{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:41:09Z","timestamp":1785415269502,"version":"3.56.0"},"reference-count":36,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002842","name":"Chiang Mai University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002842","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/05m2fqn25","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Thai shallot (Allium cepa L. var. aggregatum) is rich in quercetin glycosides, which can be enzymatically hydrolyzed into aglycone forms with potentially higher bioavailability. However, whether this structural modification enhances metabolic efficacy in vivo remains unclear. This study aimed to compare the metabolic and histopathological effects of enzymatic (ESE) and non-enzymatic (NES) Thai shallot extracts in a high-fat diet (HFD)-induced obese mouse model. Male C57BL\/6 mice were fed HFD for 12 weeks to induce obesity, followed by a 12-week treatment with NES or ESE (1000 and 2000 mg\/kg\/day). Metabolic parameters, lipid profiles, oxidative stress markers, hepatic enzyme activities, and histopathological changes were evaluated. Enzymatic hydrolysis significantly increased the proportion of quercetin aglycone without altering total quercetin content. Both NES and ESE improved fasting glucose, insulin resistance, lipid profiles, and oxidative stress markers compared with HFD controls. Histological examination showed attenuation of hepatic steatosis and preservation of tissue architecture in treated groups. However, no consistent superiority of ESE over NES was observed across metabolic or histopathological outcomes. Despite substantial modification of flavonoid composition, enzymatic processing did not enhance the measured metabolic efficacy of Thai shallot extract under the conditions tested. Because circulating quercetin and metabolite levels were not assessed, this finding should be interpreted as comparable metabolic efficacy rather than evidence of equivalent bioavailability. These findings suggest that factors beyond aglycone content may play a key role in determining biological activity, with implications for the development and cost-effectiveness of functional food products.<\/jats:p>","DOI":"10.3390\/biology15130995","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T16:50:33Z","timestamp":1782924633000},"page":"995","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparable Metabolic and Histopathological Observations of Enzymatic and Non-Enzymatic Thai Shallot Extracts in High-Fat Diet-Induced Obese Mice"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0176-2475","authenticated-orcid":false,"given":"Artorn","family":"Anuduang","sequence":"first","affiliation":[{"name":"Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0367-9752","authenticated-orcid":false,"given":"Jiraporn","family":"Laoung-on","sequence":"additional","affiliation":[{"name":"Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3633-9255","authenticated-orcid":false,"given":"Oranit","family":"Kraseasintra","sequence":"additional","affiliation":[{"name":"Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-8781","authenticated-orcid":false,"given":"Somdet","family":"Srichairatanakool","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kittipan","family":"Rerkasem","sequence":"additional","affiliation":[{"name":"Environmental\u2014Occupational Health Sciences and Non Communicable Diseases Center of Excellence, 13 Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9067-2051","authenticated-orcid":false,"given":"Sakaewan","family":"Ounjaijean","sequence":"additional","affiliation":[{"name":"Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1429-7709","authenticated-orcid":false,"given":"Kongsak","family":"Boonyapranai","sequence":"additional","affiliation":[{"name":"Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/s13679-015-0169-4","article-title":"Overweight and Obesity: Prevalence, Consequences, and Causes of a Growing Public Health Problem","volume":"4","author":"Williams","year":"2015","journal-title":"Curr. Obes. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"19556","DOI":"10.1038\/s41598-019-55987-x","article-title":"Effects of different diets used in diet-induced obesity models on insulin resistance and vascular dysfunction in C57BL\/6 mice","volume":"9","author":"Lang","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1194\/jlr.M038786","article-title":"Quercetin reduces obesity-associated ATM infiltration and inflammation in mice: A mechanism including AMPK\u03b11\/SIRT1","volume":"55","author":"Dong","year":"2014","journal-title":"J. Lipid Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5352","DOI":"10.1002\/ptr.7144","article-title":"Quercetin and metabolic syndrome: A review","volume":"35","author":"Hosseini","year":"2021","journal-title":"Phytother. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.chroma.2005.12.016","article-title":"The analysis of onion and garlic","volume":"1112","author":"Lanzotti","year":"2006","journal-title":"J. Chromatogr. A"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4409","DOI":"10.1002\/fsn3.3426","article-title":"Antiobesity effects of onion (Allium cepa) in subjects with obesity: Systematic review and meta-analysis","volume":"11","author":"Chung","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_7","first-page":"3","article-title":"The effects of Allium cepa L. (onion) and its active constituents on metabolic syndrome: A review","volume":"24","author":"Galavi","year":"2021","journal-title":"Iran. J. Basic Med. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Laoung-on, J., Saenjum, C., Boonyapranai, K., and Ounjaijean, S. (2026). Antioxidant and Anti-Inflammatory Effect of Thai Shallot (Allium ascalonicum cv. chiangmai) and Cha-Miang (Camellia sinensis var. assamica) Extracts on Human Embryonic Kidney Cell Line (HEK293). Life, 16.","DOI":"10.3390\/life16010141"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Laoung-on, J., Anuduang, A., Saenjum, C., Srichairatanakool, S., Boonyapranai, K., and Ounjaijean, S. (2025). Anti-diabetic and antioxidant effects of Thai shallot in animal models. Biology, 14.","DOI":"10.3390\/biology14060627"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1093\/jn\/138.5.885","article-title":"Quercetin from shallots (Allium cepa L. var. aggregatum) is more bioavailable than its glucosides","volume":"138","author":"Wiczkowski","year":"2008","journal-title":"J. Nutr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1093\/jn\/130.5.1200","article-title":"Bioavailabilities of Quercetin-3-Glucoside and Quercetin-4\u2032-Glucoside Do Not Differ in Humans","volume":"130","author":"Olthof","year":"2000","journal-title":"J. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kothari, D., Lee, W.D., and Kim, S.K. (2020). Allium flavonols: Health benefits and bioavailability. Antioxidants, 9.","DOI":"10.3390\/antiox9090888"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.48048\/wjst.2020.10708","article-title":"Preliminary study on hepatoprotective activity of aqueous crude extract of Allium ascalonicum against ethanol-induced liver injury in mice","volume":"17","author":"Ounjaijean","year":"2020","journal-title":"Walailak J. Sci. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"S215","DOI":"10.2337\/diabetes.53.suppl_3.S215","article-title":"The high-fat diet-fed mouse: A model for studying mechanisms and treatment of impaired glucose tolerance and type 2 diabetes","volume":"53","author":"Winzell","year":"2004","journal-title":"Diabetes"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1038\/oby.2007.608","article-title":"High-fat diets: Modeling metabolic disorders of human obesity in rodents","volume":"15","author":"Buettner","year":"2007","journal-title":"Obesity"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1016\/j.cell.2012.02.017","article-title":"Mechanisms for insulin resistance: Common threads and missing links","volume":"148","author":"Samuel","year":"2012","journal-title":"Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1752","DOI":"10.1172\/JCI21625","article-title":"Increased oxidative stress in obesity and its impact on metabolic syndrome","volume":"114","author":"Furukawa","year":"2004","journal-title":"J. Clin. Investig."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3313","DOI":"10.1007\/s00018-018-2860-6","article-title":"Molecular mechanisms of hepatic lipid accumulation in non-alcoholic fatty liver disease","volume":"75","author":"Ipsen","year":"2018","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_19","first-page":"12345","article-title":"Health Benefits of the Flavonoids from Onion: Constituents and Their Pronounced Antioxidant and Anti-neuroinflammatory Capacities","volume":"68","author":"Li","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1002\/mnfr.201000392","article-title":"Chronic dietary intake of quercetin alleviates hepatic fat accumulation associated with consumption of a Western-style diet in C57\/BL6J mice","volume":"55","author":"Kobori","year":"2011","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1007\/s12263-014-0418-2","article-title":"Quercetin decreases high-fat diet induced body weight gain and accumulation of hepatic and circulating lipids in mice","volume":"9","author":"Swarts","year":"2014","journal-title":"Genes Nutr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"108532","DOI":"10.1016\/j.jnutbio.2020.108532","article-title":"Effect of quercetin on nonshivering thermogenesis of brown adipose tissue in high-fat diet-induced obese mice","volume":"88","author":"Pei","year":"2021","journal-title":"J. Nutr. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"438","DOI":"10.4162\/nrp.2023.17.3.438","article-title":"Quercetin inhibits body weight gain and adipogenesis via matrix metalloproteinases in mice fed a high-fat diet","volume":"17","author":"Song","year":"2023","journal-title":"Nutr. Res. Pract."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Tan, Y., Tam, C.C., Rolston, M., Alves, P., Chen, L., Meng, S., Hong, H., Chang, S.K.C., and Yokoyama, W. (2021). Quercetin ameliorates insulin resistance and restores gut microbiome in mice on high-fat diets. Antioxidants, 10.","DOI":"10.3390\/antiox10081251"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1080\/13880209.2021.1874028","article-title":"A review of anti-inflammatory, antioxidant, and immunomodulatory effects of Allium cepa and its main constituents","volume":"59","author":"Marefati","year":"2021","journal-title":"Pharm. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.clnu.2011.01.010","article-title":"Quercetin reduces markers of oxidative stress and inflammation in sarcoidosis","volume":"30","author":"Boots","year":"2011","journal-title":"Clin. Nutr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"71","DOI":"10.3892\/br.2012.27","article-title":"Quercetin improves insulin resistance and hepatic lipid accumulation in vitro in a NAFLD cell model","volume":"1","author":"Li","year":"2013","journal-title":"Biomed. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1161\/CIRCULATIONAHA.111.067264","article-title":"Body fat distribution and risk of cardiovascular disease: An update","volume":"126","year":"2012","journal-title":"Circulation"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1093\/ajcn\/62.6.1276","article-title":"Absorption of dietary quercetin glycosides and quercetin in healthy ileostomy volunteers","volume":"62","author":"Hollman","year":"1995","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S0014-5793(97)01367-7","article-title":"Relative bioavailability of the antioxidant flavonoid quercetin from various foods in man","volume":"418","author":"Hollman","year":"1997","journal-title":"FEBS Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1093\/jn\/138.9.1615","article-title":"Daily quercetin supplementation dose-dependently increases plasma quercetin concentrations in healthy humans","volume":"138","author":"Egert","year":"2017","journal-title":"J. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6485","DOI":"10.1021\/jf902107d","article-title":"Interaction between phenolics and gut microbiota: Role in human health","volume":"57","author":"Selma","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1080\/19490976.2016.1158395","article-title":"Bacterial species involved in the conversion of dietary flavonoids in the human gut","volume":"7","author":"Braune","year":"2016","journal-title":"Gut Microbes"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Ozdal, T., Sela, D.A., Xiao, J., Boyacioglu, D., Chen, F., and Capanoglu, E. (2016). The reciprocal interactions between polyphenols and gut microbiota and effects on bioaccessibility. Nutrients, 6.","DOI":"10.3390\/nu8020078"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s11101-008-9095-3","article-title":"Microbial metabolism of dietary phenolic compounds in the colon","volume":"7","author":"Aura","year":"2008","journal-title":"Phytochem. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1039\/C4FO01178C","article-title":"Quercetin and related polyphenols: New insights and implications for their bioactivity and bioavailability","volume":"6","author":"Kawabata","year":"2015","journal-title":"Food Funct."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/13\/995\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T04:15:18Z","timestamp":1783052118000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/13\/995"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,24]]},"references-count":36,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["biology15130995"],"URL":"https:\/\/doi.org\/10.3390\/biology15130995","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,24]]}}}