{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:17:16Z","timestamp":1785421036080,"version":"3.56.0"},"reference-count":81,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["126020216227-3"],"award-info":[{"award-number":["126020216227-3"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]},{"award":["126020216227-3"],"award-info":[{"award-number":["126020216227-3"]}],"id":[{"id":"https:\/\/ror.org\/00ghqgy32","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Mass spectrometry and high-throughput sequencing have been introduced into clinical bacteriology. We characterized strain IMB-1, previously isolated from the cerebrospinal fluid of a child, as Pseudoxanthomonas kaohsiungensis and analyzed its biological properties, resistance phenotype, and complete genome. The IMB-1 strain displayed amylolytic, weak lipolytic activities, and it exhibited a phenotypic resistance profile only for aminoglycosides. The dDDH calculation based on the complete genome sequence showed that strain IMB-1 was closely grouped with the type strain P. kaohsiungensis DSM 17583, and the dDDH (d4) value was 70.1%. A comparative pan-genome analysis was performed for four P. kaohsiungensis genomes, revealing a substantial shared core genome. The IMB-1 genome contained 508 unique gene clusters, representing the largest strain-specific gene set among the analyzed genomes, suggesting genomic plasticity and adaptation to the host-associated environment. Genome annotation revealed genes responsible for antibiotic, disinfecting agent, and antiseptic resistance. Gene clusters exhibiting the potential to form biofilms, adhere to the epithelial surface, and exhibit resistance to stress factors were identified. Our study demonstrates that strain IMB-1 is a potential opportunistic pathogen with significant pathogenic potential. The application of high-resolution whole-genome sequencing data in public health for pathogen identification and monitoring can improve the accuracy of infection source determination, reduce the scale and burden of outbreaks, and identify and quantify antimicrobial resistance in pathogens.<\/jats:p>","DOI":"10.3390\/biology15131010","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:49:23Z","timestamp":1782434963000},"page":"1010","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pathogenic Potential of Pseudoxanthomonas kaohsiungensis Strain IMB-1 Based on Whole-Genome Sequencing"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9720-068X","authenticated-orcid":false,"given":"Natalia","family":"Belkova","sequence":"first","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u2018Scientific Centre for Family Health and Human Reproduction Problems\u2019, Epidemiology and Microbiology Institute, 3, K. Marks Str., 664003 Irkutsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3614-6631","authenticated-orcid":false,"given":"Nadezhda","family":"Smurova","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u2018Scientific Centre for Family Health and Human Reproduction Problems\u2019, Epidemiology and Microbiology Institute, 3, K. Marks Str., 664003 Irkutsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8522-7359","authenticated-orcid":false,"given":"Raisa","family":"Zugeeva","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u2018Scientific Centre for Family Health and Human Reproduction Problems\u2019, Epidemiology and Microbiology Institute, 3, K. 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Marks Str., 664003 Irkutsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Dorzhieva","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u2018Scientific Centre for Family Health and Human Reproduction Problems\u2019, Epidemiology and Microbiology Institute, 3, K. Marks Str., 664003 Irkutsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7656-342X","authenticated-orcid":false,"given":"Uliana","family":"Nemchenko","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u2018Scientific Centre for Family Health and Human Reproduction Problems\u2019, Epidemiology and Microbiology Institute, 3, K. 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