Integrato di Ricerca Preclinica, Azienda Universitaria Ospedaliera \u201cSS Antonio e Biagio e Cesare Arrigo\u201d, Via Venezia, 16, 15121 Alessandria, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giorgia","family":"Pellavio","sequence":"additional","affiliation":[{"name":"Human Physiology Unit, Department of Molecular Medicine, University of Pavia, 27100 Pavia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0225-5763","authenticated-orcid":false,"given":"Gregorio","family":"Bonsignore","sequence":"additional","affiliation":[{"name":"DiSIT\u2014Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Viale Teresa Michel 11, 15121 Alessandria, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2923-5389","authenticated-orcid":false,"given":"Valeria","family":"Balbo","sequence":"additional","affiliation":[{"name":"Transfusion Medicine and Regeneration Medicine, Azienda Universitaria Ospedaliera \u201cSS Antonio e Biagio e Cesare Arrigo\u201d, Via Venezia, 16, 15121 Alessandria, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Mazzucco","sequence":"additional","affiliation":[{"name":"Transfusion Medicine and Regeneration Medicine, Azienda Universitaria Ospedaliera \u201cSS Antonio e Biagio e Cesare Arrigo\u201d, Via Venezia, 16, 15121 Alessandria, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2951-5983","authenticated-orcid":false,"given":"Umberto","family":"Laforenza","sequence":"additional","affiliation":[{"name":"Human Physiology Unit, Department of Molecular Medicine, University of Pavia, 27100 Pavia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7600-8215","authenticated-orcid":false,"given":"Elia","family":"Ranzato","sequence":"additional","affiliation":[{"name":"DiSIT\u2014Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Viale Teresa Michel 11, 15121 Alessandria, Italy"},{"name":"Laboratorio Integrato di Ricerca Preclinica, Azienda Universitaria Ospedaliera \u201cSS Antonio e Biagio e Cesare Arrigo\u201d, Via Venezia, 16, 15121 Alessandria, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/bs.pmbts.2015.04.001","article-title":"Overview of the Cornea: Structure, Function, and Development","volume":"134","author":"Eghrari","year":"2015","journal-title":"Prog. Mol. Biol. Transl. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Wilson, S.E. (2020). Corneal wound healing. Exp. Eye Res., 197.","DOI":"10.1016\/j.exer.2020.108089"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Kumar, A., Yun, H., Funderburgh, M.L., and Du, Y. (2022). Regenerative therapy for the Cornea. Prog. Retin. Eye Res., 87.","DOI":"10.1016\/j.preteyeres.2021.101011"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.preteyeres.2015.07.002","article-title":"Progress in corneal wound healing","volume":"49","author":"Ljubimov","year":"2015","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Mohan, R.R., Kempuraj, D., D\u2019Souza, S., and Ghosh, A. (2022). Corneal stromal repair and regeneration. Prog. Retin. Eye Res., 91.","DOI":"10.1016\/j.preteyeres.2022.101090"},{"key":"ref_6","first-page":"282","article-title":"Limitations and advances in new treatments and future perspectives of corneal blindness","volume":"84","author":"Adriano","year":"2021","journal-title":"Arq. Bras. Oftalmol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mobaraki, M., Abbasi, R., Omidian Vandchali, S., Ghaffari, M., Moztarzadeh, F., and Mozafari, M. (2019). Corneal Repair and Regeneration: Current Concepts and Future Directions. Front. Bioeng. Biotechnol., 7.","DOI":"10.3389\/fbioe.2019.00135"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"496","DOI":"10.4103\/IJO.IJO_482_25","article-title":"Biological topicals in ocular surface disorders","volume":"73","author":"Nair","year":"2025","journal-title":"Indian J. Ophthalmol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1080\/17474086.2021.1860002","article-title":"Evidence-based indications of platelet-rich plasma therapy","volume":"14","author":"Gupta","year":"2021","journal-title":"Expert. Rev. Hematol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1007\/s12178-018-9527-7","article-title":"Current Clinical Recommendations for Use of Platelet-Rich Plasma","volume":"11","author":"Le","year":"2018","journal-title":"Curr. Rev. Musculoskelet. Med."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Li, Y., Wang, X., Li, D., Li, S., and Shen, C. (2024). Efficacy and safety of allogeneic platelet-rich plasma in chronic wound treatment: A meta-analysis of randomized controlled trials. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-75090-0"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.reth.2024.07.004","article-title":"Adverse events related to platelet-rich plasma therapy and future issues to be resolved","volume":"26","author":"Arita","year":"2024","journal-title":"Regen. Ther."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5308","DOI":"10.1016\/j.biomaterials.2012.04.007","article-title":"Adult and umbilical cord blood-derived platelet-rich plasma for mesenchymal stem cell proliferation, chemotaxis, and cryo-preservation","volume":"33","author":"Murphy","year":"2012","journal-title":"Biomaterials"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Eguren, A., de Miguel-G\u00f3mez, L., Franc\u00e9s-Herrero, E., G\u00f3mez-\u00c1lvarez, M., Faus, A., G\u00f3mez-Cerd\u00e1, M., Moret-Tatay, I., D\u00edaz, A., Pellicer, A., and Cervell\u00f3, I. (2023). Human umbilical cord platelet-rich plasma to treat endometrial pathologies: Methodology, composition and pre-clinical models. Hum. Reprod. Open, 2023.","DOI":"10.1093\/hropen\/hoac053"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Tang, Y., Li, Y., Chen, H., Huang, Y., Huang, C., and Wei, W. (2024). Application of cord blood-derived platelet-rich plasma in the treatment of diseases. J. Int. Med. Res., 52.","DOI":"10.1177\/03000605241263729"},{"key":"ref_16","first-page":"189","article-title":"Safety and efficacy of eye drops from umbilical cord blood platelet lysate to treat resistant corneal ulcer","volume":"11","author":"Dawood","year":"2022","journal-title":"Med. Hypothesis Discov. Innov. Ophthalmol."},{"key":"ref_17","first-page":"347","article-title":"Clinical evaluation of allogeneic eye drops from cord blood platelet lysate","volume":"19","author":"Samarkanova","year":"2021","journal-title":"Blood Transfus."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Gagliano, C., Foti, R., Zeppieri, M., Maniaci, A., Lavalle, S., Tancredi, G., Gagliano, G., Avitabile, A., and Cannizzaro, L. (2024). Umbilical Cord Blood Platelet Lysate Eyedrops for the Treatment of Severe Ocular Surface Disorders in Graft vs. Host Disease Patients: Clinical Study. Life, 14.","DOI":"10.3390\/life14101268"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"\u00d6zsan, M., Saygili D\u00fczova, \u00dc., and D\u00f6nmez, N. (2024). Neuroprotective role of curcumin on the hippocampus against the oxidative stress and inflammation of streptozotocin-induced diabetes in rats. Metab. Brain Dis., 40.","DOI":"10.1007\/s11011-024-01438-0"},{"key":"ref_20","first-page":"3279","article-title":"The Effect of Curcumin on the Antioxidant System in Diabetic Rats","volume":"72","year":"2021","journal-title":"J. Hell. Vet. Med. Soc."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4415","DOI":"10.3390\/cimb44100303","article-title":"Evaluation of the Regenerative Potential of Platelet-Lysate and Platelet-Poor Plasma Derived from the Cord Blood Units in Corneal Wound Healing Applications: An In Vitro Comparative Study on Corneal Epithelial Cells","volume":"44","author":"Mallis","year":"2022","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_22","first-page":"7","article-title":"A prospective single-center study of the efficacy and safety of using cord blood lysate-derived platelet concentrate eye drops for the treatment of severe ocular surface diseases","volume":"119","author":"Mazzucco","year":"2024","journal-title":"Vox Sang."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Tian, J., Li, X.J., Ma, Y., Mai, Z., Yang, Y., Luo, M., Xu, W., Chen, K., Chen, X., and Tang, J. (2023). Correlation of bioactive components of platelet rich plasma derived from human female adult peripheral blood and umbilical cord blood with age. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-45747-3"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1007\/s00424-007-0357-5","article-title":"Aquaporins and cell migration","volume":"456","author":"Papadopoulos","year":"2008","journal-title":"Pflug. Arch."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.exer.2009.03.002","article-title":"Aquaporin-1-facilitated keratocyte migration in cell culture and in vivo corneal wound healing models","volume":"89","author":"Verkman","year":"2009","journal-title":"Exp. Eye Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Karimi, N., and Ahmadi, V. (2024). Aquaporin Channels in Skin Physiology and Aging Pathophysiology: Investigating Their Role in Skin Function and the Hallmarks of Aging. Biology, 13.","DOI":"10.3390\/biology13110862"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Pellavio, G., Senise, G., Vicenzo, C.P., and Laforenza, U. (2025). Sigma1 Receptor Modulates Plasma Membrane and Mitochondrial Peroxiporins. Cells, 14.","DOI":"10.3390\/cells14141082"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Giallongo, S., Bellia, F., Russo, A., Fallico, M., Polosa, R., Castellino, N., Longo, A., Emma, R., Partsinevelos, K., and Caruso, M. (2025). Comparative Evaluation of Cigarette Smoke and a Heated Tobacco Product on Corneal Oxidative Stress in an Air\/Liquid Interface Model. Investig. Ophthalmol. Vis. Sci., 66.","DOI":"10.1167\/iovs.66.4.4"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/7651_2019_259","article-title":"Scratch Wound Healing Assay","volume":"2109","author":"Martinotti","year":"2020","journal-title":"Methods Mol. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"302","DOI":"10.2174\/0113892010328741240828093859","article-title":"Correlation between Honey Parameters and Wound Healing Properties: The Case of Piedmont (Italy) Samples","volume":"26","author":"Martinotti","year":"2025","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.ymeth.2008.09.025","article-title":"Chemical calcium indicators","volume":"46","author":"Paredes","year":"2008","journal-title":"Methods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2667","DOI":"10.1111\/j.1582-4934.2012.01584.x","article-title":"Epigallocatechin-3-gallate induces mesothelioma cell death via H2O2-dependent T-type Ca2+ channel opening","volume":"16","author":"Ranzato","year":"2012","journal-title":"J. Cell Mol. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3440","DOI":"10.1016\/S0021-9258(19)83641-4","article-title":"A new generation of Ca2+ indicators with greatly improved fluorescence properties","volume":"260","author":"Grynkiewicz","year":"1985","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.cmet.2020.02.003","article-title":"Ultrasensitive Genetically Encoded Indicator for Hydrogen Peroxide Identifies Roles for the Oxidant in Cell Migration and Mitochondrial Function","volume":"31","author":"Pak","year":"2020","journal-title":"Cell Metab."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Pellavio, G., Rossino, G., Gastaldi, G., Rossi, D., Linciano, P., Collina, S., and Laforenza, U. (2021). Sigma-1 Receptor Agonists Acting on Aquaporin-Mediated H2O2 Permeability: New Tools for Counteracting Oxidative Stress. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22189790"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"120","DOI":"10.2174\/1389201015666140604121407","article-title":"Platelet-rich plasma induces mixed osteogenic\/osteoclastogenic phenotype in osteosarcoma SaOS-2 cells: Role of TGF-beta","volume":"15","author":"Martinotti","year":"2014","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2030","DOI":"10.1111\/j.1582-4934.2008.00467.x","article-title":"Platelet Lysate Promotes in Vitro Wound Scratch Closure of Human Dermal Fibroblasts: Different Roles of Cell Calcium, P38, ERK, and PI3K\/AKT","volume":"13","author":"Ranzato","year":"2008","journal-title":"J. Cell. Mol. Med."},{"key":"ref_39","first-page":"537","article-title":"Platelet lysate stimulates wound repair of HaCaT keratinocytes","volume":"159","author":"Ranzato","year":"2008","journal-title":"Br. J. Dermatol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/j.cellbi.2009.06.017","article-title":"Scratch wound closure of C2C12 mouse myoblasts is enhanced by human platelet lysate","volume":"33","author":"Ranzato","year":"2009","journal-title":"Cell Biol. Int."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1002\/jcb.22591","article-title":"Role of ERK1\/2 in platelet lysate-driven endothelial cell repair","volume":"110","author":"Ranzato","year":"2010","journal-title":"J. Cell. Biochem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1111\/j.1600-0625.2010.01173.x","article-title":"Platelet lysate modulates MMP-2 and MMP-9 expression, matrix deposition and cell-to-matrix adhesion in keratinocytes and fibroblasts","volume":"20","author":"Ranzato","year":"2011","journal-title":"Exp. Dermatol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/sj.bjp.0703534","article-title":"The p38 MAP kinase inhibitor SB203580 enhances nuclear factor-kappa B transcriptional activity by a non-specific effect upon the ERK pathway","volume":"131","author":"Birkenkamp","year":"2000","journal-title":"Br. J. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1124\/mol.53.3.438","article-title":"PD98059 is an equipotent antagonist of the aryl hydrocarbon receptor and inhibitor of mitogen-activated protein kinase kinase","volume":"53","author":"Reiners","year":"1998","journal-title":"Mol. Pharmacol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"You, Y., Niu, Y., Zhang, J., Huang, S., Ding, P., Sun, F., and Wang, X. (2022). U0126: Not only a MAPK kinase inhibitor. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.927083"},{"key":"ref_46","first-page":"483","article-title":"The epidermal growth factor tyrosine kinase inhibitor AG1478 and erlotinib reverse ABCG2-mediated drug resistance","volume":"21","author":"Shi","year":"2009","journal-title":"Oncol. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Martinotti, S., Patrone, M., Balbo, V., Mazzucco, L., and Ranzato, E. (2020). Endothelial response boosted by platelet lysate: The involvement of calcium toolkit. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21030808"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1002\/term.195","article-title":"Long-term effect of platelet lysate on primary fibroblasts highlighted with a proteomic approach","volume":"3","author":"Cipriani","year":"2009","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.apsb.2016.11.001","article-title":"Targeting calcium signaling in cancer therapy","volume":"7","author":"Cui","year":"2017","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0167-4889(96)00113-9","article-title":"Effects of U73122 and U73343 on human platelet calcium signalling and protein tyrosine phosphorylation","volume":"1355","author":"Heemskerk","year":"1997","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1200\/JCO.1989.7.4.499","article-title":"Suramin: An anticancer drug with a unique mechanism of action","volume":"7","author":"Stein","year":"1989","journal-title":"J. Clin. Oncol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1089\/jop.2016.0009","article-title":"Purinergic Signaling in Corneal Wound Healing: A Tale of 2 Receptors","volume":"32","author":"Minns","year":"2016","journal-title":"J. Ocul. Pharmacol. Ther."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"C972","DOI":"10.1152\/ajpcell.00110.2013","article-title":"Hypoxia-induced changes in Ca2+ mobilization and protein phosphorylation implicated in impaired wound healing","volume":"306","author":"Lee","year":"2014","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"21989","DOI":"10.1074\/jbc.M302650200","article-title":"p38 and ERK1\/2 coordinate cellular migration and proliferation in epithelial wound healing: Evidence of cross-talk activation between MAP kinase cascades","volume":"278","author":"Sharma","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1016\/j.jid.2019.02.004","article-title":"SOX2 Epidermal Overexpression Promotes Cutaneous Wound Healing via Activation of EGFR\/MEK\/ERK Signaling Mediated by EGFR Ligands","volume":"139","author":"Uchiyama","year":"2019","journal-title":"J. Investig. Dermatol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Herrera, A., Morcuende, S., Talaver\u00f3n, R., Ben\u00edtez-Temi\u00f1o, B., Pastor, A.M., and Matarredona, E.R. (2021). Purinergic Receptor Blockade with Suramin Increases Survival of Postnatal Neural Progenitor Cells In Vitro. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22020713"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0014-2999(92)90004-N","article-title":"Suramin: A selective inhibitor of purinergic neurotransmission in the rat isolated vas deferens","volume":"220","author":"Mallard","year":"1992","journal-title":"Eur. J. Pharmacol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Vladulescu, D., Scurtu, L.G., Simionescu, A.A., Scurtu, F., Popescu, M.I., and Simionescu, O. (2023). Platelet-Rich Plasma (PRP) in Dermatology: Cellular and Molecular Mechanisms of Action. Biomedicines, 12.","DOI":"10.3390\/biomedicines12010007"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1111\/j.1476-5381.2010.00771.x","article-title":"The phospholipase C inhibitor U-73122 inhibits Ca2+ release from the intracellular sarcoplasmic reticulum Ca2+ store by inhibiting Ca2+ pumps in smooth muscle","volume":"160","author":"Macmillan","year":"2010","journal-title":"Br. J. Pharmacol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"F13","DOI":"10.1152\/ajprenal.2000.278.1.F13","article-title":"Structure and function of aquaporin water channels","volume":"278","author":"Verkman","year":"2000","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1002\/jcp.25078","article-title":"Aquaporin-1 plays important role in proliferation by affecting cell cycle progression","volume":"231","year":"2016","journal-title":"J. Cell. Physiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1038\/nature03460","article-title":"Impairment of angiogenesis and cell migration by targeted aquaporin-1 gene disruption","volume":"434","author":"Saadoun","year":"2005","journal-title":"Nature"},{"key":"ref_63","first-page":"51","article-title":"Aquaporin-1 expression is decreased in human and mouse corneal endothelial dysfunction","volume":"10","author":"Macnamara","year":"2004","journal-title":"Mol. Vis."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1016\/j.bbrc.2020.02.031","article-title":"Lens aquaporins function as peroxiporins to facilitate membrane transport of hydrogen peroxide","volume":"524","author":"Varadaraj","year":"2020","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1016\/j.freeradbiomed.2011.06.001","article-title":"Mechanism of hydrogen peroxide-induced keratinocyte migration in a scratch-wound model","volume":"51","author":"Loo","year":"2011","journal-title":"Free Radic. Biol. Med."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Loo, A.E., Wong, Y.T., Ho, R., Wasser, M., Du, T., Ng, W.T., and Halliwell, B. (2012). Effects of hydrogen peroxide on wound healing in mice in relation to oxidative damage. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0049215"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1159\/000475501","article-title":"Hydrogen Peroxide: A Potential Wound Therapeutic Target?","volume":"26","author":"Zhu","year":"2017","journal-title":"Med. Princ. Pract."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1111\/j.1524-475X.2008.00410.x","article-title":"Growth factors and cytokines in wound healing","volume":"16","author":"Barrientos","year":"2008","journal-title":"Wound Repair Regen."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/8\/637\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T09:57:15Z","timestamp":1776851835000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/8\/637"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,17]]},"references-count":68,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["biology15080637"],"URL":"https:\/\/doi.org\/10.3390\/biology15080637","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,17]]}}}