{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:46:52Z","timestamp":1785415612126,"version":"3.56.0"},"reference-count":82,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brasil","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"https:\/\/ror.org\/00x0ma614","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>A\u00e7a\u00ed is considered a super fruit for its high concentration of flavonoid polyphenols, predominantly anthocyanins. The nutraceutical effect of a\u00e7a\u00ed as an anti-inflammatory and antiapoptotic agent has been reported. This study aimed to investigate whether a\u00e7a\u00ed protects paternal contribution against accelerated aging using the D-galactose model. Wistar rats (n = 24) were treated by voluntary consumption with vehicle paste (C) or a mixture with 200 mg\/kg of D-galactose (DG) or 300 mg\/kg of lyophilized a\u00e7a\u00ed (A); D-galactose and a\u00e7a\u00ed (DGA) were given separately. Fathers (F0) and offspring (F1) underwent Doppler ultrasound (aorta and kidney) and spermatic and immune cell analyses. In the F0 generation, the DG group showed altered epididymis weight, sperm quality, aortic diameter, neutrophil-to-lymphocyte ratio, systolic velocity and renal resistivity index. A\u00e7a\u00ed positively modulated sperm, neutrophil and vascular alterations in F0. The male F1 of the DG group showed lower body mass, as well as a greater number of abnormal spermatozoa and leukocyte DNA breaks; however, the DGA group had a significant increase in body mass and the NRL was positively correlated with leukocyte DNA breaks. Our data indicate that age acceleration, induced by D-galactose for 30 days, causes innate physiological dysfunction and compromises sperm contribution, which was positively modulated by a\u00e7a\u00ed. The preconception use of lyophilized a\u00e7a\u00ed also has some positive impact on intergenerational health, but the topic still deserves further investigation.<\/jats:p>","DOI":"10.3390\/biology15131086","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T14:01:08Z","timestamp":1783346468000},"page":"1086","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sperm, Neutrophil and Vascular Alterations in Advanced Paternal Age Model and the Nutraceutical Effect of A\u00e7a\u00ed to Mitigate Health Vulnerability in the Male Offspring"],"prefix":"10.3390","volume":"15","author":[{"given":"Amanda Guimar\u00e3es","family":"de Araujo","sequence":"first","affiliation":[{"name":"Laboratory of Reproductive and Developmental Biology (LaBReD), Department of Morphology and Genetics, Paulista School of Medicine, Federal University of Sao Paulo-EPM\/UNIFESP, Sao Paulo 04023-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eder Henrique Alves","family":"Pinto","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive and Developmental Biology (LaBReD), Department of Morphology and Genetics, Paulista School of Medicine, Federal University of Sao Paulo-EPM\/UNIFESP, Sao Paulo 04023-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3580-982X","authenticated-orcid":false,"given":"Jo\u00e3o Carlos Ara\u00fajo","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive and Developmental Biology (LaBReD), Department of Morphology and Genetics, Paulista School of Medicine, Federal University of Sao Paulo-EPM\/UNIFESP, Sao Paulo 04023-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz Guerra","family":"Pompermayer","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive and Developmental Biology (LaBReD), Department of Morphology and Genetics, Paulista School of Medicine, Federal University of Sao Paulo-EPM\/UNIFESP, Sao Paulo 04023-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephany","family":"de Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive and Developmental Biology (LaBReD), Department of Morphology and Genetics, Paulista School of Medicine, Federal University of Sao Paulo-EPM\/UNIFESP, Sao Paulo 04023-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ria","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive and Developmental Biology (LaBReD), Department of Morphology and Genetics, Paulista School of Medicine, Federal University of Sao Paulo-EPM\/UNIFESP, Sao Paulo 04023-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4342-6688","authenticated-orcid":false,"given":"M\u00f4nica Marques","family":"Telles","sequence":"additional","affiliation":[{"name":"Department of Physiology, Discipline of Nutrition, Paulista School of Medicine, Federal University of Sao Paulo-EPM\/UNIFESP, Sao Paulo 04021-001, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7217-4195","authenticated-orcid":false,"given":"Vanessa","family":"Vendramini","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive and Developmental Biology (LaBReD), Department of Morphology and Genetics, Paulista School of Medicine, Federal University of Sao Paulo-EPM\/UNIFESP, Sao Paulo 04023-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1080\/00324728.2023.2298678","article-title":"Change in the perceived reproductive age window and delayed fertility in Europe","volume":"79","author":"LazzariP","year":"2025","journal-title":"Popul. 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