{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:09:33Z","timestamp":1791158973719,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004944","name":"Research budget income fund, Faculty of Science, Naresuan University","doi-asserted-by":"publisher","award":["R2567E_SCI014"],"award-info":[{"award-number":["R2567E_SCI014"]}],"id":[{"id":"10.13039\/501100004944","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Bacopa monnieri (Brahmi), a valuable medicinal herb, faces challenges in consistent production and quality under natural cultivation. To overcome these limitations, this study investigated the impact of different light qualities (warm-white, red, blue, and green) on the in vitro growth, chlorophyll content, and accumulation of bacosides in B. monnieri using a temporary immersion system (TIS). The results revealed that green and blue light significantly enhanced biomass accumulation, with green light promoting the highest number of new shoots (24.2 shoots\/clump) and blue light inducing the development of longer shoots with extended internodes (2.6 cm\/internode). Interestingly, while green light resulted in the lowest chlorophyll content, it led to the highest accumulation of bacoside A3 (0.39% DW). In contrast, no significant differences were observed in bacopaside II content across all light treatments. However, bacopasaponin C production was significantly enhanced by warm-white and red light. These results demonstrate the potential of manipulating light quality within a TIS to optimize specific growth parameters and bioactive compound production in B. monnieri, offering valuable insights for enhancing the quality and yield of this important medicinal plant for industrial applications.<\/jats:p>","DOI":"10.3390\/biology15141116","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T09:46:47Z","timestamp":1783676807000},"page":"1116","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Monochromatic Light on Mass and Phytocompound Production of Bacopa monnieri (L.) Wettst. in the Temporary Immersion System"],"prefix":"10.3390","volume":"15","author":[{"given":"Teerawech","family":"Promchiangsa","sequence":"first","affiliation":[{"name":"Division of Basic and Medical Sciences, Faculty of Allied Heal Sciences, Pathumthani University, Pathum Thani 12000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phatthanan","family":"Khiaokhoen","sequence":"additional","affiliation":[{"name":"Plant Tissue Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anupan","family":"Kongbangkerd","sequence":"additional","affiliation":[{"name":"Plant Tissue Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8360-1809","authenticated-orcid":false,"given":"Boworn","family":"Kunakhonnuruk","sequence":"additional","affiliation":[{"name":"Plant Tissue Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,10]]},"reference":[{"key":"ref_1","unstructured":"Ingkaninan, K. 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