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Methods: This review discusses three synergistic mechanisms\u2014\u201ccascade degradation\u201d, \u201csymbiotic protection\u201d, and \u201cfunctional complementarity\u201d\u2014along with construction strategies including co-immobilization technology, engineered biofilms, and engineered bacteria modified via synthetic biology. Result: Synergistic platforms have achieved significant progress in treating various types of pharmaceutical pollutants, including antibiotics, anti-inflammatories and hormones, antiviral drugs and pesticides. Conclusions: The synergistic integration of enzymes and microorganisms achieves the unification of efficient catalysis and deep mineralization, opening up a new pathway for the remediation of pharmaceutical pollution. It also transforms theoretically existing concepts into operable treatment technologies.<\/jats:p>","DOI":"10.3390\/biology15100804","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T02:32:28Z","timestamp":1779676348000},"page":"804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic Integration of Enzyme and Microbial Platforms for Sustainable Management of Pharmaceutical Pollutants: Towards a Greener Pharmaceutical Lifecycle"],"prefix":"10.3390","volume":"15","author":[{"given":"Zhongshan","family":"Sun","sequence":"first","affiliation":[{"name":"School of Pharmacy & School of Biological and Food Engineering, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peitao","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy & School of Biological and Food Engineering, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyang","family":"Ge","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Jiangnan University, Wuxi 214126, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiguo","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Jiangnan University, Wuxi 214126, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1712-1346","authenticated-orcid":false,"given":"Huanmin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy & School of Biological and Food Engineering, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100076","DOI":"10.1016\/j.nexus.2022.100076","article-title":"Pharmaceutical wastewater as Emerging Contaminants (EC): Treatment technologies, impact on environment and human health","volume":"6","author":"Samal","year":"2022","journal-title":"Energy Nexus"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"32111","DOI":"10.1007\/s11356-021-14178-w","article-title":"Pharmaceutical effluent: A critical link in the interconnected ecosystem promoting antimicrobial resistance","volume":"28","author":"Kotwani","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s13756-020-0697-x","article-title":"Antimicrobial use and resistance in food-producing animals and the environment: An African perspective","volume":"9","author":"Kimera","year":"2020","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Tegegne, A.A., Mekasha, Y.T., Ayu, A.A., Hasen, G., and Suleman, S. (2024). A review on emerging pharmaceutical residues in Ethiopia: Occurrence, ecotoxicological aspects, and regulatory concerns. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1499487"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"167348","DOI":"10.1016\/j.scitotenv.2023.167348","article-title":"Spatial trends and ecotoxic risk assessment of selected pharmaceuticals in sediments from Lake Victoria, Uganda, East Africa","volume":"906","author":"Nantaba","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.scitotenv.2015.08.139","article-title":"Occurrence of selected antibiotics and antiretroviral drugs in Nairobi River Basin, Kenya","volume":"539","author":"Ngumba","year":"2016","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"101969","DOI":"10.1016\/j.toxrep.2025.101969","article-title":"Pollution by antimicrobials and antibiotic resistance genes in East Africa: Occurrence, sources, and potential environmental implications","volume":"14","author":"Hossein","year":"2025","journal-title":"Toxicol. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Horvat, O., and Kova\u010devi\u0107, Z. (2025). Human and Veterinary Medicine Collaboration: Synergistic Approach to Address Antimicrobial Resistance Through the Lens of Planetary Health. Antibiotics, 14.","DOI":"10.3390\/antibiotics14010038"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3435","DOI":"10.1073\/pnas.1222743110","article-title":"Diverse and abundant antibiotic resistance genes in Chinese swine farms","volume":"110","author":"Zhu","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7356","DOI":"10.1021\/acs.est.5b01012","article-title":"Antibiotic resistome and its association with bacterial communities during sewage sludge composting","volume":"49","author":"Su","year":"2015","journal-title":"Environ. Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.envpol.2014.01.015","article-title":"Occurrence, sources, and fate of pharmaceuticals in aquatic environment and soil","volume":"187","author":"Li","year":"2014","journal-title":"Environ. Pollut."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/j.jenvman.2016.07.049","article-title":"Removal of pharmaceuticals and personal care products (PPCPs) from wastewater: A review","volume":"182","author":"Wang","year":"2016","journal-title":"J. Environ. Manag."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.scitotenv.2017.04.102","article-title":"Occurrences and removal of pharmaceuticals and personal care products (PPCPs) in drinking water and water\/sewage treatment plants: A review","volume":"596\u2013597","author":"Yang","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_14","first-page":"11","article-title":"Ranking of concern, based on environmental indexes, for pharmaceutical and personal care products: An application to the Spanish case","volume":"135","year":"2014","journal-title":"J. Environ. Manag."},{"key":"ref_15","first-page":"71","article-title":"Occurrence of pesticides and PPCPs in surface and drinking water in southern Brazil: Data on 4-Year Monitoring","volume":"30","author":"Caldas","year":"2018","journal-title":"J. Braz. Chem. Soc."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"133925","DOI":"10.1016\/j.jhazmat.2024.133925","article-title":"Occurrence, fate, and ecological risk of antibiotics in wastewater treatment plants in China: A review","volume":"469","author":"Wang","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.chemosphere.2008.11.086","article-title":"Antibiotics in the aquatic environment: A review","volume":"75","year":"2009","journal-title":"Chemosphere"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"121067","DOI":"10.1016\/j.jhazmat.2019.121067","article-title":"Determination of selected antiretroviral drugs in wastewater, surface water and aquatic plants using hollow fibre liquid phase microextraction and LC-MS\/MS","volume":"382","author":"Mlunguza","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"111039","DOI":"10.1016\/j.molliq.2019.111039","article-title":"High performance removal and simulation studies of diuron pesticide in water on MWCNTs","volume":"289","author":"Ali","year":"2019","journal-title":"J. Mol. Liq."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/bs.ache.2024.12.002","article-title":"Performances of conventional treatment techniques for the treatment of pharmaceutical contaminated water: Challenges and opportunities","volume":"Volume 65","author":"Purkait","year":"2025","journal-title":"Advances in Chemical Engineering"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.micromeso.2018.01.013","article-title":"Sorption of diclofenac onto functionalized mesoporous silicas: Experimental and theoretical investigations","volume":"264","author":"Barczak","year":"2018","journal-title":"Microporous Mesoporous Mater."},{"key":"ref_22","first-page":"100504","article-title":"Biochar for wastewater treatment: Addressing contaminants and enhancing sustainability: Challenges and solutions","volume":"16","author":"Alshehri","year":"2024","journal-title":"J. Hazard. Mater. Adavances"},{"key":"ref_23","first-page":"634","article-title":"Membrane bioreactors for hospital wastewater treatment: Recent advancements in membranes and processes","volume":"16","author":"Zhao","year":"2021","journal-title":"Front. CSE"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.chemosphere.2017.11.165","article-title":"Removal of veterinary antibiotics from wastewater by electrocoagulation","volume":"194","author":"Baran","year":"2018","journal-title":"Chemosphere"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"109358","DOI":"10.1016\/j.jece.2023.111558","article-title":"A review on the treatment of water and wastewater by electrocoagulation process: Advances and emerging applications","volume":"11","author":"Bharti","year":"2023","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_26","first-page":"e30402","article-title":"Advanced oxidation processes for water and wastewater treatment\u2014Guidance for systematic future research","volume":"10","author":"Spahr","year":"2024","journal-title":"Chem. Eng. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.jphotochem.2016.08.029","article-title":"Enhanced photocatalytic degradation of atenolol using graphene TiO2 composite","volume":"332","author":"Bhatia","year":"2017","journal-title":"J. Photochem. Photobiol. A Chem."},{"key":"ref_28","first-page":"100123","article-title":"Photocatalytic degradation of pharmaceuticals: Insights into biochar modification and degradation mechanism","volume":"2","author":"Bhattacharjee","year":"2024","journal-title":"Next Mater."},{"key":"ref_29","first-page":"100432","article-title":"Recent advances in round-the-clock photocatalytic system: Mechanisms, characterization techniques and applications","volume":"48","author":"Cai","year":"2021","journal-title":"J. Photochem. Photobiol. C Photochem. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0926-3373(97)00059-3","article-title":"Aniline mineralization by AOP\u2019s: Anodic oxidation, photocatalysis, electro-Fenton and photoelectro-Fenton processes","volume":"16","author":"Brillas","year":"1998","journal-title":"Appl. Catal. B Environ."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Pa\u00edga, P., Figueiredo, S., Correia, M., Andr\u00e9, M., Barbosa, R., Jorge, S., and Delerue-Matos, C. (2025). Occurrence of 97 Pharmaceuticals in Wastewater and Receiving Waters: Analytical Validation and Treatment Influence. J. Xenobiot., 15.","DOI":"10.3390\/jox15030078"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1007\/s10661-025-14269-y","article-title":"Pharmaceuticals in drinking water in suburban communities in Chengdu, China: Potential risks on the human health","volume":"197","author":"Tang","year":"2025","journal-title":"Environ. Monit. Assess."},{"key":"ref_33","first-page":"651","article-title":"Quality survey of natural mineral water and spring water sold in France: Monitoring of hormones, pharmaceuticals, pesticides, perfluoroalkyl substances, phthalates, and alkylphenols at the ultra-trace level","volume":"603\u2013604","author":"Labadie","year":"2017","journal-title":"Sci. T.T.E."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Farhan, M., Hasani, I.W., Khafaga, D.S.R., Ragab, W.M., Ahmed Kazi, R.N., Aatif, M., Muteeb, G., and Fahim, Y.A. (2025). Enzymes as Catalysts in Industrial Biocatalysis: Advances in Engineering, Applications, and Sustainable Integration. Catalysts, 15.","DOI":"10.3390\/catal15090891"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2204","DOI":"10.1007\/s10562-019-02821-8","article-title":"Tailoring Multipurpose Biocatalysts via Protein Engineering Approaches: A Review","volume":"149","author":"Bilal","year":"2019","journal-title":"Catal. Lett."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"561","DOI":"10.2174\/0929866524666170404162730","article-title":"Impact of Salt Concentration and pH on Surface Charged Residues: Controlling Protein Association Pathways in Carboxylesterase EstGtA2","volume":"24","author":"Moisan","year":"2017","journal-title":"Protein Pept. Lett."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1016\/j.biortech.2005.06.017","article-title":"Impact of reaction conditions on the laccase-catalyzed conversion of bisphenol A","volume":"97","author":"Kim","year":"2006","journal-title":"Bioresour. Technol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijbiomac.2012.11.024","article-title":"Effect of pH and temperature on stability and kinetics of novel extracellular serine alkaline protease (70 kDa)","volume":"54","author":"Bhunia","year":"2013","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"134390","DOI":"10.1016\/j.chemosphere.2022.134390","article-title":"Advances in the application of immobilized enzyme for the remediation of hazardous pollutant: A review","volume":"299","author":"Yaashikaa","year":"2022","journal-title":"Chemosphere"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1002\/elsc.201900119","article-title":"Kinetic and thermodynamic properties of purified alkaline protease from Bacillus pumilus Y7 and non-covalent immobilization to poly(vinylimidazole)\/clay hydrogel","volume":"20","author":"Duman","year":"2020","journal-title":"Eng. Life Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.eng.2025.03.033","article-title":"Modular Engineering of a Synthetic Biology-Based Platform for Sustainable Bioremediation of Residual Antibiotics in Aquatic Environments","volume":"53","author":"Ren","year":"2025","journal-title":"Engineering"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1021\/cbe.4c00101","article-title":"Recyclable Enzymatic Hydrolysis with Metal-Organic Framework Stabilized Humicola insolens Cutinase (HiC) for Potential PET Upcycling","volume":"1","author":"Wu","year":"2024","journal-title":"Chem. Bio. Eng."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Noman, E., Al-Gheethi, A.A., Talip, B.A., Mohamed, R., and Kassim, A.H. (2020). Oxidative Enzymes from Newly Local Strain Aspergillus iizukae EAN605 Using Pumpkin Peels as a Production Substrate: Optimized Production, Characterization, Application and Techno-Economic Analysis, Elsevier.","DOI":"10.1016\/j.jhazmat.2019.121954"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4767","DOI":"10.1007\/s12010-025-05267-4","article-title":"Self-assembled Immobilization and Metal-Polyphenol Network Encapsulation of \u03b2-Galactosidase on T4 phage for Enhanced Biocatalytic Performance","volume":"197","author":"Wu","year":"2025","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1016\/j.ijbiomac.2017.04.033","article-title":"Production, immobilization and thermodynamic studies of free and immobilized Aspergillus awamori amylase","volume":"102","author":"Karam","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e01121-25","DOI":"10.1128\/spectrum.01121-25","article-title":"Thermostable and recyclable Candida antarctica lipase B immobilized on Bacillus subtilis using sortase technology","volume":"13","author":"Ghaedmohammadi","year":"2025","journal-title":"Microbiol. Spectr."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1016\/j.watres.2006.02.014","article-title":"Biological degradation of pharmaceuticals in municipal wastewater treatment: Proposing a classification scheme","volume":"40","author":"Joss","year":"2006","journal-title":"Water Res."},{"key":"ref_48","first-page":"118799","article-title":"Bottom-up artificial construction of the M7-Z4 bacterial model enhanced sulfamethazine mineralization: Metagenomic analysis combined with bacterial isolation techniques","volume":"13","author":"Hu","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_49","first-page":"643","article-title":"Degradation kinetics of pharmaceuticals and personal care products in surface waters: Photolysis vs biodegradation","volume":"590\u2013591","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e00884-24","DOI":"10.1128\/msystems.00884-24","article-title":"Interspecies synergistic interactions mediated by cofactor exchange enhance stress tolerance by inducing biofilm formation","volume":"9","author":"Wang","year":"2024","journal-title":"mSystems"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Chen, X.Y., Zhang, X., Zhao, X.Y., Xi, B.D., and Lu, Y. (2025). Microbial co-metabolism in the degradation of emerging organic pollutants. J. Environ. Sci.","DOI":"10.1016\/j.jes.2025.12.002"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"124725","DOI":"10.1016\/j.watres.2025.124725","article-title":"Photocatalysis-fueled algae-bacteria mutualism enables P450 enzyme-induced nitrite-free anammox for sustainable nitrogen removal","volume":"288","author":"Dong","year":"2026","journal-title":"Water Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"119973","DOI":"10.1016\/j.envres.2024.119973","article-title":"Development of oriented microbial consortium-based compound enzyme strengthens food waste hydrolysis and antibiotic resistance genes removal: Deciphering of performance, metabolic pathways and microbial communities","volume":"262","author":"Cui","year":"2024","journal-title":"Environ. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5510","DOI":"10.1038\/s41467-024-49831-8","article-title":"Co-immobilization of whole cells and enzymes by covalent organic framework for biocatalysis process intensification","volume":"15","author":"Zheng","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"133224","DOI":"10.1016\/j.biortech.2025.133224","article-title":"Designing a mesoporous cascade reactor for enhanced enzymatic performance","volume":"438","author":"Chen","year":"2025","journal-title":"Bioresour. Technol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"131177","DOI":"10.1016\/j.biortech.2024.131177","article-title":"Bacterial surface display of PETase mutants and MHETase for an efficient dual-enzyme cascade catalysis","volume":"408","author":"Xue","year":"2024","journal-title":"Bioresour. Technol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Huang, L., Jin, Y., Zhou, D.H., Liu, L.X., Huang, S.K., Zhao, Y.Q., and Chen, Y.C. (2022). A Review of the Role of Extracellular Polymeric Substances (EPS) in Wastewater Treatment Systems. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph191912191"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"168713","DOI":"10.1016\/j.cej.2025.168713","article-title":"Multi-omics insights into ROS-mediated molecular responses of extracellular polymeric substances in aerobic granular sludge under micro\/nanoplastic stress","volume":"523","author":"Li","year":"2025","journal-title":"Chem. Eng. J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e2300744","DOI":"10.1002\/biot.202300744","article-title":"Design of a cofactor self-sufficient whole-cell biocatalyst for enzymatic asymmetric reduction via engineered metabolic pathways and multi-enzyme cascade","volume":"19","author":"Zou","year":"2024","journal-title":"Biotechnol. J."},{"key":"ref_60","first-page":"108533","article-title":"Co-immobilization of lipase and laccase on agarose-based supports via layer-by-layer strategy: Effect of diffusional limitations","volume":"186","author":"Rios","year":"2022","journal-title":"Biochem. Eng. J."},{"key":"ref_61","first-page":"134828","article-title":"Spatially sequential co-immobilization of phosphorylases in tiny environments and its application in the synthesis of glucosyl glycerol","volume":"278","author":"Yang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"122627","DOI":"10.1016\/j.watres.2024.122627","article-title":"A single-atom manganese nanozyme mediated membrane reactor for water decontamination","volume":"268","author":"Sun","year":"2025","journal-title":"Water Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"127503","DOI":"10.1016\/j.jhazmat.2021.127755","article-title":"A hybrid nanobiocatalyst with in situ encapsulated enzyme and exsolved Co nanoclusters for complete chemoenzymatic conversion of methyl parathion to 4-aminophenol","volume":"424","author":"Wang","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"137454","DOI":"10.1016\/j.jhazmat.2025.137454","article-title":"Photo-enzyme cascade catalysis treatment of bisphenol A in water: Synergistic hydroxylation pathway for mineralization and detoxification","volume":"489","author":"Du","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_65","first-page":"158116","article-title":"In-situ assembled biochar-yeast hybrid for tetracycline detoxification: Unparalleled robustness, toxicity assessment, and multi-omics analysis","volume":"503","author":"Wang","year":"2025","journal-title":"Chem. Eng. J."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.jiec.2024.08.015","article-title":"Assessing the environmental risk potential of transformation byproducts formed during fungal enzymatic treatment of a pharmaceutical mixture","volume":"142","author":"Oh","year":"2025","journal-title":"J. Ind. Eng. Chem."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"134716","DOI":"10.1016\/j.jhazmat.2024.134716","article-title":"Two different types of hydrolases co-degrade ochratoxin A in a highly efficient degradation strain Lysobacter sp. CW239","volume":"473","author":"Fu","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_68","first-page":"CNKI","article-title":"A Comparative Study on Several Carriers of Immobilized Cells","volume":"2","author":"Jiang","year":"1993","journal-title":"Environ. Sci. Resour. Util."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1016\/j.chempr.2018.03.001","article-title":"Hierarchically Engineered Mesoporous Metal-Organic Frameworks toward Cell-free Immobilized Enzyme Systems","volume":"4","author":"Li","year":"2018","journal-title":"Chem"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Chen, T.P., Wang, S.M., Niu, H.Q., Yang, G.J., Wang, S.N., Wang, Y.Q., Zhou, C.W., Yu, B., Yang, P.P., and Sun, W.J. (2023). Biofilm-Based Biocatalysis for Galactooligosaccharides Production by the Surface Display of \u03b2-Galactosidase in Pichia pastoris. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24076507"},{"key":"ref_71","first-page":"100077","article-title":"Biofilm formation and its implications on the properties and fate of microplastics in aquatic environments: A review","volume":"6","author":"Sooriyakumar","year":"2022","journal-title":"J. Hazard. Mater. Adv."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1038\/s41579-022-00791-0","article-title":"The biofilm matrix: Multitasking in a shared space","volume":"21","author":"Flemming","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1016\/j.tibtech.2024.11.002","article-title":"Engineering biology approaches to modulate bacterial biofilms","volume":"43","author":"Carr","year":"2025","journal-title":"Trends Biotechnol."},{"key":"ref_74","first-page":"6123","article-title":"An antibiotic-degrading engineered biofilm platform to combat environmental antibiotic resistance","volume":"10","author":"Ahan","year":"2024","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1007\/s11427-022-2214-2","article-title":"Enabling technology and core theory of synthetic biology","volume":"66","author":"Zhang","year":"2023","journal-title":"Sci. China Life Sci."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1021\/acssynbio.4c00793","article-title":"Escherichia coli Surface Display: Advances and Applications in Biocatalysis","volume":"14","author":"Liu","year":"2025","journal-title":"ACS Synth. Biol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1021\/acssynbio.1c00183","article-title":"Engineering a supersecreting strain of Escherichia coli by directed coevolution of the multiprotein TAT translocation machinery","volume":"10","author":"Taw","year":"2021","journal-title":"ACS Synth. Biol."},{"key":"ref_78","first-page":"e00023-25","article-title":"Genetically engineered bacteria and microalgae expressing a mutant of cytochrome P450 BM3 for efficient Diuron degradation in wastewater treatment","volume":"91","author":"Helvig","year":"2025","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1042\/BST20230229","article-title":"Bacterial microcompartments as a next-generation metabolic engineering tool: Utilizing nature\u2019s solution for confining challenging catabolic pathways","volume":"52","author":"Doron","year":"2024","journal-title":"Biochem. Soc. Trans."},{"key":"ref_80","first-page":"70","article-title":"A critical review of pharmaceutical pollutants in soil and air: Ecotoxicological impacts on animal, plant and microbial communities\u2014Health hazards and waste management","volume":"3","author":"Amin","year":"2026","journal-title":"J. Hazard. Mater. Adv."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"130328","DOI":"10.1016\/j.biortech.2024.130328","article-title":"Decomposition of non-steroidal anti-inflammatory drugs by activated sludge supported by biopreparation in sequencing batch reactor","volume":"395","author":"Dzionek","year":"2024","journal-title":"Bioresour. Technol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"104147","DOI":"10.1016\/j.jwpe.2023.104023","article-title":"Eradication of ibuprofen and diclofenac via in situ synthesized and immobilized bacterial laccase to Cu-based metal organic framework","volume":"54","author":"Bhardwaj","year":"2023","journal-title":"J. Water Process Eng."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"177412","DOI":"10.1016\/j.scitotenv.2024.177412","article-title":"Acyclovir contamination in environment: Occurrence, transformation, toxicity, risk, and evaluation as a pharmaceutical indicator","volume":"957","author":"Peng","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.hydres.2022.11.001","article-title":"The pharmaceutical disposal practices and environmental contamination: A review in East African countries","volume":"5","author":"Karungamye","year":"2022","journal-title":"HydroResearch"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Bernardes, M.F.F., Pazin, M., Pereira, L.C., and Dorta, D.J. (2015). Impact of Pesticides on Environmental and Human Health, Intechopen.","DOI":"10.5772\/59710"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Daughton, C.G. (2007). Pharmaceuticals in the environment: Sources and their management. Comprehensive Analytical Chemistry, Elsevier.","DOI":"10.1016\/S0166-526X(07)50001-2"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"125","DOI":"10.18231\/j.ijcaap.13328.1761800153","article-title":"Green and sustainable pharmacology: Integrating environmental responsibility into drug discovery, development, and practice","volume":"10","author":"Dube","year":"2025","journal-title":"IP Int. J. Compr. Adv. Pharmacol."},{"key":"ref_88","first-page":"2427","article-title":"Designing at-source and end-of-pipe biotechnologies to tackle pharmaceutical pollution","volume":"43","author":"Akhrimenko","year":"2025","journal-title":"Trends Biotechnol."},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Parker, G., and Miller, F.A. (2024). Tackling Pharmaceutical Pollution Along the Product Lifecycle: Roles and Responsibilities for Producers, Regulators and Prescribers. Pharmacy, 12.","DOI":"10.3390\/pharmacy12060173"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"3115","DOI":"10.1007\/s10311-021-01194-y","article-title":"How to decrease pharmaceuticals in the environment? A review","volume":"19","author":"Caban","year":"2021","journal-title":"Environ. Chem. Lett."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"547","DOI":"10.4103\/0253-7613.100363","article-title":"Ecopharmacovigilance: An issue urgently to be addressed","volume":"44","author":"Medhi","year":"2012","journal-title":"Indian J. Pharmacol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"118","DOI":"10.56012\/rlhx7717","article-title":"Ecopharmacovigilance: A review of cause, impact, and remedies","volume":"32","author":"Dzidzornu","year":"2023","journal-title":"Med. Writ."},{"key":"ref_93","unstructured":"WHO (2026, May 08). One Health. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/one-health."},{"key":"ref_94","unstructured":"EU (2019, January 07). European Parliament Resolution on a Strategic Approach to Pharmaceuticals in the Environment. Available online: https:\/\/www.europarl.europa.eu\/doceo\/document\/TA-9-2020-0226_EN.html."},{"key":"ref_95","unstructured":"OECD (2019). Pharmaceutical Residues in Freshwater: Hazards and Policy Responses, OECD Publishing."},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Stefanache, A., Marcinschi, A., Marin, G.A., Mitran, A.M., Lungu, I.I., Miftode, A.M., Crivoi, F., Lacatusu, D., Baican, M., and Cioanca, O. (2025). Green Chemistry Approaches in Pharmaceutical Synthesis: Sustainable Methods for Drug Development. AppliedChem, 5.","DOI":"10.3390\/appliedchem5020013"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.tibtech.2024.10.020","article-title":"Complex waste stream valorization through combined enzymatic hydrolysis and catabolic assimilation by Pseudomonas putida","volume":"43","author":"Gosalvitr","year":"2025","journal-title":"Trends Biotechnol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1007\/s42773-025-00465-z","article-title":"Conversation of pesticide residues into ammonium nitrogen (NH4+-N) through AOPs and its fertilization effect on lettuce growth","volume":"7","author":"He","year":"2025","journal-title":"Biochar"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"145555","DOI":"10.1016\/j.foodchem.2025.145555","article-title":"pH and CO2\/N2 dual responsive Pickering emulsion stabilized by shellac nanoparticle-enzyme conjugates for synthesis of phytosterol esters","volume":"493","author":"Jiang","year":"2025","journal-title":"Food Chem."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.ymben.2017.09.013","article-title":"Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol","volume":"44","author":"Wang","year":"2017","journal-title":"Metab. Eng."},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Mehta, N. (2026). The synthetic microbial ecosystem: Rational design, dynamic control, and translational impact of consortia for sustainable waste valorization. ChemRxiv.","DOI":"10.26434\/chemrxiv.15001362\/v1"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"144278","DOI":"10.1016\/j.ijbiomac.2025.144278","article-title":"Enhancing industrial biocatalyst performance and cost-efficiency through adsorption-based enzyme immobilization: A review","volume":"316","author":"Ishak","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/0167-8809(88)90076-X","article-title":"Environmental and ecological problems in the introduction of alien microorganisms in the soil","volume":"24","author":"Fisher","year":"1988","journal-title":"Agric. Ecosyst. Environ."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1038\/s41579-021-00650-4","article-title":"Horizontal gene transfer and adaptive evolution in bacteria","volume":"20","author":"Arnold","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.3390\/biomass4040062","article-title":"Biomass Demineralization and Pretreatment Strategies to Reduce Inhibitor Concentrations in Itaconic Acid Fermentation by Aspergillus terreus","volume":"4","author":"Kennedy","year":"2024","journal-title":"Biomass"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"e2508001","DOI":"10.4014\/jmb.2508.08001","article-title":"Governing Synthetic Biology: A Co-Evolutionary Framework for Sustainable Innovation","volume":"35","author":"Kim","year":"2025","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Salzano, D., Shannon, B., Grierson, C., Marucci, L., Savery, N.J., and di Bernardo, M. (2024). In-vivo distributed multicellular control of gene expression in microbial consortia. bioRxiv.","DOI":"10.1101\/2023.11.13.566807"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"L\u00e1zaro, H.M., Otero-Muras, I., and Carbonell, P. (2025). BiosInt: Biosensor-based smart design of pathway dynamic regulation for industrial biomanufacturing. bioRxiv.","DOI":"10.1101\/2025.11.17.688676"},{"key":"ref_109","unstructured":"Hsieh, Y.L. (2026). Computational Integration of Multi-Omics and Phenotype Data Into Genome-Scale Metabolic Models, Universit\u00e4t Potsdam."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Molina Ortiz, J.P., McClure, D.D., Holmes, A., Rice, S.A., Read, M.N., and Shanahan, E.R. (2024). Genome scale metabolic modelling of human gut microbes to inform rational community design. bioRxiv.","DOI":"10.1101\/2024.05.28.596116"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1038\/s41586-025-08947-7","article-title":"Bioremediation of complex organic pollutants by engineered Vibrio natriegens","volume":"642","author":"Su","year":"2025","journal-title":"Nature"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1038\/s41587-022-01618-2","article-title":"Large language models generate functional protein sequences across diverse families","volume":"41","author":"Madani","year":"2023","journal-title":"Nat. Biotechnol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"103","DOI":"10.2174\/0122133461369309250409164858","article-title":"Biotechnological Advancements in Active Pharmaceutical Ingredient Removal: Sustainable Solutions for Pharmaceutical Wastewater Treatment","volume":"13","author":"Chauhan","year":"2026","journal-title":"Curr. Green Chem."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"125564","DOI":"10.1016\/j.jenvman.2025.125564","article-title":"Biodegradation of pharmaceutical contaminants in wastewater using microbial consortia: Mechanisms, applications, and challenges","volume":"384","author":"Sesay","year":"2025","journal-title":"J. Environ. Manag."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/10\/804\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T03:23:24Z","timestamp":1779679404000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/10\/804"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,19]]},"references-count":114,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["biology15100804"],"URL":"https:\/\/doi.org\/10.3390\/biology15100804","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,19]]}}}