{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T05:02:42Z","timestamp":1779685362798,"version":"3.53.1"},"reference-count":32,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T00:00:00Z","timestamp":1778889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"crossref","award":["2026JJ50050"],"award-info":[{"award-number":["2026JJ50050"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science and Technology Innovation Program of Hunan Province","award":["2025RC3190"],"award-info":[{"award-number":["2025RC3190"]}]},{"name":"National Innovation Training Program for College Students","award":["202510555071"],"award-info":[{"award-number":["202510555071"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Helicobacter pylori (H. pylori) infection is closely associated with the development of chronic gastritis, peptic ulcers, and gastric cancer, highlighting the importance of rapid and accurate detection for disease prevention and clinical management. In this study, a one-pot LAMP-CRISPR\/Cas12b assay targeting the CagA gene was developed for H. pylori detection. First, the LAMP system was optimized by systematically screening key reaction components. Subsequently, a one-step LAMP-CRISPR\/Cas12b detection platform was established through optimization of the ratio between the LAMP premix and CRISPR buffer, reaction temperature, Cas12b concentration, and ssDNA reporter concentration. Under optimal conditions, the assay achieved a detection limit of 3.14 \u00d7 101 copies\/\u00b5L, representing a tenfold improvement in sensitivity compared with conventional LAMP and PCR assays (3.14 \u00d7 102 copies\/\u00b5L). In addition, the entire detection process could be completed within 1 h. Validation using 17 culture-positive and 17 culture-negative samples demonstrated complete concordance with culture-based results, with no false-positive or false-negative detections observed. These findings indicate that the proposed platform possesses high sensitivity, excellent specificity, rapid turnaround, and operational simplicity, demonstrating strong potential for point-of-care testing and applications in resource-limited settings.<\/jats:p>","DOI":"10.3390\/biology15100797","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T02:32:28Z","timestamp":1779676348000},"page":"797","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["One-Pot LAMP-Coupled CRISPR\/Cas12b Assay Enables Sensitive Detection of Helicobacter pylori"],"prefix":"10.3390","volume":"15","author":[{"given":"Ziyan","family":"Tang","sequence":"first","affiliation":[{"name":"MOE Key Lab of Rare Pediatric Diseases, Hengyang Medical College, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentao","family":"Bai","sequence":"additional","affiliation":[{"name":"MOE Key Lab of Rare Pediatric Diseases, Hengyang Medical College, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuting","family":"Yan","sequence":"additional","affiliation":[{"name":"MOE Key Lab of Rare Pediatric Diseases, Hengyang Medical College, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaoming","family":"Luo","sequence":"additional","affiliation":[{"name":"MOE Key Lab of Rare Pediatric Diseases, Hengyang Medical College, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanheng","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Electrical Engineering, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuojun","family":"Bai","sequence":"additional","affiliation":[{"name":"Health Management Center, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Zhuzhou 412007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1246-3390","authenticated-orcid":false,"given":"Zhu","family":"Chen","sequence":"additional","affiliation":[{"name":"MOE Key Lab of Rare Pediatric Diseases, Hengyang Medical College, University of South China, Hengyang 421001, China"},{"name":"School of Electrical Engineering, University of South China, Hengyang 421001, China"},{"name":"Institute for Future Sciences, University of South China, Changsha 410008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1053\/j.gastro.2017.04.022","article-title":"Global Prevalence of Helicobacter pylori Infection: Systematic Review and Meta-Analysis","volume":"153","author":"Hooi","year":"2017","journal-title":"Gastroenterology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/5584_2019_363","article-title":"Malignant Helicobacter pylori-Associated Diseases: Gastric Cancer and MALT Lymphoma","volume":"1149","author":"Hatakeyama","year":"2019","journal-title":"Adv. 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