{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T14:08:54Z","timestamp":1779458934852,"version":"3.53.1"},"reference-count":55,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Zhejiang Province Agricultural Major Technology Cooperative Extension Program","award":["2023ZDXT15"],"award-info":[{"award-number":["2023ZDXT15"]}]},{"name":"Research and Demonstration on High-Efficiency Artificial Insemination Breeding Technology for Geese","award":["H20230639"],"award-info":[{"award-number":["H20230639"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Goose blood has anticancer properties and was recorded in ancient China, but the specific molecular mechanisms underlying this effect still require further exploration. In this study, SW1990 cells were treated with goose serum or plasma, and transcriptome analysis was performed to explore the function of goose blood on cancer cells. Metabolomic profiling was also performed on goose serum, goose plasma, chicken serum, and chicken plasma to identify the bioactive substances responsible for the anticancer effect. The study examined the effects of goose plasma and serum on SW1990 cells and compared the metabolites between goose and chicken blood. Wound scratch, CCK-8, and Annexin V-PI assays showed that goose plasma and serum inhibited SW1990 cell proliferation at 24 and 48 h. Both treatments reduced cell viability, with serum inducing early and late apoptosis and plasma inducing late apoptosis. RNA sequencing (RNA-seq) identified 2259 (1418 upregulated, 841 downregulated) and 2731 (1844 upregulated, 887 downregulated) differentially expressed genes (DEGs) in the plasma and serum groups versus the negative control (NC), respectively, and 689 DEGs between the plasma and serum groups. Gene Ontology (GO) and KEGG pathway analyses revealed that the DEGs were enriched in processes such as lipid metabolism, JAK-STAT, and IL-17 pathways. Untargeted liquid chromatography\u2013tandem mass spectrometry (LC-MS\/MS) analysis identified distinct metabolites in goose and chicken blood, with unique metabolites and differential ones between groups. In SW1990 cells, four metabolite subclusters matched the plasma and serum effects. In summary, goose blood can suppress cancer cells by regulating gene expression to affect the key signaling pathways involved in cancer cell apoptosis and autophagy. Certain metabolites present at high concentrations in goose blood, such as cucurbitacin D and Oleoyl-L-carnitine, may also contribute to the inhibition of cancer cell proliferation and migration. These findings suggest that goose blood holds broad application prospects as a future auxiliary drug for cancer treatment, and this study provides a theoretical basis for the further application of goose products.<\/jats:p>","DOI":"10.3390\/biology15100788","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T13:12:08Z","timestamp":1779455528000},"page":"788","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomic and Metabolomic Profiling Reveals the Antiproliferative Mechanism of Goose Serum and Plasma in SW1990 Cells"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7174-7756","authenticated-orcid":false,"given":"Xiaolong","family":"Zhou","sequence":"first","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, 666 Wusu Road, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihan","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, 666 Wusu Road, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4327-8884","authenticated-orcid":false,"given":"Han","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, 666 Wusu Road, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangchen","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, 666 Wusu Road, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songbai","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, 666 Wusu Road, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8789-7858","authenticated-orcid":false,"given":"Ayong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, 666 Wusu Road, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2129884","DOI":"10.1080\/08941939.2022.2129884","article-title":"Pancreatic Cancer: A Review of Current Treatment and Novel Therapies","volume":"36","author":"Kolbeinsson","year":"2023","journal-title":"J. Investig. Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1533033820962117","DOI":"10.1177\/1533033820962117","article-title":"Pancreatic Cancer: A Review of Risk Factors, Diagnosis, and Treatment","volume":"19","author":"Zhao","year":"2020","journal-title":"Technol. Cancer Res. Treat."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3179","DOI":"10.1158\/0008-5472.CAN-19-2731","article-title":"Pancreatic Adenocarcinoma: Unconventional Approaches for an Unconventional Disease","volume":"80","author":"Gromisch","year":"2020","journal-title":"Cancer Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4846","DOI":"10.3748\/wjg.v24.i43.4846","article-title":"Pancreatic cancer: A review of clinical diagnosis, epidemiology, treatment and outcomes","volume":"24","author":"McGuigan","year":"2018","journal-title":"World J. Gastroenterol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Fanijavadi, S., Thomassen, M., and Jensen, L.H. (2025). Targeting Triple NK Cell Suppression Mechanisms: A Comprehensive Review of Biomarkers in Pancreatic Cancer Therapy. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26020515"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1647\/2012-072","article-title":"Hematologic Parameters and Hemoparasites of Nonmigratory Canada Geese (Branta canadensis) from Greensboro, North Carolina, USA","volume":"28","author":"Rutledge","year":"2014","journal-title":"J. Avian Med. Surg."},{"key":"ref_7","first-page":"e124822","article-title":"Effect of white lion goose serum on gastric cancer cells","volume":"44","author":"Li","year":"2024","journal-title":"Food Sci. Technol."},{"key":"ref_8","first-page":"3065","article-title":"Establishment and characterization of the gemcitabine-resistant human pancreatic cancer cell line SW1990\/gemcitabine","volume":"18","author":"Yu","year":"2019","journal-title":"Oncol. Lett."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"975","DOI":"10.3892\/or.2013.2888","article-title":"Oridonin induces apoptosis in SW1990 pancreatic cancer cells via p53-and caspase-dependent induction of p38 MAPK","volume":"31","author":"Bu","year":"2014","journal-title":"Oncol. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1093\/bioinformatics\/btp612","article-title":"DEGseq: An R package for identifying differentially expressed genes from RNA-seq data","volume":"26","author":"Wang","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e12","DOI":"10.1002\/imt2.12","article-title":"Majorbio Cloud: A one-stop, comprehensive bioinformatic platform for multiomics analyses","volume":"1","author":"Ren","year":"2022","journal-title":"iMeta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e217","DOI":"10.1002\/imt2.217","article-title":"Majorbio Cloud 2024: Update single-cell and multiomics workflows","volume":"3","author":"Han","year":"2024","journal-title":"iMeta"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Zhang, Y.K., Xue, J.J., Chen, Y., Huang, X.F., Liu, Z.L., Zhong, H., Xie, Q., Luo, Y., Wang, Q.G., and Wang, C. (2024). Modulation of Performance, Plasma Constituents, Small Intestinal Morphology, and Cecum Microbiota in Growing Geese by Dietary Citric Acid Supplementation. Animals, 14.","DOI":"10.3390\/ani14050660"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.5713\/ajas.19.0842","article-title":"Comparison of overfed Xupu and Landes geese in performance, fatty acid composition, enzymes and gene expression related to lipid metabolism","volume":"33","author":"Liu","year":"2020","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Nies, J.F., and Panzer, U. (2020). IL-17C\/IL-17RE: Emergence of a Unique Axis in TH17 Biology. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.00341"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1038\/cmi.2015.105","article-title":"The roles and functional mechanisms of interleukin-17 family cytokines in mucosal immunity","volume":"13","author":"Song","year":"2016","journal-title":"Cell. Mol. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1089\/jir.2012.0053","article-title":"Toll-Like Receptor 5 Engagement Induces Interleukin-17C Expression in Intestinal Epithelial Cells","volume":"32","author":"Im","year":"2012","journal-title":"J. Interferon Cytokine Res."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhang, X.R., Li, B.J., Lan, T., Chiari, C., Ye, X.Y., Wang, K.P., and Chen, J. (2025). The role of interleukin-17 in inflammation-related cancers. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1479505"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/1756-9966-32-3","article-title":"High expression of IL-17 and IL-17RE associate with poor prognosis of hepatocellular carcinoma","volume":"32","author":"Liao","year":"2013","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Pires, B.R.B., Binato, R., Ferreira, G.M., Corr\u00eaa, S., Du Rocher, B., Bulzico, D., Crocamo, S., dos Santos, E.C., Lima, L.G., and Abdelhay, E. (2021). Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222212144"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.3892\/or.2020.7803","article-title":"Exploring the role of glucose-6-phosphate dehydrogenase in cancer","volume":"44","author":"Li","year":"2020","journal-title":"Oncol. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zeng, T., Li, B., Shu, X., Pang, J.H., Wang, H.P., Cai, X.H., Liao, Y.Y., Xiao, X.L., Chong, Y.T., and Gong, J. (2023). Pan-cancer analysis reveals that G6PD is a prognostic biomarker and therapeutic target for a variety of cancers. Front. Oncol., 13.","DOI":"10.3389\/fonc.2023.1183474"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5857","DOI":"10.1038\/s41467-024-50157-8","article-title":"Glucose-6-phosphate dehydrogenase maintains redox homeostasis and biosynthesis in LKB1-deficient KRAS-driven lung cancer","volume":"15","author":"Lan","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.freeradbiomed.2025.06.002","article-title":"Glucose-6-phosphate dehydrogenase (G6PD) shields pancreatic cancer from autophagy-dependent ferroptosis by suppressing redox imbalance induced AMPK\/mTOR signaling","volume":"237","author":"Deepak","year":"2025","journal-title":"Free Radic. Biol. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"S96","DOI":"10.1016\/j.jid.2024.06.563","article-title":"Blockade of glucose-6-phosphate dehydrogenase induces immunogenic cell death and accelerates immunotherapy","volume":"144","author":"Nakamura","year":"2024","journal-title":"J. Investig. Dermatol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Song, J.Q., Sun, H.R., Zhang, S., and Shan, C.L. (2022). The Multiple Roles of Glucose-6-Phosphate Dehydrogenase in Tumorigenesis and Cancer Chemoresistance. Life, 12.","DOI":"10.3390\/life12020271"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"116935","DOI":"10.1016\/j.biopha.2024.116935","article-title":"Glucose-6-phosphate dehydrogenase and transketolase: Key factors in breast cancer progression and therapy","volume":"176","author":"Zhen","year":"2024","journal-title":"Biomed. Pharmacother."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Li, X.M., Rasul, A., Sharif, F., and Hassan, M. (2024). PIAS family in cancer: From basic mechanisms to clinical applications. Front. Oncol., 14.","DOI":"10.3389\/fonc.2024.1376633"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.clinre.2010.12.002","article-title":"PIAS3 expression in human gastric carcinoma and its adjacent non-tumor tissues","volume":"35","author":"Liu","year":"2011","journal-title":"Clin. Res. Hepatol. Gastroenterol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1158\/0008-5472.CAN-17-1671","article-title":"STAT3\/PIAS3 Levels Serve as \u201cEarly Signature\u201d Genes in the Development of High-Grade Serous Carcinoma from the Fallopian Tube","volume":"78","author":"Saini","year":"2018","journal-title":"Cancer Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"287345","DOI":"10.1155\/2015\/287345","article-title":"RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress","volume":"2015","author":"Cho","year":"2015","journal-title":"Mediat. Inflamm."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3202","DOI":"10.1158\/0008-5472.CAN-11-0054","article-title":"Ribonucleotide reductase small subunit M2B prognoses better survival in colorectal cancer","volume":"71","author":"Liu","year":"2011","journal-title":"Cancer Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1038\/srep00822","article-title":"RRM2B suppresses activation of the oxidative stress pathway and is up-regulated by p53 during senescence","volume":"2","author":"Kuo","year":"2012","journal-title":"Sci. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e00046-18","DOI":"10.1128\/MCB.00046-18","article-title":"The RASSF6 Tumor Suppressor Protein Regulates Apoptosis and Cell Cycle Progression via Retinoblastoma Protein","volume":"38","author":"Hossain","year":"2018","journal-title":"Mol. Cell. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"ra59","DOI":"10.1126\/scisignal.2000300","article-title":"Hippo pathway-dependent and -independent roles of RASSF6","volume":"2","author":"Ikeda","year":"2009","journal-title":"Sci. Signal."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"19813","DOI":"10.18632\/oncotarget.7852","article-title":"Decreased level of RASSF6 in sporadic colorectal cancer and its anti-tumor effects both in vitro and in vivo","volume":"7","author":"Chen","year":"2016","journal-title":"Oncotarget"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9189","DOI":"10.2147\/OTT.S217041","article-title":"RASSF6 Is Downregulated in Human Bladder Cancers and Regulates Doxorubicin Sensitivity And Mitochondrial Membrane Potential Via the Hippo Signaling Pathway","volume":"12","author":"Tan","year":"2019","journal-title":"OncoTargets Ther."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2340","DOI":"10.1016\/j.bbrc.2018.06.159","article-title":"Downregulation of RASSF6 promotes breast cancer growth and chemoresistance through regulation of Hippo signaling","volume":"503","author":"He","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Smith, S., Lopez, S., Kim, A., Kasteri, J., Olumuyide, E., Punu, K., de la Parra, C., and Sauane, M. (2023). Interleukin 24: Signal Transduction Pathways. Cancers, 15.","DOI":"10.3390\/cancers15133365"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e180962","DOI":"10.1172\/jci.insight.180962","article-title":"CD4+ T helper 2 cell-macrophage crosstalk induces IL-24-mediated breast cancer suppression","volume":"10","author":"Wang","year":"2025","journal-title":"JCI Insight"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/978-1-4471-6458-6_6","article-title":"MDA-7\/IL-24: Multifunctional cancer killing cytokine","volume":"818","author":"Menezes","year":"2014","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1111\/j.1476-5381.2012.02213.x","article-title":"Oleoyl-L-carnitine inhibits glycine transport by GlyT2","volume":"168","author":"Carland","year":"2013","journal-title":"Br. J. Pharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Donisi, I., Balestrieri, A., Del Vecchio, V., Bifulco, G., Balestrieri, M.L., Campanile, G., and D\u2019Onofrio, N. (2025). l-Carnitine and Acetyl-l-Carnitine Induce Metabolism Alteration and Mitophagy-Related Cell Death in Colorectal Cancer Cells. Nutrients, 17.","DOI":"10.3390\/nu17061010"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Huang, H., Liu, N., Guo, H., Liao, S., Li, X., Yang, C., Liu, S., Song, W., Liu, C., and Guan, L. (2012). L-carnitine is an endogenous HDAC inhibitor selectively inhibiting cancer cell growth in vivo and in vitro. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0049062"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"5815","DOI":"10.18632\/oncotarget.6808","article-title":"Metabolomic profile in pancreatic cancer patients: A consensus-based approach to identify highly discriminating metabolites","volume":"7","author":"Mazza","year":"2016","journal-title":"Oncotarget"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1016\/j.apsb.2025.02.026","article-title":"Obesity-driven oleoylcarnitine accumulation in tumor microenvironment promotes breast cancer metastasis-like phenotype","volume":"15","author":"Chen","year":"2025","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"9328344","DOI":"10.1155\/2023\/9328344","article-title":"Clinical Significance of Carnitine in the Treatment of Cancer: From Traffic to the Regulation","volume":"2023","author":"Farahzadi","year":"2023","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Console, L., Scalise, M., Mazza, T., Pochini, L., Galluccio, M., Giangregorio, N., Tonazzi, A., and Indiveri, C. (2020). Carnitine Traffic in Cells. Link with Cancer. Front. Cell Dev. Biol., 8.","DOI":"10.3389\/fcell.2020.583850"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s11010-015-2509-9","article-title":"Cucurbitacin D induces cell cycle arrest and apoptosis by inhibiting STAT3 and NF-\u03baB signaling in doxorubicin-resistant human breast carcinoma (MCF7\/ADR) cells","volume":"409","author":"Ku","year":"2015","journal-title":"Mol. Cell. Biochem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/ajco.70009","article-title":"Purified Cucurbitacin D Leads to Alterations of Apoptotic and Autophagic Genes Expression in MDA-MB-468 and MCF-7 Human Breast Cancer Cells","volume":"22","author":"Zeinali","year":"2025","journal-title":"Asia-Pac. J. Clin. Oncol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"36594","DOI":"10.1038\/srep36594","article-title":"Cucurbitacin D exhibits potent anti-cancer activity in cervical cancer","volume":"6","author":"Sikander","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1007\/s12672-025-02056-7","article-title":"Uncovering the anti-cancer mechanism of cucurbitacin D against colorectal cancer through network pharmacology and molecular docking","volume":"16","author":"Lim","year":"2025","journal-title":"Discov. Oncol."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/10\/788\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T13:50:24Z","timestamp":1779457824000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/10\/788"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,15]]},"references-count":55,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["biology15100788"],"URL":"https:\/\/doi.org\/10.3390\/biology15100788","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,15]]}}}