{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:19:34Z","timestamp":1785410374850,"version":"3.56.0"},"reference-count":157,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007287","name":"Worldwide Cancer Research","doi-asserted-by":"publisher","award":["25-0103"],"award-info":[{"award-number":["25-0103"]}],"id":[{"id":"10.13039\/501100007287","id-type":"DOI","asserted-by":"publisher"}]},{"award":["25-0103"],"award-info":[{"award-number":["25-0103"]}],"id":[{"id":"https:\/\/ror.org\/031tfbz57","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Fondation Sant\u00e9"},{"id":[{"id":"https:\/\/ror.org\/03h0c3p34","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>During mitosis sister chromatids are accurately segregated into two daughter cells through a microtubule-based structure known as the mitotic spindle. Errors in mitotic spindle formation have been associated with human diseases and tumorigenesis. Chk1 has well established roles in DNA damage response and replication checkpoints. Recently, new roles have emerged in the onset of mitosis, chromosome segregation and cytokinesis. In this review, we recapitulate the known roles of Chk1 in cell division and highlight new roles of Chk1 in mitotic spindle assembly and its contribution to genome stability. Specifically, it was recently reported that, during the first stages of mitosis, ATRIP and its interacting partners, ATR and TopBP1, are recruited to centrosomes, where they activate Chk1. Then, Chk1 phosphorylates \u03b2-tubulin at Threonine 285 (T285), promoting microtubule nucleation and mitotic spindle formation. \u03b2-tubulin phosphorylation by Chk1 is required for proper mitotic progression, cytokinesis with equal-sized daughter cells and cell proliferation. This novel ATR-Chk1 signaling pathway reinforces the crosstalk between the cell cycle regulation and DNA damage response.<\/jats:p>","DOI":"10.3390\/biology15141105","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T08:50:56Z","timestamp":1783587056000},"page":"1105","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["New Roles of Chk1 in Spindle Formation and Genome Integrity"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2066-2872","authenticated-orcid":false,"given":"Sofia","family":"Balafouti","sequence":"first","affiliation":[{"name":"Department of Biology, University of Crete, Vassilika Vouton, 70013 Heraklion, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Zachos","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Crete, Vassilika Vouton, 70013 Heraklion, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7693-3531","authenticated-orcid":false,"given":"Eleni","family":"Petsalaki","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Crete, Vassilika Vouton, 70013 Heraklion, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1038\/nrm.2016.162","article-title":"Mitotic spindle assembly in animal cells: A fine balancing act","volume":"18","author":"Prosser","year":"2017","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1146\/annurev-biochem-060815-014528","article-title":"Mechanisms of Mitotic Spindle Assembly","volume":"85","author":"Petry","year":"2016","journal-title":"Annu. Rev. Biochem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0074-7696(07)65003-7","article-title":"Mechanisms of mitotic spindle assembly and function","volume":"265","author":"Walczak","year":"2008","journal-title":"Int. Rev. Cytol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1038\/nrm3010","article-title":"Asymmetric cell division: Recent developments and their implications for tumour biology","volume":"11","author":"Knoblich","year":"2010","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/1747-1028-7-19","article-title":"Transient defects of mitotic spindle geometry and chromosome segregation errors","volume":"7","author":"Silkworth","year":"2012","journal-title":"Cell Div."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1083\/jcb.201209015","article-title":"Mitotic spindle (DIS)orientation and DISease: Cause or consequence?","volume":"199","author":"Noatynska","year":"2012","journal-title":"J. Cell Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.molcel.2004.07.012","article-title":"Mitotic spindle assembly and chromosome segregation: Refocusing on microtubule dynamics","volume":"15","author":"Walczak","year":"2004","journal-title":"Mol. Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/34465","article-title":"Structure of the alpha beta tubulin dimer by electron crystallography","volume":"391","author":"Nogales","year":"1998","journal-title":"Nature"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Wloga, D., Joachimiak, E., and Fabczak, H. (2017). Tubulin Post-Translational Modifications and Microtubule Dynamics. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18102207"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"101511","DOI":"10.1016\/j.isci.2020.101511","article-title":"The Mechanism of Tubulin Assembly into Microtubules: Insights from Structural Studies","volume":"23","author":"Knossow","year":"2020","journal-title":"iScience"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.ceb.2009.11.008","article-title":"From signaling pathways to microtubule dynamics: The key players","volume":"22","year":"2010","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1093\/jb\/mvg148","article-title":"\u201cSearch-and-capture\u201d of microtubules through plus-end-binding proteins (+TIPs)","volume":"134","author":"Tsukita","year":"2003","journal-title":"J. Biochem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.cell.2011.07.012","article-title":"The spatial arrangement of chromosomes during prometaphase facilitates spindle assembly","volume":"146","author":"Magidson","year":"2011","journal-title":"Cell"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/j.cub.2005.03.019","article-title":"Efficient chromosome capture requires a bias in the \u2018search-and-capture\u2019 process during mitotic-spindle assembly","volume":"15","author":"Wollman","year":"2005","journal-title":"Curr. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s00249-017-1244-4","article-title":"Mitotic spindle: Kinetochore fibers hold on tight to interpolar bundles","volume":"47","author":"Tolic","year":"2018","journal-title":"Eur. Biophys. J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e105432","DOI":"10.15252\/embj.2020105432","article-title":"Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins","volume":"39","author":"Steblyanko","year":"2020","journal-title":"EMBO J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S0092-8674(00)00048-9","article-title":"Xkid, a chromokinesin required for chromosome alignment on the metaphase plate","volume":"102","author":"Antonio","year":"2000","journal-title":"Cell"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/B978-0-12-407697-6.00006-4","article-title":"New insights into the mechanism for chromosome alignment in metaphase","volume":"303","author":"Guo","year":"2013","journal-title":"Int. Rev. Cell Mol. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1038\/nature02256","article-title":"Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase","volume":"427","author":"Rogers","year":"2004","journal-title":"Nature"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Bunning, A.R., and Gupta, M.L. (2023). The importance of microtubule-dependent tension in accurate chromosome segregation. Front. Cell Dev. Biol., 11.","DOI":"10.3389\/fcell.2023.1096333"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1083\/jcb.141.3.703","article-title":"Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindles","volume":"141","author":"Desai","year":"1998","journal-title":"J. Cell Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1016\/j.cell.2007.11.011","article-title":"Cytokinesis: Placing and making the final cut","volume":"131","author":"Barr","year":"2007","journal-title":"Cell"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s00412-011-0330-0","article-title":"The ABCs of CENPs","volume":"120","author":"Perpelescu","year":"2011","journal-title":"Chromosoma"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/j.cell.2006.09.039","article-title":"The conserved KMN network constitutes the core microtubule-binding site of the kinetochore","volume":"127","author":"Cheeseman","year":"2006","journal-title":"Cell"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Krenn, V., and Musacchio, A. (2015). The Aurora B Kinase in Chromosome Bi-Orientation and Spindle Checkpoint Signaling. Front. Oncol., 5.","DOI":"10.3389\/fonc.2015.00225"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e02137","DOI":"10.7554\/eLife.02137","article-title":"The quantitative architecture of centromeric chromatin","volume":"3","author":"Bodor","year":"2014","journal-title":"eLife"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"10084","DOI":"10.1074\/jbc.RA118.003154","article-title":"The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser\/Thr kinases","volume":"293","author":"Ciossani","year":"2018","journal-title":"J. Biol. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2229","DOI":"10.1128\/MCB.22.7.2229-2241.2002","article-title":"CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells","volume":"22","author":"Ando","year":"2002","journal-title":"Mol. Cell Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/j.cell.2008.10.019","article-title":"CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore","volume":"135","author":"Hori","year":"2008","journal-title":"Cell"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.1091\/mbc.e07-01-0045","article-title":"CENP-C is involved in chromosome segregation, mitotic checkpoint function, and kinetochore assembly","volume":"18","author":"Kwon","year":"2007","journal-title":"Mol. Biol. Cell"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.cub.2011.02.005","article-title":"CENP-C is a structural platform for kinetochore assembly","volume":"21","author":"Przewloka","year":"2011","journal-title":"Curr. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.cub.2010.12.039","article-title":"Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore","volume":"21","author":"Screpanti","year":"2011","journal-title":"Curr. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nrm3916","article-title":"Regulation of kinetochore-microtubule attachments through homeostatic control during mitosis","volume":"16","author":"Godek","year":"2015","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1016\/j.cub.2019.07.052","article-title":"Centromere Dysfunction Compromises Mitotic Spindle Pole Integrity","volume":"29","author":"Gemble","year":"2019","journal-title":"Curr. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.semcdb.2021.06.009","article-title":"Spindle assembly checkpoint activation and silencing at kinetochores","volume":"117","author":"Pines","year":"2021","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/s41580-023-00593-z","article-title":"Principles and dynamics of spindle assembly checkpoint signalling","volume":"24","author":"McAinsh","year":"2023","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.tcb.2018.09.007","article-title":"Posing the APC\/C E3 Ubiquitin Ligase to Orchestrate Cell Division","volume":"29","author":"Watson","year":"2019","journal-title":"Trends Cell Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.sbi.2019.11.010","article-title":"Structural interconversions of the anaphase-promoting complex\/cyclosome (APC\/C) regulate cell cycle transitions","volume":"61","author":"Barford","year":"2020","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"R1002","DOI":"10.1016\/j.cub.2015.08.051","article-title":"The Molecular Biology of Spindle Assembly Checkpoint Signaling Dynamics","volume":"25","author":"Musacchio","year":"2015","journal-title":"Curr. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1083\/jcb.200102093","article-title":"Checkpoint inhibition of the APC\/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2","volume":"154","author":"Sudakin","year":"2001","journal-title":"J. Cell Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"180109","DOI":"10.1098\/rsob.180109","article-title":"Leader of the SAC: Molecular mechanisms of Mps1\/TTK regulation in mitosis","volume":"8","author":"Pachis","year":"2018","journal-title":"Open Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1083\/jcb.201311015","article-title":"A Bub1-Mad1 interaction targets the Mad1-Mad2 complex to unattached kinetochores to initiate the spindle checkpoint","volume":"204","author":"Moyle","year":"2014","journal-title":"J. Cell Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1083\/jcb.201002133","article-title":"Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex","volume":"190","author":"Hewitt","year":"2010","journal-title":"J. Cell Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2915","DOI":"10.1016\/j.cub.2017.08.033","article-title":"BubR1 Promotes Bub3-Dependent APC\/C Inhibition during Spindle Assembly Checkpoint Signaling","volume":"27","author":"Overlack","year":"2017","journal-title":"Curr. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1038\/nature10896","article-title":"Structure of the mitotic checkpoint complex","volume":"484","author":"Chao","year":"2012","journal-title":"Nature"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1083\/jcb.200111001","article-title":"Human securin proteolysis is controlled by the spindle checkpoint and reveals when the APC\/C switches from activation by Cdc20 to Cdh1","volume":"157","author":"Hagting","year":"2002","journal-title":"J. Cell Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1038\/ncb1066","article-title":"Securin and B-cyclin\/CDK are the only essential targets of the APC","volume":"5","author":"Thornton","year":"2003","journal-title":"Nat. Cell Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1038\/nature13911","article-title":"The mitotic checkpoint complex binds a second CDC20 to inhibit active APC\/C","volume":"517","author":"Izawa","year":"2015","journal-title":"Nature"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/nrm3494","article-title":"Microtubule attachment and spindle assembly checkpoint signalling at the kinetochore","volume":"14","author":"Foley","year":"2013","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1038\/s41392-024-01767-7","article-title":"The two sides of chromosomal instability: Drivers and brakes in cancer","volume":"9","author":"Hosea","year":"2024","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1016\/S0960-9822(95)00276-4","article-title":"The core of the mammalian centriole contains gamma-tubulin","volume":"5","author":"Fuller","year":"1995","journal-title":"Curr. Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1083\/jcb.146.3.585","article-title":"The sudden recruitment of gamma-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules","volume":"146","author":"Khodjakov","year":"1999","journal-title":"J. Cell Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1038\/nrm3209","article-title":"Microtubule nucleation by gamma-tubulin complexes","volume":"12","author":"Kollman","year":"2011","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Haren, L., Stearns, T., and Luders, J. (2009). Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0005976"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Magnaghi-Jaulin, L., Eot-Houllier, G., Gallaud, E., and Giet, R. (2019). Aurora A Protein Kinase: To the Centrosome and Beyond. Biomolecules, 9.","DOI":"10.3390\/biom9010028"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1700","DOI":"10.1111\/febs.15240","article-title":"DNA damage response proteins regulating mitotic cell division: Double agents preserving genome stability","volume":"287","author":"Petsalaki","year":"2020","journal-title":"FEBS J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/s41392-018-0021-x","article-title":"gamma-tubulin as a signal-transducing molecule and meshwork with therapeutic potential","volume":"3","year":"2018","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_58","first-page":"1183","article-title":"Overexpression of gamma-tubulin in non-small cell lung cancer","volume":"27","author":"Maounis","year":"2012","journal-title":"Histol. Histopathol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1111\/j.1349-7006.2008.01075.x","article-title":"Increased expression of centrosomal alpha, gamma-tubulin in atypical ductal hyperplasia and carcinoma of the breast","volume":"100","author":"Niu","year":"2009","journal-title":"Cancer Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1002\/cm.21559","article-title":"Mapping the kinetochore MAP functions required for stabilizing microtubule attachments to chromosomes during metaphase","volume":"76","author":"Amin","year":"2019","journal-title":"Cytoskeleton"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.tcb.2015.08.009","article-title":"Regulation of Microtubule Growth and Catastrophe: Unifying Theory and Experiment","volume":"25","author":"Hibbel","year":"2015","journal-title":"Trends Cell Biol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"4155","DOI":"10.1083\/jcb.201711182","article-title":"Structural insight into microtubule stabilization and kinesin inhibition by Tau family MAPs","volume":"217","author":"Shigematsu","year":"2018","journal-title":"J. Cell Biol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3447","DOI":"10.1242\/jcs.063727","article-title":"Post-translational modifications of microtubules","volume":"123","author":"Wloga","year":"2010","journal-title":"J. Cell Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.tcb.2014.10.004","article-title":"Post-translational modifications of tubulin: Pathways to functional diversity of microtubules","volume":"25","author":"Song","year":"2015","journal-title":"Trends Cell Biol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"R351","DOI":"10.1016\/j.cub.2014.03.032","article-title":"Post-translational modifications of tubulin","volume":"24","author":"Magiera","year":"2014","journal-title":"Curr. Biol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1038\/nrm1260","article-title":"Post-translational modifications regulate microtubule function","volume":"4","author":"Westermann","year":"2003","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1091\/mbc.e05-07-0621","article-title":"Microtubule regulation in mitosis: Tubulin phosphorylation by the cyclin-dependent kinase Cdk1","volume":"17","author":"Peris","year":"2006","journal-title":"Mol. Biol. Cell"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1101\/gad.314351.118","article-title":"The impact of mitotic errors on cell proliferation and tumorigenesis","volume":"32","author":"Levine","year":"2018","journal-title":"Genes. Dev."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1038\/ncb2897","article-title":"Causes and consequences of replication stress","volume":"16","author":"Zeman","year":"2014","journal-title":"Nat. Cell Biol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"11626","DOI":"10.1093\/nar\/gkae584","article-title":"Partial wrapping of single-stranded DNA by replication protein A and modulation through phosphorylation","volume":"52","author":"Chadda","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1002\/bies.201400107","article-title":"Replication protein A: Single-stranded DNA\u2019s first responder: Dynamic DNA-interactions allow replication protein A to direct single-strand DNA intermediates into different pathways for synthesis or repair","volume":"36","author":"Chen","year":"2014","journal-title":"Bioessays"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1073\/pnas.0510223103","article-title":"ATRIP associates with replication protein A-coated ssDNA through multiple interactions","volume":"103","author":"Namiki","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"8279","DOI":"10.1093\/nar\/gkac588","article-title":"Structure of the human RAD17-RFC clamp loader and 9-1-1 checkpoint clamp bound to a dsDNA-ssDNA junction","volume":"50","author":"Day","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1101\/gad.1547007","article-title":"The Rad9-Hus1-Rad1 (9-1-1) clamp activates checkpoint signaling via TopBP1","volume":"21","author":"Delacroix","year":"2007","journal-title":"Genes. Dev."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1093\/nar\/gkq743","article-title":"Structure and function of the Rad9-binding region of the DNA-damage checkpoint adaptor TopBP1","volume":"39","author":"Rappas","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1101\/gad.1666208","article-title":"TopBP1 activates ATR through ATRIP and a PIKK regulatory domain","volume":"22","author":"Mordes","year":"2008","journal-title":"Genes. Dev."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/j.cell.2005.12.041","article-title":"TopBP1 activates the ATR-ATRIP complex","volume":"124","author":"Kumagai","year":"2006","journal-title":"Cell"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"3257","DOI":"10.1016\/j.cub.2016.10.030","article-title":"RPA-Binding Protein ETAA1 Is an ATR Activator Involved in DNA Replication Stress Response","volume":"26","author":"Lee","year":"2016","journal-title":"Curr. Biol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"4129","DOI":"10.1128\/MCB.21.13.4129-4139.2001","article-title":"ATR-mediated checkpoint pathways regulate phosphorylation and activation of human Chk1","volume":"21","author":"Zhao","year":"2001","journal-title":"Mol. Cell Biol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1101\/gad.14.12.1448","article-title":"Chk1 is an essential kinase that is regulated by Atr and required for the G(2)\/M DNA damage checkpoint","volume":"14","author":"Liu","year":"2000","journal-title":"Genes. Dev."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"21767","DOI":"10.1074\/jbc.M300229200","article-title":"Chk1 mediates S and G2 arrests through Cdc25A degradation in response to DNA-damaging agents","volume":"278","author":"Xiao","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Saldanha, J., Rageul, J., Patel, J.A., and Kim, H. (2023). The Adaptive Mechanisms and Checkpoint Responses to a Stressed DNA Replication Fork. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241310488"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Gorecki, L., Andrs, M., and Korabecny, J. (2021). Clinical Candidates Targeting the ATR-CHK1-WEE1 Axis in Cancer. Cancers, 13.","DOI":"10.3390\/cancers13040795"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.molcel.2013.03.006","article-title":"A role for the MRN complex in ATR activation via TOPBP1 recruitment","volume":"50","author":"Duursma","year":"2013","journal-title":"Mol. Cell"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1016\/j.celrep.2013.04.018","article-title":"Two distinct modes of ATR activation orchestrated by Rad17 and Nbs1","volume":"3","author":"Shiotani","year":"2013","journal-title":"Cell Rep."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1038\/ncb1165","article-title":"Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase","volume":"6","author":"Kramer","year":"2004","journal-title":"Nat. Cell Biol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"34223","DOI":"10.1074\/jbc.C109.051540","article-title":"Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during G2\/M transition","volume":"284","author":"Enomoto","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"4269","DOI":"10.1242\/jcs.03200","article-title":"CHK1 phosphorylates CDC25B during the cell cycle in the absence of DNA damage","volume":"119","author":"Schmitt","year":"2006","journal-title":"J. Cell Sci."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.4161\/cc.4.9.1964","article-title":"CDC25B phosphorylation by Aurora-A occurs at the G2\/M transition and is inhibited by DNA damage","volume":"4","author":"Cazales","year":"2005","journal-title":"Cell Cycle"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.1242\/jcs.01108","article-title":"Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2-M transition","volume":"117","author":"Dutertre","year":"2004","journal-title":"J. Cell Sci."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"11964","DOI":"10.1073\/pnas.0604987103","article-title":"Checkpoint kinase 1 (Chk1) is required for mitotic progression through negative regulation of polo-like kinase 1 (Plk1)","volume":"103","author":"Tang","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.devcel.2007.01.003","article-title":"Chk1 is required for spindle checkpoint function","volume":"12","author":"Zachos","year":"2007","journal-title":"Dev. Cell"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1083\/jcb.201104023","article-title":"Phosphorylation at serine 331 is required for Aurora B activation","volume":"195","author":"Petsalaki","year":"2011","journal-title":"J. Cell Biol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1126\/science.aan6490","article-title":"A mitosis-specific and R loop-driven ATR pathway promotes faithful chromosome segregation","volume":"359","author":"Kabeche","year":"2018","journal-title":"Science"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1016\/j.ceb.2009.09.008","article-title":"Making the Auroras glow: Regulation of Aurora A and B kinase function by interacting proteins","volume":"21","author":"Carmena","year":"2009","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/nrm3474","article-title":"The chromosomal passenger complex (CPC): From easy rider to the godfather of mitosis","volume":"13","author":"Carmena","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"3325","DOI":"10.1091\/mbc.e02-11-0769","article-title":"Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis","volume":"14","author":"Honda","year":"2003","journal-title":"Mol. Biol. Cell"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Kitagawa, M., and Lee, S.H. (2015). The chromosomal passenger complex (CPC) as a key orchestrator of orderly mitotic exit and cytokinesis. Front. Cell Dev. Biol., 3.","DOI":"10.3389\/fcell.2015.00014"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"27577","DOI":"10.1074\/jbc.C200307200","article-title":"Phosphorylation of the carboxyl terminus of inner centromere protein (INCENP) by the Aurora B Kinase stimulates Aurora B kinase activity","volume":"277","author":"Bishop","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.molcel.2005.03.031","article-title":"Mechanism of Aurora B activation by INCENP and inhibition by hesperadin","volume":"18","author":"Sessa","year":"2005","journal-title":"Mol. Cell"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"120136","DOI":"10.1098\/rsob.120136","article-title":"On the molecular mechanisms of mitotic kinase activation","volume":"2","author":"Bayliss","year":"2012","journal-title":"Open Biol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.tcb.2021.09.008","article-title":"Changing places: Chromosomal Passenger Complex relocation in early anaphase","volume":"32","author":"Hadders","year":"2022","journal-title":"Trends Cell Biol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"15542","DOI":"10.1038\/ncomms15542","article-title":"Inner centromere localization of the CPC maintains centromere cohesion and allows mitotic checkpoint silencing","volume":"8","author":"Hengeveld","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"102462","DOI":"10.1016\/j.ceb.2024.102462","article-title":"Organization of the chromosomal passenger complex clusters at inner centromeres in mitosis","volume":"92","author":"Matsui","year":"2025","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"e202008029","DOI":"10.1083\/jcb.202008029","article-title":"An ATM-Chk2-INCENP pathway activates the abscission checkpoint","volume":"220","author":"Petsalaki","year":"2021","journal-title":"J. Cell Biol."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1038\/ncomms1319","article-title":"Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis","volume":"2","author":"Saurin","year":"2011","journal-title":"Nat. Commun."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"116019","DOI":"10.1016\/j.celrep.2025.116019","article-title":"The mitotic ATR-Chk1 pathway promotes CDK1 activity for faithful chromosome segregation","volume":"44","author":"Joo","year":"2025","journal-title":"Cell Rep."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1038\/ncb1350","article-title":"Complex formation of Plk1 and INCENP required for metaphase-anaphase transition","volume":"8","author":"Goto","year":"2006","journal-title":"Nat. Cell Biol."},{"key":"ref_109","first-page":"32","article-title":"Merotelic kinetochore orientation, aneuploidy, and cancer","volume":"1786","author":"Cimini","year":"2008","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1007\/s00018-012-1057-7","article-title":"Regulatory mechanisms of kinetochore-microtubule interaction in mitosis","volume":"70","author":"Tanaka","year":"2013","journal-title":"Cell Mol. Life Sci."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1016\/j.cub.2006.07.057","article-title":"Aurora B is enriched at merotelic attachment sites, where it regulates MCAK","volume":"16","author":"Knowlton","year":"2006","journal-title":"Curr. Biol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1083\/jcb.200208092","article-title":"The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint","volume":"161","author":"Hauf","year":"2003","journal-title":"J. Cell Biol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.tcb.2011.01.003","article-title":"Merotelic kinetochore attachment: Causes and effects","volume":"21","author":"Gregan","year":"2011","journal-title":"Trends Cell Biol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1242\/jcs.119677","article-title":"Chk1 and Mps1 jointly regulate correction of merotelic kinetochore attachments","volume":"126","author":"Petsalaki","year":"2013","journal-title":"J. Cell Sci."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.cell.2007.11.046","article-title":"Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment","volume":"132","author":"Jelluma","year":"2008","journal-title":"Cell"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.molcel.2010.02.034","article-title":"Aurora B phosphorylates spatially distinct targets to differentially regulate the kinetochore-microtubule interface","volume":"38","author":"Welburn","year":"2010","journal-title":"Mol. Cell"},{"key":"ref_117","doi-asserted-by":"crossref","unstructured":"McVey, S.L., Cosby, J.K., and Nannas, N.J. (2021). Aurora B Tension Sensing Mechanisms in the Kinetochore Ensure Accurate Chromosome Segregation. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22168818"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"e202007030","DOI":"10.1083\/jcb.202007030","article-title":"Tension promotes kinetochore-microtubule release by Aurora B kinase","volume":"220","author":"Chen","year":"2021","journal-title":"J. Cell Biol."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1083\/jcb.201412139","article-title":"Unattached kinetochores rather than intrakinetochore tension arrest mitosis in taxol-treated cells","volume":"212","author":"Magidson","year":"2016","journal-title":"J. Cell Biol."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"10036","DOI":"10.1038\/ncomms10036","article-title":"Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells","volume":"6","author":"Tauchman","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"8987","DOI":"10.1038\/ncomms9987","article-title":"Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint","volume":"6","author":"Etemad","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.4161\/cc.8.15.9169","article-title":"Chking and executing cell division to prevent genomic instability","volume":"8","author":"Peddibhotla","year":"2009","journal-title":"Cell Cycle"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"5159","DOI":"10.1073\/pnas.0806671106","article-title":"The DNA-damage effector checkpoint kinase 1 is essential for chromosome segregation and cytokinesis","volume":"106","author":"Peddibhotla","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"19548","DOI":"10.1074\/jbc.M117.811646","article-title":"Checkpoint kinase 1-induced phosphorylation of O-linked beta-N-acetylglucosamine transferase regulates the intermediate filament network during cytokinesis","volume":"292","author":"Li","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_125","doi-asserted-by":"crossref","unstructured":"Finardi, A., Massari, L.F., and Visintin, R. (2020). Anaphase Bridges: Not All Natural Fibers Are Healthy. Genes, 11.","DOI":"10.3390\/genes11080902"},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s00412-004-0284-6","article-title":"Resolution of anaphase bridges in cancer cells","volume":"112","author":"Hoffelder","year":"2004","journal-title":"Chromosoma"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1083\/jcb.201210040","article-title":"Linking abnormal mitosis to the acquisition of DNA damage","volume":"199","author":"Ganem","year":"2012","journal-title":"J. Cell Biol."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.cell.2008.12.020","article-title":"Aurora B-mediated abscission checkpoint protects against tetraploidization","volume":"136","author":"Steigemann","year":"2009","journal-title":"Cell"},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.tcb.2016.10.001","article-title":"The Abscission Checkpoint: Making It to the Final Cut","volume":"27","author":"Nahse","year":"2017","journal-title":"Trends Cell Biol."},{"key":"ref_130","first-page":"3902","article-title":"Chk1 protects against chromatin bridges by constitutively phosphorylating BLM serine 502 to inhibit BLM degradation","volume":"127","author":"Petsalaki","year":"2014","journal-title":"J. Cell Sci."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"3071","DOI":"10.1083\/jcb.201802102","article-title":"Src activation by Chk1 promotes actin patch formation and prevents chromatin bridge breakage in cytokinesis","volume":"217","author":"Dandoulaki","year":"2018","journal-title":"J. Cell Biol."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"5834","DOI":"10.1038\/s44318-025-00565-3","article-title":"Tension-sensitive LINC-RhoA signaling prevents chromatin bridge breakage in cytokinesis","volume":"44","author":"Balafouti","year":"2025","journal-title":"EMBO J."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1038\/s42003-026-09862-x","article-title":"beta-tubulin phosphorylation by Chk1 is required for normal spindle formation during cell division","volume":"9","author":"Boutakoglou","year":"2026","journal-title":"Commun. Biol."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"150160","DOI":"10.1098\/rsob.150160","article-title":"The RZZ complex requires the N-terminus of KNL1 to mediate optimal Mad1 kinetochore localization in human cells","volume":"5","author":"Caldas","year":"2015","journal-title":"Open Biol."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.cub.2005.03.052","article-title":"Recruitment of Mad2 to the kinetochore requires the Rod\/Zw10 complex","volume":"15","author":"Buffin","year":"2005","journal-title":"Curr. Biol."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"2372","DOI":"10.1091\/mbc.e04-11-1006","article-title":"ATRIP binding to replication protein A-single-stranded DNA promotes ATR-ATRIP localization but is dispensable for Chk1 phosphorylation","volume":"16","author":"Ball","year":"2005","journal-title":"Mol. Biol. Cell"},{"key":"ref_137","doi-asserted-by":"crossref","unstructured":"Joo, Y.K., Ramirez, C., and Kabeche, L. (2024). A TRilogy of ATR\u2019s Non-Canonical Roles Throughout the Cell Cycle and Its Relation to Cancer. Cancers, 16.","DOI":"10.3390\/cancers16203536"},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1073\/pnas.1217781110","article-title":"Nek1 kinase associates with ATR-ATRIP and primes ATR for efficient DNA damage signaling","volume":"110","author":"Liu","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/nrm4025","article-title":"Short- and long-term effects of chromosome mis-segregation and aneuploidy","volume":"16","author":"Santaguida","year":"2015","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"2014","DOI":"10.1038\/s41588-024-01916-2","article-title":"Aneuploidy as a driver of human cancer","volume":"56","author":"Sdeor","year":"2024","journal-title":"Nat. Genet."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.ceb.2005.11.004","article-title":"Cytoskeletal genes regulating brain size","volume":"18","author":"Bond","year":"2006","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1242\/jcs.081406","article-title":"Mitotic spindle misorientation in cancer--out of alignment and into the fire","volume":"124","author":"Pease","year":"2011","journal-title":"J. Cell Sci."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0092-8674(00)80961-7","article-title":"High-resolution model of the microtubule","volume":"96","author":"Nogales","year":"1999","journal-title":"Cell"},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1006\/jmbi.2001.5077","article-title":"Refined structure of alpha beta-tubulin at 3.5 A resolution","volume":"313","author":"Lowe","year":"2001","journal-title":"J. Mol. Biol."},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1016\/j.str.2010.05.010","article-title":"Structural basis of interprotofilament interaction and lateral deformation of microtubules","volume":"18","author":"Sui","year":"2010","journal-title":"Structure"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1042\/BST20240360","article-title":"Structural switching of tubulin in the microtubule lattice","volume":"53","author":"Chew","year":"2025","journal-title":"Biochem. Soc. Trans."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"5980","DOI":"10.1038\/s41467-023-41615-w","article-title":"Structural insights into the mechanism of GTP initiation of microtubule assembly","volume":"14","author":"Zhou","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_148","doi-asserted-by":"crossref","unstructured":"Sulimenko, V., Draberova, E., and Draber, P. (2022). gamma-Tubulin in microtubule nucleation and beyond. Front. Cell Dev. Biol., 10.","DOI":"10.3389\/fcell.2022.880761"},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1038\/nrd3253","article-title":"Microtubule-binding agents: A dynamic field of cancer therapeutics","volume":"9","author":"Dumontet","year":"2010","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_150","doi-asserted-by":"crossref","unstructured":"Assuncao, H.C., Silva, P.M.A., Bousbaa, H., and Cidade, H. (2025). Recent Advances in Microtubule Targeting Agents for Cancer Therapy. Molecules, 30.","DOI":"10.3390\/molecules30163314"},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s12929-014-0107-x","article-title":"KUD773, a phenylthiazole derivative, displays anticancer activity in human hormone-refractory prostate cancers through inhibition of tubulin polymerization and anti-Aurora A activity","volume":"22","author":"Yu","year":"2015","journal-title":"J. Biomed. Sci."},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.exphem.2017.06.003","article-title":"A novel dual inhibitor of microtubule and Bruton\u2019s tyrosine kinase inhibits survival of multiple myeloma and osteoclastogenesis","volume":"53","author":"Pandey","year":"2017","journal-title":"Exp. Hematol."},{"key":"ref_153","doi-asserted-by":"crossref","unstructured":"Lafanechere, L. (2022). The microtubule cytoskeleton: An old validated target for novel therapeutic drugs. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.969183"},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1038\/s41568-022-00535-5","article-title":"Targeting DNA damage response pathways in cancer","volume":"23","author":"Groelly","year":"2023","journal-title":"Nat. Rev. Cancer"},{"key":"ref_155","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.3390\/ph3051311","article-title":"Prospects for the Use of ATR Inhibitors to Treat Cancer","volume":"3","author":"Wagner","year":"2010","journal-title":"Pharmaceuticals"},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s13045-020-00874-6","article-title":"Participation of the ATR\/CHK1 pathway in replicative stress targeted therapy of high-grade ovarian cancer","volume":"13","author":"Gralewska","year":"2020","journal-title":"J. Hematol. Oncol."},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.canlet.2020.09.016","article-title":"Reality CHEK: Understanding the biology and clinical potential of CHK1","volume":"497","author":"Bhattacharya","year":"2021","journal-title":"Cancer Lett."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/14\/1105\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T09:17:40Z","timestamp":1783588660000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/14\/1105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,9]]},"references-count":157,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["biology15141105"],"URL":"https:\/\/doi.org\/10.3390\/biology15141105","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,9]]}}}