{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T08:47:38Z","timestamp":1785833258131,"version":"3.56.0"},"reference-count":60,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University \u201cG. d\u2019Annunzio\u201d Chieti-Pescara"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Gastric cancer (GC) remains a major global health challenge, ranking among the most lethal malignancies due to late diagnosis, high tumor heterogeneity, and limited treatment efficacy. The search for safer, nutritionally based adjuncts to conventional therapies is therefore a research priority. Donkey milk (DM), traditionally used as a hypoallergenic substitute for infants, is emerging as a functional food with remarkable bioactivity. Its composition closely resembles human milk, with high levels of bioactive proteins, a favorable polyunsaturated lipid profile, antioxidant vitamins, and immune-supportive minerals. Despite its growing nutraceutical appeal, the anticancer potential of DM in GC has not yet been explored. This study represents the first investigation of DM in human gastric adenocarcinoma (AGS) cells. Using increasing concentrations of whole DM (25\u2013100%), a dose-dependent inhibition of cell viability and migration was observed. Mechanistic insights reveal that DM induces mitochondrial oxidative stress, disrupts cell cycle progression (S\/G2 accumulation at 75%, G2 arrest at 100%), and unexpectedly triggers a pro-inflammatory gene signature suggesting stress-driven immunostimulation rather than canonical apoptosis. These findings highlight a non-classical, context-dependent cytotoxic mechanism that distinguishes DM from conventional pro-apoptotic agents. DM may represent a promising nutraceutical candidate for GC management, bridging traditional food resources with modern oncology. By inhibiting hallmark cancer traits while engaging unique immunological pathways, DM offers a sustainable, low-toxicity approach with translational potential. Future studies will focus on the characterization of active components, validation in organoid and animal models, and exploring clinical applications of DM-derived bioactive components in cancer prevention and therapy.<\/jats:p>","DOI":"10.3390\/biology15151279","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T08:02:19Z","timestamp":1785830539000},"page":"1279","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Potential Anticancer Activity of Donkey Milk in Human Gastric Adenocarcinoma (AGS) Cell Line"],"prefix":"10.3390","volume":"15","author":[{"given":"Mariangela","family":"Mazzone","sequence":"first","affiliation":[{"name":"Department of Innovative Technologies in Medicine & Dentistry, University \u201cG. d\u2019Annunzio\u201d Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9308-9857","authenticated-orcid":false,"given":"Maria Carmela","family":"Di Marcantonio","sequence":"additional","affiliation":[{"name":"Department of Innovative Technologies in Medicine & Dentistry, University \u201cG. d\u2019Annunzio\u201d Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Sindaco","sequence":"additional","affiliation":[{"name":"Department of Innovative Technologies in Medicine & Dentistry, University \u201cG. d\u2019Annunzio\u201d Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0207-089X","authenticated-orcid":false,"given":"Antonella","family":"Fatica","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0423-5804","authenticated-orcid":false,"given":"Noemi","family":"Mencarelli","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University \u201cG. d\u2019Annunzio\u201d Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2283-4159","authenticated-orcid":false,"given":"Marialucia","family":"Gallorini","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University \u201cG. d\u2019Annunzio\u201d Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelia","family":"Cataldi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University \u201cG. d\u2019Annunzio\u201d Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaella","family":"Muraro","sequence":"additional","affiliation":[{"name":"Department of Innovative Technologies in Medicine & Dentistry, University \u201cG. d\u2019Annunzio\u201d Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9945-1458","authenticated-orcid":false,"given":"Elisabetta","family":"Salimei","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7127-0938","authenticated-orcid":false,"given":"Gabriella","family":"Mincione","sequence":"additional","affiliation":[{"name":"Department of Innovative Technologies in Medicine & Dentistry, University \u201cG. d\u2019Annunzio\u201d Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"ref_1","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.rxeng.2022.10.011","article-title":"Gastric adenocarcinoma: A review of the TNM classification system and ways of spreading","volume":"65","year":"2023","journal-title":"Radiologia (Engl. Ed.)"},{"key":"ref_3","first-page":"100845","article-title":"Gastric cancer-Epidemiology, modifiable and non-modifiable risk factors, challenges and opportunities: An updated review","volume":"41","author":"Mamun","year":"2024","journal-title":"Cancer Treat. Res. Commun."},{"key":"ref_4","first-page":"140","article-title":"Gastric Cancer: Rapid Evidence Review","volume":"111","author":"Mott","year":"2025","journal-title":"Am. Fam. Physician"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Hou, W., Zhao, Y., and Zhu, H. (2023). Predictive Biomarkers for Immunotherapy in Gastric Cancer: Current Status and Emerging Prospects. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242015321"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/15330338241271935","article-title":"Gastric Cancer Signaling Pathways and Therapeutic Applications","volume":"23","author":"Wu","year":"2024","journal-title":"Technol. Cancer Res. Treat."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/j.trecan.2024.03.008","article-title":"Gastric cancer immunosuppressive microenvironment heterogeneity: Implications for therapy development","volume":"10","author":"Yasuda","year":"2024","journal-title":"Trends Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"101733","DOI":"10.1016\/j.bpg.2021.101733","article-title":"Recent progress in gastric cancer prevention","volume":"51","author":"Park","year":"2021","journal-title":"Best. Pract. Res. Clin. Gastroenterol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1002\/jso.26844","article-title":"Advances in screening and detection of gastric cancer","volume":"125","author":"Xia","year":"2022","journal-title":"J. Surg. Oncol."},{"key":"ref_10","first-page":"101886","article-title":"Advancements in non-invasive diagnosis of gastric cancer","volume":"31","author":"Wang","year":"2025","journal-title":"World J. Gastroenterol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Mao, Q.Q., Xu, X.Y., Shang, A., Gan, R.Y., Wu, D.T., Atanasov, A.G., and Li, H.B. (2020). Phytochemicals for the Prevention and Treatment of Gastric Cancer: Effects and Mechanisms. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21020570"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kang, S.Y., Hwang, D., Shin, S., Park, J., Kim, M., Rahman, M.D.H., Rahman, M.A., Ko, S.G., and Kim, B. (2021). Potential of Bioactive Food Components against Gastric Cancer: Insights into Molecular Mechanism and Therapeutic Targets. Cancers, 13.","DOI":"10.20944\/preprints202107.0612.v1"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/pai.12871","article-title":"Tolerability and palatability of donkey\u2019s milk in children with cow\u2019s milk allergy","volume":"29","author":"Barni","year":"2018","journal-title":"Pediatr. Allergy Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.foodres.2018.04.051","article-title":"Donkey milk as a supplement in infant formula: Benefits and technological challenges","volume":"109","author":"Souroullas","year":"2018","journal-title":"Food Res. Int."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Cresi, F., Maggiora, E., Pirra, A., Tonetto, P., Rubino, C., Cavallarin, L., Giribaldi, M., Moro, G.E., Peila, C., and Coscia, A. (2020). Effects on Gastroesophageal Reflux of Donkey Milk-Derived Human Milk Fortifier Versus Standard Fortifier in Preterm Newborns: Additional Data from the FortiLat Study. Nutrients, 12.","DOI":"10.21203\/rs.3.rs-19809\/v1"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Bertino, E., Agosti, M., Peila, C., Corridori, M., Pintus, R., and Fanos, V. (2022). The Donkey Milk in Infant Nutrition. Nutrients, 14.","DOI":"10.3390\/nu14030403"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/1476-511X-11-113","article-title":"Fat content, energy value and fatty acid profile of donkey milk during lactation and implications for human nutrition","volume":"11","author":"Martemucci","year":"2012","journal-title":"Lipids Health Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.foodres.2017.07.002","article-title":"Proteins and bioactive peptides from donkey milk: The molecular basis for its reduced allergenic properties","volume":"99","author":"Cunsolo","year":"2017","journal-title":"Food Res. Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"104007","DOI":"10.1016\/j.jprot.2020.104007","article-title":"Antibacterial potential of donkey\u2019s milk disclosed by untargeted proteomics","volume":"231","author":"Spada","year":"2021","journal-title":"J. Proteom."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.3168\/jds.2022-22465","article-title":"Invited review: Human, cow, and donkey milk comparison: Focus on metabolic effects","volume":"106","author":"Cimmino","year":"2023","journal-title":"J. Dairy Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.1080\/14786419.2022.2116706","article-title":"Bioactive natural products in donkey and camel milk: A perspective review","volume":"37","author":"Salvo","year":"2023","journal-title":"Nat. Prod. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"116307","DOI":"10.1016\/j.foodres.2025.116307","article-title":"Unlocking the potential of donkey Milk: Nutritional composition, bioactive properties and future prospects","volume":"209","author":"Xie","year":"2025","journal-title":"Food Res. Int."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Di Francesco, L., Di Girolamo, F., Mennini, M., Masotti, A., Salvatori, G., Rigon, G., Signore, F., Pietrantoni, E., Scapaticci, M., and Lante, I. (2018). A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks\u2019 Profiling. Nutrients, 10.","DOI":"10.20944\/preprints201808.0349.v1"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"130397","DOI":"10.1016\/j.foodchem.2021.130397","article-title":"Novel insights into whey protein differences between donkey and bovine milk","volume":"365","author":"Li","year":"2021","journal-title":"Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Martini, M., Altomonte, I., Tric\u00f2, D., Lapenta, R., and Salari, F. (2021). Current Knowledge on Functionality and Potential Therapeutic Uses of Donkey Milk. Animals, 11.","DOI":"10.3390\/ani11051382"},{"key":"ref_26","first-page":"1125","article-title":"Mare\u2019s Milk: Composition, Properties, and Application in Medicine","volume":"76","author":"Musaev","year":"2021","journal-title":"Arch. Razi Inst."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"104006","DOI":"10.1016\/j.jevs.2022.104006","article-title":"A Systematic Review of the Bioactive Components, Nutritional Qualities and Potential Therapeutic Applications of Donkey Milk","volume":"115","author":"Garhwal","year":"2022","journal-title":"J. Equine Vet. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"16435","DOI":"10.1021\/acs.jafc.3c04534","article-title":"Proteomics and Peptidomics As a Tool to Compare the Proteins and Endogenous Peptides in Human, Cow, and Donkey Milk","volume":"71","author":"Ning","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"104225","DOI":"10.1016\/j.jevs.2023.104225","article-title":"Donkey Milk: An Overview of its Chemical Composition and Main Nutritional Properties or Human Health Benefit Properties","volume":"121","year":"2023","journal-title":"J. Equine Vet. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Khan, M.Z., Chen, W., Li, M., Ren, W., Huang, B., Kou, X., Ullah, Q., Wei, L., Wang, T., and Khan, A. (2024). Is there sufficient evidence to support the health benefits of including donkey milk in the diet?. Front. Nutr., 11.","DOI":"10.3389\/fnut.2024.1404998"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Vincenzetti, S., Santini, G., Polzonetti, V., Pucciarelli, S., Klimanova, Y., and Polidori, P. (2021). Vitamins in Human and Donkey Milk: Functional and Nutritional Role. Nutrients, 13.","DOI":"10.3390\/nu13051509"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Mecocci, S., Gevi, F., Pietrucci, D., Cavinato, L., Luly, F.R., Pascucci, L., Petrini, S., Ascenzioni, F., Zolla, L., and Chillemi, G. (2020). Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile. Nutrients, 12.","DOI":"10.3390\/nu12102908"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Mecocci, S., Pietrucci, D., Milanesi, M., Pascucci, L., Filippi, S., Rosato, V., Chillemi, G., Capomaccio, S., and Cappelli, K. (2021). Transcriptomic Characterization of Cow, Donkey and Goat Milk Extracellular Vesicles Reveals Their Anti-Inflammatory and Immunomodulatory Potential. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222312759"},{"key":"ref_34","first-page":"57","article-title":"Anticancer activity of cow, sheep, goat, mare, donkey and camel milks and their caseins and whey proteins and in silico comparison of the caseins","volume":"6","author":"Shariatikia","year":"2017","journal-title":"Mol. Biol. Res. Commun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1080\/10520295.2018.1448112","article-title":"Donkey milk kefir induces apoptosis and suppresses proliferation of Ehrlich ascites carcinoma by decreasing iNOS in mice","volume":"93","author":"Esener","year":"2018","journal-title":"Biotech. Histochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.1039\/C9FO02934F","article-title":"Donkey milk inhibits triple-negative breast tumor progression and is associated with increased cleaved-caspase-3 expression","volume":"11","author":"Li","year":"2020","journal-title":"Food Funct."},{"key":"ref_37","unstructured":"McSweeney, P.L.H., and McNamara, J.P. (2022). Donkey. Encyclopedia of Dairy Sciences, Academic Press."},{"key":"ref_38","unstructured":"AOAC, Association of Official Analytical Chemist (2000). Official Methods of Analysis, The Association of Official Analytical Chemists. [17th ed.]."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Fantuz, F., Todini, L., Salimei, E., Fatica, A., Mariani, P., Marcantoni, F., and Ferraro, S. (2025). Milk Yield, Major Milk Components and Macro Minerals in Blood Serum of Lactating Donkeys, as Affected by Dietary Trace Element Supplementation and Stage of Lactation. Animals, 15.","DOI":"10.3390\/ani15081073"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Savino, L., Di Marcantonio, M.C., Moscatello, C., Cotellese, R., Centurione, L., Muraro, R., Aceto, G.M., and Mincione, G. (2022). Effects of H2O2 Treatment Combined With PI3K Inhibitor and MEK Inhibitor in AGS Cells: Oxidative Stress Outcomes in a Model of Gastric Cancer. Front. Oncol., 12.","DOI":"10.3389\/fonc.2022.860760"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1021\/acsmedchemlett.0c00285","article-title":"Antiglioma Activity of Aryl and Amido-Aryl Acetamidine Derivatives Targeting iNOS: Synthesis and Biological Evaluation","volume":"11","author":"Maccallini","year":"2020","journal-title":"ACS Med. Chem. Lett."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/S0140-6736(20)31288-5","article-title":"Gastric cancer","volume":"396","author":"Smyth","year":"2020","journal-title":"Lancet"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"103841","DOI":"10.1016\/j.critrevonc.2022.103841","article-title":"Characteristics of gastric cancer around the world","volume":"181","author":"Carbajal","year":"2023","journal-title":"Crit. Rev. Oncol. Hematol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2452","DOI":"10.3748\/wjg.v29.i16.2452","article-title":"Updates on global epidemiology, risk and prognostic factors of gastric cancer","volume":"29","author":"Yang","year":"2023","journal-title":"World J. Gastroenterol."},{"key":"ref_45","first-page":"2209","article-title":"Gastric cancer: Adjuvant chemotherapy versus chemoradiation. A clinical point of view","volume":"24","author":"Cainap","year":"2019","journal-title":"J. BUON"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Rao, X., Zhang, C., Luo, H., Zhang, J., Zhuang, Z., Liang, Z., and Wu, X. (2022). Targeting Gastric Cancer Stem Cells to Enhance Treatment Response. Cells, 11.","DOI":"10.3390\/cells11182828"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3711","DOI":"10.2174\/138161207783018590","article-title":"Immunological properties of donkey\u2019s milk: Its potential use in the prevention of atherosclerosis","volume":"13","author":"Tafaro","year":"2007","journal-title":"Curr. Pharm. Des."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"864","DOI":"10.2174\/138161210790883651","article-title":"Donkey and goat milk intake and modulation of the human aged immune response","volume":"16","author":"Amati","year":"2010","journal-title":"Curr. Pharm. Des."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"859","DOI":"10.2174\/138161210790883688","article-title":"Donkey\u2019s and goat\u2019s milk consumption and benefits to human health with special reference to the inflammatory status","volume":"16","author":"Jirillo","year":"2010","journal-title":"Curr. Pharm. Des."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1016\/j.jnutbio.2015.05.003","article-title":"Human, donkey and cow milk differently affects energy efficiency and inflammatory state by modulating mitochondrial function and gut microbiota","volume":"26","author":"Trinchese","year":"2015","journal-title":"J. Nutr. Biochem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e18449","DOI":"10.1590\/s2175-97902019000418449","article-title":"Up-regulation of human immune system function by donkey\u2019s milk","volume":"56","author":"Taghiloo","year":"2020","journal-title":"Braz. J. Pharm. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"671","DOI":"10.5114\/aoms.2016.59645","article-title":"Indomethacin-induced gastric damage in rats and the protective effect of donkey milk","volume":"14","author":"Tastekin","year":"2018","journal-title":"Arch. Med. Sci."},{"key":"ref_53","first-page":"e13935","article-title":"Effects of donkey milk on oxidative stress and inflammatory response","volume":"46","author":"Li","year":"2022","journal-title":"J. Food Biochem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3851478","DOI":"10.1155\/2022\/3851478","article-title":"Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare\u2019s Milk in the Treatment of Dysbiosis","volume":"2022","author":"Kozhakhmetov","year":"2022","journal-title":"Biomed. Res. Int."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e70156","DOI":"10.1002\/vms3.70156","article-title":"Protective Effects of Donkey Milk on Ethanol-Induced Gastric Ulcer in Rat","volume":"11","author":"Sami","year":"2025","journal-title":"Vet. Med. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.idairyj.2009.05.007","article-title":"Anti-proliferative and anti-tumour effect of active components in donkey milk on A549 human lung cancer cells","volume":"19","author":"Mao","year":"2009","journal-title":"Int. Dairy J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"4823","DOI":"10.1039\/C8FO02584C","article-title":"Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2\/M growth arrest via the p38 pathway in HT-29 cells","volume":"10","author":"Wang","year":"2019","journal-title":"Food Funct."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"112221","DOI":"10.1016\/j.jep.2019.112221","article-title":"Dezhou donkey (Equus asinus) milk a potential treatment strategy for type 2 diabetes","volume":"246","author":"Li","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"5618127","DOI":"10.1155\/2020\/5618127","article-title":"The Regenerative Potential of Donkey and Human Milk on the Redox-Sensitive and Proliferative Signaling Pathways of Skin Fibroblasts","volume":"2020","author":"Kocic","year":"2020","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.3168\/jds.2024-25388","article-title":"Donkey milk supplementation alleviates renal fibrosis of chronic kidney disease by enhancing anti-inflammatory ability","volume":"108","author":"Huimin","year":"2025","journal-title":"J. Dairy Sci."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/15\/1279\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T08:38:47Z","timestamp":1785832727000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/15\/15\/1279"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":60,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["biology15151279"],"URL":"https:\/\/doi.org\/10.3390\/biology15151279","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,4]]}}}